Os05g0482400

The rice EUI1 (ELONGATED UPPERMOST INTERNODE) gene controls the growth of the first internode at the top of rice, and the recessive mutations of this site cause the abnormal elongation of internodes at the top of rice.

Annotated Information

Function

EUI gene is a recessive gene which was first reported by Rutger and Carnahan , which is characterized by the near doubling length of the uppermost internode, excess panicle exsertion and an increased panicle length in comparison with the wild type.The EUI mutant shows notably rapid elongation of the uppermost internode at the heading stage. Because of its prospective application to amend panicle enclosure in the male-sterile lines, this recessive trait, along with male sterility,maintainer and restorer, was referred to as the fourth genetic element of hybrid rice production .

The EUI1 gene was mapped on the central part of the long arm of chromosome 5, and located within two overlapping bacterial artificial chromosome (BAC) clones, OSJNBa0018K15 and OSJNBa0095J22, in an interval of 98 kb between markers M0387 and M01 .The EUI1 gene encodes a putative cytochrome P450 protein, CYP714D1. It can control the elongation of internodes and determines the plant height and underlies the grain yield .EUI1 plays a negative role in gibberellinmediated regulation of cell elongation in the uppermost internode of rice .

EUI catalyzed 16a,17-epoxidation of non-13-hydroxylated GAs. Consistent with the tall and dwarfed phenotypes of the eui mutant and Eui-overexpressing transgenic plants,respectively,16a,17-epoxidation reduced the biological activity of GA4 in rice,demonstrating that EUI functions as a GA deactivating enzyme.

Transgenic RNA interference of the Eui gene effectively increased plant height and improved heading performance. Furthermore, the eui mutant was defective in starch granule development in root caps and Eui overexpression enhanced starch granule generation and gravity responses, revealing a role for Eui in root starch granule development and gravity responses.

Rice internodes are important organs that affect plant height, yield, lodging resistance, and panicle exsertion,and also constitute a unique system for the study of gibberellic acid (GA) signaling. A spontaneous recessive tall mutant in rice called eui (for elongated uppermost internode), which shows notably rapid elongation of the uppermost internode at the heading stage, was picked up in a japonica variety of rice some years ago (Rutger and Carnahan 1981). The mutant (zeui stock) is characterized by a near-doubling in the length of the uppermost internode, extreme panicle exsertion, and an increase in panicle length, with little or no effect on other internodes or plant characters.This recessive genotype with the elongated uppermost internode is a very useful trait in hybrid rice seed production, and was referred to as the fourth genetic element of hybrid rice, in addition to the traits male sterility, maintainer and restorer (Rutger and Carnahan 1981). By means of a conventional breeding program, we successfully incorporated the eui locus into several kinds of male-sterile (MS) plants, including cytoplasmic MS and photoperiod/thermosensitive MS lines, to eliminate panicle enclosure, a phenotype which is typical of almost all MS lines (Shen et at.1987; Shen and He 1989; He and Shen 1991, 1994;Yang et al. 2002). Panicle enclosure in MS plants greatly reduces seed production by hybrid rice, necessitating the use of large amounts of exogenous GA to cause panicle exsertion. However, GA application not only increases the cost of seed production, but also greatly increases the rate of seed germination on the panicle, resulting in decreased quality and shortened storage life of hybrid seeds. In contrast, eui MS plants need little or no exogenous GA application for hybrid seed production (Shen and He 1989; He and Shen 1991, 1994). Therefore, the eui mutant represents a breakthrough in hybrid rice production (Zhang and Yang 2003).Previous genetic and physiological studies have shown that the eui phenotype is due to a recessive mutation in a single gene, which functions similarly in both japonica and indica subspecies and confers highest sensitivity to exogenously applied GA3 at the seedling and heading stages among the genotypes studied, although the level of GAs in eui plants is already higher than that in wild-type plants (He et al. 1993, 1994; Zhang and Yang 2003). These results strongly suggested that the Eui gene is likely to be involved in GA metabolism or signaling. Eui also seems to be quite mutable, because eui mutants have frequently been observed in populations subjected to chemical or c-ray mutagenesis (Maekawa et al. 1989; Yang et al. 2001; Zhang andYang 2003; Zhu et al. 2003). Molecular cloning of the Eui gene should therefore provide us with valuable information on its function in controlling internode elongation, as well as in GA metabolism and signaling.

Expression

Luo et al. found that EUI1 is expressed in most tested tissues and organs, including the young leaves, young roots, shoot apical meristem, the first and the second elongating internodes, flag leaf and young panicles. EUI1 is expressed at a relatively low level in the first elongating internodes, and the highest expression level was found in the young panicles during heading.

Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes, which could be partially reversed by supplementation with gibberellin.Furthermore, apart from the alteration of expression levels of the gibberellin biosynthesis genes, accumulation of SLR1 protein was found in the overexpressing transgenic plants,indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis.The rice EUI gene encodes a cytochrome P450 monooxygenase that deactivates bioactive gibberellins (GAs). A slight increase in EUI expression could dramatically change rice morphology,indicating the practical application of the EUI gene in rice molecular breeding for a high yield potential .

Evolution

The EUI1 gene encodes a putative cytochrome P450 protein. Cytochrome P450 proteins have been known as hemebinding enzymes with mono-oxygenase activities. Some members of the plant P450 family are known to be involved in biosynthesis of plant growth regulators such as gibberellins,jasmonic acid, auxin and brassinosteroids.The phylogenetic analysis of rice and Arabidopsis cytochrome P450 proteins shows that the EUI1/CYP714D1 protein belongs to the CYP714 subfamily of the CYP72 group. The CYP72 clan seems primarily to have conserved functions involved in plant hormone homeostasis. Three known proteins, CYP735A1, CYP735A2 and CYP72A1, belonging to the CYP72 group exhibit the greatest similarity to the EUI1 protein. CYP735A1 and CYP735A2 have been identified as cytokinin biosynthetic enzymes, and CYP72A1 is a secologanin synthase. According to the sequence similarity and the phylogenetic relationship between EUI1 and the three proteins, the EUI1 gene possibly encodes a putative cytochrome P450 involved in gibberellin homeostasis .

Labs working on this gene

• College of Life Sciences,Zhejiang University, 310029 Hangzhou, China
• SHARF and National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, China
• College of Life Science, Chinese Northeast Agricultural University, 150030 Harbin, China
• Chinese National Rice Research Institute,310006 Hangzhou, China
• College of Life Science,South China Agricultural University,510642 Guangzhou, China
• National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China
• China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, PR China
• National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences,Shanghai 200032, PR China
• College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China
• The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, PR China
• Institute of Genetics and Crop Breeding,Fujian Agriculture and Forestry University, 350002, Fuzhou, China
• Hainan Institute of Tropical Agricultural Resources, 572025, Sanya, China
• RIKEN Plant Science Center, Kanagawa 230-0045, Japan
• Biotechnology Institute, Zhejiang University, Hangzhou 310029, China
• Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
• Zhejiang Academy of Agricultural Sciences,Hangzhou 310021, China
• Institute of Insect Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China

Structured Information

Gene Name	Os05g0482400
Description	Cytochrome P450 family protein
Version	NM_001062401.1 GI:115464532 GeneID:4339131
Length	9805 bp
Definition	Oryza sativa Japonica Group Os05g0482400, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 5
Location	Chromosome 5:23775727..23785531
Sequence Coding Region	23775837..23776191,23784066..23785444
Expression	GEO Profiles:Os05g0482400
Genome Context	<gbrowseImage1> name=NC_008398:23775727..23785531 source=RiceChromosome05 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008398:23775727..23785531 source=RiceChromosome05 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggagagcttcttcgtcttcttcacggcggcggcgttgccggtggtggtggcggcggcggtgatcgccgggctgtgcattacggcggcgtggctggcgaggccgcggcgcgtggcggaggtgttccggaggcaggggatcgacggcccgccgccgtcgtcgttcctggcggggaacctcccggagatgaaggcgagggtggccgccgcggcgtcggcggcggcgccaacggcggacggggaggagaccgcctccgccggcggcggcggcggtggccgggacttcgagaaggacgggttcgacgactactgcaccaggatcttcccttacttccacaagtggaggaaagcctacggcgagacgtacctgtactggctgcggcggcggccggcgctgtacgtgacggacccggagctcatcggcgagatcgggcggtgcgtgtcgctcgacatgggcaagcccaagtacctccagaaaggccaggagccactcttcggcggcggcgtcctcaaggccaacggcgcgtgctgggcgcgccagcgcaaggtcatcgcgccggagttctacatggcccgtgtcagggccatggtccagctcatggtcgacgccgcgcagccgctgatcgcctcctgggaatccaggatcgacgccgctggaggcgcggcggcggcggaggtcgtcgtcgacggcgacctccggagcttctccttcgatgtgatatcgcgggcttgctttgggagtgattactcgagggggagggagatcttcctccgcctccgtgagctgtccgggctcatgtcggagaccagcgtcatcttcagcatcccttcgctgaggcacctgccgacggggaagaaccggaggatctggaggctcacgggggagatccggtcgctgatcatggagctcgtcagggagcggaggtgcgcggcgagggcggcgagggagcacggcgggaaggcggcgccgccgtcgccgccggagcgcgacttcctcggctccatcatcgagaacagcggcgggcagccgcggccggacgacttcgtggtggacaactgcaagaacatctacttcgccgggcacgagacgagcgcggtcaccgcgacgtggtgcctcatgctgctcgccgcgcacccggagtggcaggaccgcgcccgcgccgaggtgctcgaggtctgcggcggcgacggcgccgccgcccccgccgcgccggacttcgacatggtgtcccggatgcggacggtggggatggtggtgcaggaaacgctgcggctgttcccgccgtcgtcgttcgtggtgcgggagacgttccgggacatgcagttgggtaggctgctggcgcccaagggcacctacctgttcgtcccggtgtccaccatgcaccacgacgtcgccgcctggggcccgacggcgaggctgttcgacccgtcccgcttccgcgacggcgtggcggcggcgtgcaagcacccgcaggcgtcgttcatgccgttcggcctcggcgcccgcacctgcctcggccagaacctcgcgctcgtcgaggtcaagacgctcgtcgccgtcgtcctcgcccggttcgagttcacgctctcgccggagtacaggcactcgccggcgttccggctcatcatcgagccggagttcggcctccgcctccgcatccgccgcgccggcggtcaggacgccacgtcacaagttgacacatctactgcacccgtgcatagttctcataattaa</cdnaseq>
Protein Sequence	<aaseq>MESFFVFFTAAALPVVVAAAVIAGLCITAAWLARPRRVAEVFRR QGIDGPPPSSFLAGNLPEMKARVAAAASAAAPTADGEETASAGGGGGGRDFEKDGFDD YCTRIFPYFHKWRKAYGETYLYWLRRRPALYVTDPELIGEIGRCVSLDMGKPKYLQKG QEPLFGGGVLKANGACWARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAA GGAAAAEVVVDGDLRSFSFDVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIP SLRHLPTGKNRRIWRLTGEIRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGS IIENSGGQPRPDDFVVDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEV CGGDGAAAPAAPDFDMVSRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKG TYLFVPVSTMHHDVAAWGPTARLFDPSRFRDGVAAACKHPQASFMPFGLGARTCLGQN LALVEVKTLVAVVLARFEFTLSPEYRHSPAFRLIIEPEFGLRLRIRRAGGQDATSQVD TSTAPVHSSHN</aaseq>
Gene Sequence	<dnaseqindica>111..465#8340..9718#aagggcgaggtgagcgaggcgagactcgagagagaggagaagatccagaaatcgcaagctgcacgctatagctaggtagctatctccggggaaagagagagtaggctgccatggagagcttcttcgtcttcttcacggcggcggcgttgccggtggtggtggcggcggcggtgatcgccgggctgtgcattacggcggcgtggctggcgaggccgcggcgcgtggcggaggtgttccggaggcaggggatcgacggcccgccgccgtcgtcgttcctggcggggaacctcccggagatgaaggcgagggtggccgccgcggcgtcggcggcggcgccaacggcggacggggaggagaccgcctccgccggcggcggcggcggtggccgggacttcgagaaggacgggttcgacgactactgcaccaggatcttcccttacttccacaagtggaggaaagcctacggtacgtgtacacaagcctctctcctctctactgctcgatttcacatcactagctacatgcatgatgcttgcttgctttgcctgttaattactccgctcatgtgtgcttaattagcttaattaattcacactccggttaatcaagtcatgcaaaacccacaattaatcagctatcaattgagatgagtatataccagaatatatatacttacagtaaatttaataaaaaaataaaaaaaagaaaagaaaagctctctcgatctctctgtgacgatgatacaagaaatttgtggttgccctagtaacaagttgactagagtataaaagctgctaaaatgtcaagagctctccatataaaaaagaacagtgggggagaataattaatactgtcccaaacagcgagaggaagagagggggcaaacgagaaaagagagaataaacaaaattaatgcaggatggaaaccgtggttgggacgaactactcggtttgcttccagtcaaccgtcctgcatcattaagctatggattaataattaattaactgcagtatataaatatatatactcccccacatatattaatcggtcgttggtccaattagacaacagcaaagccatgcctaggtacctgtctaggtaggctatgactgtagtacactcgtactactcacttaatagcacattagtgctcttattttgcgaattaatgaaactttaatcaataataactatatataactataatatatttagtataatttaacaatatatctattcagcgtgctatcgtttcttcagcctatcaatcaacttaaaaaacttagtgttaaattgattttggtgttttcttatcgtaatttttcttcagttttggattgttttttaagttgctaacaattttagttaacaaagctatttggaaggagaggaataatgcatccatgacaaaagtatatgcttcataataattttcatataccagatcttgcccctggtggtgacgtgttaccaaataccgactggaattaatttcttctctctttctttttctctctgccggtgcaatgaggcgacatttttgagttataactgtgcgtatgaatgcatgtattaattgctgtttgctgaggttaataggctagcaataccgttgttttgggggaccccgcagtttgacctagtagagtttatcatggacgcagactgcccctacctgttcttcttcacggacagcatgattaattagctatactagtggtagtactcgatgtcattaataattggtctctccgtacgtgaataattatcgatttttgactttgacaagtactccctccgtacttgttaaggaagtcgttttagacagcaacacggtctccaaaacacaactttaacttcttatttctataaaaatatttattgaaaagtgatatatgtatacttttatgaaatattttttaagacaaatatattcatataatttttacattttaaaacttaatggaaagttacttttccaagatttgactcaaacattgttctaaacgatttcctttatgagtacggaaaaagtatgttttttcactgttcttatgaagtgtacatggaaagtgatcgaatagctctgagcagtactctaggtagggaattacttttagttgcgtacaaatctaacaattaattaatatgccatttttagtgaacataattgtatattttctccgtttcacaatgtaagacattctagcattttccacatgcatattaatgttaatatgtctagattcattaacatcaatataaatataagaaatgctagaatgacttacattataaaacggaggaagtaatttatgctagaatgacttacattataaaacggaggaagtaatttagtacatcaccttcgtcgattattacgaaaacatccatcatatcgatcgatatctgtgtatttgctactccagaaaaatattttctgagaccgacccacagatgcatatatatgcacattattgtggcggttttcctttctttttagatcaatcggtggcctgacggccggcccagcttcgattgaaattaagctgaataattattgtggccgtcatgtgctaatcacccatataattaggcgtggttagaacctgactaagctatttaagccggtcagttaggctttcccaaaacggccatcaggccatgcatgttgccattagctgtccgagcacgccggccaaagctcaaccctcgtgatcacatcaactcgatctccccgatgcttatccctttttgcaagcaaccggtatgtggtgcttagataattaatgcaagaattaacagtgtacctcctatcaatttgccgttagctatgcatgtaatggattagaaattctatatgcatgttttcgtctaacagagtttgatcagcagcagtacttagctaactaattattctgtaatttatataactactataagctagagtttaagcagagtatgtaagtatgatattttaactaatttagttcttgtcatatggcactgtagttgaaaccataaaccgatcaattaataggacaaatattcaacctggttgcctcactgcatgcgttgttttagtttgacaaatgcatgcatgtgtatgatattttatttgcttggcccttaataagtatattatgttgaagtcatgcatcttgacacagaaacttggagcaaaaactatataatatatttcaagaaccaaagggcctgaatactagtacttgcctacatcgatcacatgatgctagtgtcaatcttatttaaggtttttatttcttctttaatgtcaaaatggctattgtttaactatctaaaaacaccaataattagaagactcccactagaccatctcatgatgcattgcacgtaggatcgatatatatggcaacattaattattttacttgaacatctaatcgcgttcgctattattcaacttatgttacatgttctcccctaatttaagcaaaggccggtttgaccaacgggaaacaaataattaaattgatcttccaggcccagtacttatattaattacctagtcattaactacattttctccatatttaatttgaatgcttcgttctgaaatatttaaaggccattaacactaacacggcacacaagaaattaatttaaatatttaatttggctagcatgtatacatatatatgttgatatgcatggatatatatatatatatatatatatatatatatatatatatgtatatatatatatatgtatatatatgtatatatgtatgtatatgtatatgtatatgtatatgtgtgtgtgtgtgtgtgtgtgtgacaatagctaactttagatatgtttttttttcgatgatacatggtcccggcctctgcatcacaaatgcacacatccaacgaatatcgtacaaataataataaaaaaagtgatgggacaaaactcccgactcctacataagacttaggactagtgagctaagggtgtgtttagttcaccaaaattggaaatttgattgaaattagaatgatgtgacggaaaagttgaaagtttgtgtgtgtaggaaagttttgatgtgatggaaaatttggaagtttgaagaaaaagtttagaactaaactcggcctaactgaaaacaaatcaattgatatttgttttcagttatgccgagtttagtttgacaaaattaaacgaaaccatacaatatataattacaaatcaataattaattcctaatgctgcttgatgctactagcttctctcctacacagtgccattgactaggtaattgctcctaaccctagctagctaagcatgcagctcgatcgaatcgatcgagcatgaaagcaacaggtcgatcgatagatggtaagccatgacgatcaatatgatcatatcatgcatgcatgacactgtagagtgtgtactacgtactaccaccactccaccaaccatgcatgcattcctcccccctctctcttgcaccttttaaagctagctaggacgtgtggggacttcaatatatttcttgtgcattgtctctcctacaaggattccacatggagatgtgcatatgcatggggttacaccaattaaaagtgcagcccacactctatggattgaaggtgctagctagatctctgtactctatccgtcccaaaatataagcatttttagttataaatccagatagatagttatccagattcgtagctaaaagttattatattttaggatggaggtaatatagtttagatggtggaaattaagtctagtagctatggggaaaaatggcaaaaataaaattacagtatggggttgtactggaaaattttcatttgaatatatctcttcacaaatatgtaagcctaaatttaatctaaatatatatgggcacaaatgaattaaaaaacatattttctatgaacatatatagcaggaataatatgcattcctcgattttattcccttcaagtgttcatatagtacgttaaaagtttgggttaaaaattttaaataatttctttagttattttgaaatggtatgaatgaaataagagcgtatacccttaagtcataaaagatcatccagattgaataaaaaaacaaagttaagataattaagtagatgaatgtaaatgtgcatatacatatcttgtctttctgttgactagttgtctgttcagaaattatatggcggaaaccctaactgaggtgaaaattcatgtgtaccaagacaaaaaaagaaagttctgaaatcacacaaacaatctcataagtagataagatgatagtgaacaaccatgtaaagatcttttccaaatttaactttcatttgtcagatataaaatgacaaattttgaatcagaaaatattttttttgtttgaaatttaccctcgtttcttgatctgaacagtaccactagatacacctagctacatgtatggtcgattgtgataggctcacacacatgctatagcttgatggttgtcatggtggttgcctagttggcaaaagttcgatgtccagctgtgctactatcatgataattggcagcagcctagctagctagttcaaggggacaatattacactggctttaataatttcctcgtcaccaccttttacctcaaaaacatattcttgaaaaaatgaagaatatgatcatttcaccaaaaatcatggactggccatgattaaccaactcattttggatggttttgttggattcctcaaaccagacacatcattctctgtgtcgagaatggagatgacgtgactagcttttatgactttagaaacatactccctccgtctcaaaaaataaactttaggtttctgtgtccaatgtttgatcgtatatcttatttgaaaaaaatatgaaaaaaattaaaaagacaaatcacgcataaagtattaatcatattttatcatctaacaataattcttttcgtgcttgtgattgtgagtattggaattcttttaatatatgctgaatatactaatgagcataaagtataacagactaatcaagtatgtgagttattcaatccttggcaatttccaatacaaatacagacacactatgtatggaacttttttatatatagtctattaaacaatatatctttgagccgtctgaaaccaaattatatgatatttttttagttaatcataccaagatcattaattgagtaggaatataagacttcaagggggaatcaacaatattaattgtttctgagaacaattattcaaagcaattcataataaaagcatatttattttaggagtgggtacacacactatctagcttgtaccatatataagtcaatgaccacgatatatggctagccacctccctccatcatgaaataaattaacctcatacgaatttagatatagaatatgtccagatttattgattaaatcctagattgatttattttaggacgaagagattacctgcctgcctgcactaaccatctgcgacgccgacgtgtcatccatcggtcgacaaaacgtacgcaggccaacacatgcatacgtacacacaattgcccatttgcaccccgctccccatgtccacgtcagacggcgacacgtacacattaacacatgtacacgtcgttttcacgtacgagctaagcaaatccaacgtacagaaaatagttaatttacccggttgccaactgggacacgtacgccggccacgtcacaggtctacgtggcgcggtgtgggccgggctaggagggttaaaagcccacgcttgtgtgctttggtcaactcgtgcacttattactctgaagagttattagccggttcgatgtgtcgcgattactgttgtaattaagtctgtctctgtggcgatatgtacgttgacgatgattaaggtggtgtttggatctagagacttaactttagtctctgtatttagacactaatttaaagtattaaatatagactaattacaaaactaattacataaataaaaacttatttgcgagataattttttttaagcctaattaatccataattagaaaatatttaatgtagcatcacataggataatcatgggttaattaggctcaatagaatcgtctcgcgaattagtctaagattatggatagtttttattaatagtctacgtttaatatatataattagtgtccaaacatccgataagatagagacttaaaagttttaggtggtgtttagatctagggacttaactttagtccctatatttagacactaatttagagtattaaatatagactacttacaaaactaattacataaataaaagctaattcgcgagataatttttttaagcctaattaatatataattagagaatgtttactgtagcatcacatatgctaatcatggattaattaggctcaatagattcgtctcgcgaattagttcaagattatggatgggttttattaataatctacgtttaatatttataattagtgtccaaacatccgatatgatagggacttaaaagttttaatctcatctaaatatggttttagtctcatctaaacagggtctaatagcagaaagttacgtgagattcgtatactcgtacgtacgtatttgcatcggaattttcaaaatcctagactctttctagtactggtggtacagtttggcaaatctggttttttggatggtttcagcatgtttaatttgcagtgtggaattgttgagatgctagagattttttgcagaacttatgtgaaatttaattaggcgtttcaactgtggctaaacgtgaagatatagcgcgctagagacgtgtgtgttggcagtgttgctttgcaatttgcataggctcgtggtgtttggttcagaaatttatttaaggatccggttgttttttttttcattttccttggtcaatgttgtaaagaatccgtagaatcttggcaagaagattctcccaaacctccaggaaagaggagaagattctccggaatcagtggaagtagtgcagtagctattgactgggatttttgtaagcccggtcaataggttcccctgtacccttggagagaccctgcaagtgcacacagatcacgtatatatccacatgcctatatgtgcatagatcacataggcacgcatcaaaagctttttaatcctagctagcttactttaatctttgattattttcaatagaaatttaatgacgtttaacttgattcatcattcggcgacgtctcttgaacatgttaaaggctgggccgaggcccaccaaaaacggaggcccatgagcaaatttcaaattctgactcgcgcgcggtggcggcaaaattttgcaggcgagacgtacctgtactggctgcggcggcggccggcgctgtacgtgacggacccggagctcatcggcgagatcgggcggtgcgtgtcgctcgacatgggcaagcccaagtacctccagaaaggccaggagccactcttcggcggcggcgtcctcaaggccaacggcgcgtgctgggcgcgccagcgcaaggtcatcgcgccggagttctacatggcccgtgtcagggccatggtccagctcatggtcgacgccgcgcagccgctgatcgcctcctgggaatccaggatcgacgccgctggaggcgcggcggcggcggaggtcgtcgtcgacggcgacctccggagcttctccttcgatgtgatatcgcgggcttgctttgggagtgattactcgagggggagggagatcttcctccgcctccgtgagctgtccgggctcatgtcggagaccagcgtcatcttcagcatcccttcgctgaggcacctgccgacggggaagaaccggaggatctggaggctcacgggggagatccggtcgctgatcatggagctcgtcagggagcggaggtgcgcggcgagggcggcgagggagcacggcgggaaggcggcgccgccgtcgccgccggagcgcgacttcctcggctccatcatcgagaacagcggcgggcagccgcggccggacgacttcgtggtggacaactgcaagaacatctacttcgccgggcacgagacgagcgcggtcaccgcgacgtggtgcctcatgctgctcgccgcgcacccggagtggcaggaccgcgcccgcgccgaggtgctcgaggtctgcggcggcgacggcgccgccgcccccgccgcgccggacttcgacatggtgtcccggatgcggacggtggggatggtggtgcaggaaacgctgcggctgttcccgccgtcgtcgttcgtggtgcgggagacgttccgggacatgcagttgggtaggctgctggcgcccaagggcacctacctgttcgtcccggtgtccaccatgcaccacgacgtcgccgcctggggcccgacggcgaggctgttcgacccgtcccgcttccgcgacggcgtggcggcggcgtgcaagcacccgcaggcgtcgttcatgccgttcggcctcggcgcccgcacctgcctcggccagaacctcgcgctcgtcgaggtcaagacgctcgtcgccgtcgtcctcgcccggttcgagttcacgctctcgccggagtacaggcactcgccggcgttccggctcatcatcgagccggagttcggcctccgcctccgcatccgccgcgccggcggtcaggacgccacgtcacaagttgacacatctactgcacccgtgcatagttctcataattaattggcttctgtaaacagctagtaagtcgtgaactgaaaaaaatacatcacacatacagagacaacagaggattaccattttaccagc</dnaseqindica>
External Link(s)	NCBI Gene:Os05g0482400, RefSeq:Os05g0482400