File:Fig 1 Rice importina1 supported nuclear import of NLS-GFP in vertebratein vitroassay system..png

.Rice importina1 supported nuclear import of NLS-GFP in vertebratein vitroassay system.A, mouse importinbalone does not dock (panels candd), whereas in combination with rice importina1, it does dock the T-GFP to the nuclear envelope (panels aandb). Such docking did not occur when Tm-GFP was used as substrate (panels e andf). B, rice importin a1 mediates translocation of T-GFP (panels a andb), but not Tm-GFP (panels eandf), into the nucleus in conjunction with mouse importinband Ran-GDP. The translocation did not occur in the absence of rice importin a1(panels candd). Panels a, c, and e, fluorescence images;panels b, d, and f, phase contrast images