Os01g0104100
The rice Os01g0104100 was reported as OsCOIN in 2007 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os01g0104100 <=> OsCOIN,COIN
Function
- The rice OsCOIN encodes a putative RING finger protein.
- OsCOIN (Oryza sativa cold-inducible) is a novel cold-inducible gene in rice. It can increase tolerance to chilling, salt and drought, and enhance proline level in rice.
Phenotypic analysis
- Over-expression of OsCOIN in transgenic rice lines significantly enhanced their tolerance to cold, salt and drought, accompanied by an up-regulation of OsP5CS expression and an increase of cellular proline level.
Expression
- OsCOIN is expressed in all rice organs and strongly induced by low temperature, ABA, salt and drought.
Subcellular localization
- The subcellular localization analysis showed that OsCOIN is localized in both nuclear and cytoplasm membrane
The subcellular localization of OsCOIN had been studied in vivo using the transient transfection assay. Figure 2d showed that the OsCOIN–GFP fusion protein was located at the nuclear and the cytoplasm membrane under a confocal microscope. Under the same conditions, comparatively, GFP alone was distributed in the whole cell (Fig. 2b). The results suggested that OsCOIN was a protein located at nuclear and cytoplasm membrane.
Labs working on this gene
- Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Science, Beijing 100093, China
References
- ↑ Liu K, Wang L, Xu Y, Chen N, Ma Q, Li F, Chong K. Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice. Planta. 2007 Sep;226(4):1007-16. PubMed PMID: 17549515.
