Difference between revisions of "Os01g0177400"

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Please input one-sentence summary here.
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The rice '''''Os01g0177400''''' was reported as '''''OsGA3ox2''''' in 2003 <ref name="ref1" /> by researchers from Japan.  
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os01g0177400''''' '''''<=>''''' '''''GA3OX2, GA3ox1,  OsGA3ox2, d18,OsD18'''''
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===Function===
 
===Function===
Please input function information here.
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* OsGA3ox2 (D18) encodes GA 3-oxidase.
D18 CDS 1113 bp, contains three exons, encoding a 370 amino acid composition of protein products.
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* OsGA3ox2 is important in controlling bioactive GA synthesis for shoot elongation but not for reproductive development.
Phytochrome in the regulation of rice section elongation of different levels, such as regulating OsGA3ox2 GA biosynthesis gene expression, the expression of ACO1 section and the elongation of the initial (Iwamoto et al. 2011)
 
Gibberellin 3 beta hydroxylase activity (0016707) GO:
 
  
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===Phenotypic analysis===
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The rice d18 mutant is a severe GA-deficient dwarf, but its flower and grain development are not affected。
 
===Expression===
 
===Expression===
Please input expression information here.
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* In wild-type rice, expression of OsGA3ox2 is regulated by the endogenous bioactive GA content.  
Rice genome library makes use of degenerate primers PCR amplification, filter and cloned into two gibberellin 3 beta OsGA3ox1 and OsGA3ox2 hydroxylase gene.The authors use the GC - MS full range scan and Kovats retention index to test the function of the recombinant fusion protein.Two proteins in GA20 to GA1, GA5 to GA3 and GA44 to GA38 and GA9 to GA4 show hydroxylase activity.In addition, the indirect evidence also suggests that proteins in catalytic GA9 OsGA3ox1 to 2, 3 - dehydro - GA9, GA20 to GA5 (2, 3 - dehydro - GA20) have 2, 3 dehydrogenase activity, in the process of the catalytic GA1 to GA8, GA4 to GA34 plays in the process of 2 beta hydroxylase activity.Molecules and linkage analysis showed that OsGA3ox1 located in chromosome 5 short arm distal, OsGA3ox2 located in chromosome 1 short arm distal D18 seat.OsGA3ox2 and verified by complementary experiments, the correlation of d18 by OsGA3ox2 complementary d18 - AD alleles, gm strains showed normal phenotype.The two genes are characterized by brief expression, including OsGA3ox1 expressed in unopened flower, highest OsGA3ox2 expressed in elongation of blade top (Itoh et al., 2001)
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* Indeed, the OsGA3ox2 transcript was upregulated by uniconazole (an inhibitor of GA biosynthesis) treatment and downregulated by bioactive GA 3 application —as was the transcript of the green revolution rice gene, OsGA20ox2 (SD1), which encodes GA 20-oxidase 9 (20ox2 in Fig. 2b).
 
 
===Evolution===
 
Please input evolution information here.
 
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
===Extension===  
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===Knowledge Extension===  
Its number Genebank is AB056519 , and a 1122 bp    mRNA .
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* Dwarfing is one of the most valuable traits in crop breeding, because it results in plants that are more resistant to damage by wind and rain (lodging resistant) and have stable increased yields.
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* Dwarf mutants have been extensively characterized in many plant species. The endogenous phytohormone GA is one of several factors associated with the dwarf phenotype.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
Please input related labs here.
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* Field Production Science Center, The University of Tokyo, 1-1-1 Midoricyo, Nishi-Tokyo 188-0002, Japan.
1. Makoto Matsuoka
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* National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.  
personal home page:http://www.bio.nagoya-u.ac.jp/gcoe/english/member/matsuoka.html
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* BioResource Center, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.  
2. Makoto Takano
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* Bioscience and Biotechnology Center, Nagoya University, Furocyo, Chikusa, Nagoya, Aichi 464-8601, Japan.  
personal home page:http://www.pimco.com/EN/experts/pages/makototakano.aspx
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* Institute of Agriculture and Forestry, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
  
 
==References==
 
==References==
Please input cited references here.
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<references>
1. Masao Iwamoto;Seiichiro Kiyota;Atsushi Hanada;Shinjiro Yamaguchi;Makoto Takano
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* <ref name="ref1">
  The Multiple Contributions of Phytochromes to the Control of Internode Elongation in Rice
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Sakamoto T, Morinaka Y, Ishiyama K, Kobayashi M, Itoh H, Kayano T, Iwahori S,
  Plant Physiology, 2011, 157(3): 1187-1195
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Matsuoka M, Tanaka H. Genetic manipulation of gibberellin metabolism in
2. Hironori Itoh;Miyako Ueguchi-Tanaka;Naoki Sentoku;Hidemi Kitano;Makoto Matsuoka;and Masatomo Kobayashi
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transgenic rice. Nat Biotechnol. 2003 Aug;21(8):909-13. Epub 2003 Jul 13. PubMed
  Cloning and functional analysis of two gibberellin 3β-hydroxylase genes that are differently expressed during the growth of rice
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PMID: 12858182.
  Proceedings of the National Academy of Sciences, 2001, 98(15): 8909-8914
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</ref>
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</references>
  
  

Revision as of 08:50, 8 May 2017

The rice Os01g0177400 was reported as OsGA3ox2 in 2003 [1] by researchers from Japan.

Annotated Information

Gene Symbol

  • Os01g0177400 <=> GA3OX2, GA3ox1, OsGA3ox2, d18,OsD18

Function

  • OsGA3ox2 (D18) encodes GA 3-oxidase.
  • OsGA3ox2 is important in controlling bioactive GA synthesis for shoot elongation but not for reproductive development.

Phenotypic analysis

The rice d18 mutant is a severe GA-deficient dwarf, but its flower and grain development are not affected。

Expression

  • In wild-type rice, expression of OsGA3ox2 is regulated by the endogenous bioactive GA content.
  • Indeed, the OsGA3ox2 transcript was upregulated by uniconazole (an inhibitor of GA biosynthesis) treatment and downregulated by bioactive GA 3 application —as was the transcript of the green revolution rice gene, OsGA20ox2 (SD1), which encodes GA 20-oxidase 9 (20ox2 in Fig. 2b).

You can also add sub-section(s) at will.

Knowledge Extension

  • Dwarfing is one of the most valuable traits in crop breeding, because it results in plants that are more resistant to damage by wind and rain (lodging resistant) and have stable increased yields.
  • Dwarf mutants have been extensively characterized in many plant species. The endogenous phytohormone GA is one of several factors associated with the dwarf phenotype.

Labs working on this gene

  • Field Production Science Center, The University of Tokyo, 1-1-1 Midoricyo, Nishi-Tokyo 188-0002, Japan.
  • National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
  • BioResource Center, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
  • Bioscience and Biotechnology Center, Nagoya University, Furocyo, Chikusa, Nagoya, Aichi 464-8601, Japan.
  • Institute of Agriculture and Forestry, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.

References

  1. Sakamoto T, Morinaka Y, Ishiyama K, Kobayashi M, Itoh H, Kayano T, Iwahori S, Matsuoka M, Tanaka H. Genetic manipulation of gibberellin metabolism in transgenic rice. Nat Biotechnol. 2003 Aug;21(8):909-13. Epub 2003 Jul 13. PubMed PMID: 12858182.