Wbph8

WbpH8, short for glycosyltransferase8, is a kind of enzyme which catalyzes the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate.

Annotated Information

Function

WbpH8is a recessive gene associated with resistance to Whitebacked planthopper in rice.Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene. The whitebacked planthopper (WBPH), Sogatella furcifera, and brown planthopper (BPH) Nilaparvata lugens Stål are important sucking insects of rice (Oryza sativa L.) crops throughout the world. Rice 'B5', which has derived its resistance genes from the wild rice O. officinalis Wall ex Watt, is a line that is highly resistant to both WBPH and BPH. Previously, two resistance genes against BPH, Qbp1, and Qbp2 in 'B5' had been mapped onto chromosome 3 and chromosome 4, respectively.

A RFLP survey of the bulked extremes from the RIL population identified two genomic regions, one on chromosome 3 and the other on chromosome 4, likely containing the resistance genes to planthoppers. QTL analysis of the RILs further confirmed that two WBPH resistance genes were mapped on the same loci as Qbp1 and Qbp2, using a linkage map with 242 molecular markers distributed on 12 rice chromosomes. Of the two WBPH resistance genes, one designated Wbph7(t) was located within a 1.1-cM region between R1925 and G1318 on chromosome 3, the other designated Wbph8(t) was within a 0.3-cM region flanked by R288 and S11182 on chromosome 4. A two-way analysis of variance showed that two loci acted independently with each other in determining WBPH resistance. The results have significant implications in studying the interactions between sucking insects and plants and in breeding programs of resistance to rice planthoppers.

谭光轩等利用抗性水稻品系B5和感性水稻品种明恢63杂交，通过一粒遗传传递8代，得到一个含有187个稳定植株的重组自交系(recombinant inbred lines,RILs)群体。应用分离集团分析法，在第3和4染色体的2个区域筛选到与抗白背飞虱基因相连锁的分子标记。根据记录的重组自交系群体抗白背飞虱的表型资料，对具有242个分子标记的遗传连锁图的全基因组进行QTL扫描分析，把2个抗白背飞虱基因分别定位在抗褐飞虱基因Qbp1(Bph-14)和Qbp2(Bph-15)的相同位置上。其中，1个命名为Wbph8(t)的白背飞虱抗性基因被定位在第4染色体0.3-cM的区间内，两翼的分子标记是R288和S11182。

location

QTL analysis based on the data of the severity scores of RIL population confirmed the presence of the two WBPH resistance genes, and further determined their locations. One was mapped to a 1.1-cM length interval on the end of the long arm of chromosome 3, with an LOD score of 6.13, flanked by R1925 and G1318. This locus can explain 9.61% of the phenotypic variance of WBPH resistance in the population. We designated it as Wbph7(t). The other, designated as Wbph8(t), was resolved with an LOD score of 18.44 to a 0.3-cM interval between R288 and S11182 on the short arm close to the centromeric region of chromosome 4 . This locus accounted for 34.76% of the phenotypic variance of WBPH resistance in the population. The two QTLs jointly contributed 44.36% of the phenotypic variance of WBPH resistance in this population . Also, the same resistance loci against BPH were detected on chromosome 3 and 4 by whole genome scanning, individually explaining 14.79 and 28.77% of the total variation in the RIL population, and collectively 43.56%.

expression

Sequence: NC_002516.2 (3532812..3533933, complement) NC_002516.2

DNA SEQUENCE

gi|110645304:c3533933-3532812 Pseudomonas aeruginosa PAO1 chromosome, complete genome ATGACTAAAGTTGCTCATTTGACATCGGTTCACTCGCGTTATGATATTCGTATATTTCGAAAGCAGTGTA GAACACTCTCTCAATACGGATACGATGTGTATCTGGTTGTCGCAGATGGTAAGGGTGATGAAGTCAAGGA TGGTGTAAGGATTGTTGATGTCGGAGTACTCTCAGGTCGCTTGAATCGTATTCTAAAAACCACCCGAAAA ATTTATGAACAGGCTTTGGCGCTTGGGGCTGATGTCTATCATTTTCATGATCCCGAACTGATACCTGTTG GTCTTCGACTGAAAAAGCAAGGTAAGCAGGTTATCTTCGACTCCCATGAGGATGTGCCGAAGCAACTGCT GAGTAAACCTTACATGCGACCGTTTTTACGCCGTGTAGTGGCTGTGTTATTTTCCTGCTATGAGAAATAT GCATGCCCTAAGCTGGATGCAGTCCTTACGGCAACGCCGCATATTCGTGAAAAATTTAAAAATATTAATG GGAATGTTCTAGATATTAATAACTTTCCCATGTTGGGTGAGTTGGATGCGATGGTTCCTTGGGCAAGCAA GAAAACTGAAGTCTGCTACGTCGGTGGTATCACTTCCATTCGTGGTGTTCGTGAAGTCGTTAAGAGTCTT GAGTGCTTGAAGTCCTCGGCGCGCTTGAATTTAGTGGGAAAGTTTTCAGAGCCAGAGATAGAAAAAGAAG TCAGAGCGCTCAAGGGATGGAACTCCGTTAACGAACATGGTCAGCTTGATCGAGAAGATGTTCGTCGTGT ACTCGGTGACTCTGTTGCCGGGTTGGTGACATTTCTCCCAATGCCTAATCATGTTGATGCACAACCTAAT AAGATGTTCGAGTATATGTCGTCGGGAATCCCTGTGATCGCTTCCAATTTTCCTCTCTGGCGGGAAATTG TTGAAGGTAGCAATTGTGGTATATGCGTAGATCCTCTAAGTCCTGCTGCCATTGCTGAAGCGATCGACTA TCTGGTAAGTAATCCGTGTGAGGCGGCAGCGCTGGGACGTAATGGCCAGCGGGCAGTGAACGAACGTTAT AACTGGGATTTGGAAGGGCGCAAACTAGCGCGGTTCTATTCCGATCTACTGAGTAAGCGAGATTCCATAT GA

Labs working on this gene

Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of Chin

References

G X Tan1, Q M Weng1, X Ren1, Z Huang1, L L Zhu1 and G C He1.Two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance.Heredity (2004) 92, 212–217