Os01g0704100
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Annotated Information
The gene name of High-affinity Nitrate Transporter is OsNRT2.3, it located on Chromosome 1.
Function
When the rice in a low nitrate supply environment , OsNRT2.3 plays an important role in nitrate and long distance transport from root to shoot. On the one hand, overexpression of OsNRT2.3 can not only improve the total dry matter's accumulation and translocation in the later stage, but also can significantly increase dry matter's translocation efficiency and contribution rate of transferred dry matter in rice. On the other hand, overexpression of OsNRT2.3 can not only increase the amount of nitrogen accumulation and nitrogen translocation amount, but also can significantly increase rice N translocation rate and nitrogen contribution rate in the later stage. OsNRT2.3 gene silencing could inhibit N03- transport to the shoot from underground, which leads to the accumulation of NO3- in rice roots, and thus indirectly down regulated OsNRT2.1/2.2 expression, eventually lead to reduced nitrate uptake in rice roots from the environment.
Expression
In the field growth conditions, OsNRT2.3 mainly express in the root, it also express in the leaf and other parts in a low expression level. In the whole growing period, the amount of OsNRT2.3 expression in leaves increased with the growth of the plant, it reaches a maximum value in the vegetative growth to reproductive growth transition stage , then decrease to a relatively stable range. Rice protoplast subcellular localization experiments showed that OsNRT2.3 is transmembrane protein of plasma membrane localization, this is consistent with bioinformatics prediction results. OsNRT2.3aWas Localized to the Plasma Membrane To determine subcellular location, OsNRT2.3a was fused in frame with GFP and transiently expressed in rice protoplasts under the control of the cauliflower mosaic virus 35S promoter. Analyses ofOsNRT2.3a -GFP-expressing rice protoplast cells by confocal micros-copy showed that the green fluorescence was confined to the plasma membrane. Thus, OsNRT2.3a is a plasma membrane-localized nitrate transporter. OsNRT2.3aWas Mainly Expressed in Root Stelar Cells
Evolution
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Labs working on this gene
State Key Laboratory of Crop Genetics and Ge rmplas m E nhancement a nd Ke y Laboratory of Plant Nutrition and F erti lization in L ow -Mid dle R each es of th e Yan gtze River, Ministry of Agriculture, Nanjing Agricultural University, N anjing 210095, China ; Disease a nd Stress Biology Department, J ohn Innes Center, N orwich NR4 7UH, U ni te d Kingdom
References
Zhong Tang;Xiaorong Fan;Qing Li;Huimin Feng;Anthony J. Miller;Qirong Shen;Guohua Xu Knockdown of a Rice Stelar Nitrate Transporter Alters Long-Distance Translocation But Not Root Influx Plant Physiology, 2012, 160(4): 2052-2063
Structured Information
| Gene Name |
Os01g0704100 |
|---|---|
| Description |
Similar to Membrane transporter |
| Version |
NM_001050538.1 GI:115439446 GeneID:4324249 |
| Length |
2087 bp |
| Definition |
Oryza sativa Japonica Group Os01g0704100, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 1:30944695..30946781 |
| Sequence Coding Region |
30944917..30945104,30945195..30946467 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008394:30944695..30946781 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008394:30944695..30946781 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggaggctaagccggtggcgatggaggtggagggggtcgaggcggcggggggcaagccgcggttcaggatgccggtggactccgacctcaaggcgacggagttctggctcttctccttcgcgaggccacacatggcctccttccacatggcgtggttctccttcttctgctgcttcgtgtccacgttcgccgtgttcgcgcgtctggccatgggcacggcgtgcgacctggtcgggcccaggctggcctccgcgtctctgatcctcctcaccacaccggcggtgtactgctcctccatcatccagtccccgtcggggtacctcctcgtgcgcttcttcacgggcatctcgctggcgtcgttcgtgtcggcgcagttctggatgagctccatgttctcggcccccaaagtggggctggccaacggcgtggccggcggctggggcaacctcggcggcggcgccgtccagctgctcatgccgctcgtgtacgaggccatccacaagatcggtagcacgccgttcacggcgtggcgcatcgccttcttcatcccgggcctgatgcagacgttctcggccatcgccgtgctggcgttcgggcaggacatgcccggcggcaactacgggaagctccacaagactggcgacatgcacaaggacagcttcggcaacgtgctgcgccacgccctcaccaactaccgcggctggatcctggcgctcacctacggctacagcttcggcgtcgagctcaccatcgacaacgtcgtgcaccagtacttctacgaccgcttcgacgtcaacctccagaccgccgggctcatcgccgccagcttcgggatggccaacatcatctcccgccccggcggcgggctactctccgactggctctccagccggtacggcatgcgcggcaggctgtgggggctgtggactgtgcagaccatcggcggcgtcctctgcgtggtgctcggaatcgtcgacttctccttcgccgcgtccgtcgccgtgatggtgctcttctccttcttcgtccaggccgcgtgcgggctcaccttcggcatcgtgccgttcgtgtcgcggaggtcgctggggctcatctccgggatgaccggcggcgggggcaacgtgggcgccgtgctgacgcagtacatcttcttccacggcacaaagtacaagacggagaccgggatcaagtacatggggctcatgatcatcgcgtgcacgctgcccgtcatgctcatctacttcccgcagtggggcggcatgctcgtaggcccgaggaagggggccacggcggaggagtactacagccgggagtggtcggatcacgagcgcgagaagggtttcaacgcggccagcgtgcggttcgcggagaacagcgtgcgcgagggcgggaggtcgtcggcgaatggcggacagcccaggcacaccgtccccgtcgacgcgtcgccggccggggtgtga</cdnaseq> |
| Protein Sequence |
<aaseq>MEAKPVAMEVEGVEAAGGKPRFRMPVDSDLKATEFWLFSFARPH MASFHMAWFSFFCCFVSTFAVFARLAMGTACDLVGPRLASASLILLTTPAVYCSSIIQ SPSGYLLVRFFTGISLASFVSAQFWMSSMFSAPKVGLANGVAGGWGNLGGGAVQLLMP LVYEAIHKIGSTPFTAWRIAFFIPGLMQTFSAIAVLAFGQDMPGGNYGKLHKTGDMHK DSFGNVLRHALTNYRGWILALTYGYSFGVELTIDNVVHQYFYDRFDVNLQTAGLIAAS FGMANIISRPGGGLLSDWLSSRYGMRGRLWGLWTVQTIGGVLCVVLGIVDFSFAASVA VMVLFSFFVQAACGLTFGIVPFVSRRSLGLISGMTGGGGNVGAVLTQYIFFHGTKYKT ETGIKYMGLMIIACTLPVMLIYFPQWGGMLVGPRKGATAEEYYSREWSDHEREKGFNA ASVRFAENSVREGGRSSANGGQPRHTVPVDASPAGV</aaseq> |
| Gene Sequence |
<dnaseqindica>223..410#501..1773#agcgccggcctcccaccggtcgcgtaagatcacgcccgaaatctttattcattttctctccaccggttgccctctcgccgcacccaaccatcgcgccacgccgcgccgcgctgccggagccgcgctttccgctatgctataagagctgacgcgcagggcacagcggatgtacgtacacacagtcactagctaagctgctagccttgctaccacgtgttggagatggaggctaagccggtggcgatggaggtggagggggtcgaggcggcggggggcaagccgcggttcaggatgccggtggactccgacctcaaggcgacggagttctggctcttctccttcgcgaggccacacatggcctccttccacatggcgtggttctccttcttctgctgcttcgtgtccacgttcgccgcgccgccgctgctgccgctcatccgcgacaccctcgggctcacggccacggacatcggcaacgccgggatcgcgtccgtgtcgggcgccgtgttcgcgcgtctggccatgggcacggcgtgcgacctggtcgggcccaggctggcctccgcgtctctgatcctcctcaccacaccggcggtgtactgctcctccatcatccagtccccgtcggggtacctcctcgtgcgcttcttcacgggcatctcgctggcgtcgttcgtgtcggcgcagttctggatgagctccatgttctcggcccccaaagtggggctggccaacggcgtggccggcggctggggcaacctcggcggcggcgccgtccagctgctcatgccgctcgtgtacgaggccatccacaagatcggtagcacgccgttcacggcgtggcgcatcgccttcttcatcccgggcctgatgcagacgttctcggccatcgccgtgctggcgttcgggcaggacatgcccggcggcaactacgggaagctccacaagactggcgacatgcacaaggacagcttcggcaacgtgctgcgccacgccctcaccaactaccgcggctggatcctggcgctcacctacggctacagcttcggcgtcgagctcaccatcgacaacgtcgtgcaccagtacttctacgaccgcttcgacgtcaacctccagaccgccgggctcatcgccgccagcttcgggatggccaacatcatctcccgccccggcggcgggctactctccgactggctctccagccggtacggcatgcgcggcaggctgtgggggctgtggactgtgcagaccatcggcggcgtcctctgcgtggtgctcggaatcgtcgacttctccttcgccgcgtccgtcgccgtgatggtgctcttctccttcttcgtccaggccgcgtgcgggctcaccttcggcatcgtgccgttcgtgtcgcggaggtcgctggggctcatctccgggatgaccggcggcgggggcaacgtgggcgccgtgctgacgcagtacatcttcttccacggcacaaagtacaagacggagaccgggatcaagtacatggggctcatgatcatcgcgtgcacgctgcccgtcatgctcatctacttcccgcagtggggcggcatgctcgtaggcccgaggaagggggccacggcggaggagtactacagccgggagtggtcggatcacgagcgcgagaagggtttcaacgcggccagcgtgcggttcgcggagaacagcgtgcgcgagggcgggaggtcgtcggcgaatggcggacagcccaggcacaccgtccccgtcgacgcgtcgccggccggggtgtgaagaatgccacggacaataaggtcgcggttgtagtacaactgtacaaattgatggtacgtgtcgtttgaccgcgcgcgcgcacagtgtgggtcgtggcctcgtgggcttagtggagtacagtgaggggtgtacgtgtgtcgtggcgcgcgcggtcacctcggtggccttgggattgggggggcactatacgctagtactccagatatatacgggtttgatttacttctgtggatcggcgcttgttggtggtttgctccctgtggtttttgtgatggtaatcatactcatactcaaacagtcaaaactttttgatg</dnaseqindica> |
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