Os07g0596300
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Contents
Annotated Information
Function
Formin Homology 5(FH5), a type II formin-like protein encoded by Rice Morphology Determinant (RMD), functions as an actin-nucleating protein that regulates rice plant morphology by modulating the microtubule- and microfilament-based cytoskeletal systems. Biochemical assays demonstrated that recombinant FH5 protein can nucleate actin polymerization from monomeric G-actin or actin/profilin complexes, cap the barbed end of actin filaments, and bundle actin filaments in vitro. Moreover, FH5 can directly bind to and bundle microtubules through its FH2 domain in vitro. The rice formin protein FH5 plays a critical role in determining plant morphology by regulating actin dynamics and proper spatial organization of microtubules and microfilaments.
Expression
FH5/RMD encodes a formin-like protein consisting of an N-terminal phosphatase tensin (PTEN)-like domain, an FH1 domain, and an FH2 domain. FH5/RMD is ubiquitously expressed in rice tissues, and its protein localization to the chloroplast surface is mediated by the PTEN domain.
Mutation
The rmd mutants display a bending growth pattern in seedlings, are stunted as adult plants, and have aberrant inflorescence (panicle) and seed shape. Cytological analysis showed that rmd mutants have severe cell elongation defects and abnormal microtubule and microfilament arrays.
Compared with wild-type plants, rmd mutants exhibited changed architecture in both vegetative and reproductive phases. rmd Mutants Display Severe Defects in Cell Elongation and Impaired in Microfilament Organization,Display Defective Microtubule Organization
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os07g0596300 |
|---|---|
| Description |
Actin-binding FH2 domain containing protein |
| Version |
NM_001066706.1 GI:115473144 GeneID:4343793 |
| Length |
6892 bp |
| Definition |
Oryza sativa Japonica Group Os07g0596300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 7:24926636..24933527 |
| Sequence Coding Region |
24927271..24927363,24927449..24927588,24927919..24927985,24928076..24928144,24928599..24928715 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008400:24926636..24933527 source=RiceChromosome07 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008400:24926636..24933527 source=RiceChromosome07 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgtcttttggagcacaaacacgaacttttgtccctcctcctccaccccctcctccacctcctaggtcaggtgttggtgggaatactccacctgcacctcctcccccgcctctaaggtcaactgttcctgctatttctccacctccgccaccacctcctccaccactgaaaccaagttctggtgctccatgcccacccccacctccaccgccaccgccacctccccctccctcagccccatcctctcgagcattttcaagtgctcctccaccgccaccaccaccgcccttgttacgatccgttccacctcctcctccaccaccgccaatctcacactctaatgctccaccaccacctccacttccagctgcacgctttaatgctccgccaccgcccccaccacctccaacaacacattttaatgcccctcctccaccgccaccaccgcctatcacacgttcaggagcaccaccttccccacctccaccccctagccctccacctccaccaccacctccaggtgcacgtccaggtcctccacctcctccaccacctccaggtgctcgcccaggtcctccaccacctccaccccctccaggtggtcgtccaagtgctccacctcttcctcctccaggtggccgtgcaagtgctccacctcctccaccccctccaagcacacgtcttggagctccacctcccccgccaccacctggagctggtgggcgagcacctccaccaccgcctgcccctgggggaaggctcggtggtcctcccccacctccacctcctggtggacgtgcaccacctcctcctagaggaccaggagcaccaccacctccaggaggcaatcctagtagtttaataggtagagggcgtggggtggtacgtgcttcaggatcaggttttggggctgcagctgcacgtaaatcaactttaaagccattacattggatcaaggtgactagagctttgcaaggaagtctatgggaagaattacagcgaaatgatgactcacagagtgtttcggagtttgatttgtcagagctggaaagcctttttccagcagctgttccaaaaccgaatgacagctctaaatctgattcacgtagaaaatcgcttggttcaaagccagaaaaagttcacctaattgagttgaggagggcaaacaacacagagatcatgctgacaaaagtgaagatgccactgccagatttggtgagtgcggctttggcactggaccaatctaccttggatgttgatcaagtggagaatctcataaagttctgtcctacaaaagaagaaatggaacttctcaagaattacactggagataaggaaaatctgggtaaatgcgaacagttcttcctagaattgatgaaagtgccacgaatggagtcaaaactgcgggtgttctccttcaagatccagtttggttcgcaagtcgcagatcttcgaaaaagtttgaacaccatagactcttcatgtgatgagattcgaagttctctcaagttaaaagaaatcatgaagaaaattctcctccttgggaatacattgaaccagggtactgctagaggtgctgcagttggcttccggttggacagtcttctaaaactaacagatacccgagcaacaaataataaaatgacacttatgcactacctctgcaaggtacttgctgcaaaatcatcacagcttctggacttctacatggatcttgttagtttagaagctacatcaaagatacaactaaaaatgctggcagaagaaatgcaagcggttagtaaagggctggaaaaggttcagctcgagtataatgcttcagaaagtgatggtccagtatcagagatttttcgtgagaaactgaaagaattcactgacaatgctggagctgatgtgcaatctttgtcttcattattttctgaagtgggaaaaaaggctgatgcactgattaaatatttcggcgaagatcctgtacgctgcccttttgagcaagttatctccaccctcttaacatttgtaacaatgtttcggaaagcacatgaagagaaccgcaagcaagctgagctcgataagaaaagggctgaaaaagaggcagaagcagaaaagtctaaagcacagttggctagtaaaaatgactctaaaccatcaaatccaagccggcaagtgaaacaaacgccagatactaaaacaagagctgcaagcagacgaggaaaagatgtaggttga</cdnaseq> |
| Protein Sequence |
<aaseq>MSFGAQTRTFVPPPPPPPPPPRSGVGGNTPPAPPPPPLRSTVPA ISPPPPPPPPPLKPSSGAPCPPPPPPPPPPPPPSAPSSRAFSSAPPPPPPPPLLRSVP PPPPPPPISHSNAPPPPPLPAARFNAPPPPPPPPTTHFNAPPPPPPPPITRSGAPPSP PPPPSPPPPPPPPGARPGPPPPPPPPGARPGPPPPPPPPGGRPSAPPLPPPGGRASAP PPPPPPSTRLGAPPPPPPPGAGGRAPPPPPAPGGRLGGPPPPPPPGGRAPPPPRGPGA PPPPGGNPSSLIGRGRGVVRASGSGFGAAAARKSTLKPLHWIKVTRALQGSLWEELQR NDDSQSVSEFDLSELESLFPAAVPKPNDSSKSDSRRKSLGSKPEKVHLIELRRANNTE IMLTKVKMPLPDLVSAALALDQSTLDVDQVENLIKFCPTKEEMELLKNYTGDKENLGK CEQFFLELMKVPRMESKLRVFSFKIQFGSQVADLRKSLNTIDSSCDEIRSSLKLKEIM KKILLLGNTLNQGTARGAAVGFRLDSLLKLTDTRATNNKMTLMHYLCKVLAAKSSQLL DFYMDLVSLEATSKIQLKMLAEEMQAVSKGLEKVQLEYNASESDGPVSEIFREKLKEF TDNAGADVQSLSSLFSEVGKKADALIKYFGEDPVRCPFEQVISTLLTFVTMFRKAHEE NRKQAELDKKRAEKEAEAEKSKAQLASKNDSKPSNPSRQVKQTPDTKTRAASRRGKDV G</aaseq> |
| Gene Sequence |
<dnaseqindica>6165..6257#5940..6079#5543..5609#5384..5452#4813..4929#4642..4713#4153..4247#3336..3417#3101..3232#1986..2027#1815..1913#1664..1735#1324..1450#42..1060#gcttgtcaagcattcctcctcctccccctccacctccacttatgtcttttggagcacaaacacgaacttttgtccctcctcctccaccccctcctccacctcctaggtcaggtgttggtgggaatactccacctgcacctcctcccccgcctctaaggtcaactgttcctgctatttctccacctccgccaccacctcctccaccactgaaaccaagttctggtgctccatgcccacccccacctccaccgccaccgccacctccccctccctcagccccatcctctcgagcattttcaagtgctcctccaccgccaccaccaccgcccttgttacgatccgttccacctcctcctccaccaccgccaatctcacactctaatgctccaccaccacctccacttccagctgcacgctttaatgctccgccaccgcccccaccacctccaacaacacattttaatgcccctcctccaccgccaccaccgcctatcacacgttcaggagcaccaccttccccacctccaccccctagccctccacctccaccaccacctccaggtgcacgtccaggtcctccacctcctccaccacctccaggtgctcgcccaggtcctccaccacctccaccccctccaggtggtcgtccaagtgctccacctcttcctcctccaggtggccgtgcaagtgctccacctcctccaccccctccaagcacacgtcttggagctccacctcccccgccaccacctggagctggtgggcgagcacctccaccaccgcctgcccctgggggaaggctcggtggtcctcccccacctccacctcctggtggacgtgcaccacctcctcctagaggaccaggagcaccaccacctccaggaggcaatcctagtagtttaataggtagagggcgtggggtggtacgtgcttcaggatcaggttttggggctgcagctgcacgtaaatcaactttaaagccattacattggatcaaggtgactagagctttgcaaggaagtctatgggaagaattacagcgaaatgatgactcacagaggtatatccttttgattaaaattcttcctccatttgaccatgcttattgatatatgcacataaatttcaatgatgtgattggctgattgctgtaaggcttcatccaggatttgtaaataatttggtgtcatgattgtagcattgaattttctacaattcagttgctaataatctcgtacaatctactccacaaagactggctcaatatttgtgatgcttttttactaggttttgccttaatgtttgtcaatttcttttctgcagtgtttcggagtttgatttgtcagagctggaaagcctttttccagcagctgttccaaaaccgaatgacagctctaaatctgattcacgtagaaaatcgcttggttcaaagccagaaaaagttcacctagtaagtacatgctaattttaatttcctttctaatttgtatcagtgatcaaggtcagaaagacactagatttgttggacatttaagttatgacaaatgtgttacatgatctcattatcatatcacaaaactatgcaaacagtctcaagaacctgtatttattagactttgtaaatgaccatgttacaatagtcttaacacttttatgtgattagattgagttgaggagggcaaacaacacagagatcatgctgacaaaagtgaagatgccactgccagatttggtggtaattatcttataaaacaagcaaagataatggtaacatgattgattttttatatcttaatggcttaaattttctctagagtgcggctttggcactggaccaatctaccttggatgttgatcaagtggagaatctcataaagttctgtcctacaaaagaagaaatggaacttctcaaggtaatacaatgtttggccttgttgtgatctcatctgaaaactatgttggcttactgtctcgtttttttccagaattacactggagataaggaaaatctgggtaaatgcgaacaggtaatggttatttgccatctagtcatctagtggaattagaatatttgcccattgttactggaattagaatatttgcccattgtgctttattatatattagtcctgtcagcacacataaaaacacattttttgggtcttaattggagattttggcaattttagaatgaaacacaatggtcagagtttaaatgtacataaaacagtcttctggttcctctgcttgataactaatatccctgtttttaacatcaactcaaaacagggtggatactgtgtagaagaggccgtatgatatggctcgataataaactggcaatttggcataggagaaaaatggcactatagctagagaaaagctgaacacagcagatatagatggcatagtgagcagaggctaaatttttgttaagagtgtaggaaatagggtggggggaaactttgtttagctcttacttctaaaatgaatgggcatggtttaaggataactggcacgagagtataaaggtgcacaatagaagaatggaacatctacagagatagatggaaggagcagaggctaaattcatgaacgaatctcctacctcttaaatagataagcctaagaaatagtgctttggataagacgacaactaaataacttctactctctccgtttcatattataagtcgctttgacttttttcctagtcaaacttctttaggtttgatcaagtttatagaaaaatttagcaacatctctaacaccaaattagtttcattaaatgtaacattgaatatattttgataatattttttttgtgttgaagatgttgctatatttttctataaacttagtcaaactaaagtcaaatcgacttataatatgaaacggagggagtaccaatcaaccaattagttatttgtttattttcgttgcttggccttgcaagtatttgatggcctggtcagtccacattcactaaagacattaccttttacattttgagtttttaaagcaaaatggtctcaggtgttatacgtttatgccagcttggtagatccgtatgcattaaacaattattttcctgctgcagttcttcctagaattgatgaaagtgccacgaatggagtcaaaactgcgggtgttctccttcaagatccagtttggttcgcaagtcgcagatcttcgaaaaagtttgaacaccatagactcttcatgtgatgaggttcatttataatcgggattagttttttagcaatcaatattgcttcttatgtaatttatactaagtcgaattcctgttttctaaaaaatatctgcatcatcagattcgaagttctctcaagttaaaagaaatcatgaagaaaattctcctccttgggaatacattgaaccagggtactgctagaggtacaacaactgcattttctctgttacactcaatttagttggtcacattatctttagttattcgttggcatatgaaatgcagctgcatttatgtctaccagctctgcaattttactggcttcttgctaggcagaaatgaagagattgacattgaagttttcctgcaacatgtttaatctctaaaataggaatctttcccacaagttcttttttctttttcttttgttgtttgtttgtggtgtatttttaaaatcattgtgaggaagtgggggcatgtgtgttgagtcttgtaaatattctctccttaatgcaatgatatgcagttctcctgcatatttgagaaaaaaaaataggcacggtgtttttgttggaacaaaaagattgtgcatcctaggaagaatgaataatcaatttaggctgataattcctccctttcttttggagtataggcaacatcctagtttctgaaaaacccatagtaatgcgaccgatgtttctaatttaaatcaattatcaccaccacaaatacaggagtatgtagggctactgatggcttttctcgttcttctagagttcataatgataggttacacatttggtaacattcaaattgtttgttagttcagttgtttctttacactggggcatccttttgattttctttagttaattggttatatattagactattagtactgttcttggatagtttcacatttgttggtttgcttacaggtgctgcagttggcttccggttggacagtcttctaaaactaacagatacccgagcaacaaataataaaatgacacttatgcactacctctgcaaggtacgccttaaacaattcctgttccgctaggttgtcgaaagtgaacatggtagcttttcaccaatagtttacctgcttaactgtaaatcaagtttggactgcatcaaataattttattcaaatcaggaccctgaagtcaatttatttgatggtggaaactgtgagttcatgatttgcatgtcatgttgtaagcaaatatatatacaatgatgcacttccacttaatttattctgtaacaaggttatattactatattctacgatgacttatagtttgagtatctggccataacttctttatagtcctggttaagttcacatttgcttgagttgaaatgattttacatcagtatctatcttgcgtacaccctaacaaaagaaaatgtaatatcaggtacttgctgcaaaatcatcacagcttctggacttctacatggatcttgttagtttagaagctacatcaaaggtttgagttttcttttactattctatcaatcttgtagcatatataatttatttactttcgatatgtatatgctgttctaatcggtgttttcctgaacagatacaactaaaaatgctggcagaagaaatgcaagcggttagtaaagggctggaaaaggttcagctcgagtataatgcttcagaaagtgatggtccagtatcagagatttttcgtgaggtcaaggatctaccccattttgacttatttctgacgtatttaaatattaagttggttccttttagtggattacactacctgctatgtggactaaaattagtaatttcttccccagaaattctacaaaaacgttatccaggcttgttaacagttttttcatgtgcttgttttatgattgtcatctggatgtgcttctcagacagcattacatcaattttactgggacatgcccaaatgagaatctaatatacaatagtaaaagagcctctattggtgatacacctgttactcaagacagatgtacacgtctgatatatatacatgggttactgataaacccgttgtttcaatcaatctgacatgtgaacctgatttaaaagttactttctatttgtgtaaagctgaagagtaaaaatcccgttagttctaaatcggtattgtttgcactgttcagaaactgaaagaattcactgacaatgctggagctgatgtgcaatctttgtcttcattattttctgaagtggtatgtttttgttttcttatatgaagaggaatgcatgctattaacccacattccttctggccttgattatttgttgcattattttgtcagggaaaaaaggctgatgcactgattaaatatttcggcgaagatcctgtacgctgcccttttgagcaaggtaagaatttttatgatcaatgtgacttatttctaaggttgtgcattcagcattttctttttccgagtacctgtcaaggatcaagatactgtcagtcgtgtgagcaccccccactataagcacaaacacattatcatgaaaatcattcttatttaaaatatttcaacccataaatatgtaataccttgcaaagaaacaatttaggataaatcattttctgtagtttgcagctgattgagcatatcttatttctgcattttttatcatcaacatatgtgtttgcttgttttggtatgttgcttaactcttatgttgatcatgatgttgcagttatctccaccctcttaacatttgtaacaatgtttcggaaagcacatgaagagaaccgcaagcaagctgagctcgataagaaaagggctgaaaaagaggcagaagcagaaaagtctaaagcacagttggctagtaaaaatgtattgatccttgacctgtatctttggcaacttcagccaacctcttatatccatcgtatttataaaattcaaaatctgctcacaggactctaaaccatcaaatccaagccggcaagtgaaacaaacgccagatactaaaacaagagctgcaagcagacgaggaaaagatgtaggttgatctccacgaccaactctgttgttgcgcttatgatctgcatgctgggcgaatgcatgacctgctcgatgcacggggagggcaagaaaagttgtgtaaatgatgcagccatcaatggaaacagcatggctgcaccatagcaactgtacatgacgccctttacggtaatgccacatgtgtctgttgcttaacaatacattgataaatgaatcgtataattttctgacaagtttttttttttcttattgcagttcgcactagcgatggccggtttttttctcttcggccagcagggccgcccagtggcaacttctgactgctgtaaagaactaacactaacagcaaagctaacatcattcgttttttcctttgttttttatgttacttgtccattctttctaaccccagtggccaagtgcagccaatgcttcaggagtagagcctcgtagtatcgtagtaggtaggtagaaattgtacaaataggaacagggaaagggtagggaaaccatgtggggcggagaaattgtagctgggatctatacgagatgtaaacatgcgtgtgcttcacgaatatttttttttgtagtgaatgtaccaaggttttcataattagtgaatgcagcagcttttccatgatc</dnaseqindica> 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