IC4R009-Genome-2014-24578372
Contents
Project Title
- Construction of Pseudomolecule Sequences of the aus Rice Cultivar Kasalath for Comparative Genomics of Asian Cultivated Rice
The Background of This Project
Plant Culture & Treatment
- Total genomic DNA of Kasalath was extracted from young leaves of a singleplant by using the cetyltrimethylammonium bromide method
- The researchers constructed DNA libraries with insert sizes of 800–1500 bp according to standard manufacturer’s protocols (http://www.
454.com/; Basel, Switzerland) to generate long-read sequencesbyusing Roche454 pyrosequencing technology (GS-FLX Titanium and GS-FLXþ platforms) as described previously.
- The researchers also constructed libraries with insert sizes of 250–400 bp according to the manufacturer’s instructions (Illumina, San Diego, CA, USA) to produce short single or paired-end reads on the Illumina GAIIx or HiSeq 2000 platforms.
Research Findings
Labs working on this Project
- Agrogenomics Research Center, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan *Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan
- Department of Bioscience, Faculty of Applied Bioscience,Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Corresponding Author
- jzwu@affrc.go.jp
- Tel. þ81 29-838-6148. Fax. þ81 29-838-6028.
