Os11g0249000
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Contents
Annotated Information
Introduction
One of the main defense mechanisms is resistance (R) gene-mediated disease resistance. The majority of R genes characterized encode proteins that have a central nucleotide-binding region (NB) plus a C- terminal leucine-rich repeat (LRR).The NB-LRR proteins recognize the corresponding avirulence (Avr) effectors produced by the pathogen and activate defense responses. In most cases, these defense responses are accompanied by a hypersensitivity response (HR) in the infected cells, triggering the rapid production of reactive oxygen species (ROS).These defense responses are also associated with accumulation of salicylic acid (SA), which functions in the induction of numerous pathogenesis-related (PR) genes, and the establishment of systemic acquired resistance (SAR).SA is required in R gene-mediated defense signaling pathways. It is assumed that R proteins are present in an auto-inhibited state and are unable to activate defense responses in the absence of pathogens. Here, we describe the semi-dominant rice mutant nls1-1D ( necrotic leaf sheath 1 ), which displayed tissue-specific and age-dependent spontaneous necrotic lesions and defense-related phenotypes.
Keywords: NLS1, CC-NB-LRR, disease resistance protein, lesion, salicylic acid, rice.
Function
NLS1 displays spontaneous lesions, specifically on leaf sheaths, with a developmental pattern. nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes, and enhanced resistance to bacterial pathogens.NLS1 encodes a typical CC-NB-LRR-type protein in rice and causes a S367N substitution in the non-conserved region close to the GLPL motif of the NB domain
Expression
NLS1 encodes a typical CC-NB-LRR-type protein in rice and causes a S367N substitution in the non-conserved region close to the GLPL motif of the NB domain
Evolution
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Summary
In this study, we characterized the semi-dominant mutant nls1-1D (necrotic leaf sheath 1) of rice, which displays spontaneous lesions, specifically on leaf sheaths, with a developmental pattern. nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes, and enhanced resistance to bacterial pathogens. Map-based cloning revealed that NLS1 encodes a typical CC-NB-LRR-type protein in rice. The nls1-1D mutation causes a S367N substitution in the non-conserved region close to the GLPL motif of the NB domain. An adjacent S366T substitution was found in another semi-dominant mutant, nls1-2D, which exhibited the same phenotypes as nls1-1D. Combined analyses of wild-type plants transformed with the mutant NLS1 gene (nls1-1D), NLS1 RNAi and over-expression transgenic lines showed that nls1-2D is allelic to nls1-1D, and both mutations may cause constitutive auto-activation of the NLS1 R protein. Further real-time PCR analysis revealed that NLS1 is expressed constitutively in an age-dependent manner. In addition, because the morphology and constitutive defense responses of nls1-1D were not suppressed by blocking SA or NPR1 transcript accumulation, we suggest that NLS1 mediates both SA and NPR1-independent defense signaling pathways in rice.
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os11g0249000 |
|---|---|
| Description |
Disease resistance protein family protein |
| Version |
NM_001074150.1 GI:115484948 GeneID:4350204 |
| Length |
5550 bp |
| Definition |
Oryza sativa Japonica Group Os11g0249000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 11:8074465..8080014 |
| Sequence Coding Region |
8074504..8075291,8077897..8079865 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008404:8074465..8080014 source=RiceChromosome11 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008404:8074465..8080014 source=RiceChromosome11 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggaagcagcgatagtgagtgcttcgacgggagtgatgcgttccctcctcgccaagctcacaaaccttcttggtggtgagtacaagctacttaagtggctccgtagggagatggagttcctcgagagcgagctccgtagcatgagcatcttcttggagaggttggaggacacgcaaaagcttcacccccaaatgaaggattggagggatcgagtacgcgagttggcatacgacattgaggattgcatcgacgacttcatactacagcttgattccaaggatgcgaaggttgggtttgggcaaaagcttcttgcatcccgtaggattggccacatgattcgagaactcaaggcgcgtgtaatggaggagagtgagagacaaaggaggtacatgcttgatggcttggcatctgggcctagtgttcgtgtgaaggttgatccacggctatcggctctctatgtcgacgaggacagattagttggtattgatgccccgagagatgagatcattgggcggctgttggacaagcggagaagtgcctcagcaaaacaggtcatgacaatttccatcgtgggttgcggaggattggggaagaccacccttgccaatcaaatttactgcaaaatcaaaggaaaatttgagtgcgcagcttttgcgtcagtgttccaaaatcccaacaccaagaaggttttgacgaatattctttcccaagtggccactactgctgctgtggaagacgatgagcaagccatcatcaacaagctaagggaataccttagtgacaagaggtacattgtcatagttgatgatatatgggatatgcaaatatggaaatttattgaatgtgcgttggtcaagaactgccgtggtagcagaattatcacaacaacacgtatacatgatattgctaaattgtgctgttcatctcatggcgattatatttacgaaatgaagcctcttggagttattgactccaaaatattgttcgacaaaagaatttttgatccagaggaaaggcgcccaccgcagttgacagaagtttctgaggaaatattgaaaaaatgtggtggtttgcctttggcgatcatttccatatcaagtttgttggcaagcaaaccaaagtcaaaagatcaatgggacagggtcaaggtttctcttagctctacgcttgaaaggactccagacattgaaaccatggaatgggtgctatcactcagttactctgatcttcctaatcatttaaaaacatgtctgttatatttgagtatattccctgagggctatgagataaacagggaacgtttggtgagtagatggattgctgaaggattcatttacaaaaaacatggacaaaatccatatgaagtgggggacagttattttaatgagcttgtgaacagaagcttgattcaaccagcaaacattaagcctgatggtcagacaaatgcttgtcgagtggatgacaccgttcatgattttattgtgtccatgtcagttgaagaaaatttcgttacattatttggtggttcaaaattggtaccaaggtcacatggtaaggttcgtcgattatctattcaaaatggcggcattcaagaaaatattgtcacatcaacgcacttggttacatcgcaagtccggtcactaactttatttgctgtagaaatgccatctcttttaggttttggtatgttacgtgtattggacctagaagattgttatgcattagaagaccatcatcttacgaacctggaaaggctagtccagttacggtatttgagtataagaacatcacccattagtgaactcccgaaacaaattgggcagcttcaatatctggaaacactagatttaagagctacgggtgtggaagaattgccatcaaccattggtagactaaagagtttggtccgtttattcgttgattatcatgttaaattaccaaaagaaatcagcaatatgcatgcactggaagagctaacaagtttctcagccttgatgtactctccagactttctgaaagaactaggtcagcttacaaacatgagggtgctccgagtaatttgtgactgtgatagctttaaaggtgatgcaggaagttgcttggaaaatttagcttcttctttatgtaatctaggcacatataaccttcattccctttttgttgacatcaatggatatggtgaagacaacttctcactagatacatggcaacctgttccatctagactaagaagattttccattgaccggtggtgccccatcaataagattccgaattgggtgggttcactgatcaaccttgaagagttagtcctatatgtcaacaaaatttggcaggaggatttcgaattgctaggacatatgcctgctctgtcatctctgaccatatactcaaatactgcccttcaagggaggatcatcatcagtggattccattctaccaagtttttcaagttctattgcaatcctgcagggttaacctttgacgcaggaagtctgttaaagctcgaatgccttgatgtcataatgaatgtgttcaacacgaagtcttcaaatggtagctttgattttggcattcaatatcttaccaaccttagaaatgtctacattcaacttgattgtaatgggtcgactggtggagaattggaagcagctaaagcttccatcaagagctcagtaaacaaacttccaggacagcctaagctgaacttgagcacattgaatgaaaatatgttagtccatgaagaagctaattaa</cdnaseq> |
| Protein Sequence |
<aaseq>MEAAIVSASTGVMRSLLAKLTNLLGGEYKLLKWLRREMEFLESE LRSMSIFLERLEDTQKLHPQMKDWRDRVRELAYDIEDCIDDFILQLDSKDAKVGFGQK LLASRRIGHMIRELKARVMEESERQRRYMLDGLASGPSVRVKVDPRLSALYVDEDRLV GIDAPRDEIIGRLLDKRRSASAKQVMTISIVGCGGLGKTTLANQIYCKIKGKFECAAF ASVFQNPNTKKVLTNILSQVATTAAVEDDEQAIINKLREYLSDKRYIVIVDDIWDMQI WKFIECALVKNCRGSRIITTTRIHDIAKLCCSSHGDYIYEMKPLGVIDSKILFDKRIF DPEERRPPQLTEVSEEILKKCGGLPLAIISISSLLASKPKSKDQWDRVKVSLSSTLER TPDIETMEWVLSLSYSDLPNHLKTCLLYLSIFPEGYEINRERLVSRWIAEGFIYKKHG QNPYEVGDSYFNELVNRSLIQPANIKPDGQTNACRVDDTVHDFIVSMSVEENFVTLFG GSKLVPRSHGKVRRLSIQNGGIQENIVTSTHLVTSQVRSLTLFAVEMPSLLGFGMLRV LDLEDCYALEDHHLTNLERLVQLRYLSIRTSPISELPKQIGQLQYLETLDLRATGVEE LPSTIGRLKSLVRLFVDYHVKLPKEISNMHALEELTSFSALMYSPDFLKELGQLTNMR VLRVICDCDSFKGDAGSCLENLASSLCNLGTYNLHSLFVDINGYGEDNFSLDTWQPVP SRLRRFSIDRWCPINKIPNWVGSLINLEELVLYVNKIWQEDFELLGHMPALSSLTIYS NTALQGRIIISGFHSTKFFKFYCNPAGLTFDAGSLLKLECLDVIMNVFNTKSSNGSFD FGIQYLTNLRNVYIQLDCNGSTGGELEAAKASIKSSVNKLPGQPKLNLSTLNENMLVH EEAN</aaseq> |
| Gene Sequence |
<dnaseqindica>40..827#3433..5401#acaacatgtgctgtgaatttgaaaggggaaataccagctatggaagcagcgatagtgagtgcttcgacgggagtgatgcgttccctcctcgccaagctcacaaaccttcttggtggtgagtacaagctacttaagtggctccgtagggagatggagttcctcgagagcgagctccgtagcatgagcatcttcttggagaggttggaggacacgcaaaagcttcacccccaaatgaaggattggagggatcgagtacgcgagttggcatacgacattgaggattgcatcgacgacttcatactacagcttgattccaaggatgcgaaggttgggtttgggcaaaagcttcttgcatcccgtaggattggccacatgattcgagaactcaaggcgcgtgtaatggaggagagtgagagacaaaggaggtacatgcttgatggcttggcatctgggcctagtgttcgtgtgaaggttgatccacggctatcggctctctatgtcgacgaggacagattagttggtattgatgccccgagagatgagatcattgggcggctgttggacaagcggagaagtgcctcagcaaaacaggtcatgacaatttccatcgtgggttgcggaggattggggaagaccacccttgccaatcaaatttactgcaaaatcaaaggaaaatttgagtgcgcagcttttgcgtcagtgttccaaaatcccaacaccaagaaggttttgacgaatattctttcccaagtggccactactgctgctgtggaagacgatgagcaagccatcatcaacaagctaagggaataccttagtgacaagaggtacgtacaccaccatccctatgtttttttttatgacgttttaatatggattaggatctaatactccctctatttttactttggccaagtttacagagaaattttagcaacatctataacaacaaatttgttttattaaaactaacatacaatatatttgataatatgtttgttttgtgttgaacatgttactatattttttataaacctagtcaaacataaaaaaaaattaactaacaaaagagtcaaaacgattaatgatatgaaacatatgtagtaattgatattcctagtataccatgtagggtactacactcaagggtaatatagacttttcgctaatcctatacacctcacatgctacaaatgctgatctaatatggtacttctccacactccttgtaagtggcacaggtgctccgtagcaattgagacctgccaataatccttccatttatccttatatgcgtgaacccgccatgggcattcatctttctccttcacacacacctcacctcataaacatttttgcatgacttctctaccctcaactccctttgaatcttccaatggaaaaagtacgaattacacccccccccccgaactatcgcggttgaccgaattaccccctacccgattgagatgacttaatctctatcgggcattaactaaagaccatcacagatgactcatccgtgacggactctatactaatgcccgattgagatgacttaatctttatcgggcattaactaaagcccatcactgatgattatttttccattttccatatgaaatgtttatattcaatttatgtggtattttttattttcaatttatgagaactttaaatataattttaggctgatttttgtgtttcaaatgtaaaaaacacaaacaacaaaattgtacaactcatcgagatttacaactattgtattggtcctttttacaaataagtacatttgaacaatttaaattttgaatgttcaaaatttgacatcttcgaacaaaaatagggttaataaatgatatcaaatagaaaagtcaccaaaaccaaaattgtagaattcatcgaggtgcaaaatttttattttggtcatttttccatctgactctatttgaacaatttgaaatttgaaattcaaagtttgagaactttaaatagaattctttttattagtgaacgacttcaactgaaaagtcattaacaacaacaaagctgtataactcatcaaggtctataacttttattttggtcatttttctatataacgttttttaacaatttgaatttgaatttgaaaatatgacaacttcaaataacatttttaaatactaaatgatttcaactagaaaagtcatcaacaacaaagttgtataacacatcaagatctataacttttatttcggtcatttctttattcgacaaagtgatagtaacattattcacaaaattcacatatctctcatctagctattttatgaacaatgttactaacactttgtcagatgaaaaaatgacaaaaataaaagttatagatcttgataagttatacaactttgttgttgatgacttttttagttgaaattatttagtatttgaaaatgttgttttgaagttatcatattttcaaattcaaattcaaactattcaaaaaaagttatatagaaaaatggccaaactaaaagttgtaaatcttgataagttatacaattttgttgttgacaattttttcatttggaatcatttagtatttgaaaatgttatttgaagttgtcatatttttaaattcaaattcaaactgttaaaaaaagttatatagaaaaatgaccaaactaaaagttgtaaatcttgataagttatacaattttgttgttgacaatttttccatttgaaatcatttactacccgaaaaattattttgactaataaaataaattattatactgcagttaattattttgctacaggtcaacttacaatataaacatttcatataaaaaatagaaaaatactcatcggtgatgggcattagttaaaccctgatagagattaagtcatatcaatcgagcattagtatagagcccgtcatatcaatcgggcctagtttgtatctaaatcgggcccaagttggtgcccgtcacggatgacagccaacttgaggcccgattgagatatggaatgttatctcaatcaggcctaaactacggcctgtcacgaatagattttattcgtgacgggcctgagttttatacccatctaacatgtctgtgcgaccatatctcaatcggacgctttatcggcccgattgagataacttccttgtgacagcccgctaattccaggcatgttagaggcagccacagatggaaggatctgtacttgtgtcagataagcataaaaatttcgtctagctccattgctcatgctccctttttactttttttttagtttttttgtcactcattttaatataagaattaatagttatttgagatttattttcctatttacatgtaataattaaggtagtacatggttattcctcagaaaactgtaactccgtgcgtgcataactcaaacatgtgatgtgttacacaggtacattgtcatagttgatgatatatgggatatgcaaatatggaaatttattgaatgtgcgttggtcaagaactgccgtggtagcagaattatcacaacaacacgtatacatgatattgctaaattgtgctgttcatctcatggcgattatatttacgaaatgaagcctcttggagttattgactccaaaatattgttcgacaaaagaatttttgatccagaggaaaggcgcccaccgcagttgacagaagtttctgaggaaatattgaaaaaatgtggtggtttgcctttggcgatcatttccatatcaagtttgttggcaagcaaaccaaagtcaaaagatcaatgggacagggtcaaggtttctcttagctctacgcttgaaaggactccagacattgaaaccatggaatgggtgctatcactcagttactctgatcttcctaatcatttaaaaacatgtctgttatatttgagtatattccctgagggctatgagataaacagggaacgtttggtgagtagatggattgctgaaggattcatttacaaaaaacatggacaaaatccatatgaagtgggggacagttattttaatgagcttgtgaacagaagcttgattcaaccagcaaacattaagcctgatggtcagacaaatgcttgtcgagtggatgacaccgttcatgattttattgtgtccatgtcagttgaagaaaatttcgttacattatttggtggttcaaaattggtaccaaggtcacatggtaaggttcgtcgattatctattcaaaatggcggcattcaagaaaatattgtcacatcaacgcacttggttacatcgcaagtccggtcactaactttatttgctgtagaaatgccatctcttttaggttttggtatgttacgtgtattggacctagaagattgttatgcattagaagaccatcatcttacgaacctggaaaggctagtccagttacggtatttgagtataagaacatcacccattagtgaactcccgaaacaaattgggcagcttcaatatctggaaacactagatttaagagctacgggtgtggaagaattgccatcaaccattggtagactaaagagtttggtccgtttattcgttgattatcatgttaaattaccaaaagaaatcagcaatatgcatgcactggaagagctaacaagtttctcagccttgatgtactctccagactttctgaaagaactaggtcagcttacaaacatgagggtgctccgagtaatttgtgactgtgatagctttaaaggtgatgcaggaagttgcttggaaaatttagcttcttctttatgtaatctaggcacatataaccttcattccctttttgttgacatcaatggatatggtgaagacaacttctcactagatacatggcaacctgttccatctagactaagaagattttccattgaccggtggtgccccatcaataagattccgaattgggtgggttcactgatcaaccttgaagagttagtcctatatgtcaacaaaatttggcaggaggatttcgaattgctaggacatatgcctgctctgtcatctctgaccatatactcaaatactgcccttcaagggaggatcatcatcagtggattccattctaccaagtttttcaagttctattgcaatcctgcagggttaacctttgacgcaggaagtctgttaaagctcgaatgccttgatgtcataatgaatgtgttcaacacgaagtcttcaaatggtagctttgattttggcattcaatatcttaccaaccttagaaatgtctacattcaacttgattgtaatgggtcgactggtggagaattggaagcagctaaagcttccatcaagagctcagtaaacaaacttccaggacagcctaagctgaacttgagcacattgaatgaaaatatgttagtccatgaagaagctaattaattaaatggaaggggacgtaaatttcgagtacgaacattgcaggtatcaatttcggttttgattttttttttttgcagaaaggaccagtaccaaaaataccatttgtaatttatagtctaacaattctttacccatcaaaataattctcc</dnaseqindica> 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