Difference between revisions of "Os03g0427300"
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===Function=== | ===Function=== | ||
===Mutation=== | ===Mutation=== | ||
| − | * Using RT-PCR in the presence of dUTPOregon | + | * Using RT-PCR in the presence of dUTPOregon Green to label RNAs and rhodamine B hexyl ester to stain prolamine protein bodies, glutelin RNAs are found to be preferentially transported to the cisternal-ER (Cis-ER) in wildtype endosperm (Fig. 1). Prolamine RNAs, on the other hand, are targeted to ER that delimits the spherical prolamine protein bodies (PB-ER). In the glup4 mutant, however, glutelin RNAs are partially misdirected from the Cis-ER to the PB-ER (Fig. 1), as well as to large dilated multivesicular body-like structures located near the plasma membrane (data not shown). The glutelin RNA mistargeting was not only observed in one gene (Gt-2), but also in others (GluA-1, GluA- 3 and GluB-1, data not shown). Prolamine RNA localization to the PB-ER is unaffected (Fig. 1), suggesting that Rab5 is involved in glutelin RNA sorting only. |
| − | Green to label RNAs and rhodamine B hexyl ester to | ||
| − | stain prolamine protein bodies, glutelin RNAs are found to | ||
| − | be preferentially transported to the cisternal-ER (Cis-ER) in | ||
| − | wildtype endosperm (Fig. 1). Prolamine RNAs, on the other | ||
| − | hand, are targeted to ER that delimits the spherical prolamine | ||
| − | protein bodies (PB-ER). In the glup4 mutant, however, | ||
| − | glutelin RNAs are partially misdirected from the Cis-ER to | ||
| − | the PB-ER (Fig. 1), as well as to large dilated multivesicular | ||
| − | body-like structures located near the plasma membrane (data not shown). The glutelin RNA mistargeting was not only observed | ||
| − | in one gene (Gt-2), but also in others (GluA-1, GluA- | ||
| − | 3 and GluB-1, data not shown). Prolamine RNA localization | ||
| − | to the PB-ER is unaffected (Fig. 1), suggesting that Rab5 is | ||
| − | involved in glutelin RNA sorting only. | ||
[[File:Os03g0427300-1.png|center|thumb|400px|'''Fig. 1. Localization of prolamine and glutelin mRNAs in wildtype and glup4 developing rice endosperm.''' '' <ref name="ref1" />.'']] | [[File:Os03g0427300-1.png|center|thumb|400px|'''Fig. 1. Localization of prolamine and glutelin mRNAs in wildtype and glup4 developing rice endosperm.''' '' <ref name="ref1" />.'']] | ||
Revision as of 03:25, 5 August 2016
Glup4 gene encodes small GTPase, Rab5a in rice[1].
Contents
Annotated Information
Function
Mutation
- Using RT-PCR in the presence of dUTPOregon Green to label RNAs and rhodamine B hexyl ester to stain prolamine protein bodies, glutelin RNAs are found to be preferentially transported to the cisternal-ER (Cis-ER) in wildtype endosperm (Fig. 1). Prolamine RNAs, on the other hand, are targeted to ER that delimits the spherical prolamine protein bodies (PB-ER). In the glup4 mutant, however, glutelin RNAs are partially misdirected from the Cis-ER to the PB-ER (Fig. 1), as well as to large dilated multivesicular body-like structures located near the plasma membrane (data not shown). The glutelin RNA mistargeting was not only observed in one gene (Gt-2), but also in others (GluA-1, GluA- 3 and GluB-1, data not shown). Prolamine RNA localization to the PB-ER is unaffected (Fig. 1), suggesting that Rab5 is involved in glutelin RNA sorting only.
Fig. 1. Localization of prolamine and glutelin mRNAs in wildtype and glup4 developing rice endosperm. [2].
Expression Pattern
Evolution
Subcellular localization
Labs working on this gene
- Institute of Biological Chemistry, Washington State University, Pullman WA, 99164-6340 USA
- Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan
- School of Molecular Biosciences, Washington State University, Pullman WA, 99164-7520 USA
- Present Address: International Liberal Arts Program, Akita International University, Akita 010-1292, Japan
- Present Address: Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
References
- ↑ Satoh-Cruz M, Fukuda M, Ogawa M, et al. Glup4 gene encodes small GTPase, Rab5a in rice[J]. Rice Genet Newsl, 2010, 25: 48-49.
- ↑ Doroshenk K A, Crofts A J, Washida H, et al. Characterization of the rice glup4 mutant suggests a role for the small GTPase Rab5 in the biosynthesis of carbon and nitrogen storage reserves in developing endosperm[J]. Breeding science, 2010, 60(5): 556-567.
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