File:Neighbour-joining phylogenetic trees showing the predicted relationship between Arabidopsis AtPINs and (a) dicot (Medicago, soybean and potato) and (b) monocot (rice and wheat) PINs.jpg

The alignment on which the tree is based was prepared using ClustalX 1.81. The phylograms were drawn using Tree-View 1.6.6 (http://darwin.zoology.gla.ac.uk/∼rpage/treeviewx/). Amino acid sequences for Arabidopsis were taken from http://www.Arabidopsis.org: AtPIN1, At1g73590; AtPIN2, At5g57090; AtPIN3, At1g70940; AtPIN4, At2g01420; AtPIN5, At5g16530; AtPIN6, At1g77110; AtPIN7, At1g23080; AtPIN8, At5g15100. Data used were predicted amino acid sequences based on EST clones. The full cDNA sequences for soybean, potato and wheat are unknown and therefore the EST from the 3′ (for dicots) or 5′-end (for monocots) was used. The 3′- or 5′-end of the EST was selected according to where more sequences were available in databases. The sequences were taken from TIGR (http://www.tigr.org). Each EST used in this analysis was at least 288 nt (96 aa). The first two letters of each sequence represent the organism: Gm, soybean (Glycine max); St, potato (Solanum tuberosum); Mt, Medicago truncatula; Os, rice (Oryza sativa); Ta, wheat (Triticum aestivum). Genes are named according to which cluster of the Arabidopsis PIN family they belong.