Os01g0952800

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Annotated Information

Function

OsIRO2, an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcription factor, is responsible for regulation of the genes involved in Fe homeostasis in rice.[1] This gene has the activity of transcription, and is an important regulatory factor of grasses in the iron reaction, to be responsible for regulation of the key genes involved in Fe absorption. Under iron deficiency conditions, OsIRO2 is an indispensable factor for effective absorption to iron. In plants of OsIRO2 RNAi, due to the expression of OsIRO2 declining, the synthesis of mugineic acid is decreased. Meanwhile, the secretion of 2'-deoxy mugineic acid is reduced, resulting in a decrease of iron absorption, so that the iron content in the plants drops, leaves yellowing and its growth is inhibited. Under conditions of iron deficiency, unknown transcription factor binds into the promoter of iron deficiency OsIRO2 response cis-acting elements (IDE), to adjust the expression of OsIRO2, then OsIRO2 binds to the related gene or promoter sequences which is CACGTGG about iron absorption, regulating the expression of these genes, and promoting the absorption of iron in rice under iron deficiency conditions.[2]

Expression

Spatial pattern of OsIRO2 expression. During germination, OsIRO2 expression was detected in embryos(Fig.1). OsIRO2 expression in vegetative tissues was restricted almost exclusively to vascular bundles of roots(Fig.2) and leaves(Fig.3), and to the root exodermis under Fe-sufficient conditions, and expanded to all tissues of roots and leaves in responseto Fe deficiency. OsIRO2 expression was also detected inflowers and developing seeds(Fig.4). Plants overexpressing OsIRO2 grew better, and OsIRO2-repressed plants showed poor growth compared to non-transformant rice after germination.[2]