Os03g0848700

Bph14 is a gene conferring resistance to Brown planthopper (BPH) at seedling and maturity stages of the rice plant.

Annotated Information

Function

Bph14 was first mapped from the introgression line B5, derived from O. officinalis, with Qbp1 (renamed as Bph14) on chromosome 3 explaining 26.4% of the phenotypic variance of BPH resistance in this population. Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein. Sequence comparison indicates that Bph14 carries a unique LRR domain that might function in recognition of the BPH insect invasion and activating the defense response. Bph14 is predominantly expressed in vascular bundles, the site of BPH feeding. Expression of Bph14 activates the salicylic acid signalling pathway and induces callose deposition in phloem cells and trypsin inhibitor production after planthopper infestation, thus reducing the feeding, growth rate, and longevity of the BPH insects. Bph14 showed a stable resistance in different genetic backgrounds and thus is valuable in development of resistant rice varieties.

Expression

Bph14 have been identified in B5 and mapped on the long arm of rice chromosome 3【2】(B5 is a rice line derived from the wild rice Oryza officinalis and is highly resistant to the BPH biotypes 1 and 2.).Bph14 is predominantly expressed in vascular bundles,the site of BPH feeding.Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein

Evolution

Bph14 shares 83% sequence identity with its allele (Os03g0848700) in Nipponbare. Phylogenetic analysis revealed that Bph14 is closely related to other rice homologs and is divergent from the majority of known plant disease resistance proteins in other species.

Phylogenetic relationships of Bph14 homologs in rice (Os), wheat (Ta), cassava (Me), potato (Sb), tomato (Le), maize (Zm), barley (Hv), and Arabidopsis (At). (Scale bar, 0.1 amino acid substitutions per site.) (from reference ).

Labs working on this gene

• Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
• National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China
• Shandong Rice Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China
• College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Structured Information

Gene Name	Os03g0848700
Description	Similar to TDRGA-1 (Fragment)
Version	NM_001058430.1 GI:115456588 GeneID:4334783
Length	5725 bp
Definition	Oryza sativa Japonica Group Os03g0848700, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 3
Location	Chromosome 3:36544018..36549742
Sequence Coding Region	36544479..36544552,36545320..36549226
Expression	GEO Profiles:Os03g0848700
Genome Context	<gbrowseImage1> name=NC_008396:36544018..36549742 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008396:36544018..36549742 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggcagagctgatggccaccatggtggtcgggccactgctgtccatggtgaaggacaaggcctccagctacctcctggagcagtacaaggtcatggagggcatggaggagcagcatgagattctcaaacgcaagctgcccgccatcctcgacgtcatcgccgacgccgaggagcaggcggcaaaacacagggaaggggcgaaagcatggcttgaggagctccggaaggtggcctaccaggccaatgatgtcttcgacgagttcaagtacgaggcactccgccgcaaagccaaggccaaggggcactacaagaagctcggcagcattgttgttataaagctcattcctactcacaaccgtattctgtttcgttataggatgggcaacaagctcaggatgatcctgaatgccattgaagtcctaattgcagagatgaatgcctttaggtttaagttccgaccagagccaccaatgtcctccatcaaatggagaaagacggattctaaaatctccaacctttctatggacattgccaacaagtcaagaaagaaagacaaagaggagattgtcaatcgattgcttgctcaagccagcaatggggatctcactgttattcccattgtaggaatgggggggatgggcaagaccaccttagcgcagctcgtttacaatgaccctgaaattcagaagcattttcagttgctgctctggttgtgtgtctctgacaacttcgatgtggattcgctggcaaaaagaatagttgaagcagctcccaaagaaatgaataagaagaatgataatggaggggccaaaaagttaccacaggatgaacttaaagaagttgtgagtgggcagaggtacctcctcattttggatgatgtctggaaccgtgatgccagtaagtgggaagcactcaagtacaaccttaagcatggtggcagcggtagctcagttttgacaacaactcgtgatcaagcagttgctcaactaatggctccagctcaagaagtttatgatctcaagaacctgaatgaaagcttcattgaggaaattatcaagagaagtgctttcaattcagaacaagaaaggcctcctcctgagctactcgaaatggttggtgatattgccaagaaatgttctggttcccctttagctgcaacagcattgggctctacactgcgtacgaagaccaccaagaaagaatgggacgctatattgagcagaagcacaatttgcgatgaggaaaatggaattttaccaatactcaaactcagttacaattgcttgccatcatatatgcggcaatgtttttccttctgtgctatttttcccaaggatcatgagatcgacgtggaaatgctgatccagttatggatggccaatggttttatcccggagaaacaaggagagtgccctgaaatcattggtaaaagaattttcagtgagttggtgtcaaggtcattttttcaggatgtgaaggggatcccatttgagttccatgatataaagtgctctaagattacttgtaagatccatgaccttatgcatgatgttgcacaatcttccatgggaaaagaatgcgctactatagctacagaacttagtaaaagtgatgattttccttattccgctcgccacttgtttttctcaggtgtcattttcctaaagaaagtatatccaggtatccaaacattgatatgttcctcgcaagaagaattgatacgttcctcgcgagaaatatcaaaatacagttcattgcgagctttaaagatggggggagattcattcctgaaaccaaaatatcttcatcacctgaggtatcttgatttgtcatacagtaaaattgaagcacttcctgaagacataagcatcctatatcatctgcaaacattgaacctttccatttgtgactgtctttgtcaactcccaaacggaatgaagtacatgaccgccctccgtcacttgtacactcacggatgttggaggttaaaaagcatgcctccggacctcggacacctcacttgcctacagacgcttacatgttttgtagccggttcttgctctggctgcagtgatttgggagagctgcggcagttggaccttggtggtcgactagagctaagaaaactggaaaatgtgacaaaagctgatgcaaaagcagcaaatctcggaaagaaggaaaaactgaccgaattgagcttaagatggactggtcagaagtacaaggaggcacagagtaataatcataaagaggtgctggaaggtctcacgcctcacgaggggctcaaggttctgagtatacttcactgtgggagcagtacatgtccaacatggatgaataaactgcgggacatggtgaagcttgtgttagatggttgcaaaaatctcgagaagcttcctccactgtggcagctgccagctctagaagttctttgccttgaaggactggatggtttaaattgcttgttcaactgtgacatatacacctcctttacattttgcagactgaaggagcttaccttggccagtatgagaaattttgagacatggtgggacacaaatgaggtaaaaggagaagagctgatatttcctgaggttgaaaagctgataatcaaaagttgtcctaggctaactgccttaccaaaagcgtcaaatgtgatttcagaattgtctggcggagttagcaccgtgtgtcattctgcatttccagcattgaaggaaatggaattatatggtttggatatctttcagaaatgggaggcagtcgatggaactccaagggaggaggtaacatttcctcagctttacaaattagatattagacggtgcccagagttgactactctacctgaagcaccaaagctgagagacttaaacatatatgaagtcaatcagcaaatatccctacaggcagccagcagatatattacttcattgtccagtctccacctgcatttgtcaactgatgacacagaaacagcaccggtggccaagcaacaagattcgagtgaattggtgattgaggatgagaaatggaatcataaatctcccctggaacttatggacttaactggctgcaaccttttattctcttacccaagtgcgttggctctgtggacatgttttgttcagctcctagatctgaatatttgggaagtcgatgcgcttgtcgactggccagaagaggtgttccagggcttggtttccttgaggaagttacatattcttcaatgcaagaatctgacaggactcacacaagctcgtgggcaatctacactagcaccaagtgaactcctgccacgtttggagtccctgcagatacgccgttgttattcttttgtagaggtccccaacctaccgacgtctctcaagctattacaaattacggactgccatgacctgaggtccatcatattcaatcagcagcaggatacgacgatgttggtgagtgcagaaagcttcgcacagccggacaagtcatcgttaatatcagggtccaccagcgagaccaacgatcgcgtccttccacgcctagaatctctagtaatagagtattgcaatcgtttgaaggttctccatcttcctccgtccatcaagaaattggatattgtacgctgtgaaaaacttcagtcactctcaggaaagctggatgccgttcgagcattaaatatcagctattgcgggagcttgaaatcactggaatcttgcttaggagagctcccgtcgctgcaacacctcagacttgttaattgcccaggcctggtatccttgccgaaggggcctcaagcatactcatctcttacatctcttgaaattcgttattgttctggtataaatttgcttccaccgagcctgcagcaacgtctggatgacatcgagaataaagaactagatgcctgctatgaagaatcagaggcaaaaccagaaccaaagtctcctcatcgtcaatctgcaatcagtaggctgctgtgcttggagtag</cdnaseq>
Protein Sequence	<aaseq>MAELMATMVVGPLLSMVKDKASSYLLEQYKVMEGMEEQHEILKR KLPAILDVIADAEEQAAKHREGAKAWLEELRKVAYQANDVFDEFKYEALRRKAKAKGH YKKLGSIVVIKLIPTHNRILFRYRMGNKLRMILNAIEVLIAEMNAFRFKFRPEPPMSS IKWRKTDSKISNLSMDIANKSRKKDKEEIVNRLLAQASNGDLTVIPIVGMGGMGKTTL AQLVYNDPEIQKHFQLLLWLCVSDNFDVDSLAKRIVEAAPKEMNKKNDNGGAKKLPQD ELKEVVSGQRYLLILDDVWNRDASKWEALKYNLKHGGSGSSVLTTTRDQAVAQLMAPA QEVYDLKNLNESFIEEIIKRSAFNSEQERPPPELLEMVGDIAKKCSGSPLAATALGST LRTKTTKKEWDAILSRSTICDEENGILPILKLSYNCLPSYMRQCFSFCAIFPKDHEID VEMLIQLWMANGFIPEKQGECPEIIGKRIFSELVSRSFFQDVKGIPFEFHDIKCSKIT CKIHDLMHDVAQSSMGKECATIATELSKSDDFPYSARHLFFSGVIFLKKVYPGIQTLI CSSQEELIRSSREISKYSSLRALKMGGDSFLKPKYLHHLRYLDLSYSKIEALPEDISI LYHLQTLNLSICDCLCQLPNGMKYMTALRHLYTHGCWRLKSMPPDLGHLTCLQTLTCF VAGSCSGCSDLGELRQLDLGGRLELRKLENVTKADAKAANLGKKEKLTELSLRWTGQK YKEAQSNNHKEVLEGLTPHEGLKVLSILHCGSSTCPTWMNKLRDMVKLVLDGCKNLEK LPPLWQLPALEVLCLEGLDGLNCLFNCDIYTSFTFCRLKELTLASMRNFETWWDTNEV KGEELIFPEVEKLIIKSCPRLTALPKASNVISELSGGVSTVCHSAFPALKEMELYGLD IFQKWEAVDGTPREEVTFPQLYKLDIRRCPELTTLPEAPKLRDLNIYEVNQQISLQAA SRYITSLSSLHLHLSTDDTETAPVAKQQDSSELVIEDEKWNHKSPLELMDLTGCNLLF SYPSALALWTCFVQLLDLNIWEVDALVDWPEEVFQGLVSLRKLHILQCKNLTGLTQAR GQSTLAPSELLPRLESLQIRRCYSFVEVPNLPTSLKLLQITDCHDLRSIIFNQQQDTT MLVSAESFAQPDKSSLISGSTSETNDRVLPRLESLVIEYCNRLKVLHLPPSIKKLDIV RCEKLQSLSGKLDAVRALNISYCGSLKSLESCLGELPSLQHLRLVNCPGLVSLPKGPQ AYSSLTSLEIRYCSGINLLPPSLQQRLDDIENKELDACYEESEAKPEPKSPHRQSAIS RLLCLE</aaseq>
Gene Sequence	<dnaseqindica>5191..5264#517..4423#acgggctacagcggcgagtgacgcgcttgcggctgcaggctgctgagctcgtccgtcaagcgccgccttaatttgagacgggtccgagcagctccaaccttgctctccacgaaccggccaagcccaattcgccgttgagttccggtcgacggaggaacacaagaacgacagggcaccacaccggtgagcatcccagcttcttctcagctatcgtcagtacttcgtggttgcgcttgcgccgaccgaccggcttctctcccgcgcactccagttttctatctctatcgtcgcccagttctcggaagcttactgcactgtactgtacttgcttacttcttcattccaatcaaacactttcattccaggcagcaactcttaatactagctagttgcattcagttaaacacattccttcagtttccatttcccacaaaaagcagctgctgctcacactccttgcttattgtgcttccctgtgttctgttcgtgtgttcttagctctaggagatccttccgatggcagagctgatggccaccatggtggtcgggccactgctgtccatggtgaaggacaaggcctccagctacctcctggagcagtacaaggtcatggagggcatggaggagcagcatgagattctcaaacgcaagctgcccgccatcctcgacgtcatcgccgacgccgaggagcaggcggcaaaacacagggaaggggcgaaagcatggcttgaggagctccggaaggtggcctaccaggccaatgatgtcttcgacgagttcaagtacgaggcactccgccgcaaagccaaggccaaggggcactacaagaagctcggcagcattgttgttataaagctcattcctactcacaaccgtattctgtttcgttataggatgggcaacaagctcaggatgatcctgaatgccattgaagtcctaattgcagagatgaatgcctttaggtttaagttccgaccagagccaccaatgtcctccatcaaatggagaaagacggattctaaaatctccaacctttctatggacattgccaacaagtcaagaaagaaagacaaagaggagattgtcaatcgattgcttgctcaagccagcaatggggatctcactgttattcccattgtaggaatgggggggatgggcaagaccaccttagcgcagctcgtttacaatgaccctgaaattcagaagcattttcagttgctgctctggttgtgtgtctctgacaacttcgatgtggattcgctggcaaaaagaatagttgaagcagctcccaaagaaatgaataagaagaatgataatggaggggccaaaaagttaccacaggatgaacttaaagaagttgtgagtgggcagaggtacctcctcattttggatgatgtctggaaccgtgatgccagtaagtgggaagcactcaagtacaaccttaagcatggtggcagcggtagctcagttttgacaacaactcgtgatcaagcagttgctcaactaatggctccagctcaagaagtttatgatctcaagaacctgaatgaaagcttcattgaggaaattatcaagagaagtgctttcaattcagaacaagaaaggcctcctcctgagctactcgaaatggttggtgatattgccaagaaatgttctggttcccctttagctgcaacagcattgggctctacactgcgtacgaagaccaccaagaaagaatgggacgctatattgagcagaagcacaatttgcgatgaggaaaatggaattttaccaatactcaaactcagttacaattgcttgccatcatatatgcggcaatgtttttccttctgtgctatttttcccaaggatcatgagatcgacgtggaaatgctgatccagttatggatggccaatggttttatcccggagaaacaaggagagtgccctgaaatcattggtaaaagaattttcagtgagttggtgtcaaggtcattttttcaggatgtgaaggggatcccatttgagttccatgatataaagtgctctaagattacttgtaagatccatgaccttatgcatgatgttgcacaatcttccatgggaaaagaatgcgctactatagctacagaacttagtaaaagtgatgattttccttattccgctcgccacttgtttttctcaggtgtcattttcctaaagaaagtatatccaggtatccaaacattgatatgttcctcgcaagaagaattgatacgttcctcgcgagaaatatcaaaatacagttcattgcgagctttaaagatggggggagattcattcctgaaaccaaaatatcttcatcacctgaggtatcttgatttgtcatacagtaaaattgaagcacttcctgaagacataagcatcctatatcatctgcaaacattgaacctttccatttgtgactgtctttgtcaactcccaaacggaatgaagtacatgaccgccctccgtcacttgtacactcacggatgttggaggttaaaaagcatgcctccggacctcggacacctcacttgcctacagacgcttacatgttttgtagccggttcttgctctggctgcagtgatttgggagagctgcggcagttggaccttggtggtcgactagagctaagaaaactggaaaatgtgacaaaagctgatgcaaaagcagcaaatctcggaaagaaggaaaaactgaccgaattgagcttaagatggactggtcagaagtacaaggaggcacagagtaataatcataaagaggtgctggaaggtctcacgcctcacgaggggctcaaggttctgagtatacttcactgtgggagcagtacatgtccaacatggatgaataaactgcgggacatggtgaagcttgtgttagatggttgcaaaaatctcgagaagcttcctccactgtggcagctgccagctctagaagttctttgccttgaaggactggatggtttaaattgcttgttcaactgtgacatatacacctcctttacattttgcagactgaaggagcttaccttggccagtatgagaaattttgagacatggtgggacacaaatgaggtaaaaggagaagagctgatatttcctgaggttgaaaagctgataatcaaaagttgtcctaggctaactgccttaccaaaagcgtcaaatgtgatttcagaattgtctggcggagttagcaccgtgtgtcattctgcatttccagcattgaaggaaatggaattatatggtttggatatctttcagaaatgggaggcagtcgatggaactccaagggaggaggtaacatttcctcagctttacaaattagatattagacggtgcccagagttgactactctacctgaagcaccaaagctgagagacttaaacatatatgaagtcaatcagcaaatatccctacaggcagccagcagatatattacttcattgtccagtctccacctgcatttgtcaactgatgacacagaaacagcaccggtggccaagcaacaagattcgagtgaattggtgattgaggatgagaaatggaatcataaatctcccctggaacttatggacttaactggctgcaaccttttattctcttacccaagtgcgttggctctgtggacatgttttgttcagctcctagatctgaatatttgggaagtcgatgcgcttgtcgactggccagaagaggtgttccagggcttggtttccttgaggaagttacatattcttcaatgcaagaatctgacaggactcacacaagctcgtgggcaatctacactagcaccaagtgaactcctgccacgtttggagtccctgcagatacgccgttgttattcttttgtagaggtccccaacctaccgacgtctctcaagctattacaaattacggactgccatgacctgaggtccatcatattcaatcagcagcaggatacgacgatgttggtgagtgcagaaagcttcgcacagccggacaagtcatcgttaatatcagggtccaccagcgagaccaacgatcgcgtccttccacgcctagaatctctagtaatagagtattgcaatcgtttgaaggttctccatcttcctccgtccatcaagaaattggatattgtacgctgtgaaaaacttcagtcactctcaggaaagctggatgccgttcgagcattaaatatcagctattgcgggagcttgaaatcactggaatcttgcttaggagagctcccgtcgctgcaacacctcagacttgttaattgcccaggcctggtatccttgccgaaggggcctcaagcatactcatctcttacatctcttgaaattcgttattgttctggtataaatttgcttccaccgagcctgcagcaacgtctggatgacatcgagaataaagaactagatgcctgctatgaaggtaatcttcagtttcttaaccgtgtacgatttagtggtaaaagtttcgagcgtggtgtctagaacccctagtcaaccattaatatgattatttgtacatagagtacaatgcgcattcataactcacttcttcagctgtgtcatctaaaccctttaaactttgagttgcatttgggtatctaatcgcatacaaaggaatttagtaatatctccagtagctattccttatatatgatgatctcttcctgtgattatgcttgctagtttggactatgtaattaatttttgccgggtcgactatgtaattaattgacttcatttagtcgccaagtgtggcatcatgcaattattatggcaaagctgtattagtcatgatcgaagccacttggtgaacttattcccttctactttgaaccaatactcattgatcatttccctttaagcgtttgatatggacaacactttaaattcgcagagctaactaacgcagcgcttgtctttacatttctttcttttctatatggtatataagggtagaatggtaatttcattcataccgctgcatgcgaggatgacttgacatactcatgtggcatttttgtgggtccaaggactatattaacccaaataattaatttgaaggacttgtttggacgatttgaaacatcgatgactaaattgagcccgaagagaaacttcaggagtcgcattggatattcacccctatatattcaatacatataaattttcttttcttttgtttgccagaatcagaggcaaaaccagaaccaaagtctcctcatcgtcaatctgcaatcagtaggctgctgtgcttggagtagcagtttcaggaccaaatgagagttgtcatcatggtttgtaacgcgtgcttgatgattgatttcgaatgaaattctagccactatccttatgcttactctctctatggtgtgacttccttgtaggatcgttaccgaatctgcaattgttgggttattaattgtgtgattgtatctcatggtagaatgtattacctacctaccttttctcaagataaactccactaaagtcgatcttctgtttggtgttgttgagttgtggtccaaatttatttgcacttaataaatgccagcttctgccgtagcggagcggaggcccgtcgtcgggaggcttgggctggagcgtcgccgggaggcctgcccgtcatcccttttagagttccaccatatatatacctcttgtaagccgccttacggcgatgtaacctcacctcagatatagtaaagatattttgctggctggc</dnaseqindica>
External Link(s)	NCBI Gene:Os03g0848700, RefSeq:Os03g0848700