Os02g0203500
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Contents
Annotated Information
Function
1.Map-based cloning of the RLS1 gene revealed that it encodes a previously uncharacterized NB (nucleotide-binding site)-containing protein with an ARM (armadillo) domain at the carboxyl terminus.
2.Consistent with its involvement in leaf senescence, RLS1 was up-regulated during dark-induced leaf senescence and down-regulated by cytokinin.
3.Intriguingly, constitutive expression of RLS1 also slightly accelerated leaf senescence with decreased chlorophyll content in transgenic rice plants.
4. the RLS1 gene might be involved in PCD that affects specific cellular processes.
5. RLS1 Is necessary for proper Chloroplast degradation.
Expression
1.There is only one copy of the RLS1 gene in the rice genome. The RLS1 homolog genes with unknown function were only found in sorghum, grape, and black cottonwood by a BLAST search. The putative NB domain of RLS1 contains the conserved motifs found in a large number of proteins binding ATP or GTP, and shows a significant similarity to that of homologous proteins from sorghum, grape, and black cottonwood. Genetic studies have provided evidence that NB-containing R proteins in plant, such as RPS4, RPM1, can activate cell death during HR execution (Hofius et al., 2009).
2. RLS1 expression Is affected by dark-induced senescence and Cytokinin treatment. RLS1 was more closely related to the CC-NB-LRR proteins, Rx, I2, R3a (Moffett et al., 2002; Couch et al., 2006; Jia et al., 2010).
3. The expression profile of RLS1 was investigated in various rice tissues at the adult stage with the quantitative real-time PCR approach. The results showed that RLS1 transcripts accumulated constitutively during leaf development, from young to senescent leaves (Figure 1). In addition, RLS1 was constitutively but
weakly expressed in the root of seedling, panicle, and spikelet. The highest level of the RLS1 transcript was detected in mature leaves, consistent with the phenotype of rls1.
4. Overexpression of RLS1 displays a weak Phenotype of accelerated leaf senescence(figure2)图片7 7A
Evolution
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The putative NB domain of RLS1 contains the conserved motifs found in a large number of proteins binding ATP or GTP, and shows a significant similarity to that of homologous proteins from sorghum, grape, and black cottonwood. Moreover, it is also similar (23–30% similarity) to those of various NB-containing R proteins (Figure 3A). We then assessed the sequence diversity of the selected homologs, by generating a phylogenetic tree using the NB sequences (Figure 3B). 图片5A 5B
Labs working on this gene
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References
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Structured Information
| Gene Name |
Os02g0203500 |
|---|---|
| Description |
Disease resistance protein family protein |
| Version |
NM_001052774.2 GI:297598786 GeneID:4328665 |
| Length |
3672 bp |
| Definition |
Oryza sativa Japonica Group Os02g0203500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:5785295..5788966 |
| Sequence Coding Region |
5785295..5787370,5787723..5788797,5788881..5788966 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:5785295..5788966 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:5785295..5788966 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggttggtgatgtcgaggttgatgaaggaacgggaccggaggattcggtgttggagttggtggcggccaggagaacgcacgccattggtttgtttgaccggaagcgaggccatatggatgctgtgaatgttcttgcctcggcgacccagctggtgtccgcgatgctcaccgcggtcggcgcgctggagcaggcggctgccgacttcgccgaggctcccaggaggctccaggttcttgaggattttgtgtccgaccttgggctgttgatgcagcaatccaagcagaagcacgcgcacaagatgcacgcgccgcagctcgagcgtcagctccagagcctaggcaagctgatggaccagctccatgccaacatcacaaaggcgaggcgggtgttgaagaaaggcaaagggaagaagggcttggccagagttgtgtggagctcggtgacaggggaccctctgatgaagtatgttcagctgatcagggatgatctcaactggtggcttgagttacagaagctaacagagagtgtgggcaatgtcatagcgtctacagctaagagtacgccatccttggtgagagtcaagtcggagcatggctatccagtgtcaaagaagtgcagctatgtcagggagctgcttatcaatgatggtagtcatcgagttgtcctgattgtcgggttatctggtattgggaagtcgtgtcttgctcgacaaatagcttctgacccgcctggtaattttgtggatggtgcaattgagcttagttttgggcggtggtgcagtagagcggcatgcaatggtaatagggatgaataccacaagcgtcttgttcggaaaatatgtaaatttcttgtgcagattggttccatgactgtcaatgaggatgtgggcaaagatcttgaagatgtctgttacttgcttcagactgcattggtaggaaggagcatgctgatcctacttgatgatgtctgggagcaagacatagttgatcgcttcacaaacctatatgataatgattgccggtatcttgtgacaacgagggatgaggcaatttatgagattgcagaagctgaaaaggtggaaatatccaaagatgatatcaaagaaattggcaaggacattcttctctatcatagccttcttactgttgaagagctcccgcctgttgcgtacgacttgctggatcgttgcggccaccatccccttacagttgctgtcatgggtaaggctctcagaaaggagaccagagtcgaaaaatgggatagagcaatctccaacctttccacatatgcaacttgtgcacctggaccggtttcatatgtgaatgagaaagaagtggaaaccacattgaccatctttgggtcttttgagttcagcttagaagcaatgccagaaaattcaagaaggtttttcatggtgcttgcagctatctcatgggatgaacctgttccagaggcttgccttgaatccatgtggtcagcactcatgcaggacactttattccccctcgtggtctcgaaactagtagaaggctcactcattatcaaactggaagaccaatcgatgtatcatatgcatgacatggtttcgctttacctcgagagtaaaacagacaacgctgttcatactcttttgttcggttcatttcctgaatatgctgcattggtgtctccttggcttttcatttttggcaaagagagcgcaaaagagcgagctgaacagaagataagatcattattttctttgctagagttcatggagatagagattttactaggaagcaccacccaagctctcatggaatgcaaatccatatctgaatttgaggctagcaggcttcacttcagcaaaatacttagtcctcgaatagcggagttaatttctgtggggtcaacatctctcattgttactgtcaccaaatctattacagtaatctttttccaaggagattatgctaagcttgctcagtctcttgaaacagcaggttctgtggataaattaattcatgttcttcgtggctgtgaagattcttctactctagccaatgtttctactgttcttgccaagatatctgagcatgttgatgctacaactgctgatgaaattttggcaaccattcctatggaccaaattgcgaaattactctctcctgagaatgaagaatggcatgaaattgtgtttacaactctagcatctttgataaaggtgggaaagttaagggctgttgagacaatgatcgaatcaggaattgacaagaagcttcttgttcttctaggcagtggttctgagatctcacagcatcatgcgattatcatgctaaaaacattctgtgaacttggtgcgccacttcaggggtgcatgggacctggagcgttgactcatttgccttggcatgctcgtctcagtttggagagatttgttttatttgaccaaaatgtaactccatcaccaaagcctcaacaatcttttgagttgattcttcacaagatactgcacagagacaacaaagataacattgaggcgatccaaggtttattacctctcgctgagagggctaatgattcaagggtgcaagatctccttttgggaagcaatatgtccaatgggttggcactgctgctgcaacggagagacattgaaagcaaccaagtgaggtctcacactgcttttctggtgatgaaattagcctgcactggaggggaaccatatgtccataggttcttagaggctaacattgttcatgagctaattgacatgatgcagtgcaacatcaacgatctccaggattcagcatactatgcccttcaccagattatctttgcgaaggggggatcacttgtattacagagatttttacaagcaggaaccattgaaaaattggttaacttgcttgaccgcaagtcttcaaagacaaaggaactaacaatgcagcttctggtagacattgcggtggtcggaaccaaaccttgcatcgaaagaatgctctcgtcccaaatcatcgagaaatttgtggcactcgagaaagcaggtgggtctttcagtggagcggtatcaagatacgtgcaggggttgaacatgtgcaagaacgtccagtctgctgagagatcagtcatgaagcaacagattctgaggaaagtgagatcagagataagaggtcatgatctggaagcaagtctagttgcctctgtggaagcttgcatttctgaaaaaggagccagcagcaggaggaaaaagtag</cdnaseq> |
| Protein Sequence |
<aaseq>MVGDVEVDEGTGPEDSVLELVAARRTHAIGLFDRKRGHMDAVNV LASATQLVSAMLTAVGALEQAAADFAEAPRRLQVLEDFVSDLGLLMQQSKQKHAHKMH APQLERQLQSLGKLMDQLHANITKARRVLKKGKGKKGLARVVWSSVTGDPLMKYVQLI RDDLNWWLELQKLTESVGNVIASTAKSTPSLVRVKSEHGYPVSKKCSYVRELLINDGS HRVVLIVGLSGIGKSCLARQIASDPPGNFVDGAIELSFGRWCSRAACNGNRDEYHKRL VRKICKFLVQIGSMTVNEDVGKDLEDVCYLLQTALVGRSMLILLDDVWEQDIVDRFTN LYDNDCRYLVTTRDEAIYEIAEAEKVEISKDDIKEIGKDILLYHSLLTVEELPPVAYD LLDRCGHHPLTVAVMGKALRKETRVEKWDRAISNLSTYATCAPGPVSYVNEKEVETTL TIFGSFEFSLEAMPENSRRFFMVLAAISWDEPVPEACLESMWSALMQDTLFPLVVSKL VEGSLIIKLEDQSMYHMHDMVSLYLESKTDNAVHTLLFGSFPEYAALVSPWLFIFGKE SAKERAEQKIRSLFSLLEFMEIEILLGSTTQALMECKSISEFEASRLHFSKILSPRIA ELISVGSTSLIVTVTKSITVIFFQGDYAKLAQSLETAGSVDKLIHVLRGCEDSSTLAN VSTVLAKISEHVDATTADEILATIPMDQIAKLLSPENEEWHEIVFTTLASLIKVGKLR AVETMIESGIDKKLLVLLGSGSEISQHHAIIMLKTFCELGAPLQGCMGPGALTHLPWH ARLSLERFVLFDQNVTPSPKPQQSFELILHKILHRDNKDNIEAIQGLLPLAERANDSR VQDLLLGSNMSNGLALLLQRRDIESNQVRSHTAFLVMKLACTGGEPYVHRFLEANIVH ELIDMMQCNINDLQDSAYYALHQIIFAKGGSLVLQRFLQAGTIEKLVNLLDRKSSKTK ELTMQLLVDIAVVGTKPCIERMLSSQIIEKFVALEKAGGSFSGAVSRYVQGLNMCKNV QSAERSVMKQQILRKVRSEIRGHDLEASLVASVEACISEKGASSRRKK</aaseq> |
| Gene Sequence |
<dnaseqindica>1597..3672#170..1244#1..86#atggttggtgatgtcgaggttgatgaaggaacgggaccggaggattcggtgttggagttggtggcggccaggagaacgcacgccatgtgttcgtcggattgtcgcagccagctctgagtattgaattcttgctaggggagagattggagcttcgttgctgttgctctagtggtttgtttgaccggaagcgaggccatatggatgctgtgaatgttcttgcctcggcgacccagctggtgtccgcgatgctcaccgcggtcggcgcgctggagcaggcggctgccgacttcgccgaggctcccaggaggctccaggttcttgaggattttgtgtccgaccttgggctgttgatgcagcaatccaagcagaagcacgcgcacaagatgcacgcgccgcagctcgagcgtcagctccagagcctaggcaagctgatggaccagctccatgccaacatcacaaaggcgaggcgggtgttgaagaaaggcaaagggaagaagggcttggccagagttgtgtggagctcggtgacaggggaccctctgatgaagtatgttcagctgatcagggatgatctcaactggtggcttgagttacagaagctaacagagagtgtgggcaatgtcatagcgtctacagctaagagtacgccatccttggtgagagtcaagtcggagcatggctatccagtgtcaaagaagtgcagctatgtcagggagctgcttatcaatgatggtagtcatcgagttgtcctgattgtcgggttatctggtattgggaagtcgtgtcttgctcgacaaatagcttctgacccgcctggtaattttgtggatggtgcaattgagcttagttttgggcggtggtgcagtagagcggcatgcaatggtaatagggatgaataccacaagcgtcttgttcggaaaatatgtaaatttcttgtgcagattggttccatgactgtcaatgaggatgtgggcaaagatcttgaagatgtctgttacttgcttcagactgcattggtaggaaggagcatgctgatcctacttgatgatgtctgggagcaagacatagttgatcgcttcacaaacctatatgataatgattgccggtatcttgtgacaacgagggatgaggcaatttatgagattgcagaagctgaaaaggtggaaatatccaaagatgatatcaaagaaattggcaaggacattcttctctatcatagccttcttactgttgaagagctcccggtatactcttctatttgcatctttcatggttactgaatactcctgcactttagcctattttagttttacctgataaaattgttttgcaccctgcagctaaggtatcatcttcatgtggattgtatatgtgtttacatttcaacattggtttcgttttaatatccttcatgtttaacatggaaagtacacaaatatagttagccaactatttatattcatgttagtctacttaaagtaattagttatatgtgtatctgtcatacaagagatgttcaatatgtaacttagaacttagaactactaaagacctagttttatgggtttgtatggttaacattcctgttctttacagcctgttgcgtacgacttgctggatcgttgcggccaccatccccttacagttgctgtcatgggtaaggctctcagaaaggagaccagagtcgaaaaatgggatagagcaatctccaacctttccacatatgcaacttgtgcacctggaccggtttcatatgtgaatgagaaagaagtggaaaccacattgaccatctttgggtcttttgagttcagcttagaagcaatgccagaaaattcaagaaggtttttcatggtgcttgcagctatctcatgggatgaacctgttccagaggcttgccttgaatccatgtggtcagcactcatgcaggacactttattccccctcgtggtctcgaaactagtagaaggctcactcattatcaaactggaagaccaatcgatgtatcatatgcatgacatggtttcgctttacctcgagagtaaaacagacaacgctgttcatactcttttgttcggttcatttcctgaatatgctgcattggtgtctccttggcttttcatttttggcaaagagagcgcaaaagagcgagctgaacagaagataagatcattattttctttgctagagttcatggagatagagattttactaggaagcaccacccaagctctcatggaatgcaaatccatatctgaatttgaggctagcaggcttcacttcagcaaaatacttagtcctcgaatagcggagttaatttctgtggggtcaacatctctcattgttactgtcaccaaatctattacagtaatctttttccaaggagattatgctaagcttgctcagtctcttgaaacagcaggttctgtggataaattaattcatgttcttcgtggctgtgaagattcttctactctagccaatgtttctactgttcttgccaagatatctgagcatgttgatgctacaactgctgatgaaattttggcaaccattcctatggaccaaattgcgaaattactctctcctgagaatgaagaatggcatgaaattgtgtttacaactctagcatctttgataaaggtgggaaagttaagggctgttgagacaatgatcgaatcaggaattgacaagaagcttcttgttcttctaggcagtggttctgagatctcacagcatcatgcgattatcatgctaaaaacattctgtgaacttggtgcgccacttcaggggtgcatgggacctggagcgttgactcatttgccttggcatgctcgtctcagtttggagagatttgttttatttgaccaaaatgtaactccatcaccaaagcctcaacaatcttttgagttgattcttcacaagatactgcacagagacaacaaagataacattgaggcgatccaaggtttattacctctcgctgagagggctaatgattcaagggtgcaagatctccttttgggaagcaatatgtccaatgggttggcactgctgctgcaacggagagacattgaaagcaaccaagtgaggtctcacactgcttttctggtgatgaaattagcctgcactggaggggaaccatatgtccataggttcttagaggctaacattgttcatgagctaattgacatgatgcagtgcaacatcaacgatctccaggattcagcatactatgcccttcaccagattatctttgcgaaggggggatcacttgtattacagagatttttacaagcaggaaccattgaaaaattggttaacttgcttgaccgcaagtcttcaaagacaaaggaactaacaatgcagcttctggtagacattgcggtggtcggaaccaaaccttgcatcgaaagaatgctctcgtcccaaatcatcgagaaatttgtggcactcgagaaagcaggtgggtctttcagtggagcggtatcaagatacgtgcaggggttgaacatgtgcaagaacgtccagtctgctgagagatcagtcatgaagcaacagattctgaggaaagtgagatcagagataagaggtcatgatctggaagcaagtctagttgcctctgtggaagcttgcatttctgaaaaaggagccagcagcaggaggaaaaagtag</dnaseqindica> |
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