Difference between revisions of "Os10g0536100"
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=== Knowledge Extension === | === Knowledge Extension === | ||
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| − | + | To confirm the physical interaction between OsMADS50 and OsMADS56, we studied their in vivo interaction by conducting Co-IP experiments. Myc-tagged OsMADS50 was first co-transfected with HA-tagged OsMADS56 into rice protoplasts before whole-protoplast extracts expressing epitope-tagged proteins were immunoprecipitated with anti-Myc antibodies. Immunoblot analysis with anti-HA antibodies revealed that HA-tagged OsMADS56 was co-precipitated with the Myc-tagged OsMADS50 (Fig. 8b). Similarly, HA-tagged OsMADS50 was co-precipitated with the Myc-tagged OsMADS56 (Fig. 8b). However, neither OsMADS50-HA nor OsMADS56-Myc alone showed such co-precipitation.These experiments supported our findings from the yeast two-hybrid trials that OsMADS50 and OsMADS56 do interact with each other. We also showed that OsMADS50 and OsMADS56 form homodimers, based on those yeast-two hybrid (data not shown) and Co-IP experiments (Fig. 8c). | |
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 03:30, 10 June 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
OsMADS50 and OsMADS56 , which is highly homologous to Arabidopsis SOC1, are affected by the circadian clock.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a. OsMADS50 is a flowering activator that functions only under LD conditions. But OsMADS50 and OsMADS56 function antagonistically in regulating LD-dependent flowering by controlling expression of OsLFL1 and Ehd1. So OsMADS56 is an LD-specific floral repressor.OsMADS50 and OsMADS56 form a complex that regulates downstream target genes in order to regulate the Rice's flowering time. OsMADS56 is highly homologous to OsMADS50. OsMADS56 is located on chromosome 10, and consists of eight exons that encode a typical MIKC-type MADS-box protein (Fig. 2a). When their full-length protein sequences are aligned, this MADS-box protein shares 60.8% homology with OsMADS50. This implies that OsMADS56 may also regulate flowering time.
Expression
(1)Using the maize Ubiquitin promoter in transgenic plants that over-expressed OsMADS56 (56OX; Fig. 2a). Among 17 primary (T1) plants, 9 flowered late by 1–2 weeks in the paddy field. RT-PCR analyses of three normal plants (#4, 19 and 20) and five late-flowering plants (#6, 8, 10, 11 and 13) demonstrated that the extent of late flowering was correlated with OsMADS56 transcript levels (data not shown). Then selected Lines 8 and 10 for further analyses of the next generations when grown in controlled environments. Both lines displayed a dominant late-flowering phenotype under LDs. Homozygous progeny flowered late by 3–4 weeks under LD (Fig. 2b,c), although no significant change was observed under SD (Fig. 2d). These results suggest that OsMADS56 is an LD-specific floral repressor. They observed no other phenotypic changes in this overexpressor except for the delayed-flowering phenotypes.
(2)Real-time RT-PCR analyses indicates that OsMADS56 transcript levels were low at the seedling stage but gradually increased to a maximum at 4 weeks after germination as the observation made by CHOONG-HWAN RYU1 etc. (fig.3b) (3)OsMADS56 and OsMADS50 control the Rice's flowering time through regulating their communal downstream genes OsLFL1-Ehd1 .The expression patterns of Ehd1, Hd3a RFT1 and OsLFL1 reveal it.(fig3c-f) (4)The mRNA levels of OsMADS50 and OsMADS56 along with other flowering regulators such as Ehd1, Hd1 reveals that OsMADS50 and OsMADS56 also have daily rhythms.(fig9) The transcript levels of them is similar to that of Ehd1. (5)OsMADS56 is located on chromosome 10, and consists of eight exons that encode a typical MIKC-type MADS-box protein.
Evolution
Please input evolution information here. Although the osmads50 null mutant plants flowered late, they did eventually bloom after 2 months. This indicates that other positive regulators induced flowering in the absence of OsMADS50.OsDof12 functions independently from OsMADS50 as a positive regulator of Hd3a under LD-specific conditions.Unlike the osmads50 mutants, suppression of OsID1 causes extremely late flowering under LDs.
Knowledge Extension
To confirm the physical interaction between OsMADS50 and OsMADS56, we studied their in vivo interaction by conducting Co-IP experiments. Myc-tagged OsMADS50 was first co-transfected with HA-tagged OsMADS56 into rice protoplasts before whole-protoplast extracts expressing epitope-tagged proteins were immunoprecipitated with anti-Myc antibodies. Immunoblot analysis with anti-HA antibodies revealed that HA-tagged OsMADS56 was co-precipitated with the Myc-tagged OsMADS50 (Fig. 8b). Similarly, HA-tagged OsMADS50 was co-precipitated with the Myc-tagged OsMADS56 (Fig. 8b). However, neither OsMADS50-HA nor OsMADS56-Myc alone showed such co-precipitation.These experiments supported our findings from the yeast two-hybrid trials that OsMADS50 and OsMADS56 do interact with each other. We also showed that OsMADS50 and OsMADS56 form homodimers, based on those yeast-two hybrid (data not shown) and Co-IP experiments (Fig. 8c).
Labs working on this gene
1.CHOONG-HWAN RYU1 ,Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea
2.SOON JU PARK2&CHANG-DEOK HAN, Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 660-701, Korea
3.Nam J,Institute of Molecular Evolutionary Genetics and Department of Biology, Pennsylvania State University University Park, PA 16802, USA
4.Lee S, National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea
5.Kaufmann, Melzer & Theissen , Friedrich-Schiller-Universität Jena, Lehrstuhl für Genetik, Philosophenweg 12, D-07743 Jena, Germany.
6.Takada S. & Goto K,Research Institute for Biological Sciences, Okayama 716-1241, Japan
References
1. Choong-Hwan Ryu;Shinyoung Lee;Lae-Hyeon Cho;Song Lim Kim;Yang-Seok Lee;Sang Chul Choi;Hee Joong Jeong;Jakyung Yi;Soon Ju Park;Chang-Deok Han;Gynheung An OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)-dependent flowering in rice Plant, Cell & Environment, 2009, 32(10): 1412-1427
2. Jongmin Nam;Kerstin Kaufmann;Günter Theißen;Masatoshi Nei A simple method for predicting the functional differentiation of duplicate genes and its application to MIKC-type MADS-box genes Nucleic Acids Research, 2005, 33(2): e12
3.Peng L.T., Shi Z.Y., Li L., Shen G.Z. & Zhang J.L. (2007) Ectopic expression of OsLFL1 in rice represses Ehd1 by binding on its promoter. Biochemical & Biophysical Research Communications 360, 251–256.
4.Arora R., Agarwal P., Ray S., Singh A.K., Singh V.P., Tyagi A.K. & Kappor S. (2007) MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress. BMC Genomics 8, 242.
5.Lee S., Kim J., Han J.J., Han M.J. & An G. (2004) Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO1/AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog in rice. ThePlant Journal 38, 754–764.
Structured Information
| Gene Name |
Os10g0536100 |
|---|---|
| Description |
Transcription factor MADS56 |
| Version |
NM_001071703.1 GI:115483149 GeneID:4349237 |
| Length |
10441 bp |
| Definition |
Oryza sativa Japonica Group Os10g0536100, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:21318476..21328916 |
| Sequence Coding Region |
21318861..21319045,21325700..21325778,21327505..21327566,21327666..21327765,21327903..21327944 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:21318476..21328916 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:21318476..21328916 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggtgcgggggaggacggagctgaagcggattgagaacccgacgagccggcaggtgaccttctccaagcgccggaatggcctcctcaagaaggcgttcgagctctccgtcctctgcgacgccgaggtcgccctcatcgtcttctccccccgcggccgcctctacgagttcgccagcgcccccagcctacagaaaaccatcgaccgctataaagcatacacaaaggatcatgtcaacaataagacaattcaacaagatatccagcaagtcaaagatgatactttaggcttggccaagaaacttgaagctcttgatgagtccagacggaaaatattgggagaaaatttagaaggattctctattgaagaactgcgtggtctagaaatgaaacttgagaagagcctccacaagataagactaaagaagaccgagcttctggagcagcagatagccaagctgaaagagaaggagcggactttgcttaaagacaacgaaaatttacgcggaaagcatcgcaaccttgaggctgcggcgctggtggctaaccacatgacgacgacgacggcgccggcggcgtggccgcgggacgtgcctatgacgagcagcacagccggcgccatggacgtggagactgatctgtacattggattgcccggcactgagcgctcctccaaccggtcggagacaggttga</cdnaseq> |
| Protein Sequence |
<aaseq>MVRGRTELKRIENPTSRQVTFSKRRNGLLKKAFELSVLCDAEVA LIVFSPRGRLYEFASAPSLQKTIDRYKAYTKDHVNNKTIQQDIQQVKDDTLGLAKKLE ALDESRRKILGENLEGFSIEELRGLEMKLEKSLHKIRLKKTELLEQQIAKLKEKERTL LKDNENLRGKHRNLEAAALVANHMTTTTAPAAWPRDVPMTSSTAGAMDVETDLYIGLP GTERSSNRSETG</aaseq> |
| Gene Sequence |
<dnaseqindica>386..570#7225..7303#9030..9091#9191..9290#9428..9469#9585..9626#9939..10042#10052..10130#cgccctcgtctcgtctcgtctctccactcccccacctcccatccgctactgctagtactactactacccggcctctcgccttctcgcgcggccgcgatttccgttcgcaaatccgcacccacggcggcgcgaggtcgccgtcgtcgtcgagccatttcgggtgagagggggggtctccggtttttctcagatctgtccacttttgtccagttttgatctggtgatttaatatactatttttcacttctggtggtttgcggcgacgcgtgtgcaggggggtagtttataagggcttgcgcgaggagggggaggagtagcgggtagcggcggctcgagtcgagtcgtcgtcgtcgtcggcggcggcggtcgagagagatcgggatcgatggtgcgggggaggacggagctgaagcggattgagaacccgacgagccggcaggtgaccttctccaagcgccggaatggcctcctcaagaaggcgttcgagctctccgtcctctgcgacgccgaggtcgccctcatcgtcttctccccccgcggccgcctctacgagttcgccagcgcccccaggtataccttcttctcgttctcggcaagttctcgccggaaattattctctcgctcgtttcgccctcgtcgtgctgctgcatcaaatgcaaatgcgatttcattctctctctctctctctgtggtaatcgggtgatttttgtggtttggggggtttgaggattcggctgttgggattgcttgttcgtcagagtttgaattcctgcccttaattccgtcgaacaaaaatgggttctctgttcggatttggcacctgcggatttggccaccgctgctgcgacgacgaagacgacggcgacggcgaccgccggccacacacactactagtagcctactagttcgtcttgcttctcacttgctattattgttattattggaagctgtgaactcctgtagaagttgaccccttctctccccctaaatatctgtacggccctggagaccggatggatatgcccacacctcagcactctctcacagtcaccaccagttgcctgtagtatctcttcttcattcatcttcttcttcctctcacatgttaacaactgttccatggagcatgtccacgagctctggattgattctctggcgcgtctctgtgcatcttccttccccattcgtcgattcttgttctcgatccgcgggaaaattgacgattaaaggaggctcctgacctctgaataaaatcaaggacaaaaacgtacaagaaggcagaggtaataaacccaagatagccaatatacaaagaaaaaccaaagtaaatggcagccaatgggaggaaggaggccggccggcgtgcgtggccagtaattgcggggtaggaagaaggctgcgagcctgcgagcgtgcgagactacaaatggagaaacaagctaatgtgacatccggtcagtgagatgagagatcattgggcgcctacctgcaccctctctctctctctctcgatccacaccactgcactgctcccctctcgatcgtacactggctgatgagctagccaagttgatttctctcgcaaaacgtaccacggattggtcgcatcagctccgttcgtcgagggtatggtgtgggggtatctttccatcatacacagtatgacgttggccacagtacatagcatgcgatatggctgtttctgaatattttttcagttttcagcaggtccgaatttgttaggtggaaccgtgatttatatatttctcccctcttccttgtggatatagtactcgtattgtgtatcttactcctagttctcttgcacccactccagctggagagtggagagagtggagagtacgtaccttatctggcgcacactgtactataatccttatattttggtgatcaggtgcaccgtttattatagattaatcatcatctcttagacatggcttgttgcaccttaatatccatctctttatcaggcttatatgtctacggcagtgaaaaagcgttgccagttaattcattgtcgtgcatgtagtttccaaaccatagtctagggaacactcaatgttgttccatgatattaagtgatgttaagtactccataacttaattagtactccatgccaatactcgacaacataatatttccatcacacttcatatatatgtatgctctccctctttctcaattaggaagatctatctagaaaattcttgagtttgcaagctatgatatgcaacgaactttcttctaatgtaagtgcaaaactatggattgtcattcattacttacgttacttcttaataaacattatcaagaagctagattgtagagcatgtaaagctgagtggaaaaggagttttggtaagcatgtgattgacatgtcttataggtcgtcgattgatctgcccaaacaacagtacaaatgaagttaattctactgtgaatagcatgtgataactcgcaatccgtagaatgctttggctttaaaacaataattttaagagtgtgagcaatgcttatgttacttcatcagattatcgtgtgccttaattcttatctgttgatcatatttagtactcctcctacccataaatatatgaacacttgaagcatttaaaatttatccacaaatatgatgattattcctagagtactactgatccctcttcccaaaataagtgaccaatttggatgtaaaatttgtttcaagataagtgatcacattacagtgctacttgtctattaatcacctcttgttcaaacttcttatcttacctcaactaccctcccacttctaaacatccctcatttaggtcatttgcctttcctcattatttggctcatatataagtgataaagtagcttattcatttattttagaacagatcgatggagtacttattacaacaatcacccgtcattcaaatttctttctaacttacactcaaccaccgtcccacctccatctctctatcttctcctccaactctctctcttttaatgaggggcaccaaaatattttcttctcaactttaatctctactaaacaatttggcatgtgtatatttgttaatggagggagtattttctaagttctaagtacttgatatatatcgtttaaccagaaatctatacctatgtgaaaaaaatacattgtccatagatcgatctttcatgagtatattactgaatcatgccaaatttggccaatgttacatattaattatgatcttaaagtttgttctgaaattactttgccttgaatcttccaagcaagtatatatatacgtgtaggcaccacacttctcatggtcaaatttagatatttatcaacctaattagtcagtccatatatagagagttaatgcaactagctagttgctttgtctaatgtgagatcttttgtacgtgaactgtaaacattatatatacaacctccatcctatattacttgtcgctttgagtttttatttgtaatgtttgatcattcgtcttattcaaaaattttttacaattgttatttattttatttgtgatttgctttattatcaaaagtattttaaatatgacttatcttttttatatttgcactaatttttcaaataaaacaaagggtcaaacgttgtaacgaaaaagtcaaagcgacatctattatgagacggaggtagtatgatcggtgtgctgtctttagtcctgtttaagatacttaatttctctgaaatacaattgctcataattacagtgttgttgtcagttttgtcttcttttccaaatactttatttgaaatatggataaatcaaacttaccttttaccctaataaactacactatacatactaatcttaataacatttatgcataacatttatggtttttgttcattcttttcaaatccgttccattctgggcagaatggggcaatgcaagtactgtcacaaagaacaaagaacagatggtgcagccggatatgcatatatatataccagctgaatttttggccgtttcaggaaaacttcaatttaaaatcaatgttggtactatcaaattaatagctgcaaagtctccactaggtctagcaagaaagtagcagctcaatggtagtaataacttcatatgtttatcgaacagatattagctttctttaaaagtccattaccaaacaaggcttatctaggggcatagtacccccatagacccatatgtaggagtaacaaagagaaaatgattaattacaaatattttaatgggcttgtttggcagggctccaactctaagtactagctaaattctgttctacctctctagttcattttataaaagcactctagcattctcctctctctttgtaggtggagctgaaaccgtttggttgggctctagctcaaggagaggtggagttggagctttgccgtatgggcccaatgtatataaaaaatatctataatttggttatagagtatttgtatttcaatgggaaaaaggtatatgtgttaatagtattatactcttctcataaaaatgagtgtgaaactaacttttgtaataatccttactattagttagctgagctcgaactattctttagtgtacaggcaacacgccccaattctacaagtgctgcaaccaaagctttcaatactggttttgccggaaatacccgtaccgcttgctggttgttacttcttccgtttcataatgtaagtcattctaacattgcccacattcatacaaatgttaatgaatctagacataaacatatgactagattcattaacatctatatgaatgtgggcaatgctagaatgacttacgttatgaaatggagggagtattagacaaatgttcaaatctaaaattttgaactcactgtggttcag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ccattaattatcttgagaaaatgtgcaattgatctctgcagcatcgcaaccttgaggctgcggcgctggtggctaaccacatgacgacgacgacggcgccggcggcgtggccgcgggacgtgcctatgacgagcagcacagccggcgccgccgacgccatggacgtggagactgatctgtacattggattgcccggcactgagcgctcctccaaccggtcggagacaggttgaagaaggccagccacagcaacagctgctatatagcatgcagtagcagcaacgacgccagtgacatcctgttcatctcacaagaataaccagagctctacgcatgcgcacatgaaacccaggcatgcagttctcggtgggtaaattattatctcgcattattagctatatgcgtgtccttgtctttttctttaggcaatggaataaaatttccggtaagtacaaacacaaacggaaacaaagaattaacaaagctgtagcagaccagatcggcaaattgaaatgccaggtaatccagtatatatttcgcttcc</dnaseqindica> 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| External Link(s) |
