File:Gene expression pattern of CAP1..jpg

Gene expression pattern of CAP1. A, RT-PCR analysis of CAP1 and ubiquitin in vegetative and reproductive tissues. Total RNA was extracted from leaf blades of 2-month-old plants (Le), roots of 2-week-old plants (Ro), lemmas/paleas (L/P), and pistils (Pi) from spikelets before anthesis, spikelets at the meiotic stage (Me; stages 7 and 8 in Zhang et al. [2011]), and anthers at the microspore (Mi; stage 10), bicellular (Bi; stage 11), and tricellular (Tr; stages 12 and 13) stages and subjected to RT-PCR analysis. Amplifications of CAP1 were performed with 25 (top) and 35 (middle) cycles; the ubiquitin gene was amplified with 25 cycles (bottom). B–G, In situ localization of CAP1 transcript within wild-type anther locules at microspore (B and E; stage 10), bicellular pollen (C and F; stage 11), and tricellular pollen (D and G; stage 13) stages. Probed with the CAP1 antisense (B–D) and sense (E–G) probes. BP, Bicellular pollen; Ep, epidermis; En, endothecium; MS, microspore; Ta, tapetum; TP, tricellular pollen. Bar = 50 µm.