Os05g0543000

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Annotated Information

Function

“Pectins are fundamental polysaccharides in the plant primary cell wall. ”[1] “OSIPP3 is an anther-specific gene of rice encoding pectin methylesterase inhibitor family protein. Germination of pollen and pollen tube elongation are significant processes in plant sexual reproduction. The apical cell wall of the pollen tube is exclusively made up of pectin. Pectins are synthesized and methylesterified in the Golgi and are later released in the apoplastic space. Further these methylesterified pectin polymers are demethylesterified by a class of enzymes named as pectin methyl esterases (PMEs). PMEs are ubiquitous enzymes and play an important role in pollen tube growth. In plants, the activity of PME is regulated either by differential expression or by PME inhibitor proteins (PMEI) (Micheli 2001). PMEI inhibit the activity of PME of plant origin and have a role in regulating cell wall stability at the tip of pollen tube (Di Matteo et al. 2005).”[2]OSIPP3 gene (coding for pectin methylesterase inhibitor protein) was isolated from a pre-pollinated inflorescence-specific cDNA library by differential screening of stage-specific libraries from Oryzasativa. OSIPP3is present in the genome of rice as a single copy gene. OSIPP3 gene was expressed exclusively in the pre-pollinated spikelets of rice.

Expression

OSIPP3 is an anther-specific gene of rice encoding pectin methylesterase inhibitor family protein. 5‘-upstream regulatory region (URR; Khurana et al. 2012b) of OSIPP3 has been dissected by analyzing transgenic Arabidopsis plants harboring four deletions of upstream regulatory fragment (URF; will be used henceforth for denoting various deleted regions of OSIPP3 URR) for determining cis-regulatory elements involved in gene regulation. Four deletions of URR of OSIPP3 were transcriptionally fused to GUS reporter gene and transformed in Arabidopsis. OSIPP3_del1 and del2 transgenic plants showed blue color corresponding to GUS expression in root,anther and silique. Plants harboring OSIPP3_del3 construct showed GUS activity only in anthers and silique, while, pollen-specific expression was observed in case of OSIPP3_del4 transgenics.

Evolution

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Labs working on this gene

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References

[1]Maojun Wang, Daojun Yuan mail, Wenhui Gao, Yang Li, Jiafu Tan, Xianlong Zhang et al. A Comparative Genome Analysis of PME and PMEI Families Reveals the Evolution of Pectin Metabolism in Plant Cell Walls. PLOS ONE. AUG 12 2013. [2]Reema Khurana, Hitesh Kathuria, Arnab Mukhopadhyay, Sanjay Kapoor, Akhilesh K. Tyagi et al. A 286 bp upstream regulatory region of a rice anther-Specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis. Biotechnol Lett (2013) 35:455–462

Structured Information

Gene Name	Os05g0543000
Description	Plant invertase/pectin methylesterase inhibitor domain containing protein
Version	NM_001062735.1 GI:115465200 GeneID:4339485
Length	1347 bp
Definition	Oryza sativa Japonica Group Os05g0543000, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 5
Location	Chromosome 5:27009633..27010979
Sequence Coding Region	27009778..27009975,27010401..27010823
Expression	GEO Profiles:Os05g0543000
Genome Context	<gbrowseImage1> name=NC_008398:27009633..27010979 source=RiceChromosome05 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008398:27009633..27010979 source=RiceChromosome05 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggcgcgattcgtcatcatctccgtcgtcgtcgtcgccgccttcgccgctgccgctgtcgttgaggcccgggttggacctatcgacgtcgctccgaccaacctgatcaccaacccactcggtgccatcatcgacaatggccgcaagatcaccggcgccgtcgtcgacgagtgcgcctggacctgcgatcatgtcgcggcgggtaacaagaagatgtgcaacacgctgaggaagctgccgggggtgagctcgccgaaggagctcctgacggcggcggtgaagctgtcgatgaggaaggcgaaggcggcgagggcgaggttcgaggcggcggcgagggcggcggagaaggggacgccgatggagtccatcctggacacctgcaaggaagggtacgacagcacggtgtcggcgctgcaggaggtgcagcgctgcatcgacgccaacgacagcaaggcgagcctcatcaccaagatgtcggcggcgaccaccttcaccggcgactgcggcaacgcctacgaggagcgggagctggagcccagcctcgcgctcaaggccaccaagaacaacgtcaaccgcgtcgtcaccggcgccctcgccatcgccgccaagctcaagctataa</cdnaseq>
Protein Sequence	<aaseq>MARFVIISVVVVAAFAAAAVVEARVGPIDVAPTNLITNPLGAII DNGRKITGAVVDECAWTCDHVAAGNKKMCNTLRKLPGVSSPKELLTAAVKLSMRKAKA ARARFEAAARAAEKGTPMESILDTCKEGYDSTVSALQEVQRCIDANDSKASLITKMSA ATTFTGDCGNAYEERELEPSLALKATKNNVNRVVTGALAIAAKLKL</aaseq>
Gene Sequence	<dnaseqindica>146..343#769..1191#agccatcaccaaaccttttagcttctcagccttttgcctctctttctactctctttcttggccgtctctcttatatccctcaatttaaccatcgagggcggccaaaaaaaagagagaaaggaagagcaaaataacacattacaccatggcgcgattcgtcatcatctccgtcgtcgtcgtcgccgccttcgccgctgccgctgtcgttgaggcccgggttggacctatcgacgtcgctccgaccaacctgatcaccaacccactcggtgccatcatcgacaatggccgcaagatcaccggcgccgtcgtcgacgagtgcgcctggacctgcgatcatgtcgcggtgagtgacgacactattgtccatggaagcttattttgatcgctcgagagggtccctcgtatttatatgtcacttaaataattataaaaaaattaaaaaaattaagaagacgtgttaacctgtgatatatcaccccacaaacatacacgttcaaattcaacttctgtatctcgcaacgaaaaaaataaattaaaccaaatatagatgtttaatttgaacttggatatttgtgaagtgatatataacatgttaatacatcttctcgaattttttttaatttttttataactatttgagtgacatgtaaacaatgagggaatgatgttcctcgagggattaaaatccactcccgctccagaaatggtcagaaaattacttactgctcatgtcatggatgaacagtctgtctattgatgttgatgcaggcgggtaacaagaagatgtgcaacacgctgaggaagctgccgggggtgagctcgccgaaggagctcctgacggcggcggtgaagctgtcgatgaggaaggcgaaggcggcgagggcgaggttcgaggcggcggcgagggcggcggagaaggggacgccgatggagtccatcctggacacctgcaaggaagggtacgacagcacggtgtcggcgctgcaggaggtgcagcgctgcatcgacgccaacgacagcaaggcgagcctcatcaccaagatgtcggcggcgaccaccttcaccggcgactgcggcaacgcctacgaggagcgggagctggagcccagcctcgcgctcaaggccaccaagaacaacgtcaaccgcgtcgtcaccggcgccctcgccatcgccgccaagctcaagctataattagccaactgatcatcaatcgatatgaatgattgatcaactataaaattacacctagctcatgcatgcgtccgcgcgttatgtgtgtacgcacgcatgcatatgtgtaagatcgatcaacagatcacagaagtggtggcgtatctcgcacttgtg</dnaseqindica>
External Link(s)	NCBI Gene:Os05g0543000, RefSeq:Os05g0543000