Os10g0167600
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Contents
Annotated Information
Gene:OsCPDP; qUVR-10 Protein： Cyclobutane pyrimidine dimer (CPD) photolyase
Function
Repair of CPDs is essential for plant survival while exposed to UV-B-containing sunlight. Nuclear repair of the UV-B-induced CPDs involves the photoreversal of CPDs, photoreactivation, which is mediated by CPD photolyase that monomerizes the CPDs in DNA by using the energy of near-UV and visible light (300–500 nm).There are two kinds of CPD pthotolyase existed in rice leaves. One is 54kD and another is 56kD protein. The 56-kD native rice CPD photolyase was phosphorylated, which has significantly higher CPD photorepair activity. The native rice CPD photolyase possesses both a pterin-like chromophore and an FAD chromophore. Single amino acid alteration from glutamine to arginine leads to a deficit of CPD photolyase activity and that CPD photolyase activity is one of the main factors determining UVB sensitivity in rice.
Expression
Full-length CPD photolyase is encoded by a single gene, not a splice variant, and is expressed and targeted not only to nuclei but also to chloroplasts and mitochondria. Over-expressing CPD photolyase enhances thein vitro photorepair ability of rice. CPD photolyase-over-expressing plants are significantly more resistant to the growth-impairing effects of UVB radiation than the WT plant.
Evolution
Rice may have evolved a CPD photolyase that functions in chloroplasts, mitochondria and nuclei, and that contains DNA to protect cells from the harmful effects of UV-B radiation. The amino acid at position 126 of the deduced amino acid sequence of CPD photolyase in cultivars including such as Norin 1 was found to be arginine, the CPD photolyase activities of which were lower. The amino acid at that position in cultivars including such as Sasanishiki was glutamine, which has higher CPD photolyase activities.
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Labs working on this gene
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan,
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
Applied Genomics Laboratory, Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602 Japan,
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
References
1. Masaaki Takahashi;Mika Teranishi;Hiroyuki Ishida;Junji Kawasaki;Atsuko Takeuchi;Tomoyuki Yamaya;Masao Watanabe;Amane Makino;Jun Hidema
Cyclobutane pyrimidine dimer (CPD) photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA The Plant Journal, 2011, 66(3): 433-442
2. Mika Teranishi;Kentaro Nakamura;Hiroshi Morioka;Kazuo Yamamoto;Jun Hidema
The Native Cyclobutane Pyrimidine Dimer Photolyase of Rice Is Phosphorylated Plant Physiology, 2008, 146(4): 1941-1951
3. Jun Hidema;Taku Taguchi;Taiichi Ono;Mika Teranishi;Kazuo Yamamoto;Tadashi Kumagai
Increase in CPD photolyase activity functions effectively to prevent growth inhibition caused by UVB radiation The Plant Journal, 2007, 50(1): 70-79
4. Jun Hidema;Mika Teranishi;Yutaka Iwamatsu;Tokuhisa Hirouchi;Tadamasa Ueda;Tadashi Sato;Benjamin Burr;Betsy M. Sutherland;Kazuo Yamamoto;Tadashi Kumagai
Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in rice The Plant Journal, 2005, 43(1): 57-67
5. Tadamasa Ueda;Tadashi Sato;Jun Hidema;Tokuhisa Hirouchi;Kazuo Yamamoto;Tadashi Kumagai;Masahiro Yano
qUVR-10, a Major Quantitative Trait Locus for Ultraviolet-B Resistance in Rice, Encodes Cyclobutane Pyrimidine Dimer Photolyase Genetics, 2005, 171(4): 1941-1950
6. Mika Teranishi;Yutaka Iwamatsu;Jun Hidema;Tadashi Kumagai
Ultraviolet-B Sensitivities in Japanese Lowland Rice Cultivars: Cyclobutane Pyrimidine Dimer Photolyase Activity and Gene Mutation Plant and Cell Physiology, 2004, 45(12): 1848-1856
7. Jun Hidema;Tadashi Kumagai;Betsy M. Sutherland
UV Radiation–Sensitive Norin 1 Rice Contains Defective Cyclobutane Pyrimidine Dimer Photolyase The Plant Cell, 2000, 12(9): 1569-1578
Structured Information
| Gene Name |
Os10g0167600 |
|---|---|
| Description |
Similar to CPD photolyase |
| Version |
NM_001189112.1 GI:297727354 GeneID:9272017 |
| Length |
3900 bp |
| Definition |
Oryza sativa Japonica Group Os10g0167600, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:4343782..4347681 |
| Sequence Coding Region |
4343990..4344169,4344287..4344358,4344993..4345074,4345697..4345755,4345844..4345880 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:4343782..4347681 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:4343782..4347681 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgctgctgataaaagagagcatatctatacgtgagagggaacagcttgagaatgccaaaacacatgatcctttgtggaatgcatcgcagttggagatggttcaccatggaaaaatgcatggattcatgagaatgtactgggccaaaaagattctagaatggactagtggaccagaagaagcactttcaactgcaatttatttaaatgacaagtatgagatagatggcagggaccccagtggttacgtcggatgtatgtggtccatatgtggcctccatgatcagggttggaaggagcgtccagtatttggaaagatacgttacatgaattacgctggctgcaagagaaaattcgatgttgacgcttacatttcttatgtcaagagattagctggtcaatccaagaagaggaacgctgaggagtctccaaatcctgtagtcaagctttccaagtctcagcactaa</cdnaseq> |
| Protein Sequence |
<aaseq>MLLIKESISIREREQLENAKTHDPLWNASQLEMVHHGKMHGFMR MYWAKKILEWTSGPEEALSTAIYLNDKYEIDGRDPSGYVGCMWSICGLHDQGWKERPV FGKIRYMNYAGCKRKFDVDAYISYVKRLAGQSKKRNAEESPNPVVKLSKSQH</aaseq> |
| Gene Sequence |
<dnaseqindica>3513..3692#3324..3395#2608..2689#1927..1985#1802..1838#1647..1681#aattcacacccacaaacgcacgcccgcacgcgcgcgcgcgcgctcagagacctccgtcgccgcccgccgcacgccggcgacccaccgcgccgcacgatgccgccgacctcagtgagcccaccaagaacagcgccggggccggcgaacccgagcccggcacacccgtcccgcgtgcgggtgatccacccgggcggcgggaagcccggtgggccggtggtgtactggatgctgcgggaccagcggctcgccgacaactgggcgctcctccacgccgccggcctcgccgccgcctccgcgtccccgctcgccgtcgcgttcgcgctgttcccgaggcccttcctcctctccgcccgccgccgccagctcgggttcctcctccgcggcctccgccgcctcgccgccgacgccgccgcccgccacctccccttcttcctcttcaccggtacgcgcccttttctttttctttttctttttctttttcttttgtctccatccgtgacgatgacatgtgggccaccccgtcttgcgaaatgtgacgtggctttttctctggctcgctgacaggtgggcccgcggagatcccggcgctggtgcggcgccttggtgcgtcgacgctggtcgccgacttctcgccgctgcggccggtgagggaggcgctcgacgcggtggtcggcgacctgcggcgggaggcgcccggcgtggccgtgcaccaggtgagcgtggtggcattgctgtttgcatcaatcttccaagaaagttgggaatctttttggggatggataatgaattcgccggagaatttagagattcttgtggaattcttcgaatgcaggtggacgcgcacaacgtggtgcctgtgtggacggcgtcggcgaagatggagtattcagccaagaccttcagaggcaaggtgagcaaggtgatggatgagtaccttgtggagttccctgaattgccggcggtggtgccatgggacagggagcagccggagggggtcgactgggacgcactcatcgccagggtttgcaggtgcggatgataattctaaggtgtattgggtggtaattttgtttgtgtagatatggatgactattgattcttggttgtttgagcagtgaggcggagaatgtgccggagattgactggtgtgagcctggagaggaagcagccatagaggcgcttctcggcagcaaggatggattcctgacgaagaggatcaagagctatgaaactgaccggaatgatcccacgaaaccacgggcattgtctgggctttcaccataccttcattttgggcacatttcggcacagcggtgtgcgctcgaggcaaagaaatgccgacatcttagtcccaaggtgctgttctttaactgatttgttatgattaaaagagttatgatcctcttgcaatgtaataaattgttagatggcattgtttttctttaccgtgaactggctagaattcttagtgatgttgtttacctaatagatgtttgaagtaacatgtgttctgaaacagtccgtcgatgctttcttggaggaattggttgtaaggagggaattagctgacaacttctgctattaccaacctcaatatgattcgctgtctggtgcatgggaatgggcaaggaagacactgatggatcatgctgctgataaaagagagcatatctatacgtgagtaagttgccctttataatttattgtcacaattttaaccaaattatcttcctgacgatgttaaggtaaaagatcttcgctctaattggttatttgttaattgtcattttgttttggctaggagggaacagcttgagaatgccaaaacacatgatcctgtaagtgctattggacgtggtgtactctgttgctgatattgaagttactctaacacactgagttattttttttctaaatgattatcagttgtggaatgcatcgcagttggagatggttcaccatggaaaaatgcatggattcatgaggtaatatatcatgaccagactcttacaaaatcacgcaattataaaatgtttattgaacagttttttttttatgaaactgcttgttatatttcttctgtacccatggcacattctagttagttgcccataacctattggcccacttgttgacaacatatggttattttacccatcatactgatggtgtggtgctaacctaaaggcattggattgcccccaccccatctttgctgacataactgattagtttgctttgttggatagatgggaattttctgacgttgtggtttactttatcctatgtcagaaaatatgaagttatttgaaaaaaagcattccttaaaaaattagttttctgtagagtttgtgtagttccaataaatttatgatgatctatgtgatatgtctggtagcaagcctttggtgcacataattctctttgattctgggcaaaccttttacactatttttgttcaacttttgttttggatctggaaagaaagaaaagtaagttgttttggcgaacatcaaagaatgatgttatatctaattgattcttagttctgaaggtaacagtgcctgtaaggactttttcctttcctaatgtagtagttgtctgtagaatgtactgggccaaaaagattctagaatggactagtggaccagaagaagcactttcaactgcaatttatttaaatgacaaggtaagacctatacacatgtttgggaaatacagttgtgttattttgaatttgtcctttttatagttttgctagttgtagttaaaatagctatatttcaacacatggaacatgataaagaaacatggaacatgataaagaaactgctcacatgaaaaattgtaattgaatagcaacttggaagagtctgcccatgcttaaattagttcctgcgccgataaccgaaaagttggagtcacatttatcaaattaagaaatatataatttgacattcttttcatgtaaggcagaaaattgtatggttggtgctcttttcattatcttatcttcccttaagttaaaaacatcatccttctagggacaaccagacaataaatcagcttcacggtcgcctcggataagctggactatcctttttcttggttacctgtcagaagaaagatacaaaacagtaactggggaaataaattttcaggaatgtgaaattgctcgaagatattttcatagttgaacctacaattctttccccagctggattgttctcgttctttaataggagtagttgtttgcattgaagtacttctacggattttatttatgcttttcaacaaagtcttacaaatcaatcttgtggcagtatgagatagatggcagggaccccagtggttacgtcggatgtatgtggtccatatgtggcctccatgatcaggtcattgtgttatcacttctcacttcacagggaaaaacaattttgcttttcacaagcattagattgttggtgacattctagaagcgagtattccttacaccactgctgttttctcagggttggaaggagcgtccagtatttggaaagatacgttacatgaattacgctggctgcaagagaaaattcgatgttgacgcttacatttcttatgtcaagagattagctggtcaatccaagaagaggaacgctgaggagtctccaaatcctgtagtcaagctttccaagtctcagcactaaatccgactaggaaaatatgaacatttctttgtgtggtataccacgagctcggaaaattgaagaacagtgctaacttgaggtagatcatgtagggtatacactgtagatctgtttgtctgatatccttgtaggcttctaatcattcaaaatttgctagcattgttaagactttaagacatacagtgtcacaccagttgaattcaagtgt</dnaseqindica> |
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