Difference between revisions of "Os03g0315400"

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===Expression===
 
===Expression===
 
* Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
 
* Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
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[[File:Subcellular localization of OsMYB2.jpg]]
 
  
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a、b、c: localization of GFP ;  e、d、f:localization of GFP-OsMYB2
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===Subcellular localization===
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* OsMYB2 was localized in the nucleus with transactivation activity.
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<br>
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[[File:Subcellular localization of OsMYB2.jpg|center|thumb|800px|'''Figure 1.''' ''Subcellular localization and transactivation analysis of OsMYB2.<ref name="ref1" />.'']]
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The response of OsMYB2 expression to salt, cold, and
 
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dehydration stress was monitored by real-time RT-PCR.
 
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An increase in the OsMYB2 transcript was observed after
 
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30 min of exposure to salt stress. The salt stress-induced
 
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increase in the OsMYB2 transcript peaked after 5 h of salt
 
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stress, and thereafter the transcript declined gradually under
 
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salt stress . A similar increase in the OsMYB2
 
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transcript was also observed when rice seedlings were
 
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exposed to low temperature (2℃) or osmotic stress (20%
 
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PEG) . In addition, treatment of rice seedlings with ABA
 
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also led to an increase in expression of OsMYB2. In contrast,
 
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exogenous application of salicylic acid reduced the expression of OsMYB2, while no effect of
 
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indoleacetic acid and brassinosteroids on the OsMYB2
 
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transcript was observed. OsMYB2 was detected in roots,
 
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shoots, leaves, and flowers under non-stressed conditions,
 
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with the expression being greatest in leaves, followed by
 
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roots and shoots . The strong induction of this
 
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gene by abiotic stress prompted this study to check its
 
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promoter sequence (1500 bp upstream from the transcription
 
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start site) by searching the promoter sequence against
 
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the PLACE database [(http://www.dna.affrc.go.jp/PLACE/)].
 
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The promoter of OsMYB2 contains stress-responsive related
 
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cis-elements, such as ABRE and MYB and MYC
 
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recognition sites
 
  
 
[[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg]]
 
[[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg]]
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===Evolution===
 
===Evolution===

Revision as of 01:46, 14 February 2017

The rice Os03g0315400 was reported as OsMYB2 in 2012 [1] by researchers from China.

Annotated Information

Gene Symbol

  • Os03g0315400 <=> OsMYB2,MYB2

Function

  • OsMYB2 encodes a stress-responsive MYB transcription factor.
  • OsMYB2 plays a regulatory role in tolerance of rice to salt, cold, and dehydration stress.

Phenotypic analysis

  • No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants.
  • The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H 2 O 2 and malondialdehyde.
  • The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants.
  • There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants.

Expression

  • Expression of OsMYB2 was up-regulated by salt, cold, and dehydration stress.

Subcellular localization

  • OsMYB2 was localized in the nucleus with transactivation activity.


Figure 1. Subcellular localization and transactivation analysis of OsMYB2.[1].




Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg

Evolution

Phylogenetic tree of MYB proteins(figure 6).


Phylogenetic tree of MYB proteins.jpg

OsMYB2 representative by LOC_Os3g20090 is belong to C12, and of which are involved in stress response.

Labs working on this gene

  • State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China
  • Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China

References

  1. ↑ 1.0 1.1 Yang A, Dai X, Zhang WH. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot. 2012 Apr;63(7):2541-56. doi: 10.1093/jxb/err431. PubMed PMID: 22301384; PubMed Central PMCID: PMC3346221.

Structured Information