Os02g0170300

The rice OsMADS29 is a member of MADS-box transcription factors in rice, it belongs to class B genes of the ABC model of flower development in plants.

Annotated Information

Function

Figure 1. Seeds from wild type lines Vs transgenic lines (from reference) .

OsMADS29 plays an important role during seed development of rice and silencing of OsMADS29 Results in the seeds shrunken as well as a reduced grain-filling rate in rice. It is a key regulator of the degradation of the nucellus and nucellar projection, which undergoing PCD during early rice seed development. Down regulation of OsMADS29 does not affect ovule development but causes seeds to abort or shrivel as they are most likely deficient in endosperm development and starch accumulation.
After rice fertilization, the increased IAA content induces the expression of MADS29, which then stimulates the degradation of the nucellus and the nucellar projection by directly stimulating a Cys protease and NBS-LRR proteins. Acting through MADS29 or other pathways, auxin regulates the PCDprocesses required for normal endosperm development and grain filling.

GO assignment(s): GO:0003700, GO:0005634, GO:0043565

Mutation

Figure 1 shows that during the first 6 days after flowering(DAF), there is no obvious difference between wide type line seed (ZH11) and A-MADS29 transgenic lines seeds (A-2, A-14, and A-33) in which the OsMADS29 was suppressed. After 8 DAF, the A-MADS29 seeds become shrunken, and this phenotype persists until 30 DAF because of a defect in dry matter accumulation, whereas the seeds are fully filled with dry matter by 30 DAF in ZH11.

Figure 2. Expression analyses of OsMADS29(from reference ).

Expression

OsMADS29 is Specifically Expressed in Ovules and Developing Seeds.

(1) RT-PCR analyses show that the expression of OsMADS29 initiates at panicles 0.1~5 cm, then increases gradually with the development of panicles at 6-22 cm. After pollination, the expression level reaches the maximum value at 5 DAP-7 DAP, then decreases from 9 DAP. No expression of OsMADS29 is detected in the vegetative organs (Figure 2A).
(2) Results of qRT-PCR show similar expression profiles of OsMADS29 to those of the RT-PCR (Figure 2B).
(3) RT-PCR was also performed to examine the expression of OsMADS29 in different floral organs. Like other known B(sister) genes, OsMADS29 is specifically expressed in the pistils (Figure 2C).

Evolution

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Labs working on this gene

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References

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Structured Information

Gene Name	Os02g0170300
Description	Similar to MADS box protein ZMM17
Version	NM_001052563.1 GI:115444496 GeneID:4328443
Length	4006 bp
Definition	Oryza sativa Japonica Group Os02g0170300, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 2
Location	Chromosome 2:3833127..3837132
Sequence Coding Region	3833796..3834074,3834202..3834243,3834352..3834393,3834894..3835055,3835193..3835262 ,3836519..3836706
Expression	GEO Profiles:Os02g0170300
Genome Context	<gbrowseImage1> name=NC_008395:3833127..3837132 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008395:3833127..3837132 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggggcgcggcaagatcgagatcaagaggatcgagaacgcgacgaacaggcaggtgacattctcgaagaggcggggagggctactgaagaaggcgaacgagctcgccgtgctctgcgacgcccgcgtcggcgtcgtcatcttctccagcaccggcaagatgttcgagtactgcagccctacctgcagtttgagggaactcatcgagcattaccagaccgtcaccaacactcatttcgaggagatcaaccacgatcagcaaatatttgtggagatgactcggatgaggaacgagatggagaagctggacggtggcatcaggaggttcaccggcgacgacctctccaacctcacgctcgccgacatcaacgatctcgagcagcagctcgaattctccgtcaccaaagtccgtgcgagaaagcatcagctcctgaaccagcagctggacaaccttcgtcgcaaggagcacatcctggaagaccagaacagcttcctgtgccgcatgatcaacgagaaccatcatcaggcggcggtgggcggcggcgacgtgaaggcgatggtggagatggcgccggtgctgtcgatgctgacggcggcgccggcgtactacggcgaggagtcgtcgagcaccgcgctgcagctcaccccgccgctgcacgccgtcgacgccgccgccgccgccgggttccggctgcagccgacgcagcccaacctgcaggaccccggctgcagcagcagcagcttccatgccgccgccgccggccacggcctgcagctgtggtaa</cdnaseq>
Protein Sequence	<aaseq>MGRGKIEIKRIENATNRQVTFSKRRGGLLKKANELAVLCDARVG VVIFSSTGKMFEYCSPTCSLRELIEHYQTVTNTHFEEINHDQQIFVEMTRMRNEMEKL DGGIRRFTGDDLSNLTLADINDLEQQLEFSVTKVRARKHQLLNQQLDNLRRKEHILED QNSFLCRMINENHHQAAVGGGDVKAMVEMAPVLSMLTAAPAYYGEESSSTALQLTPPL HAVDAAAAAGFRLQPTQPNLQDPGCSSSSFHAAAAGHGLQLW</aaseq>
Gene Sequence	<dnaseqindica>3059..3337#2890..2931#2740..2781#2078..2239#1871..1940#427..614#agcctttgaagcaagcttagcacttcacaaggtatgtacatagcacattactactcctgccctatctatctaccctgttcatgagtaattcaagaaaaaaaaaaggacaccttttctagctagttcttcatcctttcgcagcaaagagtttttacatctctagctactagttgttctcttctctacatgaaattttttctacttctgattgcaccctttacatagctgtggggggaaatccaatttgagcacccatgccttatgttgattttaacaaaattcgtccatgtatcgatatgttcataacttggccatttctgttcttcttcttcttcttcttcttcttcagtttcctcttgtgcagaagaaggagaaacaaaagaacacagaaattaaattaaactcgatcaggaaggtatagcaggcatggggcgcggcaagatcgagatcaagaggatcgagaacgcgacgaacaggcaggtgacattctcgaagaggcggggagggctactgaagaaggcgaacgagctcgccgtgctctgcgacgcccgcgtcggcgtcgtcatcttctccagcaccggcaagatgttcgagtactgcagccctacctgcaggtacggttcttgttttgttctcttatattatcagatgctgttcaatctttcctttctccagcttagtgtctcttgttctctttttagtgcttgatttgatgggttaattaatttcttgttttttgtcaaccaagagattaagttttcggctaagtttctctcggcctttattttgtttttcagagaaaattaactaactcatgcagtttctaccctttgttttctttgggcaagagtgtttaattatgatcatttgatctgttcatataatggatggatgccttgcatttattcttcatctgaactatttttcttgtcctgaactcctgatcgatcgagtttttcctaccttcattttaactattttcagtgtatatgtatgcatctgcagtttcctttgatatattttcgaagagccaagaatctattacaaaattaaatcatgatcaaaggcatacataagcaatgatctatatgccctagtgtctcttgtgacttgattacgcacttgtcttttacatctctgagcaacatatatgtatgtagatttgcaacttgatcctgaatttatagtcaactagttaattaatccttatttccctctttctaatgatttctaggatcgagctgggttttttaggtggatttccccaaaaaactatttcatatgtggctaatttaatcaaactaaaagatgtatatcaacagggaatataatttgaacaagtgtacgacagtacgaccatatcttatcttatgttatgcatgtttttatacttaaaaaaagatgcgaatataagagaatgacctaatattaaataattagaagaggtgaggtttcgaaccttggttgtctagtccaccactttgtggagctagccggaagatccccagacgtttctcacatgattttatactgttctgcagcatattatagttaattaatatgtgaatattcatgtgaagttctttcttccgatcccctgttcattcctgttattagttaagaaattttcgtttgtttcattttgatctaaaataatatactctctgtaagtatatacatcaccaagagaaaattctagctacaaaattaatttataccatctgatttgtacatcagagattgtcatttcttttttgaatgcgcaaaagaattacacatgcaccattccaattagatacatgaacttatcaaagtattaattaactcacaactaatcaaggtacttgtttcaatgtttgacaatatatagtttgagggaactcatcgagcattaccagaccgtcaccaacactcatttcgaggagatcaaccacgatcaggtatacattaattttacaatcagcaacacaatgtctgatcaatatcagaatatatatcgatctatctgccagcagttgaaatgaaattttctttcaaatgcactgatcaaatataattaatgtgcacatatatatagcaaatatttgtggagatgactcggatgaggaacgagatggagaagctggacggtggcatcaggaggttcaccggcgacgacctctccaacctcacgctcgccgacatcaacgatctcgagcagcagctcgaattctccgtcaccaaagtccgtgcgagaaaggtaattacttcctccatatcatattataagtcgttttaattttttttacaaatttatttaagtttaatcaagtttataaaaaatatattagtatttcaaaaacaaacattatcaaaatgtattcagtgatagatttaataaaattaatttggtattacatgtgttgctatttttttctataaatttaatcaaacttaaaagaaaaaaaattaaaatgacttgtaatatgaaatagagctagtaattgattaagaagatagtatttgatgaatgaatcattcttcagagttaatttagaatgatcagtctctgattcaatggtaaatggtaagtttagggtttagggattaattgatcccatagttttttcctcttttctgtaaagctttgctttcgtaagttttttattttgttatattcaatgaaataggtgtatctaagtgccgattcgttaaaaaaataaaaaatagttgtttcacattaattcttgcttgttgcagcatcagctcctgaaccagcagctggacaaccttcgtcgcaaggtaattaatttgcactactaaactgatcatgaaacaccaaatactttgctgtatgatgatgaaaaaaaaaatgattgattcaattctatgcatgaaattgatgaacaggagcacatcctggaagaccagaacagcttcctgtgccgcatggtatacgtatagttagttaatcagtaatcaagaattcattaaaatgttttgattcttgaagcacatatatgtacattaattatggtgtttggttgattcttgtacatgaaactatatatgaattcagatcaacgagaaccatcatcaggcggcggtgggcggcggcgacgtgaaggcgatggtggagatggcgccggtgctgtcgatgctgacggcggcgccggcgtactacggcgaggagtcgtcgagcaccgcgctgcagctcaccccgccgctgcacgccgtcgacgccgccgccgccgccgggttccggctgcagccgacgcagcccaacctgcaggaccccggctgcagcagcagcagcttccatgccgccgccgccggccacggcctgcagctgtggtaagaacaaacgaagcaaagcatacatggcacatcctgatactctcatcaggtgtgtgatgtgaatatatatatcgcaatgattttatctactagtttatgtacttcatccgttttataatataagtcattctagtatttttcatattcatattgatattaatgaatacatatatatatcaatataaatgtaggaaatgctagaataacttacattgtgaaacggagggagtataattcatcgcaacacagctgcgaattaatcatgcattgatatcattctgcagagatctagatgattgcttgctaattaagcacatgatggcattgtcgattctgggagctaggagtaacttggagaccatgaagatgcatgatcagctgatgatagacgatcgagttgggttgctgtgttgttcaattgttcatcgttctggacatatatatatatatatatgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtctaattagcacatcagaataatcaagcttttctacctagttaactagtacagctgctaagtaccttttgatttgttggtgtgtgaactactgaactggagttggatattaaggtctccatgcatttgcatggttaattagcagagagatcattgaggtttttaggatgtaac</dnaseqindica>
External Link(s)	NCBI Gene:Os02g0170300, RefSeq:Os02g0170300