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| Line 1: |
Line 1: |
| | >gi|19422258|gb|AF465255.1| Oryza sativa cultivar Nipponbare gibberellin-20 oxidase (Sd-1) gene, complete cds | | >gi|19422258|gb|AF465255.1| Oryza sativa cultivar Nipponbare gibberellin-20 oxidase (Sd-1) gene, complete cds |
| − | CTAGATCAGAGCACACAGAGAAAAAAATATAGACACCTTGGAAATTTGTCACAAAGAGACAAGGTGACTC
| + | Structured Information |
| − | AACAGGCCCTCCAAACTGAAAATTTAATTACTTGCTCAAGATTTAAATATAACTACCCATCCAGTTTTTA
| + | |
| − | ATATATAAAGTTGTTAACTTTTAAACATATATATGTTTCACTGTTCTTATAATGTATTTTATCATTAAAC
| + | Gene Name |
| − | ATACTTTAAAACATATGGCTTATGTTTTTGAATATTTATATTAAAAATTTTAAATAAGATTAATGATCAA
| + | Os01g0883800 |
| − | ACGTATATTTACTAGTTAACGACATCATGTATTAAAAATCGGAGGAGGTATAGAAGTATGTTCTCCTTTC
| + | Description |
| − | TTGTAAACATAGGTTGATCTGTATATTTGTTTTTGTCTTATTTTGTTTTTTCATTGATCTCACCATTAAA
| + | 10322 bp DNA linear PLN 15-FEB-2008. |
| − | CAGGTGGCCTCCAAAAATGCATGCAGCCATGTATCTTCCCAGTCCATGAAATTAATCTTGAATTATTATA
| + | Version |
| − | AATTAAAAACATATTAGGATTTGATATATGAAAGGTATAATGGTAGCAGATCTATCATAGAAAACCTATA
| + | AB013448.1 GI:4521189 |
| − | ACACGTAGATGACCGAGTAGAGAAAAAAGATATACCCAACATAATCAAGTACCTTGTTAATTAGTAAGAA
| + | Length |
| − | GTAAGAAAACCATATAAATACAAGCATTTGGGTGAAGCTAGAATGGGAACTATATTACCATGTATCCCAT
| + | 10322 bp |
| − | ACATATCTATTACGCACTTAATACCTGAATTATTCGTGTAACGAAGAACATGCTTTGGTAAAAAATAAAA
| + | Definition |
| − | TATTTGGACCGTATACCATGCGGTTATCGCAGTTATCACGTGCGGTAATATTCTCAGTTTTTCAAACCGC
| + | Oryza sativa Japonica Group gene for Pib, complete cds. |
| − | GGTATAACTTGCAATAACCGCGTGGTTTTCGCGGTAACCACCAAACCGTGGGGCTGTGGTAACCCCACCT
| + | S常规信息 |
| − | AAAACGATTTGGTAAACCCTAGTCAGAGCTAGCTTGATCGTGGCTCCGCCCTCTGCATCTCCTCATGGTC
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| − | ACAAGATCAATTTAGAACCTCTTATAAGCTGTTGAACCATCAGTACTACAGTCCCAAGATTAACATATTT
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| − | TTTAAAATATCAAAATTGCTCATGATGAATTGATTACCGTTCATGTGCCTGTATGGTGCATGGGTGGTAT
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| − | AGTGCACCGTGACCTGTACTGTGCTAGATGTTCCTTCCAACCTTAGTACTTCACGAAAAGGAGAGGAACC
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| − | ATTTCCCTGTCACCTTCCTGCAACATGGTCAGGCATAAACCAAACACGATCGAAGCGAATCGGCGATACC
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| − | ACACAATAGCCGCGCGTCGCAAACAACAATTCCGCGGCTAGCTACTTCCGACCTCCGAAACTACTGCGAG
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| − | CCCAAGTGGGTACGGTTTTAGTGCAAATAGGGTAAGTCTTGTTTACATCATATCGGTTTCATTTTGGTAC
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| − | ACGAATGGAGAAGAAATGAAAGAGATCGAAAAAAGGAAGAGCTCGCTGTGTATCTGTCTCGTAACAGCCC
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| − | CGGTGTTACACGTGCTCTAAGAGAGATTAATTAAATCGATAAGCTACCAGAGGTTTAGTTTTCCACGTGT
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| − | TAATTAGATTGGAAAGCGAGAGAAATTAAAAATAGCGAGTAAAAATAGAGATAACCTTATTGCTATTTTG
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| − | TTTTTTTTCCAGCAACAAACTTATCTTTCAGGCTAGTTTAGGCGATCGCTTAGATTCCGCATCGTCCTTT
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| − | TCACTATTTTTTTTCTGTCAGTGACAATGTGAAAATTTATTGGACAGACGACTAGCTTGTGGTACTAGCT
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| − | AGGAAATTCCCTATCCTCGATATGAACAACTTACTCAACTCAGTAGAGTAGCAAATGCCCAAGAAAGCCC
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| − | GAGTCAATCTATTTGGAAATCCAATCTATTTTCTCGTATTCGTGTGGGAAATCAAGCTATACTAGTTGAA
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| − | ATTCACCGGAAGAAATGCACGCACTTCAATATACCAAAATTGCAAAGGAGAATCATTCGATTAACAGTGG
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| − | AATTCAACCAAGAAATGAAAAGGTATATATAGGAAATGCACTCCAACCACCAACCAATAAGTGATTCCGG
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| − | GCAATCAATTCTATCCGCGAGTTGTGGGTCTGTTCAGATTCATTATATTAGAACGCGTCACGTAATGGAT
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| − | GGAGTATTATACAACACCATTGGTTTTGCCACTAGTGTTAACTCTAATACATGGGGGTTAGTTTTACCTT
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| − | TAAACTTGGTCTAAAAGGATGGACATATGGCAATGCAATTGCATGGGGGTCATTGATTCGACCATCATGT
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| − | CTGTCCAGTGGCAACCCCCTCCCTCATCCCCTGTGGTGGGCCCCCCACGGCGCTCGTCTTCTCCCCTGTT
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| − | ACAAATACCCCACCCTCCTGCCCAGACAGCTCGCCCTGCACACACACACACACTCACACTCACACACGCT
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| − | CTCAACTCACTCCCGCTCAACACAGCGCTCACTTCTCATCTCCAATCTCATGGTGGCCGAGCACCCCACG
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| − | CCACCACAGCCGCACCAACCACCGCCCATGGACTCCACCGCCGGCTCTGGCATTGCCGCCCCGGCGGCGG
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| − | CGGCGGTGTGCGACCTGAGGATGGAGCCCAAGATCCCGGAGCCATTCGTGTGGCCGAACGGCGACGCGAG
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| − | GCCGGCGTCGGCGGCGGAGCTGGACATGCCCGTGGTCGACGTGGGCGTGCTCCGCGACGGCGACGCCGAG
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| − | GGGCTGCGCCGCGCCGCGGCGCAGGTGGCCGCCGCGTGCGCCACGCACGGGTTCTTCCAGGTGTCCGAGC
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| − | ACGGCGTCGACGCCGCTCTGGCGCGCGCCGCGCTCGACGGCGCCAGCGACTTCTTCCGCCTCCCGCTCGC
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| − | CGAGAAGCGCCGCGCGCGCCGCGTCCCGGGCACCGTGTCCGGCTACACCAGCGCCCACGCCGACCGCTTC
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| − | GCCTCCAAGCTCCCATGGAAGGAGACCCTCTCCTTCGGCTTCCACGACCGCGCCGCCGCCCCCGTCGTCG
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| − | CCGACTACTTCTCCAGCACCCTCGGCCCCGACTTCGCGCCAATGGGGTAATTAAAACGATGGTGGACGAC
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| − | ATTGCATTTCAAATTCAAAACAAATTCAAAACACACCGACCGAGATTATGCTGAATTCAAACGCGTTTGT
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| − | GCGCGCAGGAGGGTGTACCAGAAGTACTGCGAGGAGATGAAGGAGCTGTCGCTGACGATCATGGAACTCC
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| − | TGGAGCTGAGCCTGGGCGTGGAGCGAGGCTACTACAGGGAGTTCTTCGCGGACAGCAGCTCAATCATGCG
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| − | GTGCAACTACTACCCGCCATGCCCGGAGCCGGAGCGGACGCTCGGCACGGGCCCGCACTGCGACCCCACC
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| − | GCCCTCACCATCCTCCTCCAGGACGACGTCGGCGGCCTCGAGGTCCTCGTCGACGGCGAATGGCGCCCCG
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| − | TCAGCCCCGTCCCCGGCGCCATGGTCATCAACATCGGCGACACCTTCATGGTAAACCATCTCCTATTCTC
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| − | CTCTCCTCTGTTCTCCTCTGCTTCGAAGCAACAGAACAAGTAATTCAAGCTTTTTTTTCTCTCTCGCGCG
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| − | AAATTGACGAGAAAAATAAGATCGTGGTAGGGGCGGGGCTTTCAGCTGAAAGCGGGAAGAAACCGACCTG
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| − | ACGTGATTTCTCTGTTCCAATCACAAACAATGGAATGCCCCACTCCTCCATGTGTTATGATTTATCTCAC
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| − | ATCTTATAGTTAATAGGAGTAAGTAACAAGCTACTTTTTTCATATTATAGTTCGTTTGATTTTTTTTTTT
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| − | TAAAGTTTTTTTAGTTTTATCCAAATTTATTGAAAAACTTAGCAACGTTTATAATACCAAATTAGTCTCA
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| − | TTTAGTTTAATATTGTATATATTTTGATAATATATTTATGTTATATTAAAAATATTACTATATTTTTCTA
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| − | TAAACATTATTAAAAGCCATTTATAATATAAAATGGAAGGAGTAATTAATATGGATCTCCCCCGACATGA
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| − | GAATATTTTCCGATGGTGTGACGACGCCATGTAAGCTTCGGTGGGCCTGGACGGCCAGAGGTGCCAACAG
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| − | CCACGTCCAACAACCCCTGGGTCCCCCCCTAACACTCCAAACAGTAGTGAGTAGTGTCTCGTCGCGTTTT
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| − | AGTATTTGATGACAAACAAAGTGTGAGTTGAGTTAGCCACCACCAACTTGCACACGAGCACATACATTTG
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| − | TGTCCATTCTCGCCAGTCACTTCCATCTCTAGTCCTAACTCCTATCTAGCGATGTAAGCGGATAATTTCA
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| − | TCATCCGTATATAAACCTGTTTGTTATAGTTAATTTCCTATATAATACTATAACAGTATACATTTTAAAA
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| − | GAAAACAAAATTAGGATAAACAGGCCCTGCTCCTATCCATCCATGGCACTTGGAAGGACCAGACTCGGTC
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| − | ATGCCATGCCAAGCCAAGATATGGGTTATGGAAGAGTAGAGAAGAGGAGAGATGAGAGATAAGCATGCGT
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| − | TCTCCTCCTCGTTGGATGTGTATTTTGGAGGGATTTGTGTAGTAGTAGCAGCGGCGCCGCGGGGACGGAT
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| − | GCGGATGGTGGCGCTTTCGGTGGCGTTTTCCCGGGGGGGTTTTGGTTTGGCGCTTGGGGGGGATGGCATG
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| − | GCGCGGCGTGCGGCTGCACGCCACACACACGCGCGCGCACGCACGTACGTCGTCGTCGCCGCGGGCGGAC
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| − | GGTAGCTTAGGGTGGTGTGTTCCGCGCGCGGGCGCGGATTGTTCCATGCCGATCGATTTGGCGCCACCCT
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| − | CGCCGCGGCTCTTGTCGCGTCGTGCGCCTCTCTCGCGCGGTTTGTCCTTGTCGCGTTGCTCAGCCGGCGA
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| − | CGGGGGCACGGACATTGGCGATGTAGCCCTGCACGTGTCGGCCTCTCCGTTGATGAATGATGATGTATGT
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| − | ATGTATTTTTTTTTGTCTGAAGGAATTTGTGGGGAATTGTTGTGTGTGCAGGCGCTGTCGAACGGGAGGT
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| − | ATAAGAGCTGCCTGCACAGGGCGGTGGTGAACCAGCGGCGGGAGCGGCGGTCGCTGGCGTTCTTCCTGTG
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| − | CCCGCGGGAGGACAGGGTGGTGCGGCCGCCGCCGAGCGCCGCCACGCCGCAGCACTACCCGGACTTCACC
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| − | TGGGCCGACCTCATGCGCTTCACGCAGCGCCACTACCGCGCCGACACCCGCACGCTCGACGCCTTCACGC
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| − | GCTGGCTCGCGCCGCCGGCCGCCGACGCCGCCGCGACGGCGCAGGTCGAGGCGGCCAGCTGATCGCCGAA
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| − | CGGAACGAAACGGAACGAACAGAAGCCGATTTTTGGCGGGGCCCACGCCCACGTGAGGCCCCACGTGGAC
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| − | AGTGGGCCCGGGCGGAGGTGGCACCCACGTGGACCGCGGGCCCCGCGCCGCCTTCCAATTTTGGACCCTA
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| − | CCGCTGTACATATTCATATATTGCAAGAAGAAGCAAAACGTACGTGTGGGTTGGGTTGGGCTTCTCTCTA
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| − | TTACTAAAAAAAATATAATGGAACGACGGATGAATGGATGCTTATTTATTTATCTAAATTGAATTCGAAT
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| − | TCGGCTCATGGATTTCGCGAATGTGGATGGTGGATGCCCGCCTCGATGAATCCGCTTTGTCCGATAGAGA
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| − | AATTTGAATTTAAATCCGGGACCTGGATTTTGCAATGTGGACGGGTGTGCTTTGCGAAATCTGCTTTGTT
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| − | CGATAGCGCTGCACAAAACATGCGGTGGGCCCTGCATGAGAATCCGCTTCTTCTTTGTTGCCTTGGTAGG
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| − | CGAAATCGTATATGGTCCCAACGATTTTCTTTGTTTGGTTTCAACATAAATGGGAGTTTTTATGAATTTA
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| − | GGCTTATCTACATCAGAGCTACTCCTAACTTGTGATATGATGAACCAATCGTGTTCTTCTCATACTTGTT
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| − | TAAGTTGGCCAATATAGGATTAATGCAGAGTATCCAAGGGTTTTAAGATGGATCTAGTTAAGATTTGGAG
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| − | AACATAATCTACAATCATCAGCAACACTAATTATAACTAAATCAACTTGCCTTTTGAGTTCTCCGCAAAT
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| − | ATCAGAACGCTTTTTTCTTTTTTTTTCTTTTTTTTTTTTGAGGAGGTGGGGAGCACAAAATCGGAGTGAA
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| − | ATTCGGGATTCCCATTCAACCACTTCCAACCATGCCAAATCCCGGATGGTTTTTGTTTCTTGGCACTAAG
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| − | TGATGGGTCACGTTTTCACAGTAGTTGATAACTTGCAACTTGCAATCACTCTATCTTCAGCTGCTCCACT
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| − | GGATTCAACGTCCGTAGGAGCAGTAACTTGTCACAATGCTGAGCAGAAAATAACCGCTAGGAATATCAAA
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| − | TTGCACAAAATTATAATGTCACTGTTGAGTGATGAGCAGTAACTCATGAAAGAATCCAAAGTTCCCATGA
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| − | GTTCCAGAATGTTTGACTGATATGACAGACAAACTTTTGTAAGGTTCTCCTAAATAACACAAAGAAATTT
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| − | CTCGTACACTACATGGCTGTATGGATTGAACATAATTTGCCTGTGACACTTGTCACACTTGTGACACTTC
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| − | CAGGTTCCAATACAACCTTACAGCAATGGAGCAAGAGCACAGCAAGAAGAGAATATAGAGTGCATTTGTT
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| − | TGGCAAGAAAGAAAAAAAATGATTCTACAATTACATCAAGGCTTATGCCTTTTCAGAATCAACAAGAATA
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| − | GTATGCATAT
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| | 基因(座)名称 半矮秆基因 dee-geo-woo-gen dwarf | | 基因(座)名称 半矮秆基因 dee-geo-woo-gen dwarf |
| | 基因符号 sd1; GA20ox-2 | | 基因符号 sd1; GA20ox-2 |
| Line 105: |
Line 23: |
| | Monna等的研究报道表明：日本晴、Sasanishiki和Calrose等正常野生型水稻的3个外显子大小分别是558、318和291bp，2个内含子分别为105和1471bp；DGWG型半矮秆种IR24、Habataki和Milyang 23从外显子1中部起有383bp的缺失，包括外显子1和2的278bp序列及105bp的内含子；Calrose76为外显子2中编码第265位氨基酸的CTC突变为TTC，导致Leu（亮氨酸）突变为Phe（苯丙氨酸）。 | | Monna等的研究报道表明：日本晴、Sasanishiki和Calrose等正常野生型水稻的3个外显子大小分别是558、318和291bp，2个内含子分别为105和1471bp；DGWG型半矮秆种IR24、Habataki和Milyang 23从外显子1中部起有383bp的缺失，包括外显子1和2的278bp序列及105bp的内含子；Calrose76为外显子2中编码第265位氨基酸的CTC突变为TTC，导致Leu（亮氨酸）突变为Phe（苯丙氨酸）。 |
| | Sasaki等报道认为先前发现的编码GA20氧化酶基因(GA20ox-1)与sd-1无关，而他们新发现的GA20ox-2与sd-1紧密连锁。比较了四个矮秆品种与野生型在sd-1位点上的序列差异，发现野生型水稻sd1等位基因的3个外显子大小分别为557、321和291bp，2个内含子分别为103和1472bp(右图)；矮秆品种Deo-geo-woo-gen及其衍生种在该位点缺失383bp，包含103bp的内含子1；矮秆品种Jikkoku中编码第94氨基酸的GGG变为GTG，导致甘氨酸变为缬氨酸；矮秆品种"Calrose 76"中编码第266氨基酸的CTC突变为TTC，导致Leu（亮氨酸）突变为Phe（苯丙氨酸）；矮秆品种Remei中编码第349氨基酸的GAC变为CAC，导致天冬氨酸变为组氨酸。GA20ox-2在叶片、茎秆、未开放的花中表达强烈，而GA20ox-1基因则是在未开放的花中表达，揭示了sd-1水稻株高降低，而产量不受影响的原因。 | | Sasaki等报道认为先前发现的编码GA20氧化酶基因(GA20ox-1)与sd-1无关，而他们新发现的GA20ox-2与sd-1紧密连锁。比较了四个矮秆品种与野生型在sd-1位点上的序列差异，发现野生型水稻sd1等位基因的3个外显子大小分别为557、321和291bp，2个内含子分别为103和1472bp(右图)；矮秆品种Deo-geo-woo-gen及其衍生种在该位点缺失383bp，包含103bp的内含子1；矮秆品种Jikkoku中编码第94氨基酸的GGG变为GTG，导致甘氨酸变为缬氨酸；矮秆品种"Calrose 76"中编码第266氨基酸的CTC突变为TTC，导致Leu（亮氨酸）突变为Phe（苯丙氨酸）；矮秆品种Remei中编码第349氨基酸的GAC变为CAC，导致天冬氨酸变为组氨酸。GA20ox-2在叶片、茎秆、未开放的花中表达强烈，而GA20ox-1基因则是在未开放的花中表达，揭示了sd-1水稻株高降低，而产量不受影响的原因。 |
| − | Spielmeyer等的报道认为野生型水稻sd1等位基因的3个外显子大小分别为557、322和291bp；DGWG型籼稻半矮秆品种Doongar在GA20ox2编码区（外显子1和2）缺失了280bp；矮秆品种"Calrose 76"中亦是编码第266氨基酸的CTC突变为TTC，导致Leu（亮氨酸）突变为Phe（苯丙氨酸）。·参考文献 | + | Spielmeyer等的报道认为野生型水稻sd1等位基因的3个外显子大小分别为557、322和291bp；DGWG型籼稻半矮秆品种Doongar在GA20ox2编码区（外显子1和2）缺失了280bp；矮秆品种"Calrose 76"中亦是编码第266氨基酸的CTC突变为TTC，导致Leu（亮氨酸）突变为Phe（苯丙氨酸）。 |
| − | 1. Wolfgang Spielmeyer;Marc H. Ellis;and Peter M. Chandler
| + | 【相关登录号】 |
| − | Semidwarf (sd-1), "green revolution" rice, contains a defective gibberellin 20-oxidase gene
| + | NCBI GeneBank：AB077025, AF465255, AF465256, AY114310, U50333 |
| − | Proceedings of the National Academy of Sciences, 2002, 99(13): 9043-9048
| + | RAP-DB：Os01g0883800 |
| − | 2. Lisa Monna; Noriyuki Kitazawa; Rika Yoshino; Junko Suzuki; Haruka Masuda; Yumiko Maehara; Masao Tanji; Mizuho Sato; Shinobu Nasu and Yuzo Minobe
| + | MSU：LOC_Os01g66100 |
| − | Positional Cloning of Rice Semidwarfing Gene, sd-1: Rice "Green Revolution Gene" Encodes a Mutant Enzyme Involved in Gibberellin Synthesis
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| − | DNA Research, 2002, 9(1): 11-17
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| − | 3. A. Sasaki; M. Ashikari;M. Ueguchi-Tanaka; H. Itoh;A. Nishimura; D. Swapan; K. Ishiyama;T. Saito; M. Kobayashi; G. S. KhushH. Kitano||; M. Matsuoka*
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| − | A mutant gibberellin-synthesis gene in rice
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| − | Nature, 2002, 416(): 701-702
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