Os06g0133000

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Please input one-sentence summary here.

Annotated Information

Function

Please input function information here. The Waxy gene (wx) encodes granule-bound starch synthase(GBSS), which is the main gene that effects amylose synthesis. The Waxy allele is responsible for gel consistency (GC) as well as amylose content (AC) and plays a crucial role in the improvement of eating and cooking quality of rice because it regulates both amylose content and gel consistency.(from reference[1])

Expression

Please input expression information here. Analyses of carbohydrate and oil fractions and gene expression profiling on a global scale in the rice waxy mutant GM077 revealed several candidate genes implicated in the carbon reallocation response to an amylose deficiency, including genes encoding AGPase and SUSIBA2-like. We believe that AGP and SUSIBA2 are two promising targets for classical breeding and/or transgenic plant improvement to control the carbon flux between starch and other components in cereal seeds 图片1.jpg

Evolution

Please input evolution information here.

Waxy mutants of cereals have a high content of amylopectin and have been well characterized. However, the allocation of carbon to other components, such as beta-glucan and oils, and the regulation of the altered carbon distribution to amylopectin in a waxy mutant are poorly understood. In this study, we used a rice mutant, GM077, with a low content of amylose to gain molecular insight into how a deficiency of amylose affects carbon allocation to other end products and to amylopectin. We used carbohydrate analysis, subtractive cDNA libraries, and qPCR to identify candidate genes potentially responsible for the changes in carbon allocation in GM077 seeds

Labs working on this gene

Please input related labs here.

1.State Key Laboratory of Rice Biology, China National Rice Research Institute, 31006 Hangzhou, China

2.Department of Plant Biology & Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, P.O. Box 7080, SE 75007, Uppsala, Sweden

3.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

4.Institute of Food Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014,China.

5.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China.

6.Key Laboratory of Biotechnology Research of Yunnan Province , Yunnan Academy of Agricultural Sciences, Kunming 650223 , China.

7.College of Life Science, Yunnan University , Kunming 650091 , China.

8.Chengdu Institute of Biology , Chinese Academy of Sciences, Chengdu 610041,China.

9.Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China.

10.Key Laboratory of Southwest Crop Genetic Resource and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China.

11.Rice Research Institute , Sichuan Agricultural University , Wenjiang 611130 , China.

12.Key Laboratory of Southwest Crop Genetic Resource and Improvement , Ministry of Education, Sichuan Agricultural University , Yacan 625014 , China.

13.Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Agricultural College, Yangzhou University, Yangzhou 225009, China

References

Please input cited references here.

  1. 2. Yan Su;Yuchun Rao;Shikai Hu;Yaolong Yang;Zhenyu Gao;Guanghen Zhang;Jian Liu;Jiang Hu;Meixian Yan;Guojun Dong;Li Zhu;Longbiao Guo;Qian Qian;Dali Zeng Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.) Theoretical and Applied Genetics, 2011, 123(5): 859-867.

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Structured Information

Gene Name

Os06g0133000

Description

Granule-bound starch synthase I, chloroplast precursor (EC 2.4.1.21)

Version

NM_001063239.1 GI:115466209 GeneID:4340018

Length

4953 bp

Definition

Oryza sativa Japonica Group Os06g0133000, complete gene.

Source

Oryza sativa Japonica Group

 ORGANISM  Oryza sativa Japonica Group
           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
           clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome

Chromosome 6

Location

Chromosome 6:1764623..1769575

Sequence Coding Region

1765922..1766260,1766374..1766454,1766562..1766660,1766757..1766846,1766946..1767009
,1767102..1767202,1767293..1767402,1767524..1767767,1767885..1768061
,1768305..1768496,1768603..1768689,1768799..1768927,1769285..1769401

Expression

GEO Profiles:Os06g0133000

Genome Context

<gbrowseImage1> name=NC_008399:1764623..1769575 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1>

Gene Structure

<gbrowseImage2> name=NC_008399:1764623..1769575 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2>

Coding Sequence

<cdnaseq>atgtcggctctcaccacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgccacgggttccagggcctcaagccccgcagccccgccggcggcgacgcgacgtcgctcagcgtgacgaccagcgcgcgcgcgacgcccaagcagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaacgtcgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccctgccatggctgcgaatggccacagggtcatggtgatctctcctcggtacgaccagtacaaggacgcttgggataccagcgttgtggctgagatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcgaccatccgtcattcctggagaaggtttggggaaagaccggtgagaagatctacggacctgacactggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgaggatgttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagcccaatggcatctacaggaatgcaaaggttgctttctgcatccacaacatctcctaccagggccgtttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatgacacgccggtggagggcaggaagatcaactggatgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatcgccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcgagtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcatggccgccgccatcccggagctcatgcaggaggacgtccagatcgttcttctgggtactggaaagaagaagttcgagaagctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcatctcatcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggatgagatacggaacgccctgtgcttgcgcgtccaccggtgggctcgtggacacggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgactgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggcacgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgccggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttga</cdnaseq>

Protein Sequence

<aaseq>MSALTTSQLATSATGFGIADRSAPSSLLRHGFQGLKPRSPAGGD ATSLSVTTSARATPKQQRSVQRGSRRFPSVVVYATGAGMNVVFVGAEMAPWSKTGGLG DVLGGLPPAMAANGHRVMVISPRYDQYKDAWDTSVVAEIKVADRYERVRFFHCYKRGV DRVFIDHPSFLEKVWGKTGEKIYGPDTGVDYKDNQMRFSLLCQAALEAPRILNLNNNP YFKGTYGEDVVFVCNDWHTGPLASYLKNNYQPNGIYRNAKVAFCIHNISYQGRFAFED YPELNLSERFRSSFDFIDGYDTPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIA RGCELDNIMRLTGITGIVNGMDVSEWDPSKDKYITAKYDATTAIEAKALNKEALQAEA GLPVDRKIPLIAFIGRLEEQKGPDVMAAAIPELMQEDVQIVLLGTGKKKFEKLLKSME EKYPGKVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGG LVDTVIEGKTGFHMGRLSVDCKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQ DLSWKGPAKNWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP</aaseq>

Gene Sequence

<dnaseqindica>1300..1638#1752..1832#1940..2038#2135..2224#2324..2387#2480..2580#2671..2780#2902..3145#3263..3439#3683..3874#3981..4067#4177..4305#4663..4779#accattccttcagttctttgtctatctcaagacacaaataactgcagtctctctctctctctctctctctctctctctctctctgcttcacttctctgcttgtgttgttctgttgttcatcaggaagaacatctgcaagttatacatatatgtttataattctttgtttcccctcttattcagatcgatcacatgcatctttcattgctcgtttttccttacaagtagtctcatacatgctaatttctgtaaggtgttgggctggaaattaattaattaattaattgacttgccaagatccatatatatgtcctgatattaaatcttcgttcgttatgtttggttaggctgatcaatgttattctagagtctagagaaacacacccaggggttttccaactagctccacaagatggtgggctagctgacctagatttgaagtctcactccttataattattttatattagatcattttctaatattcgtgtctttttttattctagagtctagatcttgtgttcaactctcgttaaatcatgtctctcgccactggagaaacagatcaggagggtttattttgggtataggtcaaagctaagattgaaattcacaaatagtaaaatcagaatccaaccaattttagtagccgagttggtcaaaggaaaatgtatatagctagatttattgttttggcaaaaaaaaatctgaatatgcaaaatacttgtatatctttgtattaagaagatgaaaataagtagcagaaaattaaaaaatggattatatttcctgggctaaaagaattgttgatttggcacaattaaattcagtgtcaaggttttgtgcaagaattcagtgtgaaggaatagattctcttcaaaacaatttaatcattcatctgatctgctcaaagctctgtgcatctccgggtgcaacggccaggatatttattgtgcagtaaaaaaatgtcatatcccctagccacccaagaaactgctccttaagtccttataagcacatatggcattgtaatatatatgtttgagttttagcgacaatttttttaaaaacttttggtcctttttatgaacgttttaagtttcactgtctttttttttcgaattttaaatgtagcttcaaattctaatccccaatccaaattgtaataaacttcaattctcctaattaacatcttaattcatttatttgaaaaccagttcaaattcttttaggctcaccaaaccttaaacaattcaattcagtgcagagatcttccacagcaacagctagacaaccaccatgtcggctctcaccacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgccacgggttccagggcctcaagccccgcagccccgccggcggcgacgcgacgtcgctcagcgtgacgaccagcgcgcgcgcgacgcccaagcagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaacgtcgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccctgccatggctgtaagcacacacaaacttcgatcgctcgtcgtcgctgaccgtcgtcgtcttcaactgttcttgatcatcgcattggatggatgtgtaatgttgtgttcttgtgttctttgcaggcgaatggccacagggtcatggtgatctctcctcggtacgaccagtacaaggacgcttgggataccagcgttgtggctgaggtaggagcatatgcgtgatcagatcatcacaagatcgattagctttagatgatttgttacatttcgcaagattttaacccaagtttttgtggtgcaattcattgcagatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcgaccatccgtcattcctggagaaggtggagtcatcattagtttaccttttttgtttttactgaattattaacagtgcatttagcagttggactgagcttagcttccactggtgatttcaggtttggggaaagaccggtgagaagatctacggacctgacactggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggtcagtgattacttctatctgatgatggttggaagcatcacgagtttaccatagtatgtatggattcataactaattcgtgtattgatgctacctgcaggcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgagttacaattgatctcaagatcttataactttcttcgaaggaatccatgatgatcagactaattccttccggtttgttactgacaacaggtgaggatgttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagcccaatggcatctacaggaatgcaaaggtctatgcttgttcttgccataccaactcaaatctgcatgcacactgcattctgttcagaaactgactgtctgaatctttttcactgcaggttgctttctgcatccacaacatctcctaccagggccgtttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatgagtaagattctaagagtaacttactgtcaattcgccatatatcgattcaatccaagatccttttgagctgacaaccctgcactactgtccatcgttcaaatccggttaaatttcaggtatgacacgccggtggagggcaggaagatcaactggatgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatcgccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcgagtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggtaagaacgaatgcattcttcacaagatatgcaatctgaattttctttgaaaaagaaattatcatctgtcacttcttgattgattctgacaaggcaagaatgagtgacaaatttcaggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcatggccgccgccatcccggagctcatgcaggaggacgtccagatcgttcttctggtataatataatacactacaagacacacttgcacgatatgccaaaaattcagaacaaattcagtggcaaaaaaaaaactcgaatattagggaaggacctaataatatcaaataattagaaggggtgaggctttgaacccagatcgtctagtccaccaccttgtggagttagccggaagacctctgagcatttctcaattcagtggcaaatgatgtgtataattttgatccgtgtgtgtttcagggtactggaaagaagaagttcgagaagctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcatctcatcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggatgagatacggaacggtatacaatttccatctatcaattcgattgttcgatttcatctttgtgcaatgcaatgcaattgcaaatgcaaatgcatgatgattttccttgttgatttctccagccctgtgcttgcgcgtccaccggtgggctcgtggacacggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgacgtaagcctatacatttacataacaatcagatatgacacatcctaataccgataagtcggtacactactacacatttacatggttgctggttatatggtttttttggcagtgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggcacgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggtataaattacgaaacaaatttaacccaaacatatactatatactccctccgcttctaaatattcaacgccgttgtcttttttaaatatgtttgaccattcgtcttattaaaaaaattaaataattataaattcttttcctatcatttgattcattgttaaatatacttatatgtatacatatagttttacatatttcataaaattttttgaacaagacgaacggtcaaacatgtgctaaaaagttaacggtgtcgaatattcagaaacggagggagtataaacgtcttgttcagaagttcagagattcacctgtctgatgctgatgatgattaattgtttgcaacatggatttcaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgccggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttgaagagcctgagatctacatatggagtgattaattaatatagcagtatatggatgagagacgaatgaaccagtggtttgtttgttgtagtgaatttgtagctatagccaattatataggctaataagtttgatgttgtactcttctgggtgtgcttaagtatcttatcggaccctg</dnaseqindica>

External Link(s)

NCBI Gene:Os06g0133000, RefSeq:Os06g0133000