Difference between revisions of "Os05g0154600"
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| − | + | The rice '''''Os05g0154600''''' was reported as '''''APIP6''''' in 2012 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:107-Os05g0154600.png|right|thumb|427px|'''Figure 2.''' ''Response of AvrPiz-t Transgenic Rice to Blast Infection, flg22, and Chitin Treatments.<ref name="ref1" />.'']] | ||
===Function=== | ===Function=== | ||
| − | + | * '''''APIP6''''' in is a Functional RING Finger E3 Ubiquitin Ligase | |
| − | + | * AvrPiz-t suppresses the ubiquitin ligase activity of the rice RING E3 ubiquitin ligase '''''APIP6''''' and that, in return, APIP6 ubiquitinates AvrPiz-t in vitro. | |
| + | * Agroinfection assays reveal that AvrPiz-t and AvrPiz-t Interacting Protein 6 ('''''APIP6''''') are both degraded when coexpressed in Nicotiana benthamiana. | ||
| + | * Silencing of '''''APIP6''''' in transgenic rice leads to a significant reduction of flg22-induced ROS generation, suppression of defense-related gene expression, and enhanced susceptibility of rice plants to M. oryzae. | ||
===Expression=== | ===Expression=== | ||
| − | + | * In both the T1 (primary transformants) and T2 generations, the RNAi plants grew normally and did not show any changes in growth and development. | |
| − | + | * Blast inoculations showed that the APIP6 RNAi plants were as resistant as the wild-type Piz-t plants (TRD) to the isolate, suggesting that APIP6 is not required for the Piz-t-mediated resistance to the avirulent strain. | |
| − | + | * Punch inoculation assays showed that lesions were larger on the APIP6 RNAi plants than on the segregated wild-type plants (Figure 7A). | |
| − | |||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * Department of Plant Pathology, Ohio State University, Columbus, OH 43210 | |
| + | * State Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural | ||
| + | Sciences, Beijing 100193, China | ||
| + | * Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China | ||
| + | * State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021 China | ||
| + | * Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506 | ||
| + | * Department of Horticulture and Crop Science, Ohio State University, Columbus, OH 43210 | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Park, Chan-Ho, et al. "The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 Ubiquitin Ligase APIP6 to suppress pathogen-associated molecular pattern–triggered immunity in rice." The Plant Cell 24.11 (2012): 4748-4762. | ||
| + | </ref> | ||
| + | </references> | ||
| + | |||
==Structured Information== | ==Structured Information== | ||
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]] | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]] | ||
Revision as of 15:39, 17 October 2016
The rice Os05g0154600 was reported as APIP6 in 2012 [1] by researchers from China.
Contents
Annotated Information
Figure 2. Response of AvrPiz-t Transgenic Rice to Blast Infection, flg22, and Chitin Treatments.[1].
Function
- APIP6 in is a Functional RING Finger E3 Ubiquitin Ligase
- AvrPiz-t suppresses the ubiquitin ligase activity of the rice RING E3 ubiquitin ligase APIP6 and that, in return, APIP6 ubiquitinates AvrPiz-t in vitro.
- Agroinfection assays reveal that AvrPiz-t and AvrPiz-t Interacting Protein 6 (APIP6) are both degraded when coexpressed in Nicotiana benthamiana.
- Silencing of APIP6 in transgenic rice leads to a significant reduction of flg22-induced ROS generation, suppression of defense-related gene expression, and enhanced susceptibility of rice plants to M. oryzae.
Expression
- In both the T1 (primary transformants) and T2 generations, the RNAi plants grew normally and did not show any changes in growth and development.
- Blast inoculations showed that the APIP6 RNAi plants were as resistant as the wild-type Piz-t plants (TRD) to the isolate, suggesting that APIP6 is not required for the Piz-t-mediated resistance to the avirulent strain.
- Punch inoculation assays showed that lesions were larger on the APIP6 RNAi plants than on the segregated wild-type plants (Figure 7A).
You can also add sub-section(s) at will.
Labs working on this gene
- Department of Plant Pathology, Ohio State University, Columbus, OH 43210
- State Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural
Sciences, Beijing 100193, China
- Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
- State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021 China
- Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506
- Department of Horticulture and Crop Science, Ohio State University, Columbus, OH 43210
References
