File:Figure 1 In situ RNA expression patterns of OsMADS1 compared with OsMGH3. (a–f) OsMADS1 profile in developing inflorescence branch primordia and spikelets. Silver grains reveal regions with expression.jpg

In situ RNA expression patterns of OsMADS1 compared with OsMGH3. (a–f) OsMADS1 profile in developing inflorescence branch primordia and spikelets. Silver grains reveal regions with expression. (a) Sense OsMADS1 RNA probe as a control. (b–f) Hybridizations with antisense RNA probes. (b) No transcripts in the very young branching panicle. (c) Near-uniform early expression in the incipient floret meristem. Expression is excluded from the glume primordia (green arrows). Red arrow indicates the future carpel anlagen. (d) Spikelet during an early stage of organ differentiation with OsMADS1 expression in the developing lemma (le)/palea (pa) and weak expression in the carpel (ca). (e, f) Spikelets showing expression in differentiated lemma (le), palea (pa) and carpels (ca). No expression in lodicules (lo) and glumes (gl). (g–l) Non-radioactive RNA in situ analysis of OsMGH3 RNAs in panicles and florets at stages similar to that shown for OsMADS1. (g) No OsMGH3 transcripts in the vegetative SAM (red arrow) or (h) branching panicles (black arrows). (i) Uniform RNA distribution in young spikelet primordia similar to OsMADS1 (compare c, i). Green arrow points to the glume, red arrows to the emerging lemma and palea and the blue arrow indicates the carpel anlagen. (j) Spikelet during organ emergence. OsMGH3 profile begins to deviates from OsMADS1. (k,l) Spikelets with differentiated organs. Greatly reduced expression in the near mature lemma (le) and palea (pa); continued lack of expression in the glumes (gl). Expression is maintained in the lodicules (lo), stamens (st) and carpel (ca). Scale bar is 100 lm in all panels.