OsMYB103L

OsMYB103L encodes an R2R3-MYB transcription factor, it influences leaf rolling and mechanical strength in rice (Oryza sativa L.).

Annotated information

Function

OsMYB103L, which id also called Os08g05520, encodes an R2R3-MYB transcription factor. OsMYB103L may target CESA genes for regulation of cellulose synthesis thus influences leaf shape and mechanical strength in rice, which makes it a potential engineering target for desirable leaf mechanical properties. OsMYB103L is localized in the nucleus and has transactivation activity. Overexpression of OsMYB103L in rice could result in a rolled leaf phenotype, and significantly higher levels of several cellulose synthase genes (CESAs), and a significant cellulose content increase. Knockdown of OsMYB103L by RNA interference could lead to a decreased level of cellulose content thus reduced mechanical strength in leaves. Meanwhile, the expression levels of the relevant CESA genes could also be decreased.

Structural and Homology Features

According to the rice genome annotation database ([1]), LOC_Os08g05520 encodes a putative R2R3- MYB family transcription factor with a length of 359 amino acids and a molecular mass of approximately 40 kD. The Pfam database ([2]) shows that the deduced protein has two MYB DNA-binding domains (PF00249) at the N-terminus. As revealed by phylogenetic analysis of the related MYB transcription factors in Arabidopsis thaliana and rice, Os08g05520 is closely related to At1g63910 (AtMYB103). Protein sequence alignment showed that they are highly conserved in the predicted R2- and R3-MYB DNA-binding domains. That's why Os08g05520 was designated as OsMYB103L (Oryza sativa MYB103 Like).

Expression

To determine the subcellular localization of its expression, researchers fused green fluorescent protein (GFP) to the C-terminus of OsMYB103L to produce an OsMYB103L-GFP fusion protein and monitored the fluorescence of the transiently expressed fusion protein in both rice protoplasts and onion (Allium cepa L.) epidermis cells. The results indicated that OsMYB103L is a nuclearlocalized protein, just like most transcription factors.

After determining the expression level of OsMYB103L in various rice organs using quantitative real-time PCR (qRT-PCR), it was found out that The expression level of OsMYB103L was highest in culm, lower in root, leaf and mature panicle, and lowest in young panicle.(see the figure below) File:20140611134107.jpg