Difference between revisions of "Os06g0181700"

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Please input one-sentence summary here.
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The rice '''''Os06g0181700''''' was reported as '''''OsERF#002''''' in 2006 <ref name="ref1" /> by researchers from Japan.  
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os06g0181700''''' '''''<=>''''' '''''OsERF#002'''''
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===Function===
 
===Function===
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ERF proteins, Arabidopsis WIN1/SHN1 and Medicago WXP1/2, have been shown to induce the production of epidermal waxes when overexpressed in plants.
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* Genes in the ERF family encode transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants.
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* It has been demonstrated that the AP2/ERF proteins have important functions in the transcriptional regulation of a variety of biological processes related to growth and development, as well as various responses to environmental stimuli.
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The WIN1-like OsWR1 controls wax formation by direct or collaborative activation of wax synthesis genes, and this regulation, distinct from its homology  regulator of WIN1/SHN1 in cutin synthesis, subsequently results in reduced water loss and enhanced drought tolerance.
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* Genes in the AP2 family have been shown to participate in the regulation of developmental processes, e.g. flower development (Elliott et al., 1996), spikelet meristem determinacy (Chuck et al., 1998), leaf epidermal cell identity (Moose and Sisco, 1996), and embryo development (Boutilier et al., 2002).<ref name="ref2" />
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To investigate the functional role of
 
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this region,Youhua Wang fused the coding regions of OsWR1 and its
 
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deletion of the predicted acidic activation region to the DNA-binding-domain expres-
 
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sion vector of GAL4 and examined the behavior of GAL4–
 
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OsWR1 and GAL4–OsWR1–DAD constructs as potential
 
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transcriptional activators in yeast. In the presence of the
 
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activation domain, the OsWR1 protein fused to the DNA-
 
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binding domain activated transcription of the lacZ reporter gene,
 
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whereas GAL4–OsWR1–DAD  could not, indicating that the OsWR1 protein may
 
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act as a transcriptional activator.
 
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Datas reveal that OsWR1 is responsive to drought, salt and ABA in rice.
 
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OsWR1 is a positive regulator for drought response through affecting water loss.
 
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OsWR1 might participate in the regulation of oxidative
 
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stress response and membrane stability, and this regulation
 
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might be a compensation for the modulation of OsWR1 in
 
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drought response.
 
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OsWR1
 
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that at least partially directly activates the expression of
 
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wax synthesis related genes to enhance wax production,
 
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through the regulation of the components of wax biosyn-
 
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thesis. This indicate that
 
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OsWR1 is a positive regulator of wax synthesis in rice.
 
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OsWR1 modulates the expression of wax biosynthesis
 
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related genes in rice and  OsWR1 physically interacts with the
 
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GCC box and DRE in the wax synthesis related gene
 
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promoters.
 
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===Expression===
 
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To test nuclear localization, an in vivo targeting experiment was performed by fusing the coding region of OsWR1 to GFP, and the resulting constructs (WR1–GFP and GFP only) were introduced into rice calli by agroinfiltration. These fusion proteins, under the control of the CaMV 35S promoter, were expressed in rice callus cells,and the localization of the fusion protein was determined by visualization with a fluorescence microscope. The result indicates that WR1–GFP was localized primarily in the nucleus, while GFP-only was observed in cytosol.
 
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Real-time PCR (Q-PCR) amplifications showed that the
 
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expression of OsWR1 was quickly responded at 1 h, and
 
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then gradually increased to peak at 2.5 h after drought
 
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treatment. The transcripts of OsWR1 induced by ABA
 
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treatment were clearly observed at 6 h, and reached max-
 
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imum induction at 9 h and then decreased, whereas the
 
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induction of OsWR1 by salt treatment was dramatically
 
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increased at 3 h and reached a maximum at 6 h, and sustained high induction thereafter.
 
  
 
===Evolution===
 
===Evolution===
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One of the rice WIN1-like genes, OsWR1(Os02g0202000), encodes a protein of 205 amino acids with a predicted protein molecular weight of 22.45 kDa.
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* The ERF family is a large gene family of transcription factors and is part of the AP2/ERF superfamily, which also contains the AP2 and RAV families
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The amino acid of full length OsWR1 shows a high similarity to WIN1/SHN1 (68.8%), SHN2 (58%), SHN3(57.8%) and OsWR2 (Os06g0604000, 81%), but low
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* The AP2/ERF superfamily is defined by the AP2/ERF domain, which consists of about 60 to 70 amino acids and is involved in DNA binding.
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similarity to OsWR3 (Os02g0797100, 41.4%).these all contain the conserved ERF domain , suggesting that OsWR1 is a homology of WIN1/SHN1.
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* The AP2 family proteins contain two repeated AP2/ERF domains, the ERF family proteins contain a single AP2/ERF domain, and the RAV family proteins contain a B3 domain, which is a DNA-binding domain conserved in other plant-specific transcription factors, including VP1/ABI3, in addition to the single AP2/ERF domain.
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* The expansion of the ERF family in plants might have been due to chromosomal/segmental duplication and tandem duplication, as well as more ancient transposition and homing.
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* Since rice is a cultivated species, selection either during domestication from its wild ancestor or during agricultural improvement in the subsequent time may also have been important for the evolution of rice ERF family.<ref name="ref3" />
  
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===References===
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You can also add sub-section(s) at will.
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Youhua Wang;Liyun Wan;Lixia Zhang;Zhijin Zhang;Haiwen Zhang;Ruidang Quan;Shirong Zhou;Rongfeng Huang
 
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An ethylene response factor OsWR1 responsive to drought stress transcriptionally activates wax synthesis related genes and increases wax production in rice
 
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Plant Molecular Biology, 2012, 78(3): 275-288
 
  
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Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A (2004)
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==Labs working on this gene==
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The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis. Plant Cell 16:2463–2480
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* Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305–8566, Japan (T.N., K.S., H.S.);
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* Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305–8672, Japan (T.N., T.F.)
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Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL (2004)
 
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WIN1, a transcriptional activator of epidermal wax accumulation
 
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in Arabidopsis. Proc Natl Acad Sci USA 101:4706–4711
 
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Jung KH, Han MJ, Lee DY, Lee YS, Schreiber L, Franke R, Faust A,
 
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Yephremov A, Saedler H, Kim YW, Hwang I, An G (2006)
 
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Wax-deficient anther1 is involved in cuticle and wax production
 
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in rice anther walls and is required for pollen development. Plant
 
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Cell 18:3015–3032
 
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Rowland O, Zheng H, Hepworth SR, Lam P, Jetter R, Kunst L (2006)
 
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CER4 encodes an alcohol-forming fatty acyl-coenzyme a
 
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reductase involved in cuticular wax production in Arabidopsis.
 
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Plant Physiol 142:866–877
 
  
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==Labs working on this gene==
 
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Biotechnology Research Institute, Chinese Academy
 
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of Agricultural Sciences, Beijing 100081, China
 
  
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National Key Facility of Crop Gene Resources and Genetic
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==References==
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Improvement, Beijing 100081, China
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<references>
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* <ref name="ref1">
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Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF
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gene family in Arabidopsis and rice. Plant Physiol. 2006 Feb;140(2):411-32.
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PubMed PMID: 16407444; PubMed Central PMCID: PMC1361313.
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</ref>
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* <ref name="ref2">
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Chuck G, Muszynski M, Kellogg E, Hake S, Schmidt RJ. The control of spikelet
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meristem identity by the branched silkless1 gene in maize. Science. 2002 Nov
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8;298(5596):1238-41. PubMed PMID: 12424380.
 +
</ref>
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* <ref name="ref3">
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Riechmann JL, Meyerowitz EM. The AP2/EREBP family of plant transcription
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factors. Biol Chem. 1998 Jun;379(6):633-46. Review. PubMed PMID: 9687012.
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</ref>
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</references>
  
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National Center for Plant Gene Research (Beijing),
 
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Beijing 100081, China
 
  
 
==Structured Information==
 
==Structured Information==

Revision as of 08:04, 23 March 2017

The rice Os06g0181700 was reported as OsERF#002 in 2006 [1] by researchers from Japan.

Annotated Information

Gene Symbol

  • Os06g0181700 <=> OsERF#002

Function

  • Genes in the ERF family encode transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants.
  • It has been demonstrated that the AP2/ERF proteins have important functions in the transcriptional regulation of a variety of biological processes related to growth and development, as well as various responses to environmental stimuli.
  • Genes in the AP2 family have been shown to participate in the regulation of developmental processes, e.g. flower development (Elliott et al., 1996), spikelet meristem determinacy (Chuck et al., 1998), leaf epidermal cell identity (Moose and Sisco, 1996), and embryo development (Boutilier et al., 2002).[2]

Evolution

  • The ERF family is a large gene family of transcription factors and is part of the AP2/ERF superfamily, which also contains the AP2 and RAV families
  • The AP2/ERF superfamily is defined by the AP2/ERF domain, which consists of about 60 to 70 amino acids and is involved in DNA binding.
  • The AP2 family proteins contain two repeated AP2/ERF domains, the ERF family proteins contain a single AP2/ERF domain, and the RAV family proteins contain a B3 domain, which is a DNA-binding domain conserved in other plant-specific transcription factors, including VP1/ABI3, in addition to the single AP2/ERF domain.
  • The expansion of the ERF family in plants might have been due to chromosomal/segmental duplication and tandem duplication, as well as more ancient transposition and homing.
  • Since rice is a cultivated species, selection either during domestication from its wild ancestor or during agricultural improvement in the subsequent time may also have been important for the evolution of rice ERF family.[3]

You can also add sub-section(s) at will.

Labs working on this gene

  • Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305–8566, Japan (T.N., K.S., H.S.);
  • Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305–8672, Japan (T.N., T.F.)


References

  1. ↑ Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 2006 Feb;140(2):411-32. PubMed PMID: 16407444; PubMed Central PMCID: PMC1361313.
  2. ↑ Chuck G, Muszynski M, Kellogg E, Hake S, Schmidt RJ. The control of spikelet meristem identity by the branched silkless1 gene in maize. Science. 2002 Nov 8;298(5596):1238-41. PubMed PMID: 12424380.
  3. ↑ Riechmann JL, Meyerowitz EM. The AP2/EREBP family of plant transcription factors. Biol Chem. 1998 Jun;379(6):633-46. Review. PubMed PMID: 9687012.


Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0181700&oldid=277327"