Os11g0587000
Contents
Annotated Information
Function
It reports here the molecular genetic characterization of dwarf27(d27), a classic rice mutant exhibiting increased tillers and reduced plant height.dwarf27 encodes a novel iron-containing protein that localizes in chloroplasts and is expressed mainly in vascular cells of shoots and roots. The phenotype of d27 is correlated with enhanced polar auxin transport. D27 is involved in the MAX/RMS/D pathway, in which D27 acts as a new member participating in the biosynthesis of strigolactones.Tillering in rice is one of the most important agronomic traits that determine grain yields and a model system for elucidating molecular mechanisms that regulate axillary buds (Wang and Li,2005). In this study, we characterize a rice dwarf 27 (d27) mutant that is defective in the outgrowth of axillary buds. Map-based cloning and in-depth analysis of D27 revealed that it encodes a novel chloroplast-located iron-containing protein. Our results demonstrate that D27 regulates tiller bud outgrowth through the MAX/RMS/D pathway and participates in the biosynthesis of strigolactones.
Expression
In rice (Oryza sativa), the plastid-localized protein DWARF27 (OsD27) is necessary for SL biosynthesis, but the equivalent gene in Arabidopsis has not been identified.And DWARF27 (D27) have been identified with reduced strigolactone levels or strigolactone response.
To isolate the D27 gene, we took a map-based cloning approach.D27 was primarily delimited in an interval of ; 3.0 centimorgans between the two molecular markers C189 and RM206 on the long arm of chromosome 11 (Figure 2A). To fine-map the D27 locus, we generated a large F2 mapping population derived from a cross between d27-ZF802 and its isogenetic lines ZF802. Of 21,000 F2 plants, 5200 mutant plants were used for fine-mapping, and the D27 locus was located between the two cleaved amplified polymorphic sequence (CAPS) markers P1 and P7 (Figure 2B). Screening with newly developed molecular markers P1 to P7 (see Supplemental Table 1 online), D27 was further placed within an 18-kb DNA region between the P3 and P6 markers and cosegregated with the P5 marker (Figure 2C). Within this region, there are two open reading frames (ORFs).Sequencing of these two ORFs of d27-ZF802 revealed a 4-bp deletion at the fourth exon of a putative gene, ORF LOC_Os11g37650, and this deletion results in a frame shift and generates a premature translation termination product (see Supplemental Figure 2 online).The identity of D27 was further confirmed by a genetic com-plementation test. The plasmid pD27C, containing a 9.25-kbgenomic DNA fragment consisting of a 2236-bp upstream se-quence, the entire D27 gene including seven exons and six introns, and a 2044-bp downstream region (Figure 2D), was introduced into a d27-Nipponbare mutant. All four transgenic lines of pD27C complement the d27 phenotype (Figure 2E).Therefore, ORF LOC_Os11g37650 is the rice D27 gene, and its 4-bp deletion is responsible for the altered phenotype of d27.
Evolution
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Labs working on this gene
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References
1.Wang Y, Li J. Branching in rice[J]. Current opinion in plant biology, 2011, 14(1): 94-99. http://www.ncbi.nlm.nih.gov/pubmed/21144796 Waters M T, Brewer P B, Bussell J D, et al. The Arabidopsis ortholog of rice DWARF27 acts upstream of MAX1 in the control of plant development by strigolactones[J]. 2.Plant physiology, 2012, 159(3): 1073-1085. http://www.ncbi.nlm.nih.gov/pubmed/22623516
Structured Information
| Gene Name |
DWARF27 |
|---|---|
| Description |
Putative uncharacterized protein |
| Version |
FJ641055.1 GI:254946545 Gene ID: 3974662 |
| Length |
5390 bp |
| Definition |
Oryza sativa Japonica Group DWARF27, complete gene. |
| Source |
Oryza sativa Japonica Group (Japanese rice) cultivar = Nipponbare ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome = Chromosome 1 |
| Chromosome |
{{{Chromosome}}} |
| Location |
Chromosome 11:222..5190 |
| Sequence Coding Region |
222..525,2082..2221,2438..2530,2891..2952,3514..3620,4977..5066,5150..5190 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=JaponicaChromosome11:222..5190 source=RiceJaponica11 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=JaponicaChromosome11:222..5190 source=RiceJaponica11 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>ATTCCCACCACAACCAAGATGCCCTCTCCATGCCATTTGGTCTCTTCTCTCTCCCTCCTTGCAAATTGCATGACCCTCTCTCTCTCTCTCCACTTCTCTCTATAAACCTTCCTCTCTCCCATAACTTCTTTCCATTTTCAACCTACAAATATACTAATCTCTCTCTAGCTAGTCTTCACCTACAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCATGGAGACCACCACGCTTGTGCTGCTTCTTCCTCATGGCGGCGCCGGCGGCGTACGGCCGGCGGCAGCGGCAACGGCGAAGCGAAGCTACGTGATGAGGAGGTGTTGCTCGACGGTGAGGGCGGTCATGGCGAGGCCGCAAGAGGCGCCGGCGTCGGCGCCGGCCAAGAAGACGGAGACGGCGGCGATGATGTCGACGGTGCAGACGGAGACGGCGGCGGCGCCGCCGGCGACGGTGTACCGGGACAGCTGGTTCGACAAGCTCGCCATTGGTTACCTGTCCAGGAACCTTCAAGAAGCTTCTGGTTCGTGCCTATAATCTACTGAATATATCTGATGTATTGTGTGCATGTACATGCAATCTTTCGGATGAATTAATGAATGATCAAATCAACCATGCATGCATATATGTGTGTATTTATGATGTTTTTGCCATGGCTGATTTGATTTTTTGAGTGGAAGAAATAACATATATATGGTGAATAAACCAATGTTCTTCAGTTATTTTTGTGACAATTAAATTATATGTGTATTCTACGATCATCCTTTTCTTCTTATCGTTTGGTTATTAATACCCTTCTCGTCCATGTGGAACATAAATTCTTTGATTTTTTTTATTACATGTTGCTTTGGCCAGCGTGGGTGTACTTGCAATTGCATGCTTTCTGTCATATTATAATTGAATTAGCTCTTGATTTTTACATGCAAAAGAATAATTTATTTATTGAGTTGCTGATGATCACGTAAGCCTATGCATGTGAGATGTGATGTGTTCTCCGGCCTAATTAAGTATCGACGTACTATATATATGCACGTACATCAAGTACGGCCGTATTTCATAATTTGATTATTGTTACTGTACCAGACAAACCTAGTGCTTAATTATTGACATATACCCAACATCGATTAAAGTCAGTGTGACACAATTGGCAGAAGAAGATAACAAAAATCATAGAAGACATATATACCACAACAGTAAAAATTACAGTGCAAAAAGGGATACCAGAAGCTTCTTAATTAGGTGAATGGTCACATTTAGTAGTAGTAATACACAGTAATAACACATTATTTGACGAATAAATTAAGCGGAAGTGCATGATCAGCAGCATATAAGCAAGCAACTACGAGAGGTCAACCGTTGATCCGTGAAAATGTGAGGTTCACATTCTAGGAATCTTTTAGTGCTTGAATCTAATCCTTTCGTTTGTTTTCCCGTGGACTTTTTACTTTGATCGAATAAACTTATCTGAATAATGCGAAGGTCAAACATCACATGTACGTGCCCGGTTCTTCTTCTAGAAATTCAGCCGCTTACTATAATCGATGCTGGTTTTTGAATTTTTTTAAAGACATTTATTTTGGAACAACTTAAAAAAAGTGCACAAACCAGGGTTTACCAAACCGTTTGAAGTGAGGTTACTGCCACTCCATGGTTTGGCAATTATCACGAGGTGCGTCGTGGTTCCAAAAACTAATAAGGTTATCGTATGTGATAACCGCGATAACTGACGGTTTTGTAAACCCTGCACAGACAACATAATTATATTTACAGGACTGTTTGGACTTCATTAACACTGTAGCATCCACTACATCCGTCACCTCCTTGGCTCTCTTACATGTTTAGCAAAGTCAATCATACAGACAAACCAGAAAAGTTCCCAACAGACACAGAATGCTACTTTTCTAGTATGGGTAATTAAGCATATAAGAGAAACTTGAAAGCATACAGGAAACCGATGTTCACTAGTCCTGAGTTTTTTTTTGGTGTTGTTCAGGTGCAAATTAACTGCAAAGGTTGATTCCCTGAAAGGAACTTGTAGCACTTGCTGACTGACACGAACATGCATGCAATGTAATGCAGGGCTAAAGAATGAAAAGGATGGCTACGAGAGCCTGATAGATGCCGCCCTAGCCATCTCAAGAATCTTCAGTCTGGATAAACAAAGCGAGATTGTGACCCAAGCTCTTGAAAGAGCACTTCCAAGCTACATCCTCACAATGGTAAGTACCATAATCCATGACAATTGGCAATCATGTATGAATTATTGAAATTTAAAAAACCTAAAACTTTTTTTTATTTGAACAGGAAGTAAAATTCAGCATTTATTCTCTCTTTTTTTTTTGAGGAAAATACAGAACTTCTGTAAAGGGAGGAAACTTTAAAAGTTCTCTTCTTTTATTTCTTACCTCAACTTTGATTGAGAATTCTGTCGGCAGATCAAGGTGATGATGCCACCTTCAAGATTTTCCAGGGAGTACTTTGCTGCATTCACCACGATATTTTTTCCTTGGTTGGTTGGGCCGTGTGAGGTATATATTACATACACAGTTCCTCCTTTTGTTACTTCAGTTCAGAAAGGAATAGCTGCCTGATACCTGATTAGTGCGTCTTCACAAGAATGAATTCATGATTGTGCCTCTGCTGAAGGTAGCCCTGCAGAATGAATTGATACGAAGCCACACGTTAATTTGAGAAATATTGCTACAGAAGATCTTAAAATGCTGTTGAATGTAGGTTGCGACATATAATATCTCTTTGTTTTCAGGACATTACATTGTTTAAAGTAGTTGTACGAAGCATTTGTGGTGCAAATATCATATAAAGTATGGAATAAATGCCCTTGTATGGATAACTTGTCTTCTGAGTGATCATTCTGTCATTGAACAAAGGTTATGGAATCTGAAGTTGAAGGAAGGAAAGAGAAAAACGTGGTATATATCCCCAAATGCAGGTAATTCAATCATCATCAAGAAATTGTTTCACAAGTTTGACTAGGAGGAACATTTGTAAAGAAAATTTCTTCAACTGCTGGGTATCACGTAACATATCGGTCAAACGGTTTCCTACTCCCAGCTCTTCATAGGCAGACTACAAAAATTTTCAGGTCTATAAGTAGTAACCTGATGATTTGCAATTAACATATGACTCTGCAGCCATCAATTAGTTCTGAATGAATTGTTGCAGTATGTCAACTCTTACTCAAGTTAATCCACTCATGAAAAACAAATTGAAGAACTATGGCTGGGACACAAGTTGTATTGTCAACGTCGTAATTGTGGCAACTGACAATCCTACTCACACTCCACAAGCTGCTTATTATCAATTCTACCCACGTTAGACAAACTACCATGATTAACACTGGGTTAAGAAAAAAATAGAATTTTAAGTGCAGATATATCAATAAATACTGAAGAACCACCTTAAAAATACATAAATACTGAAGAACAGGATTTCTACTGGTGTTGCTGTTTTTCTTTTCTTTTTTTTTAATCTCCAATCCTATTTGTGGCAGATTTCTGGAAAGTACAAATTGTGTTGGTATGTGCACAAACCTTTGCAAGATTCCATGCCAGAAGTTCATCCAAGATTCACTTGGCATGAAGGTCTACATGTCTCCCAGTAAGCTTCCTCTGCTCTGATTCATCAGGAAAGCCTGAATATCAGTGGGTAAACACAACAACTTAAATATAATTTGGTAGAATAAGGTAAAAATAATCAAACTCCATTCCTTTGCATAAAGTTGGCAAGAAACTAATAATGGATTGTCATATCAACATCATCTCAAAGTTGAAAATTTGGGTTGAAACCTGGTGTGCCTCTAACTTCTCAGGACATTGACCTAATTTTACTATAGCGGATCATTATACGGACTGCCGCTAAAACATAAGTGAACTCAAAATATCAACTTTCGACTTTAAGCAGAATTAGTGAAGTAAAGTTGAATCAATGTAATAGGTCCATAGACCACCAACGGTTTAGAGCTATGAGAAATACAGAACTTAATTTGACATATTAATTTCATGCCACCTCTACCTACCGACAATCTGAGGACTGTGCCCTTTTTGGGTATGGGAACCACTGGCCACCATTCAGGAAACAGAAAGGGCTGAAGTGGGGCCAACATGTTTGCAGGTTACTACCTCCGTTTTTTAATTTATAACGTCGATGACTTTTTAGATATATATGATCATTCGTCTTATTCAAAAAAAAATGCAATTATCATTTATTTTATTGTGACTTAATTTATCATCAAATGTTCTTTAAGCATGACTTAAATTTTTTTTATATTTGCACAATAATTTTGAATAAGATGAATGGTCAAATGTTTGTCAAAAAGTCAACAACGTCATACATTAAAAAACGGAGGGAGTACAAAGCAATCACAAGCAAGTTGTACAGAAGAAGAAAGTGCTAGCCCCTACTTGTCATTCTTAGTGGGAAACGGCAAATTGCATGGACAAGTTGTGGAACAGTAGTGTGAAATCCAGAAATCCACAATGTAGTGCTGTGAAAATACAGTACAAGCACCTTCACATGGCAGTGACGAGCCAGCATGTCTCGATAATCAGAGCAATATGGTTCAATTTCACTTATGAGAAGCACTAGTTTTGAATGTTAGTCTTCTATGGTGATACACTGAATATTTCAACATCAATCATTTTTTCTAGGTCAAAAGATACTGATATTTTATGACATCACCCCACAGAATCAGATATCAACCTCTGTTGGAATTTGCAAGGCTCTCTTTCCTTTTTATCCTGTCGAGTGGTTAGATCTAGGAGTAAGCAATGAATGTTATTTACTGCCAGTGCTACTAAATAGTCCTATACAGCTTATTTCCAATTAAGCAAGAAGGTTTTTTCCAATACTAGGTCAATAAAAACGAGAATAAAGCAGAGTCCCTAAAGATAATAGTGAATGTATCAAGAGAAATGTAACATAATTACTTACATCAACTATATATTTATTCTTCAGATTTTGAAGACATGAGCTGTGAGATGATATTTGGACAGCAACCTCCTGAAGATGACCCTGCATTGAAGCAGCCATGCTTCCGGACAAAATGTAAGGAATTCCGCACTGAGGCACCTTCATGGATTCAATCAACACTCCTCTATATTCATACTTTTTACTTCGTGTATATGCAGGCGTCGCAAAGCAGAATCATGGTGTGAATTGCTCCATCTGATCTGAAAGAAATTATCAATAGATAGATTTCAAATCAGTAAAATGCCTTAAGCTCCATTTCCTTTATTCCTTTGGAAAAAAAATTAGCACCATCATTGTTTTTGCCCACAACACCAGCATGTTTGGAACATACACTCTTCATTGTAATCCAAAAGTAATCTAAGAGGAAATGAAAGGCCCAACAGTAACCATTTTAAGGTA</cdnaseq> |
| Protein Sequence |
<aaseq>METTTLVLLLPHGGAGGVRPAAAATAKRSYVMRRCCSTVRAVMARPQEAPASAPAKKTETAAMMSTVQTETAAAPPATVYRDSWFDKLAIGYLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQSEIVTQALERALPSYILTMIKVMMPPSRFSREYFAAFTTIFFPWLVGPCEVMESEVEGRKEKNVVYIPKCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCEMIFGQQPPEDDPALKQPCFRTKCVAKQNHGVNCSI</aaseq> |
| Gene Sequence |
<dnaseqindica>1..304#1861..2000#2217..2309#2670..2731#3293..3399#4756..4845#4929..4969#ATTCCCACCACAACCAAGATGCCCTCTCCATGCCATTTGGTCTCTTCTCTCTCCCTCCTTGCAAATTGCATGACCCTCTCTCTCTCTCTCCACTTCTCTCTATAAACCTTCCTCTCTCCCATAACTTCTTTCCATTTTCAACCTACAAATATACTAATCTCTCTCTAGCTAGTCTTCACCTACAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCATGGAGACCACCACGCTTGTGCTGCTTCTTCCTCATGGCGGCGCCGGCGGCGTACGGCCGGCGGCAGCGGCAACGGCGAAGCGAAGCTACGTGATGAGGAGGTGTTGCTCGACGGTGAGGGCGGTCATGGCGAGGCCGCAAGAGGCGCCGGCGTCGGCGCCGGCCAAGAAGACGGAGACGGCGGCGATGATGTCGACGGTGCAGACGGAGACGGCGGCGGCGCCGCCGGCGACGGTGTACCGGGACAGCTGGTTCGACAAGCTCGCCATTGGTTACCTGTCCAGGAACCTTCAAGAAGCTTCTGGTTCGTGCCTATAATCTACTGAATATATCTGATGTATTGTGTGCATGTACATGCAATCTTTCGGATGAATTAATGAATGATCAAATCAACCATGCATGCATATATGTGTGTATTTATGATGTTTTTGCCATGGCTGATTTGATTTTTTGAGTGGAAGAAATAACATATATATGGTGAATAAACCAATGTTCTTCAGTTATTTTTGTGACAATTAAATTATATGTGTATTCTACGATCATCCTTTTCTTCTTATCGTTTGGTTATTAATACCCTTCTCGTCCATGTGGAACATAAATTCTTTGATTTTTTTTATTACATGTTGCTTTGGCCAGCGTGGGTGTACTTGCAATTGCATGCTTTCTGTCATATTATAATTGAATTAGCTCTTGATTTTTACATGCAAAAGAATAATTTATTTATTGAGTTGCTGATGATCACGTAAGCCTATGCATGTGAGATGTGATGTGTTCTCCGGCCTAATTAAGTATCGACGTACTATATATATGCACGTACATCAAGTACGGCCGTATTTCATAATTTGATTATTGTTACTGTACCAGACAAACCTAGTGCTTAATTATTGACATATACCCAACATCGATTAAAGTCAGTGTGACACAATTGGCAGAAGAAGATAACAAAAATCATAGAAGACATATATACCACAACAGTAAAAATTACAGTGCAAAAAGGGATACCAGAAGCTTCTTAATTAGGTGAATGGTCACATTTAGTAGTAGTAATACACAGTAATAACACATTATTTGACGAATAAATTAAGCGGAAGTGCATGATCAGCAGCATATAAGCAAGCAACTACGAGAGGTCAACCGTTGATCCGTGAAAATGTGAGGTTCACATTCTAGGAATCTTTTAGTGCTTGAATCTAATCCTTTCGTTTGTTTTCCCGTGGACTTTTTACTTTGATCGAATAAACTTATCTGAATAATGCGAAGGTCAAACATCACATGTACGTGCCCGGTTCTTCTTCTAGAAATTCAGCCGCTTACTATAATCGATGCTGGTTTTTGAATTTTTTTAAAGACATTTATTTTGGAACAACTTAAAAAAAGTGCACAAACCAGGGTTTACCAAACCGTTTGAAGTGAGGTTACTGCCACTCCATGGTTTGGCAATTATCACGAGGTGCGTCGTGGTTCCAAAAACTAATAAGGTTATCGTATGTGATAACCGCGATAACTGACGGTTTTGTAAACCCTGCACAGACAACATAATTATATTTACAGGACTGTTTGGACTTCATTAACACTGTAGCATCCACTACATCCGTCACCTCCTTGGCTCTCTTACATGTTTAGCAAAGTCAATCATACAGACAAACCAGAAAAGTTCCCAACAGACACAGAATGCTACTTTTCTAGTATGGGTAATTAAGCATATAAGAGAAACTTGAAAGCATACAGGAAACCGATGTTCACTAGTCCTGAGTTTTTTTTTGGTGTTGTTCAGGTGCAAATTAACTGCAAAGGTTGATTCCCTGAAAGGAACTTGTAGCACTTGCTGACTGACACGAACATGCATGCAATGTAATGCAGGGCTAAAGAATGAAAAGGATGGCTACGAGAGCCTGATAGATGCCGCCCTAGCCATCTCAAGAATCTTCAGTCTGGATAAACAAAGCGAGATTGTGACCCAAGCTCTTGAAAGAGCACTTCCAAGCTACATCCTCACAATGGTAAGTACCATAATCCATGACAATTGGCAATCATGTATGAATTATTGAAATTTAAAAAACCTAAAACTTTTTTTTATTTGAACAGGAAGTAAAATTCAGCATTTATTCTCTCTTTTTTTTTTGAGGAAAATACAGAACTTCTGTAAAGGGAGGAAACTTTAAAAGTTCTCTTCTTTTATTTCTTACCTCAACTTTGATTGAGAATTCTGTCGGCAGATCAAGGTGATGATGCCACCTTCAAGATTTTCCAGGGAGTACTTTGCTGCATTCACCACGATATTTTTTCCTTGGTTGGTTGGGCCGTGTGAGGTATATATTACATACACAGTTCCTCCTTTTGTTACTTCAGTTCAGAAAGGAATAGCTGCCTGATACCTGATTAGTGCGTCTTCACAAGAATGAATTCATGATTGTGCCTCTGCTGAAGGTAGCCCTGCAGAATGAATTGATACGAAGCCACACGTTAATTTGAGAAATATTGCTACAGAAGATCTTAAAATGCTGTTGAATGTAGGTTGCGACATATAATATCTCTTTGTTTTCAGGACATTACATTGTTTAAAGTAGTTGTACGAAGCATTTGTGGTGCAAATATCATATAAAGTATGGAATAAATGCCCTTGTATGGATAACTTGTCTTCTGAGTGATCATTCTGTCATTGAACAAAGGTTATGGAATCTGAAGTTGAAGGAAGGAAAGAGAAAAACGTGGTATATATCCCCAAATGCAGGTAATTCAATCATCATCAAGAAATTGTTTCACAAGTTTGACTAGGAGGAACATTTGTAAAGAAAATTTCTTCAACTGCTGGGTATCACGTAACATATCGGTCAAACGGTTTCCTACTCCCAGCTCTTCATAGGCAGACTACAAAAATTTTCAGGTCTATAAGTAGTAACCTGATGATTTGCAATTAACATATGACTCTGCAGCCATCAATTAGTTCTGAATGAATTGTTGCAGTATGTCAACTCTTACTCAAGTTAATCCACTCATGAAAAACAAATTGAAGAACTATGGCTGGGACACAAGTTGTATTGTCAACGTCGTAATTGTGGCAACTGACAATCCTACTCACACTCCACAAGCTGCTTATTATCAATTCTACCCACGTTAGACAAACTACCATGATTAACACTGGGTTAAGAAAAAAATAGAATTTTAAGTGCAGATATATCAATAAATACTGAAGAACCACCTTAAAAATACATAAATACTGAAGAACAGGATTTCTACTGGTGTTGCTGTTTTTCTTTTCTTTTTTTTTAATCTCCAATCCTATTTGTGGCAGATTTCTGGAAAGTACAAATTGTGTTGGTATGTGCACAAACCTTTGCAAGATTCCATGCCAGAAGTTCATCCAAGATTCACTTGGCATGAAGGTCTACATGTCTCCCAGTAAGCTTCCTCTGCTCTGATTCATCAGGAAAGCCTGAATATCAGTGGGTAAACACAACAACTTAAATATAATTTGGTAGAATAAGGTAAAAATAATCAAACTCCATTCCTTTGCATAAAGTTGGCAAGAAACTAATAATGGATTGTCATATCAACATCATCTCAAAGTTGAAAATTTGGGTTGAAACCTGGTGTGCCTCTAACTTCTCAGGACATTGACCTAATTTTACTATAGCGGATCATTATACGGACTGCCGCTAAAACATAAGTGAACTCAAAATATCAACTTTCGACTTTAAGCAGAATTAGTGAAGTAAAGTTGAATCAATGTAATAGGTCCATAGACCACCAACGGTTTAGAGCTATGAGAAATACAGAACTTAATTTGACATATTAATTTCATGCCACCTCTACCTACCGACAATCTGAGGACTGTGCCCTTTTTGGGTATGGGAACCACTGGCCACCATTCAGGAAACAGAAAGGGCTGAAGTGGGGCCAACATGTTTGCAGGTTACTACCTCCGTTTTTTAATTTATAACGTCGATGACTTTTTAGATATATATGATCATTCGTCTTATTCAAAAAAAAATGCAATTATCATTTATTTTATTGTGACTTAATTTATCATCAAATGTTCTTTAAGCATGACTTAAATTTTTTTTATATTTGCACAATAATTTTGAATAAGATGAATGGTCAAATGTTTGTCAAAAAGTCAACAACGTCATACATTAAAAAACGGAGGGAGTACAAAGCAATCACAAGCAAGTTGTACAGAAGAAGAAAGTGCTAGCCCCTACTTGTCATTCTTAGTGGGAAACGGCAAATTGCATGGACAAGTTGTGGAACAGTAGTGTGAAATCCAGAAATCCACAATGTAGTGCTGTGAAAATACAGTACAAGCACCTTCACATGGCAGTGACGAGCCAGCATGTCTCGATAATCAGAGCAATATGGTTCAATTTCACTTATGAGAAGCACTAGTTTTGAATGTTAGTCTTCTATGGTGATACACTGAATATTTCAACATCAATCATTTTTTCTAGGTCAAAAGATACTGATATTTTATGACATCACCCCACAGAATCAGATATCAACCTCTGTTGGAATTTGCAAGGCTCTCTTTCCTTTTTATCCTGTCGAGTGGTTAGATCTAGGAGTAAGCAATGAATGTTATTTACTGCCAGTGCTACTAAATAGTCCTATACAGCTTATTTCCAATTAAGCAAGAAGGTTTTTTCCAATACTAGGTCAATAAAAACGAGAATAAAGCAGAGTCCCTAAAGATAATAGTGAATGTATCAAGAGAAATGTAACATAATTACTTACATCAACTATATATTTATTCTTCAGATTTTGAAGACATGAGCTGTGAGATGATATTTGGACAGCAACCTCCTGAAGATGACCCTGCATTGAAGCAGCCATGCTTCCGGACAAAATGTAAGGAATTCCGCACTGAGGCACCTTCATGGATTCAATCAACACTCCTCTATATTCATACTTTTTACTTCGTGTATATGCAGGCGTCGCAAAGCAGAATCATGGTGTGAATTGCTCCATCTGATCTGAAAGAAATTATCAATAGATAGATTTCAAATCAGTAAAATGCCTTAAGCTCCATTTCCTTTATTCCTTTGGAAAAAAAATTAGCACCATCATTGTTTTTGCCCACAACACCAGCATGTTTGGAACATACACTCTTCATTGTAATCCAAAAGTAATCTAAGAGGAAATGAAAGGCCCAACAGTAACCATTTTAAGGTA</dnaseqindica> 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| External Link(s) |
