File:Rice2.png

Genetic andphysical maps of the gw-5gene on rice chromosome5. (A) QTL mapping ofthe qGW-5 locus. (B) SixBAC/PAC contigs aroundthe gw-5 locus. (C) Finemapping of gw-5 usingnewly developed SSRmarkers. The red super-script a indicates that thereare 167 recombinants ofthe total 805 homozygousplants between the gw-5gene and the markerRM5874. In fact, the 167 re-combinants included 113wide-grain plants with theheterozygous band patternsof wide- and narrow-grainparents and 27 wide-grainplants with the band pat-tern of the narrow-grainparent. The recombinationfrequency ? (N11 N2/2)/N, where N is the total num-ber of wide-grain plants sur-veyed, N1is the number ofwide-grain plants with the band pattern of the narrow-grain parent, and N2is the number of wide-grain plants with the hetero-zygous band patterns. We marked the number of recombinants between RM5874 and the gw-5 gene as 167 to directly calculate therecombination frequency, [(27 3 2 1 113 ? 167)/(805 3 2)] 3 100 (cM). The other numbers marked in the row (no. of re-combinants) were also calculated on the basis of this approach.