Difference between revisions of "Os04g0580700"
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===Expression=== | ===Expression=== | ||
| − | + | RT-PCR analysis revealed that faint expression of MADS17 was detected at the 0.5-mm stage and established after the 1-mm stage.MADS17was expressed in palea, lodicule, and pistil and weakly in empty glumes and stamens, in addition to MFO1. However, in contrast with MFO1, MADS17 was expressed in the lemma. . At stages Sp3–4, when the lemma and palea initiate, MADS17 was expressed in the floral meristem At stage Sp7, MADS17was expressed in the lodicule and palea. In addition, MADS17 was expressed in the lemma, in which MFO1 was not expressed. At stage Sp8, MADS17 was expressed in the receptacle, lodicule, and ovule but not in the mrp. In total, MFO1 was expressed in areas in which morphological abnormalities were observed, and MADS17 expression generally overlapped that of MFO1. the MADS17 transcript levels in both mfo1-2 and MFO1i plants were slightly higher than in the wild type. This observation raises the possibility that upregulated MADS17 masks the effect of the MFO1 knockdown. Therefore, to examine the potential effect of MADS17 on floral development, we generated 16 MADS17 RNAi (OM17i) plants in which the expression of MADS17 was specifically downregulated. | |
===Evolution=== | ===Evolution=== | ||
Revision as of 02:58, 9 June 2014
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Contents
Annotated Information
Function
MADS17 is a member of rice AGL6-like gene. MADS17, caused no morphological abnormalities in the wild-type background, but it enhanced the phenotype in the mfo1 background, so MADS17 has a minor but redundant function with that of MFO1. MADS17 plays minor but redundant roles with MFO1 in floral development and that its minor functions may not be observed unless the function of MFO1 is severely impaired. To further confirm the role of MADS17, introduced the MFO1i+OM17i double-RNAi cassette into mfo1-2. In these mfo1-2–based double-RNAi plants, although the MFO1expression levels were comparable to those in the MFO1i mfo1-2 plants, the MADS17 expression levels were significantly lower than those in MFO1i mfo1-2 plants.
Expression
RT-PCR analysis revealed that faint expression of MADS17 was detected at the 0.5-mm stage and established after the 1-mm stage.MADS17was expressed in palea, lodicule, and pistil and weakly in empty glumes and stamens, in addition to MFO1. However, in contrast with MFO1, MADS17 was expressed in the lemma. . At stages Sp3–4, when the lemma and palea initiate, MADS17 was expressed in the floral meristem At stage Sp7, MADS17was expressed in the lodicule and palea. In addition, MADS17 was expressed in the lemma, in which MFO1 was not expressed. At stage Sp8, MADS17 was expressed in the receptacle, lodicule, and ovule but not in the mrp. In total, MFO1 was expressed in areas in which morphological abnormalities were observed, and MADS17 expression generally overlapped that of MFO1. the MADS17 transcript levels in both mfo1-2 and MFO1i plants were slightly higher than in the wild type. This observation raises the possibility that upregulated MADS17 masks the effect of the MFO1 knockdown. Therefore, to examine the potential effect of MADS17 on floral development, we generated 16 MADS17 RNAi (OM17i) plants in which the expression of MADS17 was specifically downregulated.
Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os04g0580700 |
|---|---|
| Description |
MADS box transcription factor MADS17 |
| Version |
NM_001060184.1 GI:115460097 GeneID:4336762 |
| Length |
5870 bp |
| Definition |
Oryza sativa Japonica Group Os04g0580700, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:29706752..29712621 |
| Sequence Coding Region |
29706874..29707070,29709019..29709103,29709507..29709568,29709654..29709753,29710457..29710498 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:29706752..29712621 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:29706752..29712621 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggatcgatcagagatggggaggggaagggttgagctgaagcgcatcgagaacaagatcaaccggcaggtcaccttctccaagcgccgcaacggcctcctcaagaaggcgtacgagctctccgtgctctgcgacgcggaggtggcgctcatcatcttctccagccgtggcaagctgtacgaattcggtagcgccggaataaacaagacattggaaaagtacaatagttgctgttacaacgctcaaggttcaaatagtgctcttgctggtggtgaacatcagagctggtaccaagagatgtcaaggctcaagactaagcttgaatgtctccaacgctctcagaggcacatgcttggtgaagatcttggaccattgagcataaaggaactgcagcagctggagaagcaacttgagtactcactgtcacaggctcgacaacgaaagacacaaatcatgatggagcaggtcgacgatcttcgccggaaggaacgccagcttggagagctcaataagcaactgaaaaacaagctagaagctgaagccgatagcagcaactgcagatcagccatccaggattcctgggtccatggcaccgtcgtcagtggcggcagagtgctgaatgctcaaccaccaccagatattgactgtgagcctactctgcaaattgggtactatcaatttgtccgtcctgaggcggccaatccaagaagcaatggaggaggaggggatcagaacaacaactttgtgatgggatggcccctctga</cdnaseq> |
| Protein Sequence |
<aaseq>MDRSEMGRGRVELKRIENKINRQVTFSKRRNGLLKKAYELSVLC DAEVALIIFSSRGKLYEFGSAGINKTLEKYNSCCYNAQGSNSALAGGEHQSWYQEMSR LKTKLECLQRSQRHMLGEDLGPLSIKELQQLEKQLEYSLSQARQRKTQIMMEQVDDLR RKERQLGELNKQLKNKLEAEADSSNCRSAIQDSWVHGTVVSGGRVLNAQPPPDIDCEP TLQIGYYQFVRPEAANPRSNGGGGDQNNNFVMGWPL</aaseq> |
| Gene Sequence |
<dnaseqindica>123..319#2268..2352#2756..2817#2903..3002#3706..3747#5108..5149#5309..5448#5536..5632#tagatcatatctcatcccatcatcggtcatattctcctcagcttcatgttgctnagctataggcgaatggttgacaagagtactagttagcttaataatatagctttgcaaaagttgaggnaatggatcgatcagagatggggaggggaagggttgagctgaagcgcatcgagaacaagatcaaccggcaggtcaccttctccaagcgccgcaacggcctcctcaagaaggcgtacgagctctccgtgctctgcgacgcggaggtggcgctcatcatcttctccagccgtggcaagctgtacgaattcggtagcgccgggtaagcgtgtgcatgatccgttcagtaccctaatcgtatgtgattcttgcagatctggtgattaatttctctcttccctttggtttgttttgtttccttccatgggatgcgtattctcgtttgcttcctcagcttaaatccgaaattgtttcttagattttttgttaccaagtatatatatgtgctcgctggtttattttgctatatggttttttgtttctcgctagcgtgcatgtgttttcatacatcttttaggcttaggtaatcttgccggatgtatgcctagctcttgctacttgcatacttgggtaatcttttcataatgaaaatcctccagccctaactcaataaagcacataatatctcttcttcttcttcttcttcagaacctaaagagcattgatttctttcattcgtgcgtgtgcccggcgtgcgcatgtcaggagcagctaggtatatgaggctctgaggcgcaagttttctgattgcaagagtgacttgaaggggaaagtgatggggtctgctgtttccatttagatgccgcaccaagaactttgtttaaatcttttttttcagaattattgcctaatttgatatccatatatttcattcctgcaagtatataacatcttctcttgccatctctcaggtaaattatattacatatgaaccatgcatatgcctctcttttcttttgatgcatttgtgttttcctgaaaggttcgtgttcccgtttaaataaagctttcagatttttttcctgataggtacatgagtttttttttcatttatttgattaaaccctcaaacattatcatcactttagctgcatatatatgaattcgatctttcaaatctcttatgtgtatacgctcttttgcaagattttgtgattctggtattattatttttttcttgttgcattgcctcttgctaatacatgaaggtaaccgagaggggcttatgcacattttgagttgaatgttagtaattgctatatgtattgacgggaaaatcataaatatatatagacgaaaaataactgtgttgattgaaatgcatggatcgaattgaacaccgtttagttgtggaaattcttttatttcttctccggtgatcagtgtgtagggcttctctccagggaccatcagagcaggcgggccatgaaaaaaattttgaataaagtaaatacattacgaaagagttttgtgttattgtgaccggcaactcttgttgctgaacttgctgcttgaacctggtatgcacatgaatatggtggtttttctttttttcatcatagtttattttgcaacatttactttggagtcgccaagaacacacatataaagctttacttagaaatattctttttatttgctaataagtgattcggataagcatatgaataagcgaaacaatgaggctttgtgtccgaattagagcatcattaaacaatttctgaaaacaatttcttcagtagcgatgatcgaaagggattcgttgaactctaacaattattaaaattgttcttgaccacaaattcacctggccatatgattatcattacctattttctgcaattaaatttatgtattttcattctgttatattagtctcctgctgtttaaaattacatatcattctaccttgtcctaatttatgtcaaacatttctatccttgacagtcaatttctagaaatttgtataagttaacaacatgcgaattatatattacaaaagtatttctcatgatgaatctaaaaacataaatcttatgatattaaactatatgaaattttaaattttgatggcaaaacgtacaaatgtttaacttagaacaaaactagaatgcaagtaatttcaaatgcagggcgaagagtagattttattgcattcggctttatgtatgtgagtaagtaacatatcctaaacctatttttgtttgcagaataaacaagacattggaaaagtacaatagttgctgttacaacgctcaaggttcaaatagtgctcttgctggtggtgaacatcaggtattcatagaatagttgtcaaacacacttcggtaaccatattatgtagaatcctttaaagttcaaatatacatatctagacctctttgccagtaatattgtctcaaattcttgagatgcaaggaggatcaatgaagagatatatagtacttaaatattgctgattttaatttgaagattgcaatcaaattctaatatatgtactagttcagtgaataagatattagtacttacattttctaaacctaatgataatatcgcttttttccttgatgcaataaaaattaaacattcagattcatataaaataaaattcaagggtaaataagaacaaaaataaatgcttactattttggattttatcaattattggttttagttttttttcccatttgacccatagagctggtaccaagagatgtcaaggctcaagactaagcttgaatgtctccaacgctctcagaggtactgcactgcatcagctgtttcaggaacacttgaaatgcttcaccacttgaatgtttgggtgttatatttgcatatacaacaggcacatgcttggtgaagatcttggaccattgagcataaaggaactgcagcagctggagaagcaacttgagtactcactgtcacaggctcgacaacgaaaggttatatcaccacacaataaaataagtgtagttccttcaattaccttgttaattaacatacaataatatctgctattatctagtacaatactgtatgcattattctatatatacaacctatatcttgacaaataaaagggatatatactgcaaatatatccatctgatgaattgtaagaaattaaccacttaaggccgtaaatggtgctaaaaaagacaacgcagccgtaatatatataagcaacaaatattgcatgagtgtacgtacttgatcaacaaattttacggcgatctctgctggtagtaggatcaatatctggacctttcatctttacctatccaatatatatttatattactaacttaaccattggtagtagaatattttgtctataggtagtaaaattcataaatcaactgtatataatttctactgataataaaataatatcaataaatatcacaattaataaaagtacttgattgagcaccaagtttatttctataataaaaagacaccataagttttctataattgcttttgcttccttccaacttcggggggacatggtttatatgctcctatgccctgaaatactaaatatgtcagaagaagaagacaacaaagtacagggcttaacttgcatgctcgtccatataaaagttatctaaattttagttgagttctttgtatttatttcagacacaaatcatgatggagcaggtcgacgatcttcgccggaaggtattggaatatctgtcaaatgttgtcgcttttctataaatctgtaacatcgtaattaatcatatgatcaagattactaaaccatatatttgtatgatatgatagcttatcagatctagataattaatcctgaaggttgtggacgtattgctttataattcaatacttgttgcttcagatatggggaatttcaagtgcaccacagagttcgaggacatcaaacattatgctgccattttttcagaatctttcctaaatatttatatatgctttggttattactagggtattccgtgaggaactatatattctctcctttagatgagaatgtatttgttcctgattttactcaatcgtaaaaaaaaaaaggtttatacctaggaaaataaatgtaacaccttataatacagatgcccatctgagtcgctgcgtccgtacctacatttcatacggctttaatgtttatgcgttaatttttcaagttgcaatatggccaccaactattaattaaaaagggatttattttgtcaattatcagaacgaattattgaatcttatcaagaatgaattaattacataccaatattatctatcatgtcacaaaatcattatgatcacaaggagctgtttgcaagacttttagggttaattctttttttcttgctatggctaggggtgatagctgattaaggtccatggatttttttacatccgtatctagcatttgtatatttttagatccagtcttcaaattttatagactaaaaacttgggccaattagcaccctagctgtggctcatatgttgctgcatatactctctttatatatatatatgtgtttgatggtatttacttttaaacataaccattcatattattgaaagaaccgtgcaaatatatagaaatataagtcatgtctaaagtacaaacaaaatggaatagaaggatgaatggtcaaatttatgttaaaaagtcaatggcattagacaaaaaagaaataaagggagtatacatctacctatctagctggaactccaagttctattgtctggaaattggaataggctagctcttgctgacctgtttaactggctttggtggggtgctggtgttccatatgtaatggggatgacattacaccaaaatcgctcagcctttctgcttaatttgcttagtaggagtagtgtagtactcctgaacttgaaccctgtagcatgtcgtttctgataatagaagcgggacagagtgttatgtttttctatcgaaaaaataaaatatagttaatcacatctaataatcctgcctcaaaagtgctagttgtatattccctagttgactgattatctgggctgcctgcaggaacgccagcttggagagctcaataagcaactgaaaaacaaggtcctacttatcgtttccacaattaatttctgcctttcaatttggtggattgggaatactgtacaaccacttctctgttttctagtgttaattagtgtatcttcgaattttgtcatgtcatttcgccatgtagtactaacacatcgccacaccattcagctagaagctgaagccgatagcagcaactgcagatcagccatccaggattcctgggtccatggcaccgtcgtcagtggcggcagagtgctgaatgctcaaccaccaccagatattgactgtgagcctactctgcaaattgggtaagtttggaagatgaatcaaaactaaaacctgatcaatctgatcttccgaaccaacgtgtttgaaaatatgcatgcacgctacaggtactatcaatttgtccgtcctgaggcggccaatccaagaagcaatggaggaggaggggatcagaacaacaactttgtgatgggatggcccctctgaagtccaagcttgctaataaaaacgctggcgtgctataatatatagttcggcaaatgttgaaacacatgtgttctcagtatgtatatttctcccctatccggtggtactcttagcacgtacctatgagtgtatgtttgtacgttatctatactctatgctaggcaaccctatactattatggtaagacgtctatcttttcaatctatctatacgtaccatactaattggcaaattttgt</dnaseqindica> 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