Os11g0184900

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The OsNAC5 gene is a member of plant specific NAC family in rice that encodes a transcript factor to regulate stress responses.

Annotated Information

Function

Stress-responsive proteins OsNAC5 is a transcriptional activator induced by abiotic stress,including drought,cold and high salinity.By microarray analysis,OsNAC5 upregulates many stress-inducible genes,including OsLEA3( the late embryogenesis abundant gene) and Os06g0681200 in rice plant that overexpressed OsNAC5.By gel mobility shift assay demonstrated that this protein bind specifically to the NAC recognition sequence of the OsLEA3 promoter (-56 to -85) and enhance the expression of this stress-related gene. Moreover,OsNAC5-overexpressing plants did not retard growth under non-stressed conditions. Hence, OsNAC5 may be an effective gene to enhance the stress tolerance of rice without inviting growth defects . In addition to abiotic stress, expression of OsNAC5 was also responsive to ABA,MeJA and other plant hormones such as ethylene,IAA,SA and BR could also induce rapid upregulation of OsNAC5 .

OsNAC5 has been characterized as a novel senescence associated ABA-dependent NAC transcription factor . The promoter regions of OsNAC5 which contain a conserved ABRE sequences (ACGTG G/TC) are activated in response to ABA . It is possible that OsNAC5 protein regulates similar transporter genes in rice and that these genes are needed for effective Fe and Zn remobilization .

OsNAC5 involves in modulating several downstream functional genes (associated with accumulation of compatible solutes,Na+,H2O2 and malondialdehyde. By detecting some metabolic changes,such as greater amounts of Pro and soluble sugars, and less amounts of MDA and H2O2 accumulated in OsNAC5-overexpressing rice plants suggests that it can protect plants from dehydration and oxidative damage in response to abiotic stresses .

OsNAC5 is a metal homeostasis related gene. Final seed Fe, Zn and protein concentrations were positively correlated with high and early OsNAC5 expression level in flag leaves (the major source of remobilized metals for developing seeds)during panicle emergence stage.(Sperotto et al., 2009)[3].The increase expression of OsNAC5 in NH+4 (compared with NH4+:NO3-) demonstrates that the NAC domain is crucial to plant development .

Mutation

The knockdown lines (Ri5, Ri6) less tolerant to cold,drought and salt stress, while overexpression of OsNAC5 (OE1) rice seedings conferred greater tolerance to these abiotic stress .

Root-specific overexpression of OsNAC5 can significantly enlarge the root diameter in transgenic rice plants due to enlarged steles and aerenchymas at flowering stage,suggesting the importance of this phenotype for improving the drought tolerance and grain yield in the field .

Expression

Each OsNAC gene has a unique expression pattern. OsNAC5 is predominantly expressed in root and embryo . Surprisingly,later studies found that it expresses in leaves, roots, stems and flowers and higher expression in flag leaves, non-flag leaves panicles and Lower expression in stems and roots .

Evolution

Amino acid sequence analysis revealed that the NAC genes in plants can fall into several subfamilies, such as the NAM, ATAF, and OsNAC3 subfamilies.OsNAC5 and OsNAC6 represent ATAF subfamily in rice .

Knowledge Extension

Subcellular localization studies using OsNAC5-GFP fusion proteins shows that OsNAC5 is localized to the nucleus.Transactivation assays demonstrated that OsNAC5 is a transcriptional activator that its activation domain lies C-terminal region between aa 273 and 328 .

OsNAC5 interacts with other OsNACs including itself to form homodimers and heterodimers. It also suggests that NAC-domain plays complex roles in binding to DNA and proteins .

Labs working on this gene

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References

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Structured Information

Gene Name	Os11g0184900
Description	Similar to NAC-domain protein 5-7
Version	NM_001072451.1 GI:115484514 GeneID:4349963
Length	2507 bp
Definition	Oryza sativa Japonica Group Os11g0184900, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 11
Location	Chromosome 11:4282487..4284993
Sequence Coding Region	4283269..4283796,4284345..4284640,4284730..4284895
Expression	GEO Profiles:Os11g0184900
Genome Context	<gbrowseImage1> name=NC_008404:4282487..4284993 source=RiceChromosome11 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008404:4282487..4284993 source=RiceChromosome11 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggagtgcggtggtgcgctgcagctgccgccggggttcaggttccacccgacggacgacgagctggtgatgtactacctgtgccgcaagtgcggcggcctccccctcgccgccccggtgatcgccgaggtggacctctacaagttcaacccatgggatctccccgagagggcgatgggaggggagaaggagtggtacttcttctcgccgcgggaccggaagtacccgaacgggcagcggccgaacagggcggcggggacggggtactggaaggcgacgggggcggacaagccggtggggtcgccgcgggcggtggcgatcaagaaggcgctcgtcttctacgccgggaagccgcccaagggcgtcaagaccaactggatcatgcacgagtaccgcctcgccgacgtcgaccgctccgccgccgcccgcaagctctccaagtcctcccacaacgccctcaggttggatgattgggtgttgtgccgaatctacaacaagaagggagtgatcgagaggtacgacacggtggacgccggcgaggacgtgaagccggcggcggcggcggcggcggcgaagggtggtcgcatcggcggcggcggcggcgcggcggcgatgaaggtggagctctccgactatgggttctacgaccaggagccggagtcggagatgctgtgcttcgaccggtcggggtcggcggaccgcgactcgatgccgcggctgcacaccgactccagcgggtcggagcacgtgctgtcgccgtcgccgtcgccggacgacttccccggcggcggcgaccacgactacgccgagagccagcccagcggcggatgcggcgggtggcccggcgtcgactgggccgccgtcggcgacgatggcttcgtcatcgacagctccctcttcgagctgccctcgccggcggcgttctcccgcgccgccggcgacggcgccgccttcggcgacatgttcacgtacctgcagaagccgttctaa</cdnaseq>
Protein Sequence	<aaseq>MECGGALQLPPGFRFHPTDDELVMYYLCRKCGGLPLAAPVIAEV DLYKFNPWDLPERAMGGEKEWYFFSPRDRKYPNGQRPNRAAGTGYWKATGADKPVGSP RAVAIKKALVFYAGKPPKGVKTNWIMHEYRLADVDRSAAARKLSKSSHNALRLDDWVL CRIYNKKGVIERYDTVDAGEDVKPAAAAAAAKGGRIGGGGGAAAMKVELSDYGFYDQE PESEMLCFDRSGSADRDSMPRLHTDSSGSEHVLSPSPSPDDFPGGGDHDYAESQPSGG CGGWPGVDWAAVGDDGFVIDSSLFELPSPAAFSRAAGDGAAFGDMFTYLQKPF</aaseq>
Gene Sequence	<dnaseqindica>1198..1725#354..649#99..264#attccaaaactttcgaaagcttccattagcgactaatccagccttcgttaagggtgattagaatttgattaatttcaaggcctcagatcggagattgaatggagtgcggtggtgcgctgcagctgccgccggggttcaggttccacccgacggacgacgagctggtgatgtactacctgtgccgcaagtgcggcggcctccccctcgccgccccggtgatcgccgaggtggacctctacaagttcaacccatgggatctccccggtaagcaagagcaaaagcagatcgaatcgaatcgaggaaattgaagtcgaattcaaattcaattaattggggatttggaattgttgcagagagggcgatgggaggggagaaggagtggtacttcttctcgccgcgggaccggaagtacccgaacgggcagcggccgaacagggcggcggggacggggtactggaaggcgacgggggcggacaagccggtggggtcgccgcgggcggtggcgatcaagaaggcgctcgtcttctacgccgggaagccgcccaagggcgtcaagaccaactggatcatgcacgagtaccgcctcgccgacgtcgaccgctccgccgccgcccgcaagctctccaagtcctcccacaacgccctcagggttagtatttacttcttttaattacttacttctcctaatctaatcatctcttgctttcaatcatttctcctcaccaacgaatttacccccattttattttttaaaattctacaaggaaaaaaaacagaaaagaaattgccgtatactgtatttgttcatatagtggtctcaatctagagagctctaggaagatatgggcaaatcaatcatcagtagaggtttgataaaatgatttttatttaaaatttgagaaaagaatttgaaaggtgtgccaaattggcaagttgcagcacgcctgagttgtcgctttcgtagcatggctgtagccgctaggtataggctcggatggcgtcatggctcacgcacgcgcagatagagacaagcccgcgcccaacctttatcgaatttaacgtcacctcggaaaacgtaatcacaccgaatttatttaccaaaaactttttaccaccaccactcgcatgcattccacatcaatcttgatcactttgctaataatttcagttttttttttggttaatttttttttgcagttggatgattgggtgttgtgccgaatctacaacaagaagggagtgatcgagaggtacgacacggtggacgccggcgaggacgtgaagccggcggcggcggcggcggcggcgaagggtggtcgcatcggcggcggcggcggcgcggcggcgatgaaggtggagctctccgactatgggttctacgaccaggagccggagtcggagatgctgtgcttcgaccggtcggggtcggcggaccgcgactcgatgccgcggctgcacaccgactccagcgggtcggagcacgtgctgtcgccgtcgccgtcgccggacgacttccccggcggcggcgaccacgactacgccgagagccagcccagcggcggatgcggcgggtggcccggcgtcgactgggccgccgtcggcgacgatggcttcgtcatcgacagctccctcttcgagctgccctcgccggcggcgttctcccgcgccgccggcgacggcgccgccttcggcgacatgttcacgtacctgcagaagccgttctaattaacggaacgtgacgcctctgcaaacgctaattagctcactaaccactgtcaggtcgatcgtgtaattcttttaccagtctagggtacagccaaaaaaccaaaaattaaccgtgctcgatcgatcgatcgatccatggatcgatgatcgcctccaccggcagatcaaaatcgaagcaaaaaatcagggagaattcttggtacttccagtacctctcgtcgacaccacgtgtcgggttttctgcaccgcggatcctcgctggccgcatcgtaacggcgagcgaggttaaaaatagtggagaattcaagaacacatccctgatttttgtttctgccggatggggagtaccgtgagcatggtagaaatgactggtaaaattttcgttgatgataggtagccagagtcgatgtaaccagtcgattttttttttttttggttttagttgacatgccttcctgatagattcagaacaaacaatagagaagagaggagaaaaaaacacagaggagaaattcagagaccaaagttcaaagttttggaattttcaggggcgccagcagctgatggtattgtcagtcgtgtctaggttcgtagaggaaattaaaatgtagaagaacgggtacttcagttcacttcaggttcgttcgtgccaaacaggtctctgacactttagttaggttttgatttttttttctctttttttctggttttttgtcgtttggtaatgtaatgtaagggtgattgcgacatggtatttatgtgattagagtcgctttcagttg</dnaseqindica>
External Link(s)	NCBI Gene:Os11g0184900, RefSeq:Os11g0184900