Difference between revisions of "Os06g0493100"

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The rice '''''Os06g0493100''''' was reported as '''''Bphi008a''''' in 2011 <ref name="ref1" /> by researchers from China.  
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os06g0493100''''' '''''<=>''''' ''''' BpHi008A, OsRALF-22, OsRALF22, RALF-22, RALF22, bphi008a'''''
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===Function===
 
===Function===
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The ''Brown planthopper induced008a'' (''Bphi008a''; AY256682) gene of rice (''Oryza sativa'') enhances the plant’s resistance to the brown planthopper (BPH; ''Nilaparvata lugens''). BPH feeding rapidly initiated the ethylene (ET) signaling pathway and up-regulated ''Bphi008a'' transcript levels after 6 to 96 h of feeding. In contrast, blocking ethylene transduction (using 1-methylcyclopropene) reduced ''Bphi008a'' transcript levels in wild-type plants fed upon by BPH. Because ''Bphi008a'' is a downstream gene of the Et signaling pathway in rice. The overexpressed (OE) plants had greater resistance to the BPH than wildtype plants (Fig. 1wD). In contrast, the change in susceptibility of rice to the BPH caused by RNAi of ''Bphi008a'' was not obvious. The level of ''Bphi008a'' expression affects BPH feeding on the phloem of rice (Fig. 1wE). The ''Bphi008a'' expression enhances rice resistance to the BPH by impairing BPH feeding (Fig.1wF). The ''Bphi008a'' can be phosphorylated by rice Mitogen-activated Protein Kinase5 (OsMPK5). Yeast two-hybrid assays demonstrated that the carboxyl-terminal proline-rich region of ''Bphi008a'' interacts directly with this kinase, and this interaction occurs in the nucleus. Subsequently, we found that ''Bphi008a'' up-regulation and down-regulation were accompanied by different changes in transcription levels of OsMPK5, OsMPK12, OsMPK13, and OsMPK17 in transgenic plants (Fig. 2w). Immunoblot analysis also showed that the OsMPK5 protein level increased in overexpressing plants and decreased in RNA interference plants after BPH feeding(Fig. 3w, A and B). The ''Bphi008a'' Overexpression Enhances Levels of AP2/ERF Transcription Factors and Defense-Related Genes. Yeast two-hybrid screening results showed that a clear interaction between ''Bphi008a''and a b-ZIP transcription factor (OsbZIP60), but a weak interaction between  ''Bphi008a'' and a RNA polymerase polypeptide (SDRP) (Fig. 4wB). Furthermore, analysis of OsbZIP60 expression levels in wild-type and transgenic plants after BPH feeding from 0 to 96 h (Fig. 4wD) indicated that ''Bphi008a'' (possibly phosphorylated) might form a transcriptional complex with OsbZIP60 and SDRP in vivo that activates the transcription of target genes.<ref name="ref1" />
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* The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding. <ref name="ref1" />
  
 
===Expression===
 
===Expression===
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In a previous study, ''Bphi008a'' expression was found to be induced by both BPH feeding and spraying plants with ethephon, which slowly releases Et.<ref name="ref2" />.In rice, a large gene family controls Et biosynthesis, composed of six 1-aminocyclopropane-1-carboxylic acid (ACC) synthase genes (designated OsACS1–OsACS6) and
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* In a previous study, ''Bphi008a'' expression was found to be induced by both BPH feeding and spraying plants with ethephon, which slowly releases Et <ref name="ref2" />.
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seven ACC oxidase genes (designated OsACO1–OsACO7)<ref name="ref3" /><ref name="ref4" />. Experimental results indicate that Bphi008a transcription is stimulated by active Et(Fig. 5w).Bphi008a expression levels were highest in the roots of seedlings, followed (in order) by leaf blades and leaf sheaths at the heading stage, then leaf sheaths, leaf blades, and stems of seedlings. The lowest detected level of Bphi008a expression was in the flowers (Fig. 6w).
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* Measurement of the expression levels of ethylene synthases and of ethylene emissions showed that BPH feeding rapidly initiated the ethylene signaling pathway and up-regulated Bphi008a transcript levels after 6 to 96 h of feeding. In contrast, blocking ethylene transduction (using 1-methylcyclopropene) reduced Bphi008a transcript levels in wild-type plants fed upon by BPH.<ref name="ref2" />.<ref name="ref4" />.<ref name="ref3" />.
  
 
===Evolution===
 
===Evolution===
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Please input evolution information here.
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* In transgenic lines, changes in the expression levels of several enzymes that are important components of the defenses against the BPH were also observed.
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* Yeast two-hybrid screening results showed that Bphi008a is able to interact with a b-ZIP transcription
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factor (OsbZIP60) and a RNA polymerase polypeptide (SDRP).
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, China.
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* State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People’s Republic of China
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* College of Life Sciences, Xinyang Normal University, Xinyang 464000, People’s Republic of China
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College of Life Sciences, Xinyang Normal University, China.
 
  
 
==References==
 
==References==

Revision as of 12:52, 8 May 2017

The rice Os06g0493100 was reported as Bphi008a in 2011 [1] by researchers from China.

Annotated Information

Gene Symbol

  • Os06g0493100 <=> BpHi008A, OsRALF-22, OsRALF22, RALF-22, RALF22, bphi008a

Function

  • The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding. [1]

Expression

  • In a previous study, Bphi008a expression was found to be induced by both BPH feeding and spraying plants with ethephon, which slowly releases Et [2].
  • Measurement of the expression levels of ethylene synthases and of ethylene emissions showed that BPH feeding rapidly initiated the ethylene signaling pathway and up-regulated Bphi008a transcript levels after 6 to 96 h of feeding. In contrast, blocking ethylene transduction (using 1-methylcyclopropene) reduced Bphi008a transcript levels in wild-type plants fed upon by BPH.[2].[3].[4].

Evolution

  • In transgenic lines, changes in the expression levels of several enzymes that are important components of the defenses against the BPH were also observed.
  • Yeast two-hybrid screening results showed that Bphi008a is able to interact with a b-ZIP transcription

factor (OsbZIP60) and a RNA polymerase polypeptide (SDRP).

You can also add sub-section(s) at will.

Labs working on this gene

  • State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People’s Republic of China
  • College of Life Sciences, Xinyang Normal University, Xinyang 464000, People’s Republic of China

References

  1. ↑ 1.0 1.1 Jing Hu, Jiangbo Zhou, Xinxin Peng, Henghao Xu, Caixiang Liu, Bo Du, Hongyu Yuan, Lili Zhu, and Guangcun He. The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding1[C][W][OA][J].Plant Physiology, 2011, 156: 856-872.
  2. ↑ 2.0 2.1 Yuan H, Chen X, Zhu L, He G. Isolation and characterization of a novel rice gene encoding a putative insect-inducible protein homologous to wheat Wir1[J].Plant Physiol, 2004, 161: 79-85.
  3. ↑ Iwai T, Miyasaka A, Seo S, Ohashi Y. Contribution of ethylene biosynthesis for resistance to blast fungus infection in young rice plants[J]. Plant Physiol, 2006, 142: 1202-1215.
  4. ↑ Yang SF, Hoffman NE. Ethylene biosynthesis and its regulation in higher plants[J]. Annu Rev Plant Physiol, 1984, 35: 155-189.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0493100&oldid=277962"