Difference between revisions of "Os04g0677500"
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Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase, EC 2.7.1.40) is a key regulatory enzyme of the glycolyticpathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.This enzyme is critical for the control of the metabolicflux through the glycolytic pathway. Moreover, its substrate (PEP) and product (pyruvate) are both involved in a variety of metabolic processes. Thus, PK plays a key role in the cellular metabolic flux <ref name="ref2" />.The role of PK in plant growth and development is an interesting subject, because growth and development are closely linked to agricultural economy<ref name="ref3" />.Pyruvate kinase (PK) catalyzes the final step of glycolysis.Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase,EC 2.7.1.40) is a key regulatory enzyme of the glycolytic pathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.<ref name="ref3" />. | Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase, EC 2.7.1.40) is a key regulatory enzyme of the glycolyticpathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.This enzyme is critical for the control of the metabolicflux through the glycolytic pathway. Moreover, its substrate (PEP) and product (pyruvate) are both involved in a variety of metabolic processes. Thus, PK plays a key role in the cellular metabolic flux <ref name="ref2" />.The role of PK in plant growth and development is an interesting subject, because growth and development are closely linked to agricultural economy<ref name="ref3" />.Pyruvate kinase (PK) catalyzes the final step of glycolysis.Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase,EC 2.7.1.40) is a key regulatory enzyme of the glycolytic pathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.<ref name="ref3" />. | ||
the cellular metabolic flux.PK has been purified and characterized from various organisms. Most bacteria and lower eukaryotes have one form of PK, although two forms of PK have been isolated from Escherichia coli.In plants, PK is subject to a number of regulatory mechanisms, including allosteric control by various metabolite effectors such as Glu and Asp,and phosphorylation and ubiquitination<ref name="ref3" />. | the cellular metabolic flux.PK has been purified and characterized from various organisms. Most bacteria and lower eukaryotes have one form of PK, although two forms of PK have been isolated from Escherichia coli.In plants, PK is subject to a number of regulatory mechanisms, including allosteric control by various metabolite effectors such as Glu and Asp,and phosphorylation and ubiquitination<ref name="ref3" />. | ||
| + | PK activity is controlled by several physiological effectors, including Hq, Mn2q, Kq and Mg2q . Furthermore, the enzyme displays sigmoidal kinetics toward PEP and it is activated by a heterotropic effector whose nature depends on the organism<ref name="ref4" />. | ||
===Expression=== | ===Expression=== | ||
Revision as of 11:59, 8 June 2014
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Contents
Annotated Information
Function
this gene named Os04g0677500 is located in rice CHR 4:35005101-35008954,which the length of this gene is 3854bp.the expression of this gene is Rice mitochondrial Pyruvate kinase [1].
Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase, EC 2.7.1.40) is a key regulatory enzyme of the glycolyticpathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.This enzyme is critical for the control of the metabolicflux through the glycolytic pathway. Moreover, its substrate (PEP) and product (pyruvate) are both involved in a variety of metabolic processes. Thus, PK plays a key role in the cellular metabolic flux [2].The role of PK in plant growth and development is an interesting subject, because growth and development are closely linked to agricultural economy[3].Pyruvate kinase (PK) catalyzes the final step of glycolysis.Pyruvate kinase (PK; ATP:pyruvate 2-O-phosphotranferase,EC 2.7.1.40) is a key regulatory enzyme of the glycolytic pathway. It catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) toATP and pyruvate by essentially irreversible transphosphorylation.[3].
the cellular metabolic flux.PK has been purified and characterized from various organisms. Most bacteria and lower eukaryotes have one form of PK, although two forms of PK have been isolated from Escherichia coli.In plants, PK is subject to a number of regulatory mechanisms, including allosteric control by various metabolite effectors such as Glu and Asp,and phosphorylation and ubiquitination[3].
PK activity is controlled by several physiological effectors, including Hq, Mn2q, Kq and Mg2q . Furthermore, the enzyme displays sigmoidal kinetics toward PEP and it is activated by a heterotropic effector whose nature depends on the organism[4].
Expression
using bioinformatics methods to scan rice genome, we get 10 with PK extremely conservative regions (PK - PK - C) gene.Os04g0677500 is one of that Homologous genes
,and it was highly expressed and composed of sexual expression [1].OsPK1 was expressed mainly in leaf mesophyll cells, phloem companion cells in
stems, and cortical parenchyma cells in roots[2]. the Representative sequence Os04g0677500 is AK100393
this gene expressed in different Organs and tissues of rice , and their Feature Number is 9888 21058 422901. their expression as following pictures show.
.
Quantitative RT-PCR and GUS staining analyses exhibited that OsPK was expressed mainly in leaf mesophyll cells, phloem companion cells in
stems, and cortical parenchyma cells in roots.The transcriptions of four other putative enzymes involved in the glycolysis/gluconeogenesis pathway were altered in ospk1. The amount of pyruvate is decreased in ospk1. We propose that OsPK1 plays an important role through affecting the glycolytic pathway. The contents of glucose and fructose were markedly accumulated in flag leaf blade and panicle of ospk1. The sucrose level in panicle of ospk1 was decreased by approximately 84%. These findings indicated that both monosaccharide metabolism and sugar transport are altered due to the decreased expression of OsPK1.Together, these results provide new insights into the role of PKc in plant morphological development, especially plant height. The transcriptions of four other putative enzymes involved in the glycolysis/gluconeogenesis pathway were altered in ospk[3].
Evolution
a single T-DNA was inserted in the transcriptional regulatory region of OsPK1 in ospk1. Quantitative RT-PCR result showed that OsPK1 expression was decreased by approximately 90% in ospk1 compared with that in WT [2].There are 4 homologous genes in the Pyruvate kinase family, they are Os04g067750, Os10g0571200,Os01g0276700 and Os03g0672300 .by MEME program analysis show that the family has completely conservative sequences,basically the same the order of sequences,The diversity of four mtOsPKs expression in rice may be adapted to different environment in evolution[1].
Labs working on this gene
- Institute of microbiology ,Chinese academy of sciences.
- International Rice Research Institute, Manila, DAPO Box 7777, Philippines
- Oryza sativa Japonica Group (cultivar: Nipponbare)
References
Structured Information
| Gene Name |
Os04g0677500 |
|---|---|
| Description |
Pyruvate kinase (EC 2.7.1.40) |
| Version |
NM_001060800.1 GI:115461329 GeneID:4337406 |
| Length |
3615 bp |
| Definition |
Oryza sativa Japonica Group Os04g0677500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:35005323..35008937 |
| Sequence Coding Region |
35005655..35006467,35007045..35007506,35008380..35008640 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:35005323..35008937 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:35005323..35008937 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcgaacatcgacatggggaagatcctggcggggctggagaacgacgacgcgcgggtgcccaagaccaagctggtctgcacgctcggcccggcctcccgctccgtccccatgctcgagaagctgctccgcgccgggatgaacgtcgcgcgcttcaacttctcccacggcacccacgagtaccaccaggagaccctcgacaacctccgccaggccatgcacaacaccggcgtcctctgcgccgtcatgctcgataccaagggtcctgagattcgtactggatttttgaaggatggcaagccaatcaagctaacaaagggtcaagaactcactgttaccaccgattatgagatcaagggtgatgagaacatgattaccatgagttacaagaaactgccagttgatgtgaagcctggaaatgtcattctctgcgccgatggtacaatctctttgactgttttgtcctgtgatccaaaggctggaactgtgaggtgtaggtgtgagaacacagcaatgcttggcgagagaaagaattgcaatctgccaggaattgttgtggaccttcctacactgactgagaaggataaagaagacattttgggatggggtgtgccaaatgacatagacatgattgctctgtcgtttgtccgtaaaggatcagatttggttaccgtcagacaacttcttggacagcatgcaaagcgcatcaagctgatgtcaaaggttgaaaaccaagagggtgttgtaaacttcgatgagatcttgagggaaacggatgcatttatggttgctagaggtgatcttggaatggagattccagttgagaagatattccttgcacagaagatgatgatttacaagtgcaaccttgctggaaagcctgttgtgactgctactcagatgcttgagtcgatgatcaaatcaccacgtccaactcgtgctgaggcaactgacgttgcaaatgcagttcttgatggaactgactgcgtcatgcttagtggagagagtgctgctggagcataccctgaagtagctgtgaagatcatggcacgtatatgtgttgaggcagagtcttcccttgacaacgaagctgtcttcaaggagatgatcaggtctgcgccccttccgatgagcccattggagtctctcgcatcctctgctgtacgcacagccaacaaggccaaggcagccctgattgttgtcttgactcgtggtggtaccacggcaaagctggttgccaagtatcgtcccagggttccaatcctctctgtggttgtccccgtgttgacaaccgattcattcgactggacaatcagctcggagggcccagcaaggcacagcctaatctacagaggtcttgttcctctcctggctgagggttctgccaaagccaccgattcggagtcgacagaggtcatccttgatgctgctctcaagtcagctgtacagaagcagttgtgcaagcctggtgatgctgttgttgctctgcaccgtattggcgtcgcatccgtgatcaagatctgcatcgtgaagtaa</cdnaseq> |
| Protein Sequence |
<aaseq>MANIDMGKILAGLENDDARVPKTKLVCTLGPASRSVPMLEKLLR AGMNVARFNFSHGTHEYHQETLDNLRQAMHNTGVLCAVMLDTKGPEIRTGFLKDGKPI KLTKGQELTVTTDYEIKGDENMITMSYKKLPVDVKPGNVILCADGTISLTVLSCDPKA GTVRCRCENTAMLGERKNCNLPGIVVDLPTLTEKDKEDILGWGVPNDIDMIALSFVRK GSDLVTVRQLLGQHAKRIKLMSKVENQEGVVNFDEILRETDAFMVARGDLGMEIPVEK IFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSG ESAAGAYPEVAVKIMARICVEAESSLDNEAVFKEMIRSAPLPMSPLESLASSAVRTAN KAKAALIVVLTRGGTTAKLVAKYRPRVPILSVVVPVLTTDSFDWTISSEGPARHSLIY RGLVPLLAEGSAKATDSESTEVILDAALKSAVQKQLCKPGDAVVALHRIGVASVIKIC IVK</aaseq> |
| Gene Sequence |
<dnaseqindica>2471..3283#1432..1893#298..558#acctcgaccctctgcccctcaactcggtggtgaccacgcgtccacatctctcatctttctttctccgctattctcctccagtcctcttctcttcttcctccctcccttcgcccactgaatcgccaaaaaaattcccctcctccactccatctctataaaaatcctcccccaaatctcgcctcgtttagccagcacaactcacacaactctctctctctctctctctctctctctctcgtcgtctgcaagcttccgcctcccggatcgcggcgaccaccggaggaggagggagcagccatggcgaacatcgacatggggaagatcctggcggggctggagaacgacgacgcgcgggtgcccaagaccaagctggtctgcacgctcggcccggcctcccgctccgtccccatgctcgagaagctgctccgcgccgggatgaacgtcgcgcgcttcaacttctcccacggcacccacgagtaccaccaggagaccctcgacaacctccgccaggccatgcacaacaccggcgtcctctgcgccgtcatgctcgataccaaggttaccccctcactgctttgcttgtcgatctctacatgcttgtatgctgctgggttgttcggatcggaccagtcggggtagcttgcgtttgatacagagcggatttcgatttggatctgctcgtcttttttatcgggagaagctgggtttgatttggaggcagcatggtaggattatgagttgagtttacagcggtttgactttggaggactcgacaaaatggtatccggggttagatttgcctgcgcttgcgcttttgccttgtcgcagatggctggatcttggattgggggctcaggcttgcgtctccatgtgggtagttagttttggagtcttatggattctgagcaagtgtaagtgtgtaactggttcgtagtgtgatccgtttgcggtgttggtgagattgatgagctgcgtgcatcttgtttgtcatatttagctttacagttcgtctctatgctttttcaagttaatttattcatttgtagagttggattggaaggatggttaacagatcttgggatcggtggctttggagtatggagtgttcaaatcctagtgttctgattttgttgcttttgtaggaggatatgattaacacgtaaaagaaggctttattagtgtagtcatttcttctattatgccttgatgtttctgtcatgcacgtaactattggtgcttatctttttacactctcacacttcatggtttgctttgtagttcaaataagttacttacagaattgtactgcacacagtttatcttttttatcacttcttgtactgcaattagttccattatttttacctgtttagtagcatatttaaggcgattctgtggtgactgttctgaacatttcattcattgcttcagggtcctgagattcgtactggatttttgaaggatggcaagccaatcaagctaacaaagggtcaagaactcactgttaccaccgattatgagatcaagggtgatgagaacatgattaccatgagttacaagaaactgccagttgatgtgaagcctggaaatgtcattctctgcgccgatggtacaatctctttgactgttttgtcctgtgatccaaaggctggaactgtgaggtgtaggtgtgagaacacagcaatgcttggcgagagaaagaattgcaatctgccaggaattgttgtggaccttcctacactgactgagaaggataaagaagacattttgggatggggtgtgccaaatgacatagacatgattgctctgtcgtttgtccgtaaaggatcagatttggttaccgtcagacaacttcttggacagcatgcaaagcgcatcaagctgatgtcaaaggtttgtaatcattatcactattttttgggtcctgttaggttttcagttccacagtttgcttaagtatttaattaccaggacggttgagtgacaatacttctcagttagatgctttaattaatgttttgttgtccctttctggtatatactggagccagaattgtttggtctctgcctttgtacattgaccatttgacccctttttgtgctatattaggtaataaaatgcaattttgttccttttctatgtgcttgaacaactagtttcagaataaccttttttcacgtttgcttgttcaaatagcagctacttattttctcatgcactttcactgatagggtttagcttgttgcctgatatgcattaaaatgaggaattcaggttattgcatttctttgctgctaactgaaattttggatctagatggttcaccgtgttataaatgacctgtcttactgccatgatgataaaatatattcgtgatgatgataatacatatgaggtgcctggtaatttaccatgctggttatgttgctgtgcgtctaatttctttcttaactttttccttgtttttaggttgaaaaccaagagggtgttgtaaacttcgatgagatcttgagggaaacggatgcatttatggttgctagaggtgatcttggaatggagattccagttgagaagatattccttgcacagaagatgatgatttacaagtgcaaccttgctggaaagcctgttgtgactgctactcagatgcttgagtcgatgatcaaatcaccacgtccaactcgtgctgaggcaactgacgttgcaaatgcagttcttgatggaactgactgcgtcatgcttagtggagagagtgctgctggagcataccctgaagtagctgtgaagatcatggcacgtatatgtgttgaggcagagtcttcccttgacaacgaagctgtcttcaaggagatgatcaggtctgcgccccttccgatgagcccattggagtctctcgcatcctctgctgtacgcacagccaacaaggccaaggcagccctgattgttgtcttgactcgtggtggtaccacggcaaagctggttgccaagtatcgtcccagggttccaatcctctctgtggttgtccccgtgttgacaaccgattcattcgactggacaatcagctcggagggcccagcaaggcacagcctaatctacagaggtcttgttcctctcctggctgagggttctgccaaagccaccgattcggagtcgacagaggtcatccttgatgctgctctcaagtcagctgtacagaagcagttgtgcaagcctggtgatgctgttgttgctctgcaccgtattggcgtcgcatccgtgatcaagatctgcatcgtgaagtaatcgctacacggtgtcttcttagcgccattttgctgggttaagtggcaaaacaaaaactgctatgcttgtttttactcccatagtccatctttgggagacaatttaattaccgtatcagcccctgcagttcgcagggtgcctttcgttacatagatttgctggtaaaatgaggagagatagaactccattatgtagagctctatttatcgtcgatgccatgtagtatggcaggtggtttctattccatgcctgcacaattttctggtagctactgtgttgtttgattactgttttttcaggtgatgtcgatggagttgataataatattggca</dnaseqindica> |
| External Link(s) |
- ↑ 1.0 1.1 1.2 1.3 .吴金山1， 张艳2， 朱传凤2， 罗丽娟1.4 个水稻线粒体丙酮酸激酶基因（mtOsPKs）表达分析.热带作物学报.Vol．31 No．5.
- ↑ 2.0 2.1 2.2 2.3 Yan Zhang • Wenkai Xiao • Lijuan Luo •Jinhuan Pang • Wei Rong • Chaozu He, Downregulation of OsPK1, a cytosolic pyruvate kinase, by T-DNA insertion causes dwarfism and panicle enclosure in rice.Planta (2012) 235:25–38.
- ↑ 3.0 3.1 3.2 3.3 3.4 Yan Zhang • Wenkai Xiao • Lijuan Luo • Jinhuan Pang • Wei Rong • Chaozu He.Downregulation of OsPK1, a cytosolic pyruvate kinase, by T-DNA insertion causes dwarfism and panicle enclosure in rice.Planta (2012) 235:25–38.
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedref4
