Os11g0249000
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Contents
Annotated Information
Introduction
One of the main defense mechanisms is resistance (R) gene-mediated disease resistance. The majority of R genes characterized encode proteins that have a central nucleotide-binding region (NB) plus a C- terminal leucine-rich repeat (LRR).The NB-LRR proteins recognize the corresponding avirulence (Avr) effectors produced by the pathogen and activate defense responses.
Function
NLS1 displays spontaneous lesions, specifically on leaf sheaths, with a developmental pattern. nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes, and enhanced resistance to bacterial pathogens.
Expression
NLS1 encodes a typical CC-NB-LRR-type protein in rice and causes a S367N substitution in the non-conserved region close to the GLPL motif of the NB domain
Evolution
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Summary
In this study, we characterized the semi-dominant mutant nls1-1D (necrotic leaf sheath 1) of rice, which displays spontaneous lesions, specifically on leaf sheaths, with a developmental pattern. nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes, and enhanced resistance to bacterial pathogens. Map-based cloning revealed that NLS1 encodes a typical CC-NB-LRR-type protein in rice. The nls1-1D mutation causes a S367N substitution in the non-conserved region close to the GLPL motif of the NB domain. An adjacent S366T substitution was found in another semi-dominant mutant, nls1-2D, which exhibited the same phenotypes as nls1-1D. Combined analyses of wild-type plants transformed with the mutant NLS1 gene (nls1-1D), NLS1 RNAi and over-expression transgenic lines showed that nls1-2D is allelic to nls1-1D, and both mutations may cause constitutive auto-activation of the NLS1 R protein. Further real-time PCR analysis revealed that NLS1 is expressed constitutively in an age-dependent manner. In addition, because the morphology and constitutive defense responses of nls1-1D were not suppressed by blocking SA or NPR1 transcript accumulation, we suggest that NLS1 mediates both SA and NPR1-independent defense signaling pathways in rice.
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os11g0249000 |
|---|---|
| Description |
Disease resistance protein family protein |
| Version |
NM_001074150.1 GI:115484948 GeneID:4350204 |
| Length |
5550 bp |
| Definition |
Oryza sativa Japonica Group Os11g0249000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 11:8074465..8080014 |
| Sequence Coding Region |
8074504..8075291,8077897..8079865 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008404:8074465..8080014 source=RiceChromosome11 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008404:8074465..8080014 source=RiceChromosome11 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggaagcagcgatagtgagtgcttcgacgggagtgatgcgttccctcctcgccaagctcacaaaccttcttggtggtgagtacaagctacttaagtggctccgtagggagatggagttcctcgagagcgagctccgtagcatgagcatcttcttggagaggttggaggacacgcaaaagcttcacccccaaatgaaggattggagggatcgagtacgcgagttggcatacgacattgaggattgcatcgacgacttcatactacagcttgattccaaggatgcgaaggttgggtttgggcaaaagcttcttgcatcccgtaggattggccacatgattcgagaactcaaggcgcgtgtaatggaggagagtgagagacaaaggaggtacatgcttgatggcttggcatctgggcctagtgttcgtgtgaaggttgatccacggctatcggctctctatgtcgacgaggacagattagttggtattgatgccccgagagatgagatcattgggcggctgttggacaagcggagaagtgcctcagcaaaacaggtcatgacaatttccatcgtgggttgcggaggattggggaagaccacccttgccaatcaaatttactgcaaaatcaaaggaaaatttgagtgcgcagcttttgcgtcagtgttccaaaatcccaacaccaagaaggttttgacgaatattctttcccaagtggccactactgctgctgtggaagacgatgagcaagccatcatcaacaagctaagggaataccttagtgacaagaggtacattgtcatagttgatgatatatgggatatgcaaatatggaaatttattgaatgtgcgttggtcaagaactgccgtggtagcagaattatcacaacaacacgtatacatgatattgctaaattgtgctgttcatctcatggcgattatatttacgaaatgaagcctcttggagttattgactccaaaatattgttcgacaaaagaatttttgatccagaggaaaggcgcccaccgcagttgacagaagtttctgaggaaatattgaaaaaatgtggtggtttgcctttggcgatcatttccatatcaagtttgttggcaagcaaaccaaagtcaaaagatcaatgggacagggtcaaggtttctcttagctctacgcttgaaaggactccagacattgaaaccatggaatgggtgctatcactcagttactctgatcttcctaatcatttaaaaacatgtctgttatatttgagtatattccctgagggctatgagataaacagggaacgtttggtgagtagatggattgctgaaggattcatttacaaaaaacatggacaaaatccatatgaagtgggggacagttattttaatgagcttgtgaacagaagcttgattcaaccagcaaacattaagcctgatggtcagacaaatgcttgtcgagtggatgacaccgttcatgattttattgtgtccatgtcagttgaagaaaatttcgttacattatttggtggttcaaaattggtaccaaggtcacatggtaaggttcgtcgattatctattcaaaatggcggcattcaagaaaatattgtcacatcaacgcacttggttacatcgcaagtccggtcactaactttatttgctgtagaaatgccatctcttttaggttttggtatgttacgtgtattggacctagaagattgttatgcattagaagaccatcatcttacgaacctggaaaggctagtccagttacggtatttgagtataagaacatcacccattagtgaactcccgaaacaaattgggcagcttcaatatctggaaacactagatttaagagctacgggtgtggaagaattgccatcaaccattggtagactaaagagtttggtccgtttattcgttgattatcatgttaaattaccaaaagaaatcagcaatatgcatgcactggaagagctaacaagtttctcagccttgatgtactctccagactttctgaaagaactaggtcagcttacaaacatgagggtgctccgagtaatttgtgactgtgatagctttaaaggtgatgcaggaagttgcttggaaaatttagcttcttctttatgtaatctaggcacatataaccttcattccctttttgttgacatcaatggatatggtgaagacaacttctcactagatacatggcaacctgttccatctagactaagaagattttccattgaccggtggtgccccatcaataagattccgaattgggtgggttcactgatcaaccttgaagagttagtcctatatgtcaacaaaatttggcaggaggatttcgaattgctaggacatatgcctgctctgtcatctctgaccatatactcaaatactgcccttcaagggaggatcatcatcagtggattccattctaccaagtttttcaagttctattgcaatcctgcagggttaacctttgacgcaggaagtctgttaaagctcgaatgccttgatgtcataatgaatgtgttcaacacgaagtcttcaaatggtagctttgattttggcattcaatatcttaccaaccttagaaatgtctacattcaacttgattgtaatgggtcgactggtggagaattggaagcagctaaagcttccatcaagagctcagtaaacaaacttccaggacagcctaagctgaacttgagcacattgaatgaaaatatgttagtccatgaagaagctaattaa</cdnaseq> |
| Protein Sequence |
<aaseq>MEAAIVSASTGVMRSLLAKLTNLLGGEYKLLKWLRREMEFLESE LRSMSIFLERLEDTQKLHPQMKDWRDRVRELAYDIEDCIDDFILQLDSKDAKVGFGQK LLASRRIGHMIRELKARVMEESERQRRYMLDGLASGPSVRVKVDPRLSALYVDEDRLV GIDAPRDEIIGRLLDKRRSASAKQVMTISIVGCGGLGKTTLANQIYCKIKGKFECAAF ASVFQNPNTKKVLTNILSQVATTAAVEDDEQAIINKLREYLSDKRYIVIVDDIWDMQI WKFIECALVKNCRGSRIITTTRIHDIAKLCCSSHGDYIYEMKPLGVIDSKILFDKRIF DPEERRPPQLTEVSEEILKKCGGLPLAIISISSLLASKPKSKDQWDRVKVSLSSTLER TPDIETMEWVLSLSYSDLPNHLKTCLLYLSIFPEGYEINRERLVSRWIAEGFIYKKHG QNPYEVGDSYFNELVNRSLIQPANIKPDGQTNACRVDDTVHDFIVSMSVEENFVTLFG GSKLVPRSHGKVRRLSIQNGGIQENIVTSTHLVTSQVRSLTLFAVEMPSLLGFGMLRV LDLEDCYALEDHHLTNLERLVQLRYLSIRTSPISELPKQIGQLQYLETLDLRATGVEE LPSTIGRLKSLVRLFVDYHVKLPKEISNMHALEELTSFSALMYSPDFLKELGQLTNMR VLRVICDCDSFKGDAGSCLENLASSLCNLGTYNLHSLFVDINGYGEDNFSLDTWQPVP SRLRRFSIDRWCPINKIPNWVGSLINLEELVLYVNKIWQEDFELLGHMPALSSLTIYS NTALQGRIIISGFHSTKFFKFYCNPAGLTFDAGSLLKLECLDVIMNVFNTKSSNGSFD FGIQYLTNLRNVYIQLDCNGSTGGELEAAKASIKSSVNKLPGQPKLNLSTLNENMLVH EEAN</aaseq> |
| Gene Sequence |
<dnaseqindica>40..827#3433..5401#acaacatgtgctgtgaatttgaaaggggaaataccagctatggaagcagcgatagtgagtgcttcgacgggagtgatgcgttccctcctcgccaagctcacaaaccttcttggtggtgagtacaagctacttaagtggctccgtagggagatggagttcctcgagagcgagctccgtagcatgagcatcttcttggagaggttggaggacacgcaaaagcttcacccccaaatgaaggattggagggatcgagtacgcgagttggcatacgacattgaggattgcatcgacgacttcatactacagcttgattccaaggatgcgaaggttgggtttgggcaaaagcttcttgcatcccgtaggattggccacatgattcgagaactcaaggcgcgtgtaatggaggagagtgagagacaaaggaggtacatgcttgatggcttggcatctgggcctagtgttcgtgtgaaggttgatccacggctatcggctctctatgtcgacgaggacagattagttggtattgatgccccgagagatgagatcattgggcggctgttggacaagcggagaagtgcctcagcaaaacaggtcatgacaatttccatcgtgggttgcggaggattggggaagaccacccttgccaatcaaatttactgcaaaatcaaaggaaaatttgagtgcgcagcttttgcgtcagtgttccaaaatcccaacaccaagaaggttttgacgaatattctttcccaagtggccactactgctgctgtggaagacgatgagcaagccatcatcaacaagctaagggaataccttagtgacaagaggtacgtacaccaccatccctatgtttttttttatgacgttttaatatggattaggatctaatactccctctatttttactttggccaagtttacagagaaattttagcaacatctataacaacaaatttgttttattaaaactaacatacaatatatttgataatatgtttgttttgtgttgaacatgttactatattttttataaacctagtcaaacataaaaaaaaattaactaacaaaagagtcaaaacgattaatgatatgaaacatatgtagtaattgatattcctagtataccatgtagggtactacactcaagggtaatatagacttttcgctaatcctatacacctcacatgctacaaatgctgatctaatatggtacttctccacactccttgtaagtggcacaggtgctccgtagcaattgagacctgccaataatccttccatttatccttatatgcgtgaacccgccatgggcattcatctttctccttcacacacacctcacctcataaacatttttgcatgacttctctaccctcaactccctttgaatcttccaatggaaaaagtacgaattacacccccccccccgaactatcgcggttgaccgaattaccccctacccgattgagatgacttaatctctatcgggcattaactaaagaccatcacagatgactcatccgtgacggactctatactaatgcccgattgagatgacttaatctttatcgggcattaactaaagcccatcactgatgattatttttccattttccatatgaaatgtttatattcaatttatgtggtattttttattttcaatttatgagaactttaaatataattttaggctgatttttgtgtttcaaatgtaaaaaacacaaacaacaaaattgtacaactcatcgagatttacaactattgtattggtcctttttacaaataagtacatttgaacaatttaaattttgaatgttcaaaatttgacatcttcgaacaaaaatagggttaataaatgatatcaaatagaaaagtcaccaaaaccaaaattgtagaattcatcgaggtgcaaaatttttattttggtcatttttccatctgactctatttgaacaatttgaaatttgaaattcaaagtttgagaactttaaatagaattctttttattagtgaacgacttcaactgaaaagtcattaacaacaacaaagctgtataactcatcaaggtctataacttttattttggtcatttttctatataacgttttttaacaatttgaatttgaatttgaaaatatgacaacttcaaataacatttttaaatactaaatgatttcaactagaaaagtcatcaacaacaaagttgtataacacatcaagatctataacttttatttcggtcatttctttattcgacaaagtgatagtaacattattcacaaaattcacatatctctcatctagctattttatgaacaatgttactaacactttgtcagatgaaaaaatgacaaaaataaaagttatagatcttgataagttatacaactttgttgttgatgacttttttagttgaaattatttagtatttgaaaatgttgttttgaagttatcatattttcaaattcaaattcaaactattcaaaaaaagttatatagaaaaatggccaaactaaaagttgtaaatcttgataagttatacaattttgttgttgacaattttttcatttggaatcatttagtatttgaaaatgttatttgaagttgtcatatttttaaattcaaattcaaactgttaaaaaaagttatatagaaaaatgaccaaactaaaagttgtaaatcttgataagttatacaattttgttgttgacaatttttccatttgaaatcatttactacccgaaaaattattttgactaataaaataaattattatactgcagttaattattttgctacaggtcaacttacaatataaacatttcatataaaaaatagaaaaatactcatcggtgatgggcattagttaaaccctgatagagattaagtcatatcaatcgagcattagtatagagcccgtcatatcaatcgggcctagtttgtatctaaatcgggcccaagttggtgcccgtcacggatgacagccaacttgaggcccgattgagatatggaatgttatctcaatcaggcctaaactacggcctgtcacgaatagattttattcgtgacgggcctgagttttatacccatctaacatgtctgtgcgaccatatctcaatcggacgctttatcggcccgattgagataacttccttgtgacagcccgctaattccaggcatgttagaggcagccacagatggaaggatctgtacttgtgtcagataagcataaaaatttcgtctagctccattgctcatgctccctttttactttttttttagtttttttgtcactcattttaatataagaattaatagttatttgagatttattttcctatttacatgtaataattaaggtagtacatggttattcctcagaaaactgtaactccgtgcgtgcataactcaaacatgtgatgtgttacacaggtacattgtcatagttgatgatatatgggatatgcaaatatggaaatttattgaatgtgcgttggtcaagaactgccgtggtagcagaattatcacaacaacacgtatacatgatattgctaaattgtgctgttcatctcatggcgattatatttacgaaatgaagcctcttggagttattgactccaaaatattgttcgacaaaagaatttttgatccagaggaaaggcgcccaccgcagttgacagaagtttctgaggaaatattgaaaaaatgtggtggtttgcctttggcgatcatttccatatcaagtttgttggcaagcaaaccaaagtcaaaagatcaatgggacagggtcaaggtttctcttagctctacgcttgaaaggactccagacattgaaaccatggaatgggtgctatcactcagttactctgatcttcctaatcatttaaaaacatgtctgttatatttgagtatattccctgagggctatgagataaacagggaacgtttggtgagtagatggattgctgaaggattcatttacaaaaaacatggacaaaatccatatgaagtgggggacagttattttaatgagcttgtgaacagaagcttgattcaaccagcaaacattaagcctgatggtcagacaaatgcttgtcgagtggatgacaccgttcatgattttattgtgtccatgtcagttgaagaaaatttcgttacattatttggtggttcaaaattggtaccaaggtcacatggtaaggttcgtcgattatctattcaaaatggcggcattcaagaaaatattgtcacatcaacgcacttggttacatcgcaagtccggtcactaactttatttgctgtagaaatgccatctcttttaggttttggtatgttacgtgtattggacctagaagattgttatgcattagaagaccatcatcttacgaacctggaaaggctagtccagttacggtatttgagtataagaacatcacccattagtgaactcccgaaacaaattgggcagcttcaatatctggaaacactagatttaagagctacgggtgtggaagaattgccatcaaccattggtagactaaagagtttggtccgtttattcgttgattatcatgttaaattaccaaaagaaatcagcaatatgcatgcactggaagagctaacaagtttctcagccttgatgtactctccagactttctgaaagaactaggtcagcttacaaacatgagggtgctccgagtaatttgtgactgtgatagctttaaaggtgatgcaggaagttgcttggaaaatttagcttcttctttatgtaatctaggcacatataaccttcattccctttttgttgacatcaatggatatggtgaagacaacttctcactagatacatggcaacctgttccatctagactaagaagattttccattgaccggtggtgccccatcaataagattccgaattgggtgggttcactgatcaaccttgaagagttagtcctatatgtcaacaaaatttggcaggaggatttcgaattgctaggacatatgcctgctctgtcatctctgaccatatactcaaatactgcccttcaagggaggatcatcatcagtggattccattctaccaagtttttcaagttctattgcaatcctgcagggttaacctttgacgcaggaagtctgttaaagctcgaatgccttgatgtcataatgaatgtgttcaacacgaagtcttcaaatggtagctttgattttggcattcaatatcttaccaaccttagaaatgtctacattcaacttgattgtaatgggtcgactggtggagaattggaagcagctaaagcttccatcaagagctcagtaaacaaacttccaggacagcctaagctgaacttgagcacattgaatgaaaatatgttagtccatgaagaagctaattaattaaatggaaggggacgtaaatttcgagtacgaacattgcaggtatcaatttcggttttgattttttttttttgcagaaaggaccagtaccaaaaataccatttgtaatttatagtctaacaattctttacccatcaaaataattctcc</dnaseqindica> 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