Os07g0682800
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Annotated Information
Root hairs, projections from the epidermal cells of the roots, are contributing to water and nutrient uptake and anchorage to the soil.
Function
Root hairs, projections from the epidermal cells of the roots, are contributing to water and nutrient uptake and anchorage to the soil.. SEM observation showed that in rth1 plants, root hair elongation was abolished after the formation of bulge. High-resolution mapping using 2,088 segregants revealed three predicted genes in a 38-kb candidate interval on chromosome 7. Sequences comparison of the three genes between wild-type Oochikara and rth1 detected a nucleotide substitution only in apyrase (OsAPY). This nucleotide substitution (G→A) lies in the junction between the third intron and the forth exon, and results in the splicing anomaly to the rth1 cDNA sequence. Transgenic plants with introduced OsAPY allele restored normal root hairs and plant growth, showing a complementation of rth1 phenotype. We concluded that the root hairless phenotype of rth1 is caused by a mutation of OsAPY. OsAPY appears to be an important gene for root hair elongation and plant growth in rice.
Expression
In Arabidopsis, a lot of root hair mutants are available, and many genes involved in the root hair morphogenesis have been isolated (Grierson and Schiefelbein 2002). On the other hand, rice has a more complicated pattern of root hair formation (Kawata and Ishihara 1959). Only two root hair mutants in rice have been reported (Suzuki et al. 2003, Kim et al. 2007), and, causal gene was isolated in only one mutant (Kim et al. 2007). A mutant gene, root hairless 1 (rth1) reported by Suzuki et al. (2003), remains unidentified. Thus, genetic studies on root hairs in rice lag behind compared to Arabidopsis. In the present study, we have identified a new root hair mutant gene, rth1 in rice by means of positional cloning. We describe morphological and physiological characteristics, and gene expression pattern in rth1 mutant. We revealed that RTH1 encodes an enzyme apyrase that can hydrolyze NTPs and/or diphosphates (Shibata et al. 1999) and that apyrase is a key gene for root hair elongation and plant growth in rice.
Evolution
F1 progeny derived from a cross between rth1 and Kasalath showed normal root hairs and plant growth. F2 progeny segregated 1561 plants with normal phenotype and 527 root-hairless plants with short root phenotype, fitting to a 3 : 1 ratio (χ 2 =0.80, 0.3<p<0.4). Therefore, rth1 is considered a recessive mutation. In a population of 116 F2 plants, the rth1 gene mapped roughly to the distal region of the long arm of chromosome 7. For fine mapping, a total of 2,088 F2 plants were studied. The rth1 gene was flanked by STS markers, RK30 and RK20, with the distances of 0.26cM and 0.05 cM, respectively (Fig. 3A). The physical distance between them is about 38 kb. In the rth1 candidate region, three genes are predicted in public databases. Those includenucleic acid binding protein (AK109592), expressed protein (AK065098) and putative apyrase protein (AK066262). The entire coding region of all these genes was sequenced.Comparison of the DNA sequences between WT and rth1 detected a single nucleotide change only in the putative apyrase(OsAPY; ATPdiphosphohydrolase EC.3.6.1.5) gene, and the sequences of two other genes are identical between WT and rth1. Compared to the OsAPY gene of WT, rth1 carries a nucleotide substitution (G→A) in the junction between the third intron and the forth exon (Fig. 3C, D). This substitution caused the splicing anomaly to the rth1 cDNA sequence that shifted the splicing site 5-bp downstream and produced a premature stop codon, resulting in 185-amino acid peptide instead of normal 467-amino acid protein. We confirmed alternation of splicing in rth1 by cDNA sequencing.
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os07g0682800 |
|---|---|
| Description |
Similar to Apyrase-like protein |
| Version |
NM_001067198.1 GI:115474128 GeneID:4344315 |
| Length |
4110 bp |
| Definition |
Oryza sativa Japonica Group Os07g0682800, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 7:29623302..29627411 |
| Sequence Coding Region |
29623630..29623941,29624037..29624165,29624248..29624304,29624406..29624498,29625015..29625220 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008400:29623302..29627411 source=RiceChromosome07 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008400:29623302..29627411 source=RiceChromosome07 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgcgccgctactccgcgctgcccggcggcggcgcccgccccgacacgctcgccgaccgcctccaccgctaccgcggcgtcctcctcgtcatcctcgccccgctcgccctcgtctccctcgtcctcctcctcatgccgcgctcccccgcctcctcctccgccgccgccggcaggaggtggggccccctcgacgccaacaagtacgccgtcattttcgacgccgggagctccgggagccgcgtccacgtcttccgcttcgacgccaacctcgatctgctccacattggcgaccagatcgagctcttcgtccagaaaaagccggggcttagcgagtacgccaataacccgcaggaggctgccaaatcgctcgtctctctccttgaggatgccaaacgagtggttcccgtggaattgcgcggccagactcctgttagagttggggccaccgccgggctaagagcgttgggagcagaaaaatcagaggagattttgcaagcggtcagggatcttcttcgcgaaaagagttcgttcaagacccaaccggattgggttacagttcttgatggacctcaggaaggcgcatatgaatgggttaccatcaattatctgctggggaaattgggaaagacttacgcagacacagttggagtggttgatcttggaggtggatctgtgcagatggcatatgcaattgcagagaaggatgcagtgaaggctcctaaaccatcagagggtgaagattcatatgtgaagaaactgtttcttaaaggaacaacatattatctttatgttcacagctatttgcattatgggttgctggctgctagagcagagatcttaaaagccggcaatggcaaaggttacagctactgtacattggaggggcatcaagggcaatacaaatatggcaatggcaaatttgaagcgtcggcttcaccatctggtgctagttattcaaaatgcagggatgatgtagtgaaagctcttaaagttgaccaagcatgcactcacatgaaatgttcttttggtggcatttggaatggtggtggtggcgctggacagaagaatttgtttgtggcatcatttttcttcgacagggcagctgaggctggatttgtgaatcccaaggcacctgtggctaaggttaaaccttcagattttgaaaaggctgccaagcgtgcatgtaagttaaacttgaaggacgccgaagctgcctaccctggtgtccaaaaggataacattccatatatctgcatggatctcgtttaccagtacaccttacttgtggatggatttggtgttggttctcaccaagagatgaccttggtaaagaaagtcccttacagcaacgcgtttgtggaagcagcatggccactgggaagtgccattgaggttgcatcttga</cdnaseq> |
| Protein Sequence |
<aaseq>MRRYSALPGGGARPDTLADRLHRYRGVLLVILAPLALVSLVLLL MPRSPASSSAAAGRRWGPLDANKYAVIFDAGSSGSRVHVFRFDANLDLLHIGDQIELF VQKKPGLSEYANNPQEAAKSLVSLLEDAKRVVPVELRGQTPVRVGATAGLRALGAEKS EEILQAVRDLLREKSSFKTQPDWVTVLDGPQEGAYEWVTINYLLGKLGKTYADTVGVV DLGGGSVQMAYAIAEKDAVKAPKPSEGEDSYVKKLFLKGTTYYLYVHSYLHYGLLAAR AEILKAGNGKGYSYCTLEGHQGQYKYGNGKFEASASPSGASYSKCRDDVVKALKVDQA CTHMKCSFGGIWNGGGGAGQKNLFVASFFFDRAAEAGFVNPKAPVAKVKPSDFEKAAK RACKLNLKDAEAAYPGVQKDNIPYICMDLVYQYTLLVDGFGVGSHQEMTLVKKVPYSN AFVEAAWPLGSAIEVAS</aaseq> |
| Gene Sequence |
<dnaseqindica>329..640#736..864#947..1003#1105..1197#1714..1919#2399..2493#3120..3334#3417..3606#3699..3805#agcagcgaacccctctctcgccgcagatcccaacgccgcacacacacgcacacgcacccgcacgcgcacgcatttccagctcgcgacgacgcccatctcgacgcgacgcgatccccgccaagtcaaaccccacccgttatactctccactgcgatctcagaggcagaggcagagcccacgccgcgcagatccacctctctccgccctggattccgcggagcaggaggcggtcaagccgtcgccgccgccgccgggccgcatccgctaccgcacgccctcctccccggacctcctcctcgccccgccctccgacgccgacgccaagaacatgcgccgctactccgcgctgcccggcggcggcgcccgccccgacacgctcgccgaccgcctccaccgctaccgcggcgtcctcctcgtcatcctcgccccgctcgccctcgtctccctcgtcctcctcctcatgccgcgctcccccgcctcctcctccgccgccgccggcaggaggtggggccccctcgacgccaacaagtacgccgtcattttcgacgccgggagctccgggagccgcgtccacgtcttccgcttcgacgccaacctcgatctgctccacattggcgaccagatcgagctcttcgtccaggtgatccatccacccaattggatctggatctgctagggatcctttcatctctcaatccatttttacttaatttccccccacttccccttttgcagaaaaagccggggcttagcgagtacgccaataacccgcaggaggctgccaaatcgctcgtctctctccttgaggatgccaaacgagtggttcccgtggaattgcgcggccagactcctgttagagttggggtgagttcttgtacgataccagtagagcttcctattgctgcttggtcatcttctaataataagctttataattcacacacaggccaccgccgggctaagagcgttgggagcagaaaaatcagaggagattttgcaagcggtaaattttgcttttattcacctttgattaattgttgtttgatcgatcactgaatttatattggttgtccctaatattgaatacactcacaatttttgcaggtcagggatcttcttcgcgaaaagagttcgttcaagacccaaccggattgggttacagttcttgatggacctcaggaaggcgcatatgaatgggtacaaaactggcctgatttattcatatggttcagaacattactttgtgctcctgattattagaaatgcctcacatttgtcagattgacaatataagttctttttgtttagaaaaaactattttgttcagcaacaaatctgccaattctcttagagctgacatctgccagcttcaaagatataagttttaaaggacatgataaaaaaagagaaattttcgctttcatgagtgaattaggcaaaataactattttcatttttgaaaaacaaaatttgggcaccagaaacaaatgacgaaacaaaatccaaatgacgaaagctaaacattgaaagtaagggcaccagaaacaacattttccctgaaaatagatgcagatcatctgaatccatgtggtgcactgttctgacagttaattcaccttttgagtgtttaggatctgttcacaactagtgattctatgtctagcttttagtccattaactgataacaagtgttgctcttctctatccttataggttaccatcaattatctgctggggaaattgggaaagacttacgcagacacagttggagtggttgatcttggaggtggatctgtgcagatggcatatgcaattgcagagaaggatgcagtgaaggctcctaaaccatcagagggtgaagattcatatgtgaagaaactgtttcttaaaggaacaacatattatctttatgttcacaggttagtatgaaataccgttgagcttttggctgctgctacattgtcaagaaaaaagttattcagttgtgatacggtaacaatcttcaaaatccatttaagcatctgatggcatattgtcatgcctatttctgatgtctgcctatgccaattcataggttatattctttgcaaagcagacagctgttgtaatcctctaagatactatctcgtattgataaaaatgcaagaatagcgtattaatgcaaatttgagaaacttaagtccagaccaaacgtaagggtacttttatgtgtaaagattctacctaaatatcacgtggtattctatgtaatgttctacagcaaacacttaatctgacatagccacaaacttaatactccttacttttttattatctgctgtataagatgacatcacatgacctgtttttagtctggagtctttgaaccacatatataactgtacacatttcttcacagctatttgcattatgggttgctggctgctagagcagagatcttaaaagccggcaatggcaaaggttacagctactgtacattggaggggcatcaaggtactagtaacatacttctaccatattcttctgctatcatttaactccctgccaagtagcttatgtttgttcctaaaatttcaattgttgttacccttatgcatattttggtcaagggaggaatgtaatgcgtaggctgcattcggaggtggaggttgggaacttcacctcgccacacgcaaaacaaagcgacccattagcgcatgattaattaagcatgagctaaaataaacttgaaaaaaatggattgatataattttgtaaaacaacttacatatagaattttttttgcaaaaaaaaaaaacaccgtttagcagtttggcaaacgtgtgcgtggaaaacgagaaatagaagttgggaagaggtggtaaggaacacacccttatagtggatcgtaacctggtttaaatgaggttaccctcaaattctctatgaaacaaataccttactggtttccttgatattctttccaaccatcagtctgcagttagaattatttatagtgatgtgtataaatctaaatcctcatcaacacaaatgaccaatctgtgacaatagttggagatgcaattttatcttttctatgttggtaagatagttttgttctaattgcattgtgattgtagggcaatacaaatatggcaatggcaaatttgaagcgtcggcttcaccatctggtgctagttattcaaaatgcagggatgatgtagtgaaagctcttaaagttgaccaagcatgcactcacatgaaatgttcttttggtggcatttggaatggtggtggtggcgctggacagaagaatttgtttgtggcatcatttttcttcgacagggcagctgaggtaaggttgttgaactactgaagatgtttgcttttgttgccatgatgatacaccagtaaacaattttctggtctctgctcaggctggatttgtgaatcccaaggcacctgtggctaaggttaaaccttcagattttgaaaaggctgccaagcgtgcatgtaagttaaacttgaaggacgccgaagctgcctaccctggtgtccaaaaggataacattccatatatctgcatggatctcgtttaccagtacaccttacttgtggatggatttggtatgtctcgttaaaactaaatataatcaagaccgcattacagattttgcacaaatttatcactaatttgttttgctcttgcactctcgcaggtgttggttctcaccaagagatgaccttggtaaagaaagtcccttacagcaacgcgtttgtggaagcagcatggccactgggaagtgccattgaggttgcatcttgatagacagacagcagaaagttcagcaagggagctacgatcagagggcgtcatcgccgtaaagttttggatctggagggtgttcgcttgctcggcgctgcgcacgccccaagtgaccattttgttactgaaatcgattgatttaacctgcttctatacagtagcctgataatgagtgatacacaaggacccgccagcttgcatagttgcatacttgcatgcaaggggtatatacattttttttttgtaccattgaaagtaagacgagcctgtcattcttttcttgatctatactactccagttctgt</dnaseqindica> |
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