Os01g0257300

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Please input one-sentence summary here.

Annotated Information

Function

OsRAA1 encodes a 12.0-kD protein that has 58% homology to the AtFPF1 (Flowering Promoting Factor 1) in Arabidopsis, which has not been reported as modulating root development yet.

Expression

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Evolution

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Labs working on this gene

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  • OsRAA1 Interaction Protein Screening
  • OsRAA1 Interacts with OsRPT4 in Vitro and in Vivo
  • Aberrant Mitosis Occurs in OsRAA1 Transgenic Rice and Transgenic Yeast Cells
  • Degradation of OsRAA1 Depends on the Proteasome
  • Two-Hybrid cDNA Library Construction and Screening
  • Protein Deletion Mutation and Yeast Plasmid Construction
  • Pull-Down Assay
  • Immunofluorescence of Rice Root Tips
  • Coimmunoprecipitation and Tris-Tricine Gel Electrophoresis
  • Colocalization of OsRAA1 and Tubulin in the BY-2 Cell Line
  • Transient Subcellular Localization
  • Determination of the Half-Life of OsRAA1
  • Protein Degradation Assay in Vitro
  • Morphological Examination of Transformed S. pombeCells

References

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  • Rice ROOT ARCHITECTURE ASSOCIATED1 Binds the Proteasome Subunit RPT4 and Is Degraded in a D-Box and Proteasome-Dependent Manner Plant Physiology, 2008, 148(2): 843-855
  • Overexpression of OsRAA1 Causes Pleiotropic Phenotypes in Transgenic Rice Plants, including Altered Leaf, Flower, and Root Development and Root Response to Gravity
 Plant Physiology, 2004, 135(3): 1502-1513

Structured Information

Gene Name

Os01g0257300

Description

Conserved hypothetical protein

Version

NM_001049166.1 GI:115435745 GeneID:4326229

Length

785 bp

Definition

Oryza sativa Japonica Group Os01g0257300, complete gene.

Source

Oryza sativa Japonica Group

 ORGANISM  Oryza sativa Japonica Group
           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
           clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome

Chromosome 1

Location

Chromosome 1:8585137..8585921

Sequence Coding Region

8585497..8585826

Expression

GEO Profiles:Os01g0257300

Genome Context

<gbrowseImage1> name=NC_008394:8585137..8585921 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1>

Gene Structure

<gbrowseImage2> name=NC_008394:8585137..8585921 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2>

Coding Sequence

<cdnaseq>atgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaa</cdnaseq>

Protein Sequence

<aaseq>MSGVWVFKNGVVRLVEKQQATAGTAVAGGRRKALVHTPSGQVVS SYAALEARLTALGWERYYEDPSLFQFHKRGSLDLISLPADFSAFSSVHMYDIVVKNRD SFRVVDA</aaseq>

Gene Sequence

<dnaseqindica>96..425#tccatccacccatagctatctctagctagcttgcacattcttcattcttcttgcaatcagagctagagaaaaagagtttgagagagatctaagagatgtcaggggtttgggtgttcaagaacggggtggtgagattggtggagaagcagcaggcgacggcggggacggcggtggcgggagggaggaggaaggcgctggtgcacacgccgagcgggcaggtggtgtcgtcgtacgcggcgctggaggcgcggctgacggcgctcgggtgggagcgctactacgaggacccctccctcttccagttccacaagcgtggctccctcgacctcatctccctccccgccgacttctccgccttctcctccgtccacatgtacgacatcgtcgtcaagaaccgcgactccttccgcgtcgtcgacgcctaaattgacctatgtataggctcgcatgcatgcaaggtagacgaccatccaccttgcatgcatgcaggctatcattctctcttcgtctccgtcttcgtctttcatctttgttgtggtgtgtgtgaatttattatacttcttatgactgtgtgtgtgactgtgtgagagttcatggagagtgatgagtagtggtatacatatgtgtcgtcgctgatcgatttggtttattaccttgtgggtgggtattaattatcagcagtgtgttatatcaattgcttacttgtatgtgtatcatgtacatgagtgagtgtggctgatgcatatatatagaggtctttaataattatgtactatatatttgtg</dnaseqindica>

External Link(s)

NCBI Gene:Os01g0257300, RefSeq:Os01g0257300