Difference between revisions of "Os06g0561000"
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| − | + | '''''OsMIOX''''', a '''myo-inositol oxygenase gene''', '''improves drought tolerance''' through '''scavenging of reactive oxygen species''' in rice<ref name="ref1"/>. | |
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | ''OsMIOX'' has a '''specific function''' in '''drought stress tolerance''' by '''decreasing oxidative damage'''. The '''expression levels''' of '''ROS scavenging genes''', ''CatB'', ''POD1'', ''POD2'', ''APX1'' and ''APX2'' were '''indeed up-regulated''', which '''may''' contribute to '''decrease the accumulation of ROS''' in OsMIOX-overexpressing plants under drought stress<ref name="ref1"/>. | |
| + | |||
| + | ===Mutation=== | ||
| + | Transgenic lines and wild type<ref name="ref1"/>: | ||
| + | *The transgenic rice lines overexpressing ''OsMIOX'' showed obviously '''improved growth performance''' in the medium containing 200 mM '''mannitol'''. | ||
| + | *Further, the '''survival rate''' of '''leaves''' from the transgenic rice lines was significantly '''higher''' than that of the wild type plants under '''polyethylene glycol treatment'''. | ||
| + | *It was discovered that the '''activity of ROS scavenging enzymes''' and '''proline content''', as well as the transcript levels of '''many ROS scavenging genes''' were significantly '''increased''' in transgenic plants compared to the wild type plants under drought stress conditions. | ||
| + | *Four-week-old transgenic homozygous lines and the WT were treated with 20% PEG6000 for 6 days and then allowed to recover for 17 days. | ||
| + | **The transgenic lines '''recovered more quickly''' than the WT, and showed a total recovery of 38–46%, while the WT plants were '''severely affected''' by drought stress and had only 18–22% recovery. | ||
| + | **The '''water loss''' was determined by comparing the '''rates''' of '''change in fresh weight''' of the '''detached leaves'''. The water loss assay showed that the transgenic lines had a '''slower dehydration rate''' compared to WT, with a 0.28–2.6% lag from the beginning to 6 h after dehydration. | ||
===Expression=== | ===Expression=== | ||
| − | + | *''OsMIOX'' was expressed '''predominantly''' in the '''roots''' and '''induced by drought''', '''H<sub>2</sub>O<sub>2</sub>''', '''salt''', '''cold''' and '''abscisic acid'''. ''OsMIOX'' was more strongly induced in IRAT109 than that in Nipponbare under all treatments except for cold. The '''expression peak''' of ''OsMIOX'' was '''later''' in IRAT109 than that in Nipponbare under all treatments except salt and cold, indicating that the '''damage in UR''' was '''weaker''' and '''occurred later''' than that in LR, which suggesting that ''OsMIOX'' may be involved in abiotic stress responses, and related to drought tolerance in plant<ref name="ref1"/>. | |
| + | |||
| + | *The '''GUS activity''' was detected in '''spikelets''', '''stem''', '''leaves''', especially in '''roots''', and the activity was '''strongly induced by drought stress''', indicating that ''OsMIOX'' was expressed in all rice organs and induced by drought stress. | ||
| + | Overexpression of ''OsMIOX'' '''increases free proline contents''' under drought stress and '''increases the ROS-scavenging ability''' and '''decreases oxidative damage'''<ref name="ref1"/>. | ||
| + | |||
| + | *The third ranked gene is Os06g0561000. From the GO annotation, its function is '''inferred to participate in biological process''' as '''inositol catabolic process''', '''L-ascorbic acid biosynthetic process''' and '''oxidation-reduction'''. Its | ||
| + | corresponding protein is placed in '''cytoplasm''' by GO that has molecular function as '''iron ion binding''', '''oxidoreductase activity''', '''metal ion binding''' and '''inositol oxygenase activity'''. It is expressed as unnamed inositol oxygenase | ||
| + | putatively<ref name="ref2"/><ref name="ref3"/>. | ||
===Evolution=== | ===Evolution=== | ||
| − | + | The ORF of OsMIOX obtained from UR variety, IRAT109, encodes a protein of 308 amino acids (aa). The BLAST analysis showed that the predicted amino acid sequence of the OsMIOX protein had '''high identity''' to other plant MIOX proteins, especially to Zea mays | |
| + | ('''87.99%'''), Vitis vinifera ('''75.56%''') and ''Arabidopsis thaliana'' ('''74.28%'''), and had '''43.69% identity''' to that from Homo sapiens, '''41.42% identity''' to that from Sus scrofa<ref name="ref1"/>. | ||
| − | + | ===Knowledge Extension=== | |
| + | [[File: myo-inositol Functional1.jpg|right|thumb|250px|'''Figure 1.''' ''Functional roles of myo-inositol in plant metabolism.(from reference <ref name="ref4"/>).'']] | ||
| + | *Fig. 1 summarizes this information by categorizing specific products of myo-inositol (MI) metabolism with particular interest to plant biologists and by identifying avenues of inquiry which may lead to a better appreciation of this unique molecule and its position in plant science<ref name="ref4"/>. | ||
| + | |||
| + | *Colored backgrounds in Fig. 1 attempt to provide a sense of related functions while avoiding the confusion of ‘metabolic mapping’. Beyond its inhibitory effect on myo-inositol (MI) monophosphatase, little is known regarding its impact on availability of free myo-inositol (MI) for numerous biosynthetic and regulatory requirements (Fig. 1)<ref name="ref4"/>. | ||
| + | |||
| + | *Lithium ion delayed initiation of DNA synthesis and cell division when introduced into synchronized Catharanthus roseus cell cultures, a condition largely prevented when MI was included in the medium<ref name="ref4"/>. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | *Key Lab of Crop Heterosis and Utilization of Ministry of Education, Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing 100193, PR China | |
| + | *International Rice Research Institute, Metro Manila, Philippines | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Duan J, Zhang M, Zhang H, et al. OsMIOX, a myo-inositol oxygenase gene, improves drought tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.)[J]. Plant Science, 2012, 196: 143-151. | ||
| + | </ref> | ||
| + | * <ref name="ref2"> | ||
| + | Wang J, Zhang F, Wang Y, et al. Identification of Salt Tolerance Genes in Rice from Microarray Data using SVM-RFE[C]//BICoB. 2011: 30-35. | ||
| + | </ref> | ||
| + | * <ref name="ref3"> | ||
| + | Tanaka T, Antonio B A, Kikuchi S, et al. The rice annotation project database (RAP-DB): 2008 update[J]. Nucleic acids research, 2008, 36(Supp 1): D1028-D1033. | ||
| + | </ref> | ||
| + | * <ref name="ref4"> | ||
| + | Loewus F A, Murthy P P N. myo-Inositol metabolism in plants[J]. Plant Science, 2000, 150(1): 1-19. | ||
| + | </ref> | ||
| + | </references> | ||
| + | |||
==Structured Information== | ==Structured Information== | ||
Revision as of 06:21, 5 March 2015
OsMIOX, a myo-inositol oxygenase gene, improves drought tolerance through scavenging of reactive oxygen species in rice[1].
Contents
Annotated Information
Function
OsMIOX has a specific function in drought stress tolerance by decreasing oxidative damage. The expression levels of ROS scavenging genes, CatB, POD1, POD2, APX1 and APX2 were indeed up-regulated, which may contribute to decrease the accumulation of ROS in OsMIOX-overexpressing plants under drought stress[1].
Mutation
Transgenic lines and wild type[1]:
- The transgenic rice lines overexpressing OsMIOX showed obviously improved growth performance in the medium containing 200 mM mannitol.
- Further, the survival rate of leaves from the transgenic rice lines was significantly higher than that of the wild type plants under polyethylene glycol treatment.
- It was discovered that the activity of ROS scavenging enzymes and proline content, as well as the transcript levels of many ROS scavenging genes were significantly increased in transgenic plants compared to the wild type plants under drought stress conditions.
- Four-week-old transgenic homozygous lines and the WT were treated with 20% PEG6000 for 6 days and then allowed to recover for 17 days.
- The transgenic lines recovered more quickly than the WT, and showed a total recovery of 38–46%, while the WT plants were severely affected by drought stress and had only 18–22% recovery.
- The water loss was determined by comparing the rates of change in fresh weight of the detached leaves. The water loss assay showed that the transgenic lines had a slower dehydration rate compared to WT, with a 0.28–2.6% lag from the beginning to 6 h after dehydration.
Expression
- OsMIOX was expressed predominantly in the roots and induced by drought, H2O2, salt, cold and abscisic acid. OsMIOX was more strongly induced in IRAT109 than that in Nipponbare under all treatments except for cold. The expression peak of OsMIOX was later in IRAT109 than that in Nipponbare under all treatments except salt and cold, indicating that the damage in UR was weaker and occurred later than that in LR, which suggesting that OsMIOX may be involved in abiotic stress responses, and related to drought tolerance in plant[1].
- The GUS activity was detected in spikelets, stem, leaves, especially in roots, and the activity was strongly induced by drought stress, indicating that OsMIOX was expressed in all rice organs and induced by drought stress.
Overexpression of OsMIOX increases free proline contents under drought stress and increases the ROS-scavenging ability and decreases oxidative damage[1].
- The third ranked gene is Os06g0561000. From the GO annotation, its function is inferred to participate in biological process as inositol catabolic process, L-ascorbic acid biosynthetic process and oxidation-reduction. Its
corresponding protein is placed in cytoplasm by GO that has molecular function as iron ion binding, oxidoreductase activity, metal ion binding and inositol oxygenase activity. It is expressed as unnamed inositol oxygenase putatively[2][3].
Evolution
The ORF of OsMIOX obtained from UR variety, IRAT109, encodes a protein of 308 amino acids (aa). The BLAST analysis showed that the predicted amino acid sequence of the OsMIOX protein had high identity to other plant MIOX proteins, especially to Zea mays (87.99%), Vitis vinifera (75.56%) and Arabidopsis thaliana (74.28%), and had 43.69% identity to that from Homo sapiens, 41.42% identity to that from Sus scrofa[1].
Knowledge Extension
- Fig. 1 summarizes this information by categorizing specific products of myo-inositol (MI) metabolism with particular interest to plant biologists and by identifying avenues of inquiry which may lead to a better appreciation of this unique molecule and its position in plant science[4].
- Colored backgrounds in Fig. 1 attempt to provide a sense of related functions while avoiding the confusion of ‘metabolic mapping’. Beyond its inhibitory effect on myo-inositol (MI) monophosphatase, little is known regarding its impact on availability of free myo-inositol (MI) for numerous biosynthetic and regulatory requirements (Fig. 1)[4].
- Lithium ion delayed initiation of DNA synthesis and cell division when introduced into synchronized Catharanthus roseus cell cultures, a condition largely prevented when MI was included in the medium[4].
Labs working on this gene
- Key Lab of Crop Heterosis and Utilization of Ministry of Education, Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing 100193, PR China
- International Rice Research Institute, Metro Manila, Philippines
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Duan J, Zhang M, Zhang H, et al. OsMIOX, a myo-inositol oxygenase gene, improves drought tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.)[J]. Plant Science, 2012, 196: 143-151.
- ↑ Wang J, Zhang F, Wang Y, et al. Identification of Salt Tolerance Genes in Rice from Microarray Data using SVM-RFE[C]//BICoB. 2011: 30-35.
- ↑ Tanaka T, Antonio B A, Kikuchi S, et al. The rice annotation project database (RAP-DB): 2008 update[J]. Nucleic acids research, 2008, 36(Supp 1): D1028-D1033.
- ↑ 4.0 4.1 4.2 4.3 Loewus F A, Murthy P P N. myo-Inositol metabolism in plants[J]. Plant Science, 2000, 150(1): 1-19.
Structured Information
| Gene Name |
Os06g0561000 |
|---|---|
| Description |
Similar to Myo-inositol oxygenase |
| Version |
NM_001064406.1 GI:115468543 GeneID:4341305 |
| Length |
3905 bp |
| Definition |
Oryza sativa Japonica Group Os06g0561000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 6:22355480..22359384 |
| Sequence Coding Region |
22355662..22355692,22355836..22355950,22357051..22357132,22357267..22357471,22358060..22358119 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008399:22355480..22359384 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008399:22355480..22359384 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgaccatcaccattgagcagcctcaccttgatgctatcgccgaccggaaggtcgccggcggcggcggcggcgacaacgcggcggagctcgtgctcgacggcggcttcgtcgtgccggactccaacgccttcggcaacgccttcaggaattatgaggccgagtctgagaggaaggagacggtggaggagttctaccgggtcaaccacatcaaccagacatatgatttcgtgaggcggatgcgggaggagtacggtagggtggacaagacggagatggggatctgggagtgcatcgagctgctcaacgagttcatcgacgacagcgacccggacctcgacatgccgcagatcgagcacctcctccagaccgccgaggccatccgcaaggatttccccgacgaggactggctccacctcactggcctcatccacgatctgggcaaggtgctgctgcatcccagctttggggagctcccacagtggtcagtcgtcggtgacaccttccccgtcggctgcgcattcgacgaatgcaacgtccacttcaagtacttcaaggagaaccctgactacctgaacccaaagctcaacaccaagtttggggcctactccgagggctgtggccttgacaatgttctcatgtcctggggccatgacgactacatgtacctggttgccaaggagaacaagaccactcttccttctgcaggcttgttcatcatcagatatcattcattctaccccctgcacaagcatggagcctacatgcatctgatgaacgatgaggacaaggagaacctcaaatggctgcgtgtgttcaacaaatacgacctgtacagcaagagcaatgagaggatagacgttgagaaggtgaagccctactacatgtcactcatcgaaaagtatttcccggccaagttgagatggtga</cdnaseq> |
| Protein Sequence |
<aaseq>MTITIEQPHLDAIADRKVAGGGGGDNAAELVLDGGFVVPDSNAF GNAFRNYEAESERKETVEEFYRVNHINQTYDFVRRMREEYGRVDKTEMGIWECIELLN EFIDDSDPDLDMPQIEHLLQTAEAIRKDFPDEDWLHLTGLIHDLGKVLLHPSFGELPQ WSVVGDTFPVGCAFDECNVHFKYFKENPDYLNPKLNTKFGAYSEGCGLDNVLMSWGHD DYMYLVAKENKTTLPSAGLFIIRYHSFYPLHKHGAYMHLMNDEDKENLKWLRVFNKYD LYSKSNERIDVEKVKPYYMSLIEKYFPAKLRW</aaseq> |
| Gene Sequence |
<dnaseqindica>183..213#357..471#1572..1653#1788..1992#2581..2640#2764..2816#2933..3055#3188..3257#3356..3434#3516..3597#3700..3726#agctcaggcagcagcagaaagcaacgccaatacgccattgccgtgctcctcgacctgcatgcacctctgttcttggcgttgagatacatagaggcttggggtgggtgtttaagaactaagaccggagttaattagcacccctgtggctaccatcgatctgtctaaagagtgattagatcgagatgaccatcaccattgagcagcctcaccttggtgagctcatgatccttttctctctctctcttgatttcttgattccagtccgtgaggaagaaaaggttcagcaatgttcctggatggatggatggatcgtggaactaattaaggttctaagttctgacatggttcgcttgcagatgctatcgccgaccggaaggtcgccggcggcggcggcggcgacaacgcggcggagctcgtgctcgacggcggcttcgtcgtgccggactccaacgccttcggcaacgccttcaggtcagctacatattcctgcataggaaaaaaacagagcatcatgcaaaaacagagcaaaacagagtgtcaactatggttaaaaatattatcccctcagaagaaaaaatatttaaaatatttctagtggattagggaatttaattaatcatgtagtatttttttaaatatggtgaaggttattgtttctctttgcaaaaaacagagtgggcagcaagttttttttcaagtggacatgtgcaaataatcatatattttgcatatggaatgtcttcttttttctcctttgcaaaaacagaggttggtattacttgctccctctaaaataagccaacatcgtattaagatatgatatattttagtacaacgttatatttagatttgttatattagaatgattggcttatttttaggacaatgcgagcaggctgacaagacagacatgatattttcgtacgaaattagtaaactagaacatgggcacaactcgaaatgaaagagatgtgtggccattcttgaaacttgtggttgtgcattcaaatcgtacatacctctctctcaagccacaatacatgtttgtcccattacttcaagttgtctttccatgattagccgtttggtttagtttttgttgaaacgtccaaacccaggttttgtttttttttcagataaaactggacaagaagaagatgaatttgactacttaaacgtaaactcaattttgctgaccaaagtggcaagctacttactggtcaaataaaataaaataaagacttatcttttcttgtacgatttcaaaggtttctgcgtgtctatgcacagtgaatgtcccacgtggcatctgtggctgcattaacatggccttttgcctattccttttttgcacgttatcattttcgcttcttcaggaattctgtacttcaatttgatgtttctttaaaaagtccaacacgtggacaaataaaacactacctgtgttcgaaaaataaaaaccttatggtcataatctagaaattgaaatctttttcaacggtggtagtaaaagatatttcttaaaaaaaaggaattaaccggaataatatggtgatgttttaaatctccaggaattatgaggccgagtctgagaggaaggagacggtggaggagttctaccgggtcaaccacatcaaccagacatatgatttcgtaagtgcaaattctgtatctaataatctttttttaaaaaaatacactcataatatcctaaacgacaagtaaaaaagaacgggagctttaatatatgagatttaccattattttggtgcattgtaacattgcaggtgaggcggatgcgggaggagtacggtagggtggacaagacggagatggggatctgggagtgcatcgagctgctcaacgagttcatcgacgacagcgacccggacctcgacatgccgcagatcgagcacctcctccagaccgccgaggccatccgcaaggatttccccgacgaggactggctccacctcactggcctcatccacggtaaattaaactattttataaccgccacaaaaaccgtcactgtaattgcacatccaccgcgtcgccgcaatgaactgataggcaggcctcacacgatacaccgggtaggatttgttggtgtaagagcaggtacaatagcaggctattagccagctataaatatattttaatgagataaacgatgagagagaagagtagtgggctacagatctgtagccagctgcagcacggacttcaagacgcaatgtgtgtatgacaggtgataccatatattaatagtatagtaagcaactattgaatgaattggctattagattagctatagatgaattggagctagtagtgggctatactattaaacttgctctaaggtagtaataatagtatatatctaggactcatccagtcactagcaggtaggccatacgataaataaagatgaataattagatatgatggagtgatacagtatcagtgacggttcgtaacaacctctttggaattactcggctaaaggaagaaagaaatgaaatggcaatacagtggggcacacttgtgacgtgtaacatgttctcttttctttttcagatctgggcaaggtgctgctgcatcccagctttggggagctcccacagtggtcagtcgtcggtaagaaacaaacaacagaagcaaacagcttattgccatctcactgaaacttcttcagattcagctaggaacaatagctgaacatccatccatctctgaatctgaatgtctttgacctttcaggtgacaccttccccgtcggctgcgcattcgacgaatgcaacgtccacttcaaggtaaccaatgaatcatctagcaaactgttcaagacaaatcaaaagcgtagatggatcaagcagtgagctagtggtctaagactgaaactgaaaatattttgtctgaaaatgtgcagtacttcaaggagaaccctgactacctgaacccaaagctcaacaccaagtttggggcctactccgagggctgtggccttgacaatgttctcatgtcctggggccatgacgactacatgtacctggtaaaacacacggttcatcgtgtcgattcatcaccaattgcataccatggagcagagaaatcttcagttctagtactgaggttgtagttttctctgaaatgttgatgtgtttcttgttcttgttcatctcaggttgccaaggagaacaagaccactcttccttctgcaggcttgttcatcatcagatatcattcattctaccgtaagtgcgcgcgacagtcttctttttttcaattttgtcagaattacaagttttaagtaagttttagctaaattttgctatctttgtccatgattcagccctgcacaagcatggagcctacatgcatctgatgaacgatgaggacaaggagaacctcaaatggctgcgtgtgttcaagtaagccaccagattgatcaccctgcatcttaatgtatatagtatgaaaatgtactgaacatgaatggttttcttctgcagcaaatacgacctgtacagcaagagcaatgagaggatagacgttgagaaggtgaagccctactacatgtcactcatcgaaaaggtatgatcgattttgcagcgaatactgaagcttgaaacagacttctttttgtcctaaaacctgaagaaatgatcttctttttctctctgaattctctcgcagtatttcccggccaagttgagatggtgaagaagagggagagaacataaaaaaaattggtcgaaattatcctgtaatatgatgtaattgcaggagttcagttggctgtacaagtatgtagtagtacgtgtacacattttgtatgttaagagcagaatttgttgggagccatgcacgtggaataaaagttgccggtgattgttctttgt</dnaseqindica> |
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