Os03g0285800
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Annotated Information
Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating stress responses in eukaryotic organisms.However, little is known about the role of MAPKs in modulating the interaction of defense pathways activated by biotic and abiotic factors. In this study, we have isolated and functionally characterized a stress-responsive MAPK gene (OsMAPK5) from rice.OsMAPK5 is a single-copy gene but can generate at least two differentially spliced transcripts. TheOsMAPK5 gene, its protein, and kinase activity were inducible by abscisic acid as well as various biotic (pathogen infection)and abiotic (wounding, drought, salt, and cold) stresses. To determine its biological function, we generated and analyzedtransgenic rice plants with overexpression (using the 35S promoter of Cauliflower mosaic virus) or suppression (using double-stranded RNA interference [dsRNAi]) ofOsMAPK5. Interestingly, suppression of OsMAPK5 expression and its kinase activity resulted in the constitutive expression of pathogenesis-related (PR) genes such as PR1 and PR10 in the dsRNAitransgenic plants and significantly enhanced resistance to fungal ( Magnaporthe grisea) and bacterial (Burkholderia glumae)pathogens. However, these same dsRNAi lines had significant reductions in drought, salt, and cold tolerance. By contrast, overexpression lines exhibited increased OsMAPK5 kinase activity and increased tolerance to drought, salt, and cold stresses. These results strongly suggest that OsMAPK5 can positively regulate drought, salt, and cold tolerance and negatively Modulate PR gene expression and broad-spectrum disease resistance.
Bphi008a up-regulation and down-regulation were accompanied by different changes in transcription levels of OsMPK5, OsMPK12, OsMPK13, and OsMPK17 in transgenic plants. Immunoblot analysis also showed that the OsMPK5 protein level increased in overexpressing plants and decreased in RNA interference plants after BPH feeding. In transgenic lines, changes in the expression levels of several enzymes that are important components of the defenses against the BPH were also observed. Finally, yeast two-hybrid screening results showed that Bphi008a is able to interact with a b-ZIP transcription factor (OsbZIP60) and a RNA polymerase polypeptide (SDRP).
RAI1 encodes a putative basic helix–loop–helix transcription factor. A microarray analysis of cells transformed with an inducible RAI1 construct showed increased expression of PAL1 and OsWRKY19 genes after induction, suggesting that these genes are regulated by RAI1. This was confirmed using RAI1 T-DNA activation-tagged and RNA interference lines. The PAL1 and OsWRKY19 genes were also up-regulated by sphingolipid and chitin elicitors, and the RAI1 activationtagged plants had increased resistance to a rice blast fungus. These results indicated that RAI1 is involved in defense responses in rice. RAI1 interacted with OsMAPK3 and OsMAPK6 proteins in vivo and in vitro. Also, RAI1 was phosphorylated by OsMAPK3/6 and OsMKK4-dd in vitro. Overexpression of OsMAPK6 and/or OsMAPK3 together with OsMKK4-dd increased PAL1 and OsWRKY19 expression in rice protoplasts. Therefore, the regulation of PAL1 and OsWRKY19 expression by RAI1 could be controlled via an OsMKK4–OsMAPK3/6 cascade. Co-immunoprecipitation assays indicated that OsMAPK3 and OsRac1 occur in the same complex as OsMAPK6
Expression
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Evolution
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Structured Information
| Gene Name |
Os03g0285800 |
|---|---|
| Description |
MAP Kinase |
| Version |
NM_001056305.1 GI:115452338 GeneID:4332475 |
| Length |
2662 bp |
| Definition |
Oryza sativa Japonica Group Os03g0285800, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 3:9898386..9901047 |
| Sequence Coding Region |
9898577..9898749,9899280..9899463,9899548..9899880,9900012..9900149,9900264..9900393 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008396:9898386..9901047 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008396:9898386..9901047 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggacggggcgccggtggcggagttcaggccgacgatgacgcacggcggccggtacctgctctacgacatcttcgggaacaagttcgaggtgacgaacaagtaccagccgcccatcatgcccattggccgcggcgcctacgggatcgtctgctccgtgatgaactttgagacgagggagatggtggcgataaagaagatcgccaacgcgttcaacaacgacatggacgccaagcgcacgctccgggagatcaagctcctcaggcacctcgaccacgagaacatcataggcatcagggatgtgatcccgccgccgatccctcaggcgttcaacgacgtctacatcgccacggagctcatggacaccgacctccatcacatcatccgctccaaccaagaactgtcagaagagcactgccagtatttcctgtaccagatcctgcgggggctcaagtacatccactcggcgaacgtgatccaccgcgacctgaagccgagcaacctgctgctgaacgccaactgcgacctcaagatctgcgacttcgggctggcgcggccgtcgtcggagagcgacatgatgacggagtacgtggtcacccggtggtaccgcgcgccggagctgctgctcaactccaccgactactccgccgccatcgacgtctggtccgtcggctgcatcttcatggagctcatcaaccgccagccgctcttccccggcagggaccacatgcaccagatgcgcctcatcaccgaggtgatcgggacgccgacggacgacgagctggggttcatacggaacgaggacgcgaggaagtacatgaggcacctgccgcagtacccgcgccggacgttcgcgagcatgttcccgcgggtgcagcccgccgcgctcgacctcatcgagaggatgctcaccttcaacccgctgcagagaatcacagttgaggaggcgctcgatcatccttacctagagagattgcacgacatcgccgatgagcccatctgcctggagcccttctccttcgacttcgagcagaaggctctaaacgaggaccaaatgaagcagctgatcttcaacgaagcgatcgagatgaacccaaacatccggtactag</cdnaseq> |
| Protein Sequence |
<aaseq>MDGAPVAEFRPTMTHGGRYLLYDIFGNKFEVTNKYQPPIMPIGR GAYGIVCSVMNFETREMVAIKKIANAFNNDMDAKRTLREIKLLRHLDHENIIGIRDVI PPPIPQAFNDVYIATELMDTDLHHIIRSNQELSEEHCQYFLYQILRGLKYIHSANVIH RDLKPSNLLLNANCDLKICDFGLARPSSESDMMTEYVVTRWYRAPELLLNSTDYSAAI DVWSVGCIFMELINRQPLFPGRDHMHQMRLITEVIGTPTDDELGFIRNEDARKYMRHL PQYPRRTFASMFPRVQPAALDLIERMLTFNPLQRITVEEALDHPYLERLHDIADEPIC LEPFSFDFEQKALNEDQMKQLIFNEAIEMNPNIRY</aaseq> |
| Gene Sequence |
<dnaseqindica>2299..2471#1585..1768#1168..1500#899..1036#655..784#189..340#agcgatcacaaattgtgccaattcacaaaccgccgcccccttccctttttaatagctgccttcgcctctcgtcccctctccctcatcgccttgctgtctctgcgaatcgagagagagtcagataaggtcgttaattaggtttgtcaattcggctgcttgcggcgagagaagaggaggagggattagggatggacggggcgccggtggcggagttcaggccgacgatgacgcacggcggccggtacctgctctacgacatcttcgggaacaagttcgaggtgacgaacaagtaccagccgcccatcatgcccattggccgcggcgcctacgggatcgtctggtacgcacacgccgattcctcttttccctgcctgccctagatttcttgccatgttaatcgatccttcgtcagttccttttgattgattgatacgcgtcgatgagtggttgagaggatggagtaattgggaccaattactaatcccctgtttttagagcagggatgattaatttgattctgcagctgagctgagcaggtttttagttagttattatcagcgtagtggttgactggttggcatgtctattcggagtactttttggtgctttgtttttctaatttctcttctgaattccgtgtgctggtgcctacagctccgtgatgaactttgagacgagggagatggtggcgataaagaagatcgccaacgcgttcaacaacgacatggacgccaagcgcacgctccgggagatcaagctcctcaggcacctcgaccacgagaacgtaagctcctattaggctataactagtagtggaactgaagactacgtggttaattacgcagcggcgtgtacctgaatttggtaatctgtcactaactgctacaacaacacgcagatcataggcatcagggatgtgatcccgccgccgatccctcaggcgttcaacgacgtctacatcgccacggagctcatggacaccgacctccatcacatcatccgctccaaccaagaactgtcagaagagcactgccaggtgagtgaactgtgagcgaatgattttttttttcatatatcgatcaatcacacaatcaatcgatagctgcagaaacatcaattcgatcttcctcatgtcacgtatgtatgattgcggccgtgttgttgcagtatttcctgtaccagatcctgcgggggctcaagtacatccactcggcgaacgtgatccaccgcgacctgaagccgagcaacctgctgctgaacgccaactgcgacctcaagatctgcgacttcgggctggcgcggccgtcgtcggagagcgacatgatgacggagtacgtggtcacccggtggtaccgcgcgccggagctgctgctcaactccaccgactactccgccgccatcgacgtctggtccgtcggctgcatcttcatggagctcatcaaccgccagccgctcttccccggcagggaccacatgcaccagatgcgcctcatcaccgaggtgagtgaattaatcagcgtagacactcacacgctgaattatgctacctgttcttgggtctcatcgtgttgggtccatgatcaggtgatcgggacgccgacggacgacgagctggggttcatacggaacgaggacgcgaggaagtacatgaggcacctgccgcagtacccgcgccggacgttcgcgagcatgttcccgcgggtgcagcccgccgcgctcgacctcatcgagaggatgctcaccttcaacccgctgcagagaatcacaggtgcgtgcagagggctaacccaactcgtgcttgctgctctgcttctaaactactaaaattagtacccaaaattacaagtactaatttactagctgagtgtgtactgtgtacagtaccctgttcagtatgctactgcatgtgtgctagtatagcactagtattttctatcgcactgcttctttgactggcgtatatagccgccggtgtttgacccatccccaactatttttatgtttacttgccgtgaacagtcggtctcttgatctcgtcactttaaagggaacccgtcttttagctgtctttggggctaccaacgattttgcatgttgctcaagtgacgataaaagactgtgttactttactgtgtagtagtgcagaagtagaataggtagcacgtttgcagcctctgtcggttagagttcagtagaccttagttttgaaacactagacaatagcagagtggtttagaaggatgacagaagatttgtgcttaagaactatatctcactgacatcgttcttgacatgcagttgaggaggcgctcgatcatccttacctagagagattgcacgacatcgccgatgagcccatctgcctggagcccttctccttcgacttcgagcagaaggctctaaacgaggaccaaatgaagcagctgatcttcaacgaagcgatcgagatgaacccaaacatccggtactagattgaatcaccatggaaatgagatcccgtctatacctgctttgtacatatgatcaagattgagagccgggtagactgaacattgcatttgtttgtttgttgatgttcgaaacccacattctctgcaagttgtggctgctttgtatgatatatggtactatgttcgaataaaagggtttggaactttggatt</dnaseqindica> |
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