Os01g0749300

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Annotated Information

Function

Fig.01 Identification of Amino Acid Residues in the DBD of Ta HsfA4a That Are Critical for Cd Tolerance

Fig.02 Enhanced Cd Resistance in Ta HsfA4a–Overexpressing Rice Plants

Fig.03 Plant Proteins with Homology to Ta HsfA4a

Os HsfA4a is a rice ortholog of Ta HsfA4a which plays a role as Cd tolerance gene of Wheat. Ta HsfA4a is the most similar to the class A4 Hsfs that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a(HsfA4a). Cd tolerance is enhanced in rice plants expressing Ta HsfA4a and decreased in rice plant with knocked-down expression of Os HsfA4a. The DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Os HsfA4a confers Cd tolerance in the root of rice by upregulating metallothionein(MT) gene expression in planta. HsfA4a is unique not only in the high level of tolerance that it confers, but also in its Cd specificity. Os HsfA4a greatly enhances Cd tolerance in rice, but does not significantly enhance their tolerance to the other heavy metals, namely, lead, zinc, cobalt, manganese, silver, mercury, and iron.

Expression

The DBD binding domain of Os HsfA4a is critical for Cd tolerance. The region for the direct binding to DNA is conserved. However, a few animo acids outside the direct DNA binding region are different in Os HsfA4a when compared with other Hsfs. Ala-31 and Leu-42 in the DBD domain of Ta HsfA4a are critical for its ability to confer Cd tolerance. As a transcription factor, HsfA4a is expected to function by regulating the expression levels of other genes. CUP1 contributes to HsfA4a-induced Cd tolerance by acting as a downstream target of HsfA4a. Overexpression of HsfA4a enhances Cd tolerance in rice. The small, Cys-rich metallothionein proteins are the main chelators of Cd and other heavy metals in animals. HsfA4a confers Cd resistence in rice via upregulation of metallothionein, at least in part. MT genes are important targets of HsfA4a and MT expression is necessary for Cd tolerance in plants. HsfA4a is upregulated by Cd treatment and it in turn upregulates the expression of target genes, including MTs, and thereby confers Cd tolerance in planta. The site where HsfA4a confers Cd tolerance is the root and not the shoot. HsfA4a most likely activates multiple Cd tolerance mechanisms, similarly to the mechanisms described for MTF-1 in animal cells. The Cd-responsive induction of the Os HsfA4a genes is rapid and strong in rice root, and their expression induced a well-known Cd tolerance gene, metallothionein, leading to Cd tolerance in the plants.

Evolution

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Labs working on this gene

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References

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Structured Information

Gene Name	Os01g0749300
Description	Similar to Heat shock factor
Version	NM_001050782.1 GI:115439934 GeneID:4325556
Length	2317 bp
Definition	Oryza sativa Japonica Group Os01g0749300, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 1
Location	Chromosome 1:33126259..33128575
Sequence Coding Region	33126439..33126651,33127234..33128343
Expression	GEO Profiles:Os01g0749300
Genome Context	<gbrowseImage1> name=NC_008394:33126259..33128575 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008394:33126259..33128575 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctga</cdnaseq>
Protein Sequence	<aaseq>MEGGGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQ PEFCRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRR KPIFSHSSHSQGAGPLTDNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQA LEEKLFVVEDQQRSLISYVREIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQ ENQIMPCDLTNSPAQTFYRESFDKMESSLNSLENFLREASEEFGNDISYDDGVPGPSS TVVLTELHSPGESDPRVSSPPTRMRTSSAGAGDSHSSRDVAESTSCAESPPIPQMHSR VDTRAKVSEIDVNSEPAVTETGPSRDQPAEEPPAVTPGANDGFWQQFLTEQPGSSDAH QEAQSERRDGGNKVDEMKSGDRQHLWWGKRNVEQITEKLGLLTSTEKT</aaseq>
Gene Sequence	<dnaseqindica>181..393#976..2085#aatccgagagggatttttcctcgcaaccaaccaaaccaaccaaacccccaaccaagcgagcaagccgccttcgccgggggggcccccaccctgacggcaaccctagccctccgatcaccggcgctgagactgatcccgccgccgtaggaggaggaggaggaggtggtggtggtggtggtgatggaggggggcggcgggggcgggtcgctgccgccgttcctgagcaagacgtacgagatggtggacgacccgtcgacggacgcggtggtggggtggacgcccgccggcaccagcttcgtcgtcgccaaccagcccgagttctgccgggatctgctccccaagtacttcaagcacaacaacttctccagcttcgtgcgacagctcaacacctatgtaagggagctcgcctcttaacctcatgtgtgcctttctgtcttcctctgctctgccgctgatgcttgaattggggcttggtcgtaatgcgcgcccgccgggacctatgggctgggtaggaatgccggatttgttagaattgggcgagtcctttgctgtaattactcctgtttaggatttattactttcagcacaaaatattttataagttttcgttgattgctagtatagcatattctgttcattttaggattttaagacagtacttgtgaataaccaaggtcaactcttgttctaccaagaaaattcaagtagcttattgcaatgttattgtcttctccatccggttgaagttgtacaaaaatgtttgataatggtgagtgaggttgaaatagatgtgtgttcacatcaatgtcaaagctccaaactgttttagaattgaagcaagctatccggtacagttcattccttgtggtggcagtgcatagttttttttagccttgatcatgtatgattttttttccccttttggctgcttgagttatgctattttaatgaggatccccttttgttctcatttagggctttaggaaagtagatccagaacaatgggaatttgcaaatgaggatttcataaaaggacagcggcaccggttgaaaaatatacatagacgcaaacctatattcagccattcatcacacagtcagggtgctggaccattaacagataatgaaaggaaagactatgaggaagaaatcgagaggctcaaatccgataatgcagcactgtcctcagagcttcaaaataatacgttgaagaaacttaatatggagaaacgaatgcaggctttggaagagaagctatttgttgtggaagatcagcagaggagtctgatatcatacgtcagagagattgtaaaggcaccaggatttctttctagctttgtacagcaacaagatcatcacaggaagaagaggagactgccaataccaatctccttccatgaggatgcaaatactcaggagaaccagatcatgccttgtgacttgaccaactcaccagctcagacattttacagagaatcatttgacaagatggaatcatccttgaactcattagaaaatttccttcgagaagcaagtgaagaatttggtaatgatatttcatatgatgatggtgtcccaggcccttcttcaactgttgttctcacagagctccattcacctggtgaaagtgatccccgtgtttcatcacctccaacaaggatgcgtacttcgtcagctggtgcaggagattcgcactcctctcgtgatgtagcagagtctaccagctgtgcagaaagtcctcctattcctcaaatgcattctcgtgtagatacacgagcgaaggtttctgaaatagatgttaattcagaacctgctgttacagaaactggtccatcaagagatcaacctgctgaagagcctcctgctgtcacacctggtgcaaacgatggcttctggcagcagtttcttaccgagcagcctggatcatcagatgcacatcaggaggctcaatcagaacggcgagatggaggaaacaaggtggatgagatgaaatcaggagatcgacaacatctttggtggggcaaaaggaacgttgagcagataacagaaaaacttggacttctcacctcaacggagaaaacctgacataattttgtttttctttttgtagatgaaaaactattaattcaggaacgctgacattggtgccttcctcagtgagatatgttgactaacatctatttcgcagtgtaccgtattttttccatcatgtaccgtaatgtatcatagtgccatacattcatctttggtccattgacactttctgatatcattgtgcgtgatttctaaaatattctcaagtttggttaaatgttac</dnaseqindica>
External Link(s)	NCBI Gene:Os01g0749300, RefSeq:Os01g0749300