IC4R008-miRNA-2011-21113019
Contents
Project Title
- Identification and analysis of seven H2O2-responsive miRNAs and 32 new miRNAs in the seedlings of rice (Oryza sativa L. ssp. indica)
The Background of This Project
- Plant microRNAs (miRNAs) have been shown to play critical roles in regulating gene expression at the post-transcriptional level. In this study, the researchers employed high throughput sequencing combined with computational analysis to survey miRNAomes from the seedlings of rice under normal conditions and treatments of H2O2 that result in oxidative stress.
Plant Culture & Treatment
- Rice (Oryza sativa L. ssp indica cv. 93-11) seedlings were grown in a growth chamber with 28/21℃ (16 h day/8 h night) and relative humidity of 70% as reported earlier (4). For H2O2 treatment, 12-day-old seedlings were treated with three H2O2 concentrations (0.6, 3.0 and 15.0mM) for 6 h in plastic containers, respectively (4). Seedlings immersed in dd H2O were used as a control. The rice seedlings were snap-frozen in liquid nitrogen, and then stored at -80℃ for RNA extraction.
Research Findings
Labs working on this Project
- Laboratory of Molecular Biology and MOE Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing 100084, P. R. China
Corresponding Author
- liujy@mail.tsinghua.edu.cn
