Os03g0811100
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Contents
Annotation information
Photosynthetic organisms exhibit a green color due to the accumulation of chlorophyll pigments in chloroplasts. Mg-protoporphyrin IX chelatase (Mgchelatase) comprises three subunits (ChlH, ChlD and ChlI) and catalyzes the insertion of Mg2+ into protoporphyrin IX, the last common intermediate precursor in both chlorophyll and heme biosyntheses, to produce Mg-protoporphyrin IX (MgProto). Chlorophyll deficiency in higher plants results in chlorina (yellowishgreen) phenotype. The grana are poorly stacked, resulting in the underdevelopment of chloroplasts. In the seedlings fed with aminolevulinate-dipyridyl in darkness, MgProto levels in the chl1 and chl9 mutants decreased up to 25% and 31% of that in wildtype, respectively, indicating that the Mg-chelatase activity is significantly reduced, causing the eventual decrease in chlorophyll synthesis. Furthermore, Northern blot analysis indicated that the nuclear genes encoding the three subunits of Mg-chelatase and LhcpII in chl1 mutant are expressed about 2-fold higher than those inWT, but are not altered in the chl9 mutant. This result indicates that the ChlD subunit participates in negative feedback regulation of plastidto- nucleus in the expression of nuclear genes encoding chloroplast proteins, but not the ChlI subunit.
Function
Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development.MgProto accumulated at a lowerlevel in both chl1 and chl9 mutants when the seedlings were fed by ALA-DP in complete darkness, indicating that Mg-chelatase activity is severely reduced, resulting in decrease in chlorophyll synthesis and underdevelopment of chloroplats. In addition, the ChlD subunit of Mg-chelatase also negatively regulates the expression of nuclear genes encoding photosynthetic proteins in chloroplasts. Chl1 and Chl9 genes encode the OsChlD and OsChlI subunits of Mg-chelatase, respectively, that play an important role in chloroplast development as a modulator of MgProto. Northern blots analysis revealed that ChlD subunit participates in the negative feedback regulation for the expression of nuclear genes encoding chloroplast proteins.
Expression
Rice Chl1 and Chl9 genes on chromosome 3 encode ChlD and ChlI,in chl1 mutants, the levels of OsChlH and OsChlI transcripts increased about 2.5-fold and OsChlD transcript levels were about 1.7-fold higher than the wild-type. In the chl9 mutant, however, OsChlH and OsChlD transcript levels were not altered and the OsChlI transcript was also present in the mutant.expression levels of photosynthetic genes such as the nuclear LhcpII genes encoding a Chl-binding thylakoid protein and the chloroplast RbcL gene encoding a stroma protein to determine if the expressions of nuclear and chloroplast genes were affected in the chl1 and chl9 mutants. Functional OsChlD subunit encoded by the Chl1 gene participates in the negative feedback regulation of plastid-to-nucleus signaling mechanisms that control the expression levels of nuclear genes encoding chloroplast proteins. The expression of chloroplast genes like RbcL is not affected by the activity of Mg-chelatase.
Evolution
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Labs working on this gene
Department of Plant Science, Seoul National University
References
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Structured Information
| Gene Name |
Os03g0811100 |
|---|---|
| Description |
Similar to Magnesium-chelatase subunit chlD, chloroplast precursor (EC 6.6.1.1) (Mg-protoporphyrin IX chelatase) (Mg-chelatase subunit D) |
| Version |
NM_001058203.1 GI:115456134 GeneID:4334537 |
| Length |
6670 bp |
| Definition |
Oryza sativa Japonica Group Os03g0811100, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 3:34806026..34812695 |
| Sequence Coding Region |
34806050..34806310,34807137..34807315,34807489..34807573,34807653..34807969,34808130..34808253 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008396:34806026..34812695 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008396:34806026..34812695 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcgatggccaccaccgcgctctccgcctccctcccgcgcctactcccgcctcgccgccgccgcttcccgacgccctcctcctcctccccctccgccgcatccacctccacctcccgcgtcgtccgcctgcgggccgccgcggcctcggcgccatccgaggtcctcgactccaccaacggggccatcccctcggggaagggcggcggcgggcagcagtacgggagggagtatttccccttggccgctgtcgtcgggcaggatgcaattaaaactgctctgctgcttggggcaattgaccgtgaaattggaggcatcgccatctcagggaagcgtgggacagcaaagacagtgatggctcgtggcttgcacgctatgcttccacctattgaagtggttgtaggctcgattgcaaatgctgaccctaactacccagaagaatgggaggagggtttggctaaccaagttcaatatgatgctgatggtaacttgaagaccgagattatcaaaacaccttttgtgcagatcccgcttggtatcactgaggataggttaatcggatcagtcgatgttgaagcatctgtgaaatcaggaactactgtgtttcaacctggccttcttgctgaagctcacagaggcgttctttatgttgatgagataaatctattggatgagggcgtaagcaatctacttctgaatgtcttgactgagggagtcaatattgtggaaagagagggcattagctttcgtcatccatgcaaaccacttctaattgctacttacaacccagaggaaggatctgtacgtgaacacttacttgatcgtattgcaattaatttaagcgctgatctgccaatgagttttgatgatcgtgtggcagctgtggatattgcaacacaatttcaagagtccagcaaagaggtttttaaaatggtggaagaagaaactgaggttgcaaaaacccagataattttggcaagagaatatctgaaagatgttgcaatcagcacagagcagctcaaatatcttgtcatggaagctatacgcggtggctgtcaggggcaccgggctgagctgtatgctgctcgagtggcaaaatgtcttgctgctatggaagggcgtgaaaaagtatatgtggatgaccttaagaaagctgtagagctagttattctacctcgatcaatcctatctgataacccacaggagcagcaagaccaacaacctcctccacccccaccgccaccccctccacaagatcaagattctcaagaagatcaagatgaagacgaggaagaggaccaagaggacgatgatgaagaaaatgaacagcaggaccagcagatacctgaggagttcatttttgatgctgaaggtggtatagtagatgagaagctccttttctttgctcagcaagctcaaagacggcgagggaaagctggacgagcaaagaatctcatattctcatctgataggggacgatacataggttctatgcttcccaagggtccaataaggaggttagctgttgatgccacacttcgagcagctgcaccataccagaaactgaggagagagaaagatcgtgacaagacaagaaaggtttttgttgaaaaaactgacatgagagccaaaagaatggctcgaaaagcaggcgcactggtcatatttgttgtggatgctagcggtagcatggctctgaatcgcatgcagaatgcgaaaggtgcagcattaaagttgcttgcagaaagctacacaagcagagatcaggtttcaatcattccatttcgtggagattttgctgaggttcttcttccaccttcaagatccatagcaatggcccgcaatcgtcttgagaagctaccatgtggtggcggttctcctttagctcacggccttagcacagctgtcagagtgggtttgaatgctgaaaagagcggtgatgttggacgtatcatgattgttgcaatcaccgatggaagagctaatgtgtcactgaagaaatcgactgacccagaagccacttcagatgctccaagaccttcttctcaagaattaaaggatgagatacttgaggtggctggcaaaatatacaaggctggaatttcacttcttgttattgataccgagaacaagtttgtatccacaggatttgccaaggaaattgcaagggtcgcccaaggtaaatactattacctgccgaatgcttcagacgctgttatttccgccgccaccaagactgcactctcggacctgaagagttcgtga</cdnaseq> |
| Protein Sequence |
<aaseq>MAMATTALSASLPRLLPPRRRRFPTPSSSSPSAASTSTSRVVRL RAAAASAPSEVLDSTNGAIPSGKGGGGQQYGREYFPLAAVVGQDAIKTALLLGAIDRE IGGIAISGKRGTAKTVMARGLHAMLPPIEVVVGSIANADPNYPEEWEEGLANQVQYDA DGNLKTEIIKTPFVQIPLGITEDRLIGSVDVEASVKSGTTVFQPGLLAEAHRGVLYVD EINLLDEGVSNLLLNVLTEGVNIVEREGISFRHPCKPLLIATYNPEEGSVREHLLDRI AINLSADLPMSFDDRVAAVDIATQFQESSKEVFKMVEEETEVAKTQIILAREYLKDVA ISTEQLKYLVMEAIRGGCQGHRAELYAARVAKCLAAMEGREKVYVDDLKKAVELVILP RSILSDNPQEQQDQQPPPPPPPPPPQDQDSQEDQDEDEEEDQEDDDEENEQQDQQIPE EFIFDAEGGIVDEKLLFFAQQAQRRRGKAGRAKNLIFSSDRGRYIGSMLPKGPIRRLA VDATLRAAAPYQKLRREKDRDKTRKVFVEKTDMRAKRMARKAGALVIFVVDASGSMAL NRMQNAKGAALKLLAESYTSRDQVSIIPFRGDFAEVLLPPSRSIAMARNRLEKLPCGG GSPLAHGLSTAVRVGLNAEKSGDVGRIMIVAITDGRANVSLKKSTDPEATSDAPRPSS QELKDEILEVAGKIYKAGISLLVIDTENKFVSTGFAKEIARVAQGKYYYLPNASDAVI SAATKTALSDLKSS</aaseq> |
| Gene Sequence |
<dnaseqindica>25..285#1112..1290#1464..1548#1628..1944#2105..2228#2932..3024#3418..3513#3827..3976#4050..4247#4535..4690#4838..4945#5114..5248#5331..5486#5666..5786#6304..6389#accgcgctctctccctcccctcccatggcgatggccaccaccgcgctctccgcctccctcccgcgcctactcccgcctcgccgccgccgcttcccgacgccctcctcctcctccccctccgccgcatccacctccacctcccgcgtcgtccgcctgcgggccgccgcggcctcggcgccatccgaggtcctcgactccaccaacggggccatcccctcggggaagggcggcggcgggcagcagtacgggagggagtatttccccttggccgctgtcgtcgggcaggtgagtgagcgagcaactctctgcatagcttctcctgcattggtgttgctgaagacacaagtaccaactaccaagtagtgtggagtggtacaagtaatttgattccccagttgagttgatctgatgcatttcgttgcgcggtatcgcgaatgctgttacaatagagattcgaaatccaacagatgcatttcggtttttgtcgtgttttatgctaatgctattgtaagcgaatcaatcagtggacaaatagtgatcaccgatcagtacacaatgtgccaatgtacatctctgaaaaaatgcctttgctatgagttattttgctgtcatactctctctgaagatgaaacaaacgagctggaatgactttcaagctttagttttcctcccattgttccctgcatcgagttatttttagataatggaagctttattaatatactcagtcaattacacaaagacaatacaaccgcatcgggcccaccccggcctctgcatatgatgcacacagccaaaagaaccaaaaacatccttcccaagcacaaaagaataaccaaaacatagggattagtggctatcaatccgtagactagatcgccacccatgctcctggataaaaaaactccgtgtcacctggtccaactatcgtgatactaccgcaatagtatcccgaagtcccggtttgtggaggacagcctaggtacgtagccaccgggtgactaatgtaataacctgcaaaggagaagacaccatcttgttatcaaaccattgatttatggtctcaactttgtatatgttacaattttaacttaccttattgtgcgtccaggatgcaattaaaactgctctgctgcttggggcaattgaccgtgaaattggaggcatcgccatctcagggaagcgtgggacagcaaagacagtgatggctcgtggcttgcacgctatgcttccacctattgaagtggttgtaggctcgattgcaaatgctgaccctaactacccagaagagtaatgtccccaaatgcatgtctaataatgcttctagaacttctgtttaagcatccaaattcttcttttcacccccacaaagttttacataccaactacaatttgaatatgtttactttcatttctgaaatgattgtcaggtaactcacttgattgttctcctcttatggcagatgggaggagggtttggctaaccaagttcaatatgatgctgatggtaacttgaagaccgagattatcaaaacaccttttgtgcaggtatgctgcatggactttttgtttaaaaaataatttcgacataccattgttagttcaatttcaggtttccccttgacagatcccgcttggtatcactgaggataggttaatcggatcagtcgatgttgaagcatctgtgaaatcaggaactactgtgtttcaacctggccttcttgctgaagctcacagaggcgttctttatgttgatgagataaatctattggatgagggcgtaagcaatctacttctgaatgtcttgactgagggagtcaatattgtggaaagagagggcattagctttcgtcatccatgcaaaccacttctaattgctacttacaacccagaggaaggatctgtacgtgaacacttacttgatcgtattgcaattaatttaaggtactttagactatggatcactcatttactcacttcttttaaccaagttcaggttgtattatcttatacaccctctgttggtgtggagaacataagaaaactctcaatgcatttatgaaataccacagattgctcattccatattgttgttttgttgcagcgctgatctgccaatgagttttgatgatcgtgtggcagctgtggatattgcaacacaatttcaagagtccagcaaagaggtttttaaaatggtggaagaagaaactgaggttgcaaaaacccaggtaccaccgatactgttaaactagggatgtaaacctgtaagatagagtacctcctttattgaactagaatagggtgtagaaactatttagcttttgcttttttttttgtattgcacacaaatagctttcttcagaacagcgatatctaatcaatcagtatcacaaaatatgtggattcattaatatgcttcataaacaaatggtcacatcagacaagttgatacattgatttttggatctttctcacctaattacaacttgtgccatttacatatgatttttcccttgccttcatagtcgaaagtatatacgctattcatggttctgtattttgagatcccaacgtcatccactcatgctgaattaactgcatgtacccacatttacattatattccctgtgttgttctgccacttaacatattttatctggtccaacattcaaaacatcaatctttaatacgtataaaaggtatatgatggcacacaaaataatgccattctatttttgttggacaatactttcacagtttatgacttctattattttactagtatgtagtattgaaaaaataacgtggccaaagtcccacattgaagactatggaaaatcatgagcgactttaactagcaacgatggaggtttagttgaaaacaatattatgcactgttcatctcacaggtggtaatacatggtctaacagataattttggcaagagaatatctgaaagatgttgcaatcagcacagagcagctcaaatatcttgtcatggaagctatacgcggtggctgtcaggttagtttattttgcagtgaaagaattttttagctctgtttggcttaagctgagtaaggagcttgattggaatattgcactgtccttgtaatggtctgtagtctgtaccccatgatcatgcattctggtcataacatgtggcgaggtggctaaaaactgtctgctgtaaccattgatttcattttagttgtgtttatcatgccctgaaatcgatgtggctttgcattattttctgcttgtctagatattatacctgtccgctgtgcaccatgtggttaatgtaatacctgaagcaatcatggtttttgagacactgatgctacttttttgtggttaatgtaattcaaaaagtggctgtgttctcatgtaaataaaactgacactcatttgcaggggcaccgggctgagctgtatgctgctcgagtggcaaaatgtcttgctgctatggaagggcgtgaaaaagtatatgtggatgaccttaagaaagctgttaagtatacttatgcctttgaattcttcattatttaatacagtaagacaccccctgtaatttcctgtttacaactattccttttcatctctttactgaaatgtaccgaaggaaacatgcatcatgatttgttgttaagaacaaaatagaaagctgcaaacttgcacgtaataaaagtcacagcaaaataaattttgccctcgcatggctcacaattttcttctgccatgctggctgtttgcattaatgtaatgatgtcagtatattctgaataaagttaagacatttgacatgtgtgccaccttcttgcaggtagagctagttattctacctcgatcaatcctatctgataacccacaggagcagcaagaccaacaacctcctccacccccaccgccaccccctccacaagatcaagattctcaagaagatcaagatgaagacgaggaagaggaccaagaggttaatacatataccatttaactaacttattcattttgttttctcgtagctcattagctgtactcttggacaggacgatgatgaagaaaatgaacagcaggaccagcagatacctgaggagttcatttttgatgctgaaggtggtatagtagatgagaagctccttttctttgctcagcaagctcaaagacggcgagggaaagctggacgagcaaagaatctcatattctcatctgataggggacgatacataggttctatgcttcccaaggtaaacttcttaatcactttattgtaccaagattcctgaatccagatccttgtttcctctcaattcaatgcctgtagtgttttagagtttctcctatctagaaatgtgtgcataatcattttgcctattttcctctgatgctttctgctgtgttctgtcatttactgatatactatcaaacaatagtcatatactgctgtgttgccttgtattctgccgttcccatcctattgcttggcacctttatcacaagtaggctccaatttgaacagttaattgtgttgtagggtccaataaggaggttagctgttgatgccacacttcgagcagctgcaccataccagaaactgaggagagagaaagatcgtgacaagacaagaaaggtttttgttgaaaaaactgacatgagagccaaaagaatggctcgaaaagcaggcgcactggtaaaaatctctctctctctctctctctctctctctctccaagcctctgagattatcagaatatctgtttgctctgtccttgttatgaagtaaacattgttgtccgatcataagagttaactgctctgtggaattacattttgacaggtcatatttgttgtggatgctagcggtagcatggctctgaatcgcatgcagaatgcgaaaggtgcagcattaaagttgcttgcagaaagctacacaagcagagatcaggtactagttgttaccctgtacgccaccttcagttgttcttccccttgcccctggagatgtgtctttttttctttcaatgtgtaaaaggtatatggttatatggctagcttgtatgctatcgatttttatactggccaatcttaccttatattttgtcatctatctcaggtttcaatcattccatttcgtggagattttgctgaggttcttcttccaccttcaagatccatagcaatggcccgcaatcgtcttgagaagctaccatgtggtggcggttctcctttagctcacggccttagcacagtaagaactaagcattttctcgaacctagctatatgttacagcccatatgtattgattaacaattcggtttttacctcaaaggctgtcagagtgggtttgaatgctgaaaagagcggtgatgttggacgtatcatgattgttgcaatcaccgatggaagagctaatgtgtcactgaagaaatcgactgacccagaagccacttcagatgctccaagaccttcttctcaagaattaaaggtacaactcgtatctaatatcacatggtagtaatgatatgaaaagtatttatttaaactggaaacctgtgaccatcactctgaagctgatatgatgtacgtaaactacttccaagttgcattcttaaaagcatgtatcaatcactctaaagctgatacgatatatttatcttttccaaggatgagatacttgaggtggctggcaaaatatacaaggctggaatttcacttcttgttattgataccgagaacaagtttgtatccacaggatttgccaaggaaattgcaagggtcgcccaaggtactctcggcgtgatatctcttgaattagtagccttcgtttcaaatataagttgttatagatttgcacacatgaattaagaaaatagcttgaataactttgttgcccttcgtttatactacattggaaaaaaagggatctatttatttatcagaggggtactatcgatcttgtgaggacaaacatggaagaaataaaaaaacacgcagaagtttcaaaccaacatatatttagaaaagagttgaaaaaactaaaatgacctacatttggaattagaggcatatacaccatctgccgtaggaataccattatctttcttttctgcatcttattacaccattacttatgagttaagatcataagtctaatttgccacctatttttgactagcaaccttgtaatgttgccatcgcacaaagagatatcttccatgtacatcaatcactaagcaccaaaatactgccaaatgtttgtgcccaatctcctgatataaacctctctttctcgatctctacaggtaaatactattacctgccgaatgcttcagacgctgttatttccgccgccaccaagactgcactctcggacctgaagagttcgtgatcctggagagcgttttaccttcagataatgagtggtttttaccttttaccttgtttggtgcagcagtgtccatgtttcgtgtaactttgggacgtttcggctgtgataaccaattttggcataggatttttaccgtgagagttggaattcgggcgtagcaccgtgtaaagaatcatataatccctcttctgtctaaataattggccatgtaaatatggtgttattgcgtacagttctaagtaataataacattcataatttatgtgaagaaagaaattgcc</dnaseqindica> 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