Difference between revisions of "Os03g0427300"
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===Function=== | ===Function=== | ||
===Mutation=== | ===Mutation=== | ||
| − | * Using RT-PCR in the presence of dUTPOregon Green to label RNAs and rhodamine B hexyl ester to stain prolamine protein bodies, glutelin RNAs are found to be preferentially transported to the cisternal-ER (Cis-ER) in wildtype endosperm (Fig. 1). Prolamine RNAs, on the other hand, are targeted to ER that delimits the spherical prolamine protein bodies (PB-ER). In the glup4 mutant, however, glutelin RNAs are partially misdirected from the Cis-ER to the PB-ER (Fig. 1), as well as to large dilated multivesicular body-like structures located near the plasma membrane (data not shown). The glutelin RNA mistargeting was not only observed in one gene (Gt-2), but also in others (GluA-1, GluA- 3 and GluB-1, data not shown). Prolamine RNA localization to the PB-ER is unaffected (Fig. 1), suggesting that Rab5 is involved in glutelin RNA sorting only. | + | * Using RT-PCR in the presence of dUTPOregon Green to label RNAs and rhodamine B hexyl ester to stain prolamine protein bodies, glutelin RNAs are found to be preferentially transported to the cisternal-ER (Cis-ER) in wildtype endosperm (Fig. 1). Prolamine RNAs, on the other hand, are targeted to ER that delimits the spherical prolamine protein bodies (PB-ER). In the glup4 mutant, however, glutelin RNAs are partially misdirected from the Cis-ER to the PB-ER (Fig. 1), as well as to large dilated multivesicular body-like structures located near the plasma membrane (data not shown). The glutelin RNA mistargeting was not only observed in one gene (Gt-2), but also in others (GluA-1, GluA- 3 and GluB-1, data not shown). Prolamine RNA localization to the PB-ER is unaffected (Fig. 1), suggesting that Rab5 is involved in glutelin RNA sorting only<ref name="ref1" />. |
[[File:Os03g0427300-1.png|center|thumb|400px|'''Fig. 1. Localization of prolamine and glutelin mRNAs in wildtype and glup4 developing rice endosperm.''' '' <ref name="ref1" />.'']] | [[File:Os03g0427300-1.png|center|thumb|400px|'''Fig. 1. Localization of prolamine and glutelin mRNAs in wildtype and glup4 developing rice endosperm.''' '' <ref name="ref1" />.'']] | ||
| + | |||
| + | * Microarray analysis of transcripts isolated from glup4 and wildtype developing seeds identified 35 transcripts that | ||
| + | were upregulated and 10 that were downregulated by at least 2-fold in the glup4 mutant using a stringent P value (<0.001) to avoid false positives (Table 1 and Table 2)<ref name="ref1" />. | ||
| + | |||
| + | [[File:Os03g0427300-2.png|center|thumb|400px|'''Table 1. Transcripts upregulated in the glup4 mutant compared to wildtype (P < 0.001)''' '' <ref name="ref1" />.'']] | ||
| + | |||
| + | [[File:Os03g0427300-3.png|center|thumb|400px|'''Table 2. Transcripts downregulated in the glup4 mutant compared to | ||
| + | wildtype (P < 0.001)''' '' <ref name="ref1" />.'']] | ||
| + | |||
| + | * The researchers utilized | ||
| + | 2D-DIGE to analyze the protein expression profiles of | ||
| + | soluble extracts from 12–14 DAF wildtype and glup4 rice | ||
| + | seeds (Fig. 3). Twenty-one protein spots were found to be | ||
| + | differentially expressed (P < 0.05) with at least a 2-fold | ||
| + | change in expression when analyzed on pH 3–10 non-linear | ||
| + | immobilized pH gradient strips. Only two proteins were found to have at least a 2-fold | ||
| + | increase in expression in the glup4 mutant (Table 3). One of these proteins, annotated as a glycosyl hydrolase, has similarity | ||
| + | to xylanase inhibitor (XI) proteins, which function in | ||
| + | cell wall restructuring<ref name="ref3" />. Although not | ||
| + | the same protein, xylanase inhibitor TAXI-VI mRNA was | ||
| + | also found to be upregulated in the mutant (Table 1). | ||
===Expression Pattern=== | ===Expression Pattern=== | ||
Revision as of 03:44, 5 August 2016
Glup4 gene encodes small GTPase, Rab5a in rice[1].
Contents
Annotated Information
Function
Mutation
- Using RT-PCR in the presence of dUTPOregon Green to label RNAs and rhodamine B hexyl ester to stain prolamine protein bodies, glutelin RNAs are found to be preferentially transported to the cisternal-ER (Cis-ER) in wildtype endosperm (Fig. 1). Prolamine RNAs, on the other hand, are targeted to ER that delimits the spherical prolamine protein bodies (PB-ER). In the glup4 mutant, however, glutelin RNAs are partially misdirected from the Cis-ER to the PB-ER (Fig. 1), as well as to large dilated multivesicular body-like structures located near the plasma membrane (data not shown). The glutelin RNA mistargeting was not only observed in one gene (Gt-2), but also in others (GluA-1, GluA- 3 and GluB-1, data not shown). Prolamine RNA localization to the PB-ER is unaffected (Fig. 1), suggesting that Rab5 is involved in glutelin RNA sorting only[2].
Fig. 1. Localization of prolamine and glutelin mRNAs in wildtype and glup4 developing rice endosperm. [2].
- Microarray analysis of transcripts isolated from glup4 and wildtype developing seeds identified 35 transcripts that
were upregulated and 10 that were downregulated by at least 2-fold in the glup4 mutant using a stringent P value (<0.001) to avoid false positives (Table 1 and Table 2)[2].
Table 1. Transcripts upregulated in the glup4 mutant compared to wildtype (P < 0.001) [2].
Table 2. Transcripts downregulated in the glup4 mutant compared to wildtype (P < 0.001) [2].
- The researchers utilized
2D-DIGE to analyze the protein expression profiles of soluble extracts from 12–14 DAF wildtype and glup4 rice seeds (Fig. 3). Twenty-one protein spots were found to be differentially expressed (P < 0.05) with at least a 2-fold change in expression when analyzed on pH 3–10 non-linear immobilized pH gradient strips. Only two proteins were found to have at least a 2-fold increase in expression in the glup4 mutant (Table 3). One of these proteins, annotated as a glycosyl hydrolase, has similarity to xylanase inhibitor (XI) proteins, which function in cell wall restructuring[3]. Although not the same protein, xylanase inhibitor TAXI-VI mRNA was also found to be upregulated in the mutant (Table 1).
Expression Pattern
Evolution
Subcellular localization
Labs working on this gene
- Institute of Biological Chemistry, Washington State University, Pullman WA, 99164-6340 USA
- Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan
- School of Molecular Biosciences, Washington State University, Pullman WA, 99164-7520 USA
- Present Address: International Liberal Arts Program, Akita International University, Akita 010-1292, Japan
- Present Address: Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan
References
- ↑ Satoh-Cruz M, Fukuda M, Ogawa M, et al. Glup4 gene encodes small GTPase, Rab5a in rice[J]. Rice Genet Newsl, 2010, 25: 48-49.
- ↑ 2.0 2.1 2.2 2.3 2.4 Doroshenk K A, Crofts A J, Washida H, et al. Characterization of the rice glup4 mutant suggests a role for the small GTPase Rab5 in the biosynthesis of carbon and nitrogen storage reserves in developing endosperm[J]. Breeding science, 2010, 60(5): 556-567.
- ↑
