Os10g0405500
The rice PAIR3 plays a crucial role in homologous chromosome pairing and synapsis in meiosis, which is important for eukaryotic sexual reproduction[1].
Contents
Annotated Information
Function
Meiosis is a highly conserved process in eukaryotes and occupies a central role in the life cycles of all sexually reproducing organisms[1]. During meiosis in rice, the gene PAIR3(HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS 3) was essential for bouquet formation, homologous pairing and normal recombination, and SC assembly [2].It is required for normal development of the resulting microspores and embryo sac formation[1].
Wild Type VS. Mutant
The researh by Kejian Wang et al. described the difference of chromosome behavior between PAIR3 mutants and wild type in 2011:
- In the wild type, chromosomes began to condense and form thin lines at leptotene(Figure 1A). In zygotene, pairing and synapsis occurred between homologous chromosomes(Figure 1B). The synapsis was completed, and 12 fully synapsed chromosomes were clearly visible at pachytene(Figure 1C). With further chromosome condensation occurring in diplotene and diakinesis, SCs were gradually disassembled and the paired homologous chromosomes were held together by chiasmata(Figure 1D). At metaphase I, all bivalents were aligned along the equatorial plate(Figure 1E). Subsequently, homologous chromosomes were separated from each other and moved to the opposite poles of the cell at anaphase I(Figure 1F). The second meiotic division was very similar to mitosis and finally produced tetrad spores(Figure 1, G–I)[2].
- In the PAIR3 mutant, there was no obvious difference during leptotene, compared with the wild type(Figure 2A ).However, pairing between homologous chromosomes was not observed from zygotene to pachytene (Figure 2, B and C ). During diakinesis and metaphase I, many randomly distributed univalents were found (Figure 2D ). In telophase I and prophase II, an uneven number of chromosomes could be seen in the two related cells (Figure 2, F and G ). After the second division, tetrads and polyads with different numbers of chromosomes were formed (Figure 2, H–L )[2].
Expression
RT-PCR analyses by Wenya Yuan et al. shows that PAIR3 was expressed at a very low level in vegetative organs(root, stem, shoots and leaf). It was preferentially expressed in the meiocytes, especially during the male and female meiosis stages[1].
Evolution
As PAIR3 shows no strong similarity with known proteins in Arabidopsis and other model organisms, it is likely to be a newly evolved meiotic protein in rice[1][2]. The only motif found in PAIR3 is coiled-coil domain[1].
Knowledge Extension
How haploid gametes are generated from diploid somatic cells is a interesting question in cytogenetics. Two important procedures are involed in it: Mitosis and Meiosis.
- The term mitosis was first introduced by Walther Flemming, who made detailed observations of chromosomes during cell division in Salamandra maculosa[3]. His results were published in 1878 and then in the seminal book Zellsubstanz, Kern und Zelltheilung (1882; Cell substance, nucleus and cell division)[3][4].During mitosis, one round of DNA replication is followed by a single round of chromosome segregation, thus generating two genetically identical daughter cells)[3].
- The term meiosis was coined by Farmer and Moore meaning reduction in chromosome number[5]. It is achieved by one round of DNA replication being followed by two rounds of chromosome segregation with no intervening round of DNA replication[3]. Fusion of two gametes during sexual reproduction restores the diploid complement of chromosomes in the zygote that gives rise to a new individual[3]. Meiosis was devided into several stages including Meiosis I, Leptotene, Zygotene, Pachytene, Diplotene, Diakinesis, Synchronous processes, Metaphase I, Anaphase I, Telophase I and Meiosis II[5].
Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China
- Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
- State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Reference
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Yuan W, Li X, Chang Y, et al. Mutation of the rice gene PAIR3 results in lack of bivalent formation in meiosis[J]. The Plant Journal, 2009, 59(2): 303-315.
- ↑ 2.0 2.1 2.2 2.3 2.4 Wang K, Wang M, Tang D, et al. PAIR3, an axis-associated protein, is essential for the recruitment of recombination elements onto meiotic chromosomes in rice[J]. Molecular biology of the cell, 2011, 22(1): 12-19.
- ↑ 3.0 3.1 3.2 3.3 3.4 Petronczki M, Siomos M F, Nasmyth K. Un menage a quatre: the molecular biology of chromosome segregation in meiosis[J]. Cell, 2003, 112(4): 423-440.
- ↑ Flemming W. Zur Kenntnis der Zelle in ihrer Teilung-Erscheinungen[J]. Schriften Naturwiss. Vereins Schl.-Holsk, 1878, 3(1): 26.
- ↑ 5.0 5.1 Wikipedia term(Meiosis), available at http://en.wikipedia.org/wiki/Meiosis. (Last update: 12 March 2013).
Structured Information
| Gene Name |
Os10g0405500 |
|---|---|
| Description |
Conserved hypothetical protein |
| Version |
NM_001071094.2 GI:297610454 GeneID:4348581 |
| Length |
6865 bp |
| Definition |
Oryza sativa Japonica Group Os10g0405500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:14199602..14206466 |
| Sequence Coding Region |
14199602..14199622,14200704..14202353,14202462..14202609,14203640..14203780,14203853..14203913 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:14199602..14206466 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:14199602..14206466 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggaattgcatatttgcctcgctacatcaagtgactattggagtttggccagcaatcaatatccatgtggtaaattccctaaggtatcagttggcgtcacaattccaaggacgagttctgtatcaagaggcagagatgctgctagtaccgctgcatttgagaagaacttgtctcagggaactgatggaagatctagacctcccaaaatggataatgcttcacttcaggtctctccagaagcagcaaaccacggcggatctgctaaagaggttcctaaacctgtccctgctaaggtttctgtatcacaacctgatgataatgcaattgagcaaacaggaaccttttcctttggaacaagaagagaacaggacagtcatcttgatcaattagataggccaccacttgtgagttcccaaggaaagcgtcaagtggaatcagctgataaaaacaagcccaacagtgaaatgctcaggatgaaactgtgggagatccttggtggtacttcacaaaacaaggaggctgttgcctcaccaaatcctgaagatattgagacgccatgccaacctaaaagtcaaattgccaatggaccatcttcaggaagacagaaggttttcacttcacctgttccatataatattaagacaccagctcagtttaatagtcaaacagcgaacaaaccatcctctgatccaattgagtcagactccgacagtcctcaagtagttgaagtaagacctattactcgttcgctggggcgcaagaaagaaccaacaggctccacacatcaggataagagcgggagtgcaaagaaaccattgtctactcatcgttctacacccaagcagaaaatattggacaatgtgtttgccttcaacgataaatgcacacctaaaacagtaggaaaatctgcaaatggtgaatctggcagcttgaggaatcttagaagcttgagtaggagggctaaagttgagccaaagaaggcacattgttcggacaggatttctcataagactacacaggatgatatggaaagaaaggtaccttctaaatatataccatcagagaaaaaaggtgagaaaacaaactccttttcttctttatcccgaacaggaaaaactgctgagagttgttctagaagccctaaaagggagagaagggtgaacacgatggctaatgttggggctcgaaagatgcagttatctgaaaatttactggtcaagactctaaatgatggtgaacacaagctctcttctcctcagcttacttcctttaagagcaagggaaaatgttcttctatatcgcctcaacagaaggagaatgataatacccacatccctgaagcttcagacagaacagcagcaagaaatagttttaactccacaccttctcctgctgctaatccatctcctgtactgaggaagtactcatgggaacatgatgagaatcctgcgataaatggtaaatctggacagaaggatgccagtccgttggcagacagattcagcgacatgccagatgattttgcaagtcctacttttgcagcaaacataaaaatatccccccacagaagtaaaatgctagacgatgacctatttagctccaaatatccaaaaggtgtgaacaggtcaagatcaacttcctttacctcggatccagaatcggagccattggacaaaatggagaaaaccaacgagttacctggcagtgaatctcctaactctcaggaggaaagacagaacagaaaacaaccacatctttcacccctttctcctattgagagtgaaggggctcaaatttctattccaagctttagaaaaggatataaatctcataaatggctttcagatgttgacagccctgataaatcttctattgagcatctgggccgaaaatcacatctaaaagagggtagaaagggcaaaaggcaattaacttcgccaacccattttgccacctctgggacgcaagaaacaatgtcagacaaagaaccagaaaaagtcccagaaaactacctaaccagggcttttgatcagttagtagtggtgctaggaaggttccaaaccaaaatcaagtctgaaacaaggaataaaagttctaagatacttgcagctactggagagataatacgccagcaccttgaaggggttgaggggcagatgcaggctgatgtggacaagctggtcaatgcaggaaaatctaaaaggaaacgtctagagtcaacatttgaagagcaacaagaaaagttaaggattcttcacgagaagttcaaggaggaggtcaaccagcagttgctcggttgcaagaactctgttgaggattttgaggcttaccatgcagaacttaagggagttgctgacaagcaaaaagcctcacacaagaagctccttcaaaatgctgagaagacagtcggcgctcagctgagcgacgcggaaaccaaaatcgctgaggtccagaagagggcacggaagaggatgaagggcctcaaatttgtgctcaaggagctcattgcagaaactgcagattgtaacgggagctaa</cdnaseq> |
| Protein Sequence |
<aaseq>MELHICLATSSDYWSLASNQYPCGKFPKVSVGVTIPRTSSVSRG RDAASTAAFEKNLSQGTDGRSRPPKMDNASLQVSPEAANHGGSAKEVPKPVPAKVSVS QPDDNAIEQTGTFSFGTRREQDSHLDQLDRPPLVSSQGKRQVESADKNKPNSEMLRMK LWEILGGTSQNKEAVASPNPEDIETPCQPKSQIANGPSSGRQKVFTSPVPYNIKTPAQ FNSQTANKPSSDPIESDSDSPQVVEVRPITRSLGRKKEPTGSTHQDKSGSAKKPLSTH RSTPKQKILDNVFAFNDKCTPKTVGKSANGESGSLRNLRSLSRRAKVEPKKAHCSDRI SHKTTQDDMERKVPSKYIPSEKKGEKTNSFSSLSRTGKTAESCSRSPKRERRVNTMAN VGARKMQLSENLLVKTLNDGEHKLSSPQLTSFKSKGKCSSISPQQKENDNTHIPEASD RTAARNSFNSTPSPAANPSPVLRKYSWEHDENPAINGKSGQKDASPLADRFSDMPDDF ASPTFAANIKISPHRSKMLDDDLFSSKYPKGVNRSRSTSFTSDPESEPLDKMEKTNEL PGSESPNSQEERQNRKQPHLSPLSPIESEGAQISIPSFRKGYKSHKWLSDVDSPDKSS IEHLGRKSHLKEGRKGKRQLTSPTHFATSGTQETMSDKEPEKVPENYLTRAFDQLVVV LGRFQTKIKSETRNKSSKILAATGEIIRQHLEGVEGQMQADVDKLVNAGKSKRKRLES TFEEQQEKLRILHEKFKEEVNQQLLGCKNSVEDFEAYHAELKGVADKQKASHKKLLQN AEKTVGAQLSDAETKIAEVQKRARKRMKGLKFVLKELIAETADCNGS</aaseq> |
| Gene Sequence |
<dnaseqindica>1..21#1103..2752#2861..3008#4039..4179#4252..4312#4405..4554#4675..4733#5252..5386#6132..6223#6314..6378#6850..6865#atggaattgcatatttgcctcgttcgtaaacctatcgtgtaggtttccgttggatgattggatcctgaattctgtccgattgtttttgtctctctaaattatggttatctgccgcaagtgttaggctatgttagtgtcgcattccgcagtatttttcacggagttgggttcttctagatagtgagtggagcctggattgtgtattatgtttgtggaagaagatctcttaatgctgagggcacatttaaattgctgatacacaatattggtttgatatgtttgtaatggactgttcctagaatttcactgggggtactaaattgacttgttggacatgcagtctatttaatttgagcctcagtgcagcagtacactgttgcagttatcaatcaaaagcaagttgttagcaatcaaatcatccagcttagaaatttaggtgtggtactgtggttatgtaatgcagctccaccaccactgtagtaatttaaagttgacagttgtagtacaacgatgcagttatttgttactacttttcaatgctagtttcttttccaagcgtgattgcaatattgccatgtgacaatcttttacatgtattgcctgctgaatcaggaaaaagaagtacagatgtatcactaattcacttatgctttggtaggctagttgaggatcagtgtcccttgcccccatgtcaatgcttatttgcggtgttttcgcccttgctgatacagctgtgcttctcacatgtgatggcagatttgaaatttcttcctataaccatgcttgataaagatgtgctttgcaagacctctgtattcatttgctaagcataattttgattctgaaagattgtctcagaccagtagaatcttgtcttatgcagttgtttttctattcaccaggtccaaccttctttagttaatttggtatactattatcccaaaagctgcacctatggaagttgagctgacgaacattcagaaagtgagtagctatttcctctgctaagctgctggatgctttcctttttcatgttatcctttcaccttcagatgttttttcattatagtattaaaaaagttagcatgttatacttttgtaggctacatcaagtgactattggagtttggccagcaatcaatatccatgtggtaaattccctaaggtatcagttggcgtcacaattccaaggacgagttctgtatcaagaggcagagatgctgctagtaccgctgcatttgagaagaacttgtctcagggaactgatggaagatctagacctcccaaaatggataatgcttcacttcaggtctctccagaagcagcaaaccacggcggatctgctaaagaggttcctaaacctgtccctgctaaggtttctgtatcacaacctgatgataatgcaattgagcaaacaggaaccttttcctttggaacaagaagagaacaggacagtcatcttgatcaattagataggccaccacttgtgagttcccaaggaaagcgtcaagtggaatcagctgataaaaacaagcccaacagtgaaatgctcaggatgaaactgtgggagatccttggtggtacttcacaaaacaaggaggctgttgcctcaccaaatcctgaagatattgagacgccatgccaacctaaaagtcaaattgccaatggaccatcttcaggaagacagaaggttttcacttcacctgttccatataatattaagacaccagctcagtttaatagtcaaacagcgaacaaaccatcctctgatccaattgagtcagactccgacagtcctcaagtagttgaagtaagacctattactcgttcgctggggcgcaagaaagaaccaacaggctccacacatcaggataagagcgggagtgcaaagaaaccattgtctactcatcgttctacacccaagcagaaaatattggacaatgtgtttgccttcaacgataaatgcacacctaaaacagtaggaaaatctgcaaatggtgaatctggcagcttgaggaatcttagaagcttgagtaggagggctaaagttgagccaaagaaggcacattgttcggacaggatttctcataagactacacaggatgatatggaaagaaaggtaccttctaaatatataccatcagagaaaaaaggtgagaaaacaaactccttttcttctttatcccgaacaggaaaaactgctgagagttgttctagaagccctaaaagggagagaagggtgaacacgatggctaatgttggggctcgaaagatgcagttatctgaaaatttactggtcaagactctaaatgatggtgaacacaagctctcttctcctcagcttacttcctttaagagcaagggaaaatgttcttctatatcgcctcaacagaaggagaatgataatacccacatccctgaagcttcagacagaacagcagcaagaaatagttttaactccacaccttctcctgctgctaatccatctcctgtactgaggaagtactcatgggaacatgatgagaatcctgcgataaatggtaaatctggacagaaggatgccagtccgttggcagacagattcagcgacatgccagatgattttgcaagtcctacttttgcagcaaacataaaaatatccccccacagaagtaaaatgctagacgatgacctatttagctccaaatatccaaaaggtgtgaacaggtcaagatcaacttcctttacctcggatccagaatcggagccattggtatgcttcaatctctaaagcagattgcaactttagtaaatggttctgtttatactctacgtagcagagcaattttatccgtttgacactaattgttctactgtccaggacaaaatggagaaaaccaacgagttacctggcagtgaatctcctaactctcaggaggaaagacagaacagaaaacaaccacatctttcacccctttctcctattgagagtgaaggggctcaaatttctattccaagctttagaaaaggtcagaacgccatcaatgccaattttctaggctctttattaaaaaggggtagacatgctcctgtcatgtttaatgcttccattctagactcaaaggtagccatgctaacttacagtgcactacccccttattctgcatgtggaccatacttattggacagaaatcttatgtatatgtaattatgtatttcaagcacctcttgaatttatcatttaggtcataggatcaacactggccataatccttataacatttgcataatctgtcagatcaatcctgttagtattggtgttcatgatatgtacacttattttattggtgcaaattttgacatcgttacttttggcctcataaacgctcgaagtttgactatttgaacattgaattctaactatactggcaagcatatttgcagggttgttagtggtgagattgcaaaaaagaaaaaaaatttctgtgtcacccacttcatttacttgacctttttatgacaattacacattttatgtgacatgataaatgagtatttgtgtttgcttttgctgtcattttcgattagtgctgccttactaagccgttagatgtcttccaggtaacctgctattgtaacttgtgttcactggcctgaccgcagaaaatttcctaaattcctttttgtggctgtatagttaggctcttcagattttcagctatgtcttgctgctaggtcacaacatttttttgcaactgaaggatcttgaaacttgaaagtgccaattcatgagagcatgccgtttgtgtgatagctgacaagtggcatagcagcaacaagtggcatattctgccatgtgcaagcatatccttagtttacatatatttcatcagaactgtttaccatgttaaattcgctattagtttagttattcgactgtattattttcagcctttcagaacattaattacttgagctaaagttctcattggagatgcacacatctatctatcttaaagttgcttattcatgccttgtcgctttgtttttcacatgcaggatataaatctcataaatggctttcagatgttgacagccctgataaatcttctattgagcatctgggccgaaaatcacatctaaaagagggtagaaagggcaaaaggcaattaacttcgccaacccattttgccacctctggtaacactgattactttactgttgtgcttgtgcattcatggttttcggtctgaccaatattttttttctcagggacgcaagaaacaatgtcagacaaagaaccagaaaaagtcccagaaaactacctaaccaggtttgacttaggtcattgtggcttaaatagtaggtacaaacatgattgctagtttgctacttggattaatttcatttcttgacttccaccagggcttttgatcagttagtagtggtgctaggaaggttccaaaccaaaatcaagtctgaaacaaggaataaaagttctaagatacttgcagctactggagagataatacgccagcaccttgaaggggttgaggggcagatgcaggctgatgtgtaagatcgagtgccagtgccacttagaaactgcctcttttcttctttatgcacatgcgattgtatataggccagatatgtactgcctctttcatgcatggcatttaactgtgatttcagggacaagctggtcaatgcaggaaaatctaaaaggaaacgtctagagtcaacatttgaaggtaaaactctaatcatgtcgatgcaaccattcataatgcaattaatctatgagggcatgatgtggcaatattttttatccaactattgtttgttgtaactgtaacattttttattctacgtatttggattattcatctcatgtaacacaaactcttaaatcttgattcatcaatacagagtcatgtttgaactctagatagaatgtttgaagtctactacaggataacaatgtaatctgcctgttcccttggtttggttggctagaatgacaagtaatttgaaatggagggaacattagtttgagaaaatctcatgcagtatttgtcttattctgcctgcaatcatctgatgtggcttggacgcttggtctttatctacttcgttattttgatctcgtattatatactgagttaaatctcaatatgcaactgtatataatgttacaacttctttttccttcatgccttgtttttcttggatgattttaactattcaactgatgtcttctggtttgcagagcaacaagaaaagttaaggattcttcacgagaagttcaaggaggaggtcaaccagcagttgctcggttgcaagaactctgttgaggattttgaggcttaccatgcagaacttaagggagttgctgacaagcaaagtaagttaaacttaatacatacactatctttatgaatgggactgaagcaattaaagatttcttaccaatgtacacctgaaaatcccataaaaataatgtacacctgaaaaaagtctctaaggttgcaaagccatgttaacattctatatactgagcctgatgaactattccctccatctcacaacctaggaggcaccaagtaagaaccaataaatgttgagatgaccataaatatgcctactaaatgacatgctagacacactctacctgttttacagctttaaattagggtataatagaaaagggtggaatatttacattaattatgaaaagaatgtatcccgaaaaggacgggcagtttttgtgaaatgtaggaagtatggttataagcatttccattggaattctctacttacaaattggtaacactacatgttatccagtcatttgtacttcaactctttcttagtatagttaaaatcacacgtttagttttcgtgaaaaaacctaacgaatttagttgaaaagaatgacagcccaatcttcgtatgaagtgagttttctgagaggttcgccggttcccctgcaatggcatacttgtccagtgacataactccagctttgatcctctcttagcactattaggatgcaagttatggtgtggttaacaggacaaccacttcaaggattctatttatactaccgttcactgacccatggtgtgtgctaatatttgcatcaacagaagcctcacacaagaagctccttcaaaatgctgagaagacagtcggcgctcagctgagcgacgcggaaaccaaaatcgctgaggtccagaaggtgagccaatgcaacttttaaaccatcctgataagtgataaccatatatctaccaaatgctcaatctgtttttgacacgttgcttttcagagggcacggaagaggatgaagggcctcaaatttgtgctcaaggagctcattgcagaaactgcagagtaacccaaccaaagttggttcactctgcacaagtacatagcaaaggtgtattgaccaggacgtacagtttcacttacaaataggctactgtttcatttacaaagggttaaaggagaatttacaaaagggtacaggagaatagtgtcagaatgcatataggtttcttaccagatgaacttttcacagcatacattcaaggattctattctttccattagcatacattcttcgcctagctatcagatagacatgcattgttaccggcgtgccagtttgaataatttacattcctgagaaccgacacattttctgtgtactgtaatcgtattttatgaaaattgaaaattcataagttggaacatctactgcaaagttgcgatgcaaatgctcattgtacttggtttggtggcaatgttctctccattatttcagctgtgaagaatgtgtaatgtgtacatatgtttctgcagttgtaacgggagctaa</dnaseqindica> |
| External Link(s) |
