Os01g0578500
SaF+/SaF- is an important gene impact the fertility in hybrids between indica and japonica subspecies of Asian cultivated rice(Oryza sativa).
Contents
Annotated Information
Function
Sterility is common in hybrids between divergent populations,such as the indica and japonica subspecies of Asian cultivated rice(Oryza sativa).SaF is one gene of the Sa locus,which include two adjacent genes SaF and SaM.SaM encoding a small ubiquitin-like modifier E3 ligase-like protein and an F-box protein. Most indica cultivars contain a haplotype SaM+SaF+,whereas all japonica cultivars have SaM-SaF- that diverged by nucleotide variations in wild rice. Male semi-sterility in this heterozygous complex locus is caused by abortion of pollen carrying SaM-. This allele-specific gamete elimination results from a selective interaction of SaF+ with SaM-, a truncated protein, but not with SaM+ because of the presence of an inhibitory domain, although SaM+ is required for this male sterility. Lack of any one of the three alleles in recombinant plants does not produce male sterility.
SaF Interacts with SaM-� but not with SaM+�. A bacterial two-hybrid(B2H) interaction assay demonstrated that both SaF+� and SaF�- interacted physically with SaM�- but not with SaM+�.Therefore, the amino acid substitution in SaF-� did not affect its physical interaction with SaM-� but impaired the biological function of SaF-� for male sterility.There is a self-inhibitory domain within the 203–218 region of SaM�+ blocks the interaction, probably by affecting the protein’s structure.
Functional analysis of SaM+,SaM-,and SaF+ in rice by transformation indicate that the segregation distortion of the Sa region is a consequence of hybrid male sterility caused by the allele-specific gamete selection.
Expression
SaF encodes a 476-aa protein with an F-box and a plantspecific F-box protein domain (FBD). F-box proteins mediate protein–protein interactions, but the function of the FBD is unclear. The SaF protein is homologous to a ribosomal RNA apurinic site-specific lyase (RALyase) in wheat (23) with 33% identity in the overall sequences and 64% and 44% identities in the F-box and FBD, respectively (Fig. 2C). About 20 members of the F-box/FBD subfamily are predicted in rice, and they have up to 75% identity to RALyase and up to 40% identity to SaF.In the coding region of SaF, only one SNP was found between SaF-and SaF�+, which results in a Phe-to-Ser substitution in position 287 (Fig. 2 A and B). SaF was expressed in all tissues tested as examined by RT-PCR, albeit at different levels (Fig. 3A). This analysis showed that only the cDNA of SaF� was detectable in the purified pollen of the F1 plants, providing molecular evidence for the selective abortion of SaM�-carrying pollen.
Evolution
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Structured Information
| Gene Name |
Os01g0578500 |
|---|---|
| Description |
Cyclin-like F-box domain containing protein |
| Version |
NM_001185502.1 GI:297720138 GeneID:9266343 |
| Length |
1733 bp |
| Definition |
Oryza sativa Japonica Group Os01g0578500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 1:24032420..24034152 |
| Sequence Coding Region |
24032420..24033334,24033421..24033564,24033781..24034152 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008394:24032420..24034152 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008394:24032420..24034152 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggaggcggtcatcgcggagtgccgccccaagcctctcttcaccaccgggcccttcctctccgccgtcggcggcggcggcggcggcgtcgaccgcatctccgggctccccgacgacctcctctttgtcatcctctccaagctccccgtcagggatgccgtggcgacgtccgccctctccccccgctggaagagcctctggtccagcgtcccgctccgcctcgacgacgccggcctcctccaccgccgcgacggcacgcgcctcggccgggaaggcgtcgccgccaccgtctccgccgtcctcgccgcccacccaggccccgtgcccgcggcctccgttgggtgctgcctctcctccgacgaccagggctaccagctgggaggctggctccgagccctcgccgccaagggcgttcggcaactgtgcctgatgggcgcgccatggtcgccgcgcgctgctctcccctccgctgtgttctcctgctcatccctgcggcgcctcttccttggttccgtgcaatgcaactgggacctcataccagaccatgcctgcttccctgagctacgagagatccagatatgcaacgccctaatgaagagccaggatctgtcccttgttttggctgtctgcccagcgcttgagacagtggagattcttgcgagccgtaacaaaatccccaccgtgcgaatgagtagccatactatccgcaatactttgctatggaagtcggtggccaaggaagtgaacgttttggacaccccttgcctcagcagggttgttttgtggcaggatcttctgctgccccactcaagatataattctaaggtcacaatcagccgtgccaccaaaatgcgcatttccggttacctggacaccggcatcaacactttggtgatcaacgaaaccacagttaaggtaaacacaaatataagcttcaagacacttatccccagcgtgaaggttttgggtctctctgttcatttcggagtacgaaaggaggctttgatgtccatcagcttccttaggtgttttccagaggttgagacactacatataactagcaaaacggacaaggcttcagaagccgaacaattcagtttctggggaaaggttgatcctgttgagtgtgtgacctctcatctcaagaagttagtgttccatgggatgccatggtgtcctggaaaccttgagtttctcaaattcatcgtggaaggggcatacttgctagagaaggtgttgattgttcttcctaaaggaacttacactagcatgcatagcgttatcaccaaactcaagttagcacctttgacctctgcaagttgggctagtcacatctgtaaaatggaggttgtccaatccagtcaaggaactttgagctaccaaagggcatctgatcattccgtcgatgatcctttagattattccctgtag</cdnaseq> |
| Protein Sequence |
<aaseq>MEAVIAECRPKPLFTTGPFLSAVGGGGGGVDRISGLPDDLLFVI LSKLPVRDAVATSALSPRWKSLWSSVPLRLDDAGLLHRRDGTRLGREGVAATVSAVLA AHPGPVPAASVGCCLSSDDQGYQLGGWLRALAAKGVRQLCLMGAPWSPRAALPSAVFS CSSLRRLFLGSVQCNWDLIPDHACFPELREIQICNALMKSQDLSLVLAVCPALETVEI LASRNKIPTVRMSSHTIRNTLLWKSVAKEVNVLDTPCLSRVVLWQDLLLPHSRYNSKV TISRATKMRISGYLDTGINTLVINETTVKVNTNISFKTLIPSVKVLGLSVHFGVRKEA LMSISFLRCFPEVETLHITSKTDKASEAEQFSFWGKVDPVECVTSHLKKLVFHGMPWC PGNLEFLKFIVEGAYLLEKVLIVLPKGTYTSMHSVITKLKLAPLTSASWASHICKMEV VQSSQGTLSYQRASDHSVDDPLDYSL</aaseq> |
| Gene Sequence |
<dnaseqindica>1..915#1002..1145#1362..1733#atggaggcggtcatcgcggagtgccgccccaagcctctcttcaccaccgggcccttcctctccgccgtcggcggcggcggcggcggcgtcgaccgcatctccgggctccccgacgacctcctctttgtcatcctctccaagctccccgtcagggatgccgtggcgacgtccgccctctccccccgctggaagagcctctggtccagcgtcccgctccgcctcgacgacgccggcctcctccaccgccgcgacggcacgcgcctcggccgggaaggcgtcgccgccaccgtctccgccgtcctcgccgcccacccaggccccgtgcccgcggcctccgttgggtgctgcctctcctccgacgaccagggctaccagctgggaggctggctccgagccctcgccgccaagggcgttcggcaactgtgcctgatgggcgcgccatggtcgccgcgcgctgctctcccctccgctgtgttctcctgctcatccctgcggcgcctcttccttggttccgtgcaatgcaactgggacctcataccagaccatgcctgcttccctgagctacgagagatccagatatgcaacgccctaatgaagagccaggatctgtcccttgttttggctgtctgcccagcgcttgagacagtggagattcttgcgagccgtaacaaaatccccaccgtgcgaatgagtagccatactatccgcaatactttgctatggaagtcggtggccaaggaagtgaacgttttggacaccccttgcctcagcagggttgttttgtggcaggatcttctgctgccccactcaagatataattctaaggtcacaatcagccgtgccaccaaaatgcgcatttccggttacctggacaccggcatcaacactttggtgatcaacgaaaccacagttaaggtatttgtccctttgttggatatatatctctaatcatatcgaatctgtgaaactgtgttaacgcgttttaatcgttgaatttgaaggtaaacacaaatataagcttcaagacacttatccccagcgtgaaggttttgggtctctctgttcatttcggagtacgaaaggaggctttgatgtccatcagcttccttaggtgttttccagaggttgagacactacatataactgtgagatttctttccttctttcattcccgacatatatatacataaatgtgagattgcgagatagctgtctttcttctttcattcctgacatatgcatcattttacacactcactgcagccattctttcattcccgacatatgcatcattttacacactcactgcagccatatgttcctttgatagctggaggtgatcaatttttcttcttgtgcagagcaaaacggacaaggcttcagaagccgaacaattcagtttctggggaaaggttgatcctgttgagtgtgtgacctctcatctcaagaagttagtgttccatgggatgccatggtgtcctggaaaccttgagtttctcaaattcatcgtggaaggggcatacttgctagagaaggtgttgattgttcttcctaaaggaacttacactagcatgcatagcgttatcaccaaactcaagttagcacctttgacctctgcaagttgggctagtcacatctgtaaaatggaggttgtccaatccagtcaaggaactttgagctaccaaagggcatctgatcattccgtcgatgatcctttagattattccctgtag</dnaseqindica> |
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