Difference between revisions of "Os02g0101500"

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==Annotated Information==
 
==Annotated Information==
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[[File:Os02g0101500-1.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.<ref name="ref1" />.'']]
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[[File:Os02g0101500.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.<ref name="ref1" />.'']]
 
===Gene Symbol===
 
===Gene Symbol===
 
*'''''Os02g0101500''''' '''<=>''' '''''OsHPR1''''','''''HPR1'''''
 
*'''''Os02g0101500''''' '''<=>''' '''''OsHPR1''''','''''HPR1'''''

Latest revision as of 13:10, 3 October 2016

The rice Os02g0101500 was reported as OsHPR1 in 2014 [1] by researchers from China.

Annotated Information

Figure 4. Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.[1].

Gene Symbol

  • Os02g0101500 <=> OsHPR1,HPR1

Function

  • The researchers constructed over-expression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice.
  • The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line.
  • Double deletion of OsHPR1 and OsHPR2 genes significantly reduces plant growth under normal photorespiration condition
  • These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.

Expression

  • Results from RT-PCR, western-blot and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity, which is the same for HPR2.

Subcellular Localization

  • In the present study, the subcellular localization of OsHPR1 protein was first predicted to be located in the peroxisome and cytosol respectively.
  • In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome.


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Labs working on this gene

  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
  • The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
  • State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China

References

  1. ↑ 1.0 1.1 Ye N, Yang G, Chen Y, Zhang C, Zhang J, Peng X. Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. J Integr Plant Biol. 2014 Feb;56(2):170-80. doi: 10.1111/jipb.12125. Epub 2014 Jan 8. PubMed PMID: 24401104.

Structured Information