Os11g0648000

OsNHX3(Os11g0648000), is a Na⁺‡and H⁺‡exchanger in rice (Oryza sativa).

Annotated Information

Function

Figure 1. Genomic organization of the OsNHX3 genes.(from reference ).

• OsNHX3 can suppress the Na⁺, Li⁺, and hygromycin sensitivity of yeast nhx1 mutants and their sensitivity to a high K⁺ concentration. The expression of OsNHX1, OsNHX2, OsNHX3, and OsNHX5 is regulated differently in rice tissues and is increased by salt stress, hyperosmotic stress, and ABA.

• The genomic organization of OsNHX3 is graphically presented in Figure 1. OsNHX3 had 15 exons.

GO assignment(s): GO:0006814, GO:0006885, GO:0015299, GO:0015385, GO:0016021

Expression

Figure 2. Expression of OsNHX1, 2, 3, and Os09g0286400 in rice tissues.(from reference ).

Fukuda et al. examined the expression of OsNHX1, OsNHX2, OsNHX3, and OsNHX5 in various rice tissues by Northern-blot analysis with total RNA extracted from 8-day-old rice seedlings and 12-weekold rice (7 days after heading). Expression of these genes was regulated differently in different rice tissues (Fig. 2). Compared with other tissues, those of OsNHX3 were higher in flag leaf sheaths and blades

• Treatment with salt stress, hyperosmotic stress (mannitol), and ABA increased the transcript levels of OsNHX1, OsNHX2, OsNHX3, and OsNHX5 in rice seedlings.

• Treatment with high concentrations of KCl scarcely changed transcript levels of OsNHX3 (Fig. 5b). In Figure 3, OsNHX3-mediated less K⁺ tolerance than the other family genes in yeast mutants. These results suggest that OsNHX3 might have low transport capacity of K⁺ and not function in the tolerance of rice seedlings to high concentrations of KCl.

• The expression of OsNHX1, OsNHX2, OsNHX3, and OsNHX5 might be regulated through different ABA-dependent pathways.

Evolution

Figure 3. Phylogenetic analysis of Na⁺/H⁺ antiporter proteins.(from reference ).

Figure 4. Phylogenetic tree of intracellular NHE/NHX exchangers.(from reference ).

• The OsNHX proteins shared between 29 and 75% identity. OsNHX1 through 4 in particular shared high similarity, with more than 70% identity among OsNHX1 through 3 and more than 50% identity among OsNHX1 through 4. The sequence of LFFIYLLPPI, which is identified as the binding site of amiloride, an inhibitor of eukaryotic Na⁺/H⁺ antiporters, was identical among OsNHX1 through 3 and highly conserved in OsNHX4 and 5. The membrane-spanning segments M5 and M6 of OsNHX1, which are well conserved in the eukaryotic Na⁺/H⁺ antiporters, also shared high similarity with OsNHX2 through 5, and all of the OsNHX proteins contained the residues important for Na⁺/H⁺ antiport activity.

• OsNHX1 through 4, AtNHX1 through 4, and other most plant NHXtype antiporters are classified into a type I group, and OsNHX5, AtNHX5, AtNHX6, and LeNHX2 from L. esculentum are classified into the type II group (Fig. 3).

• Arabidopsis contains six NHX genes and rice has five, which are distributed in a similar way: AtNHX1–4 and OsNHX1–4 constitute class

I, and AtNHX5–6 and OsNHX5 constitute class II (Fig. 4). Members of the class-I category of Arabidopsis and rice show 54–87% similarity. Similarity among class-II members is 72–79%, but they are only 21–23% similar to class-I isoforms. These data indicate that divergence between class-I and class-II exchangers in plants occurred before the separation of dicotyledons and monocotyledons.

• Pires et al. observe that multiple independent duplication events have occurred throughout the evolutionary history of the NHX family. Based on the reconciled phylogeny, Pires et al. estimate 27 independent gene duplication and 40 gene loss events during the diversification of this gene family.

Labs working on this gene

Please input related labs here.

References

Please input cited references here.

Structured Information

Gene Name	Os11g0648000
Description	Similar to Na+/H+ antiporter
Version	NM_001074903.1 GI:115486454 GeneID:4351027
Length	4072 bp
Definition	Oryza sativa Japonica Group Os11g0648000, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 11
Location	Chromosome 11:27611114..27615185
Sequence Coding Region	27611400..27611741,27611875..27611958,27612174..27612236,27612323..27612461,27612565..27612672 ,27612803..27612903,27613014..27613080,27613181..27613227,27613303..27613544 ,27613637..27613696,27613775..27613872,27614376..27614494,27614613..27614780
Expression	GEO Profiles:Os11g0648000
Genome Context	<gbrowseImage1> name=NC_008404:27611114..27615185 source=RiceChromosome11 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008404:27611114..27615185 source=RiceChromosome11 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggagctcgggttggggatggggatggggctgggcgacccgcctgcggactacggctcgatcgcggcggtggggatgttcgtggcgctcatctgcgtctgcatcgtcgtcggccacctcctcgaggagagccgatggatgaacgagtccatcaccgcgctaatcatcgggttgggtactggaggagtgattttgatggtgtcgagctggaagcactcgcgcatactggtgttcagcgaggatctcttcttcatttacctgctcccgccgatcatattcaatgcggggttccaagttaagaagaaacaatttttccgcaacttcatgactattacgttgtttggtgcgattgggaccctaatctctttcagtgtaatttccctcggttctctaggactaatatcaaggctgaacataggttcccttgatcttggagattatcttgcacttggggcaatattctcggcaacagactctgtatgcaccttgcaggttttgaaccaagatgaaacacccttcttgtacagcctggttttcggggaaggtgttgtcaatgatgcaacatcagttgtgctattcaacgcaatgcagaactttgatcttgcaaatttcagcagtgttaaattcttgcaattcattggcaattttctttatctgtttgccacgagcacctttcttggagttgctgctggacttctcagtgcttatatcataaaaaagctgtacttcggcaggcattccactgatcgtgaagtttctattatgatgctcatggcttacttgtcttacatgcttgctgaattgcttgatttgagtggtattcttacggtgttcttctgtggtattgtaatgtcgcactatacctggcataatgtaacagagagttccagggtcacaaccaagcacgccttcgccacattgtcatttatcgccgagacatttctatttctttatgttggtatggatgcattggatatggagaagtggaagattgtcggtgaaacattcagcccaatgaaatctattgctttgagctccactattttgttcttggtgctggtcgcaagagctgcttttgttttcccactatcttttttggcaaatttgaccaaaaaaactgaagagggaaagatctctattaagcagcaagttattatttggtgggcgggtctgatgagaggtgctgtgtccattgcattggcctacaacaagttcacaaggtcaggccacactcaactccctagtaatgctatcatgatcactagtacaatcactgttgttcttttcagcacgatggtctttgggctcctgactaagccattaatcagactcctaattccagcaagacacctcaaccgggaatcgagtgcgctttcggatccacctagcccgaaatccttccttgatccactgatcctgaacggttccgatgtcgatcctgagattggtgtgggcattcgccgaccgacaagcctccgacttctcctagcaagcccgacacagtcggtccaccattactggcgcaagttcgacaacgccttcatgaggccggtgtttgggggccgaggattcgtccccttcgtccccggctcaccaactgaaaggagtgtgccattactccagggaaatgagaactag</cdnaseq>
Protein Sequence	<aaseq>MELGLGMGMGLGDPPADYGSIAAVGMFVALICVCIVVGHLLEES RWMNESITALIIGLGTGGVILMVSSWKHSRILVFSEDLFFIYLLPPIIFNAGFQVKKK QFFRNFMTITLFGAIGTLISFSVISLGSLGLISRLNIGSLDLGDYLALGAIFSATDSV CTLQVLNQDETPFLYSLVFGEGVVNDATSVVLFNAMQNFDLANFSSVKFLQFIGNFLY LFATSTFLGVAAGLLSAYIIKKLYFGRHSTDREVSIMMLMAYLSYMLAELLDLSGILT VFFCGIVMSHYTWHNVTESSRVTTKHAFATLSFIAETFLFLYVGMDALDMEKWKIVGE TFSPMKSIALSSTILFLVLVARAAFVFPLSFLANLTKKTEEGKISIKQQVIIWWAGLM RGAVSIALAYNKFTRSGHTQLPSNAIMITSTITVVLFSTMVFGLLTKPLIRLLIPARH LNRESSALSDPPSPKSFLDPLILNGSDVDPEIGVGIRRPTSLRLLLASPTQSVHHYWR KFDNAFMRPVFGGRGFVPFVPGSPTERSVPLLQGNEN</aaseq>
Gene Sequence	<dnaseqindica>3445..3786#3228..3311#2950..3012#2725..2863#2514..2621#2283..2383#2106..2172#1959..2005#1642..1883#1490..1549#1314..1411#692..810#406..573#atactatctcgaaacgataaaaaagagcacaaattcttctcctgttagtaattctgttcgaattcctctccttccccaaaaaagagtggagttcatctccgccacgcggggttaccgataaaaatcccccaaatttccgctcccaccccgtcggattctcccccgaatccgagagttcatcgttttccgccgatttattttatctggttttgtaaaccagcagcccaattgctctctgccttcgtctacctcgtggaagattccgggtcaaattctagactttttggtgggttgcgtgcgaggggtggaggttgcttcgaaggcaagcgttggggggggggggggggattggattttgaggagggagggagggagtagggtttggtaattggaggaggtggagtaatggagctcgggttggggatggggatggggctgggcgacccgcctgcggactacggctcgatcgcggcggtggggatgttcgtggcgctcatctgcgtctgcatcgtcgtcggccacctcctcgaggagagccgatggatgaacgagtccatcaccgcgctaatcatcgtgagtcgatgagttcttgttgagttgtgctgtttattatgtgagattttgggagatgatggtggttttcgtttggtgtttgattgtttgtggtgtgtgtgtctggggtttcttgcaggggttgggtactggaggagtgattttgatggtgtcgagctggaagcactcgcgcatactggtgttcagcgaggatctcttcttcatttacctgctcccgccgatcatattcaatgcggggtaagctgtttcatgtgaattaagattgtgctatcatggtgatgattttctttataattagagtcgtctttgcaaaagtagtgagtgataaggaagaaaatatgcaggggcaattagttgtgagttccatgctggaacgtgatataattgggttgttactaaatggagctgctattgcttctctctttgaaattatcatgctaattaaggcaatatcacttatgagatgtatgcgtttttgtagtacacgtaaattgtgcctcttcagttctgtagtaggagtatttggaatgggaaattttacttttccacttctttgtatcaaactgattttgctaattaaggtacacctagctatcaaatgcatacacatagtgcacattattatagcaattaaactctctaatcttccagtaggaaattctgttacatcatttaaagtttaacattagtcagcaaggtgaagcttactgttcctttttttctctttctttcctgagcaggttccaagttaagaagaaacaatttttccgcaacttcatgactattacgttgtttggtgcgattgggaccctaatctctttcagtgtaatttccctcggtaagttgttttcctctttaaattttacctggcatgttcttttgtgctcatctctctcactcgtacattccatcacaggttctctaggactaatatcaaggctgaacataggttcccttgatcttggagattatcttggtcagttcttatatgaacctaatgcgtaagcatttcgattgcatgcctgtggttctgtgttttgacttgttccgcatgtgaacttttttcagcacttggggcaatattctcggcaacagactctgtatgcaccttgcaggttttgaaccaagatgaaacacccttcttgtacagcctggttttcggggaaggtgttgtcaatgatgcaacatcagttgtgctattcaacgcaatgcagaactttgatcttgcaaatttcagcagtgttaaattcttgcaattcattggcaattttctttatctgtttgccacgagcacctttcttggagttgctgtaagttctttcctgctcactccaatctttatctttgtcaatgtttctttttcctaaagcatgatatgactgcaggctggacttctcagtgcttatatcataaaaaagctgtacttcggcaggtttgtacaacacaccacattatgtggttgatatttgtatgatcaatcaccagacattttataataattctctaatccatgaattataccttcaatccaggcattccactgatcgtgaagtttctattatgatgctcatggcttacttgtcttacatgcttgctgaagtaagcaccctgtactattagcatcatatagatcctgcagatttgagcaatgtcagctaaatttaattgtaatggataatctgatattaacctgtttctttttgtggcagttgcttgatttgagtggtattcttacggtgttcttctgtggtattgtaatgtcgcactatacctggcataatgtaacagagagttccagggtcacaaccaagtaagtatctttatggtttgtttcagcaaacttattattccattttcatttttttaacttgatgatttcaaatatgtatttcctgaaattgtgtatcacactgatgcttctctaattgtatactctataggcacgccttcgccacattgtcatttatcgccgagacatttctatttctttatgttggtatggatgcattggatatggagaagtggaagattgtcggtgaaacattcaggtatgctctctgtgggtgtgggaagtaaccatatatgttgttcagttgcccaattaatagataataattgtaatatgtgtaacatatttcttatcctgtgcagcccaatgaaatctattgctttgagctccactattttgttcttggtgctggtcgcaagagctgcttttgttttcccactatcttttttggcaaatttgaccaaaaaaactgaagagggaaagatctctattaagcagcaagtgagtgttttatttagcagcatcttaggcttgctatcttctcttataaggggactttcaggttacttatgatgtgggttattcaggttattatttggtgggcgggtctgatgagaggtgctgtgtccattgcattggcctacaacaaggtaacatttgatctgttcgacctgtgctttagcttgtacaagtttgtgtgatcttgaaatttcatatttgactggctgcttgataaagacacgtgcagcttgcctgtttataaactaacttgtttgaggctggattttttttgtttgctttcgtgtattaactattgttcgtaattcatgctttgatctgaaacttgtcacttcgacacttgcagttcacaaggtcaggccacactcaactccctagtaatgctatcatgatcactagtacaatcactgttgttcttttcagcacgatggtaagttctctctcaaacaaacacaaccctgtgcaatacatccttcggttcaaagggagacagtatgaaagctgatccctgaacaattccacaagttggttctgataaaatcatctctcttatgttccctcaggtctttgggctcctgactaagccattaatcagactcctaattccagcaagacacctcaaccgggaatcgagtgcgctttcggatccacctagcccgaaatccttccttgatccactgatcctgaacggttccgatgtcgatcctgagattggtgtgggcattcgccgaccgacaagcctccgacttctcctagcaagcccgacacagtcggtccaccattactggcgcaagttcgacaacgccttcatgaggccggtgtttgggggccgaggattcgtccccttcgtccccggctcaccaactgaaaggagtgtgccattactccagggaaatgagaactagaaaactttttcaaaggagcgtgccattactccagggaaatgagagctagaaaacataaacaaaggttgtgtaaatctgaggtagaagaacaatcagcaaacacagtttctgtaaatttatctgaggtagtaacagcgttcttggtagctgtggtacatgctgatgtgttatgtacctaatattgtagatagcatggatgtatatatataaagcctctgtagtttagcaagaaatggtgctgaaaattttgccacgatttatacaatatggtatgatttttgcattc</dnaseqindica>
External Link(s)	NCBI Gene:Os11g0648000, RefSeq:Os11g0648000