Difference between revisions of "Os02g0765600"
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==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * National Agricultural Research Center, Joetsu, Japan | |
| − | + | * RIKEN Plant Science Center, Tsurumi, Yokohama, Japan | |
| − | + | * Niigata University, Ikarashi, Niigata, Japan | |
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==References== | ==References== | ||
| − | + | <references> | |
| − | + | * <ref name="ref1"> | |
| − | + | Hakata M, Kuroda M, Miyashita T, et al. Suppression of α-amylase genes improves quality of rice grain ripened under high temperature.[J]. Plant Biotechnology Journal, 2012, 10(9):1110–1117. | |
| − | + | </ref> | |
| − | + | </references> | |
==Structured Information== | ==Structured Information== | ||
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]] | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]] | ||
Revision as of 09:07, 9 November 2016
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Contents
Annotated Information
Function
Alpha-amylase gene, RAmylA, encodes rice α-amylase isoform A.
Expression
A significant difference in the seed germinability was observed between the two rice cultivars, 'Nipponbare' and 'Suweon 287', under anoxia (i.e., during germination in submerged soil at 18℃), although little difference was seen under aerobic (in air) or hypoxic (in water) conditions. The number of α-amylase isoforms synthesized in germinating seeds was inversely proportional to the O2 concentrations at the early germination stage. The formation of isoform B was promoted by oxygen supply, while isoform H was undetectable if the seeds were unable to germinate. The activity of isoform H was highly correlated with the coleoptile length in the submerged soil at 18℃, indicating that isoform H is a critical factor for seed germination under anoxia. The expression of the rice α-amylase RAmy1A gene was repressed when the seeds germinated under hypoxia or anoxia[1]. Northern blot analysis and RNA-PCR were used to detect the expression of α-amylase genes in various tissues. Alpha-amylase mRNA was abundant in germinating seeds and callus. RAmy1A and RAmy3E were expressed in all tissues and RAmy1A transcript was most abundant in the germinating seeds[2]. And RAmy1A was demonstrated to be sugar tepressed in rice embryos and functional dissection of the promoter of RAmy1A in relation of its sugar-modulated expression was performed[3].
Fig. 1. Effect of glucose on mRNA levels of α-amylase genes (RAmy1A and RAmy3D) in rice embryos. Embryos dissected from the endosperm (control; lane 1) were incubated for 1 day on a glucose-free medium (1 DS, 1 day starvation; lane 2). After 1 day starvation, embryos were incubated for additional 2 days on a glucose-free medium (3 DS, 3 day starvation; lane 3), or for 2 days on a glucose-containing medium at the concentration of 10 mM (+Glc, 10 mM; lane 4), 30 mM (+Glc, 30 mM; lane 5) and 90 mM (+Glc, 90 mM; lane 6). A: Pattern and quantitation of RAmy1A mRNA level. Relative unit is expressed as lane 3: 100. B: Pattern and quantitation of RAmy3D mRNA level. C: Pattern of rRNA level. D: Glucose content in rice embryos used in the experiment. Data were derived from a part of Table 1. Relative glucose content is expressed as lane 6: 100.
Ontology
Gene ontology: The chemical reactions and pathways involve in carbohydrates.
Growth stage ontology: It occurs when the seed coat has imbibed adequate water becoming soft and elastic.
Labs working on this gene
- National Agricultural Research Center, Joetsu, Japan
- RIKEN Plant Science Center, Tsurumi, Yokohama, Japan
- Niigata University, Ikarashi, Niigata, Japan
References
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