Os03g0236966
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Contents
Annotated Information
Function
Mutation
- The height of OsAPC6 mutant plants (∼80 cm.) and length of different internodes of tillers were reduced to half of that
of wild-type Basmati 370 (160 cm; Fig. 1a and b). Nearly, 40–45% seed sterility was observed in the OsAPC6 mutant with grain L/B ratio of 4.8 while in Basmati 370, it was 6.0 (Fig. 1c, d, and e). In the longitudinal section (LS) of the second internode, the length of cells in certain layers was reduced to half to that of the wild-type plants (SI, Fig. S1a and b). While the transverse section (TS; SI, Fig. S1c and d) had enhanced number of cell layers in the dwarf mutant (∼17) as compared to Basmati 370 (∼12). Similarly, in TS (SI, Fig. S1e and f), the number of cells in the leaf xylem was found to be more in the dwarf mutant as compared to Basmati 370. Reduction in cell size of OsAPC6 in the vascular bundles of fourth leaves was also observed in TS.
- Plant height of OsAPC6 mutant increased only 10% after exogenous gibberellic acid application which was not significantly different from the average plant height without GA3 application. This lack of response to GA3 indicated that the dwarf mutant OsAPC6 is gibberellic acid insensitive while all the cultivars with and without the sd1 dwarfing gene were GA3 sensitive.
Expression Pattern
Evolution
Subcellular localization
- In RT-PCR, a fragment of 350 bp was amplified using the cDNA-specific primers (DRTF and DRTR) in both roots and shoots (20 seedlings) of the wild-type Basmati 370, whereas no amplification was observed in the dwarf mutant (Fig. 4). The expression of wild-type gene in Basmati 370 was equal in both roots and shoots in 10-day-old seedlings. Lack of any amplification in the dwarf mutant OsAPC6 indicated the loss of expressions of wild-type gene due to T-DNA insertion.
Labs working on this gene
- National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India
References
- ↑ 1.0 1.1 Kumar M, Basha PO, Puri A, Rajpurohit D, Randhawa GS, Sharma TR, Dhaliwal HS. A candidate gene OsAPC6 of anaphase-promoting complex of rice identified through T-DNA insertion. Funct Integr Genomics. 2010 Aug;10(3):349-58. doi: 10.1007/s10142-009-0155-6. Epub 2010 Jan 21. PubMed PMID: 20091079.
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