Os01g0571300

OsHsfA7 is a number of heat shock protein (HSP) genes in rice, which play an important role in plant stress tolerance and mainly regulated by heat shock transcription factors (Hsfs) .

Annotated Information

Function

• HsfAs not only responded to high temperature but also to oxidative stress, high salinity, chilling and other stresses.
• OsHsfA7 has an important role in root growth and development. OsHsfA7 may play a role as a member of natural defense system against high salinity and drought stresses.
• OSHsfA5 and OSHsfA7 transcripts were at correspondingly low levels in all tissues; while transcript levels of OSHsfA5 were lower in roots, those of OSHsfA7 were slightly higher in leaves.

GO assignment(s): GO:0004672, GO:0004674, GO:0006468, GO:0005524

Mutation

Figure 1. Root morphology of wild-type and OsHsfA7-OE transgenic plants.(from reference ).

Root morphology of the WT and OsHsfA7-OE transgenic plant seedlings was shown in Figure 1. OsHsfA7-OE plants exhibited longer young roots (including primary root and adventitious root) (Fig. 1A) but shorter and less lateral roots (Fig. 1B) and root hair (Fig. 1C) compared with the WT. Average radicle length of 5 d OsHsfA7-OE seedling was 3.7cm, and that of WT was only 1.5 cm, the results showed that the young roots of transgenic seedlings grew faster than the control. Moreover, the roots of OsHsfA7-OE at tillering stage were thicker, sparser, more wide distributed and almost no lateral roots compared with that of wild type.

Expression

Figure 2.Influence of high salt stress on rice seedlings.(from reference ).

Figure 3.Phenotype of the OsHsfA7 over-expression transgenic plants in response to drought treatment.(from reference ).

1 over-expression of OsHsfA7 can improve salt resistance of transgenic rice:

• After irrigating with 200 mM NaCl for 10 d, leaf apex of WT plants became brown and dried, whereas over-expressing OsHsfA7 leaves remained green (Fig. 2B).
• Leaf REC and MDA content were lower in the transgenic lines than in the WT (Fig. 2E, F).
• Hydroponic seedlings were treated with 200 mM NaCl for 24 h then transferred to 1/2 MS solution for recovery. After 4 d, leaves of WT were completely curled and wilted, while those of OsHsfA7-OE were rolled only in the tip part (Fig. 2C).
• After 10 d recovery, leaves of WT were withered and almost all plants were completely dead, while most of the transgenic plants remained alive and only the leaf tips scorched(Fig. 2D).

2 thermo-tolerance and drought stress：

• To examine the tolerance to drought stress, three-week-old plants were withheld water for 10 d and then re-watered for additional 10 d. Both WT and transgenic plants suffered severe blast after un-watering (Fig. 3B).
• Whereas there was remarkable difference after re-watering. While most of the WT plant leaves showed further withered and could not be rescued, the majority of OsHsfA7-OE plants restored normal growth (Fig. 3C).

3 Meanwhile, we tested the thermo-tolerance of transgenic and WT plants at booting stage, no significant difference was found in seed setting rate after treatment.

1. However, the transcript levels of OSHsfA4b, OSHsfA5, OSHsfA7 and five OSHsfA2s were significantly up-regulated during early stages (OSHsfA2a, OSHsfA2c, OSHsfA2d, OSHsfA4b at 15 min, OSHsfA2d, OSHsfA2e, OSHsfA7 at 45 min and OSHsfA5 at 1.5 h) of heat stress exposure, with the effect gradually diminishing when prolonged heat stress.

4 short-term heat stress and calcium on HSF expression:

• OsHsfA7 has little or no expression when calcium and heat stress are given separately but showed some expression when both these treatments are combined.

5 PEG stress:

• OSHsfA2a, OSHsfA2e, OSHsfA3, OSHsfA4d, OSHsfA5 together with OSHsfA7 genes responded more drastically by PEG

6 Among the three Hsfs, OsHsfA7 was significantly upregulated in shoot (11.8-fold) and root(13.3-fold) after SDS.
7 Expression of AtHsfA7a was reported to be elevated in cells during light stress. OsHsfA7 responded to all the four treatments(Heat,Salt,PEG,Cold).
8 OsHsfA2a, OsHsfA2c, OsHsfA2d, OsHsfA7, OsHsfA2f, OsHsfB1, OsHsfB2a and OsHsfB4c, genes showed high TA values during HS and OS treatments in Q-PCR.
9 Dheeraj Mittal et al. propose that OsHsfA2f and OsHsfA7 may be involved in the late response to the oxidative stress.

Evolution

Please input evolution information here.

Labs working on this gene

• Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University
• College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China
• Department of Plant Molecular Biology, University of Delhi, South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi 110 021, India
• Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Japan
• Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Furong District, Room 420, Life Science Building, Changsha (410128)
• Institute of Rice Science, Hunan Agricultural University, Changsha, 410128
• Directorate of Rice Research, Hyderabad, Andhra Pradesh, India
• Centre for Biotechnology, Institute of Science & Technology, Jawaharlal Nehru Technological University, Hyderabad, India
• Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha City, Hunan Province, China

Structured Information

Gene Name	Os01g0571300
Description	Similar to Heat shock transcription factor 31 (Fragment)
Version	NM_001049913.1 GI:115437775 GeneID:4326351
Length	2933 bp
Definition	Oryza sativa Japonica Group Os01g0571300, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 1
Location	Chromosome 1:23595710..23598642
Sequence Coding Region	23595711..23596172,23597579..23598325
Expression	GEO Profiles:Os01g0571300
Genome Context	<gbrowseImage1> name=NC_008394:23595710..23598642 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008394:23595710..23598642 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atgcttaaaccccaaacacctcgcgctcgccgcgccgcgcatcccaattcccacatggcgtcctcctcctcctcctcctccctctgccgactactcatccccaggccgaccacccgccgcttctccggaggaggaggggaggggggaatggcggcggcggcgccggtgaagcgggaggtgaagcccgaggcgggggaggggtggggcgggggtgatctgggggtggtgccgccgccgccgcggccgatggaggggctcggggaggcggggccggcgccgttcgtggcgaagacgtacgagatggtggcggacgcggccaccgacgcggtggtgtcgtgggggcccggcggctcgggggcctccttcgtggtgtgggacccgcacgcgctcgccgccggcgtgctcccgcgcttcttcaagcacgccaacttctcctccttcgttcgccagctcaacacctacgggttccgaaaggtcactccagatcggtgggagtttgcaaatgaagccttcttagctggtcagaagcatttgctgaaaaacatcaagaggaggcgtgtttccaagcctctcgtggattcacaactaagaaataaagcaagtgttgtttttggacagcctgaggctcctggtgaggttgtgagcttgaagagagaccgtgcagccctcagagctgaggtcatcatgcttaagcagcagtacaacgcctgtaaatctcagctcattgccatggaggagatggtccgcaacatcgagagaaggcaacagcagaccatcggcttctttgccaaggtactcaccaacccggctttcgtgcagcaggttctactcaactatgtgaataagaatggcctaagaggcgccgcgaaaagacaacggctgatggaaaacgaagaacagcatgctgattcgccattgaacaaaggtatggaagctgcatctgtgatggaagctgatgtttctccagggagcactggctgtggcacagttgggaaagtggagaccactccaatgtgcaatttccagaatattgagaatatgtgtgatgatgtctgggaagagctggacgccttacctgaaactggaatggagcaggaagaaaaggctgggattggctccttcgatgtcgaggagttcgtcggaaggccttgtggttgggttgatgactgcccataccttgtggagccgatgcagttcgtggaacactag</cdnaseq>
Protein Sequence	<aaseq>MLKPQTPRARRAAHPNSHMASSSSSSSLCRLLIPRPTTRRFSGG GGEGGMAAAAPVKREVKPEAGEGWGGGDLGVVPPPPRPMEGLGEAGPAPFVAKTYEMV ADAATDAVVSWGPGGSGASFVVWDPHALAAGVLPRFFKHANFSSFVRQLNTYGFRKVT PDRWEFANEAFLAGQKHLLKNIKRRRVSKPLVDSQLRNKASVVFGQPEAPGEVVSLKR DRAALRAEVIMLKQQYNACKSQLIAMEEMVRNIERRQQQTIGFFAKVLTNPAFVQQVL LNYVNKNGLRGAAKRQRLMENEEQHADSPLNKGMEAASVMEADVSPGSTGCGTVGKVE TTPMCNFQNIENMCDDVWEELDALPETGMEQEEKAGIGSFDVEEFVGRPCGWVDDCPY LVEPMQFVEH</aaseq>
Gene Sequence	<dnaseqindica>2..463#1870..2616#aatgcttaaaccccaaacacctcgcgctcgccgcgccgcgcatcccaattcccacatggcgtcctcctcctcctcctcctccctctgccgactactcatccccaggccgaccacccgccgcttctccggaggaggaggggaggggggaatggcggcggcggcgccggtgaagcgggaggtgaagcccgaggcgggggaggggtggggcgggggtgatctgggggtggtgccgccgccgccgcggccgatggaggggctcggggaggcggggccggcgccgttcgtggcgaagacgtacgagatggtggcggacgcggccaccgacgcggtggtgtcgtgggggcccggcggctcgggggcctccttcgtggtgtgggacccgcacgcgctcgccgccggcgtgctcccgcgcttcttcaagcacgccaacttctcctccttcgttcgccagctcaacacctacgtacgtatcccaatcgcccgatctgctgcttgtggtgtgggtggtgttctttctgtgaaccgctgatcggtctcgtgtgcttctgtttcccgtgtaaattgaatcgttgatgctggtgagacaatagttgttagttgatcgaacaagataactgattatgagaatgaaatttgaaaaggagtgataccaaattcgtgggaaatccttgctgcgtttccgtactaatcaagaatcgtatcatgtatgaactggtactgtcctatgctgtggtttgttcgtgtccacgaatgcgctcgcatatacaatatcctgttttgtcaaaattggtgataatagcaggagaattctagaagtttgaccagaatttaaaaatcatgcctaaattggttaatattggactgcaaatggtatagaaccatcccttgtatatacaattttgttccacctctagtttaggatggtgcataccagaaattttgtcttaccaagaaactgaaatcctcagccatgtttttgtaggatgctgaatgttcagtggtggtatacttgtattgtacgatgcaaagtcatgctagttacttgcatccatctgtactacgggacaaatgtttgtcttggtttttagcccctttgtgatgatgcacttggattggatttcatctgctccactgtcactggatatgacctatgttctgtctgttcacgttgcaatccaagatactgatccctaaaatacttaaagtggataatattccttcttgtgggcattacaagtgtccaggtagctcgtatgtcgtactcgaaatcttgaagtctttttccaccactctgaaagacctaatacctcatgacctgtccatacctttaatagttaaaagggaaaggtgatggtaacatgatctaggtgaactatgctcccccactttttcttttgtcttttttgatgatgtggagctccgtgcttttgtacatacatgagcttatctgattcaatgagctacagaagtacagaacagttaccttgttcttgtcaatgagcttatctagcagtgtgttctcggttatttggcctgtttgtgtttattgctaatggcaccaccattcttgctgttttgatattatctatatacttttcttgctgtaaatagacatttcatatcaggcaactgaaccttctagcttctgggtaatgctggtcctgtggttcaacggtagaacacagaagtgaaattgctagtttaatctctgatagactaaaaccatagactgattcttttgttaatatgcgtatacctaccattgacagttttcaatccctaaatactgctgactttctagactgggtttggttccctgcagctgcttctagaaaaacttaccggcctctacctgttgttgctatgcaggggttccgaaaggtcactccagatcggtgggagtttgcaaatgaagccttcttagctggtcagaagcatttgctgaaaaacatcaagaggaggcgtgtttccaagcctctcgtggattcacaactaagaaataaagcaagtgttgtttttggacagcctgaggctcctggtgaggttgtgagcttgaagagagaccgtgcagccctcagagctgaggtcatcatgcttaagcagcagtacaacgcctgtaaatctcagctcattgccatggaggagatggtccgcaacatcgagagaaggcaacagcagaccatcggcttctttgccaaggtactcaccaacccggctttcgtgcagcaggttctactcaactatgtgaataagaatggcctaagaggcgccgcgaaaagacaacggctgatggaaaacgaagaacagcatgctgattcgccattgaacaaaggtatggaagctgcatctgtgatggaagctgatgtttctccagggagcactggctgtggcacagttgggaaagtggagaccactccaatgtgcaatttccagaatattgagaatatgtgtgatgatgtctgggaagagctggacgccttacctgaaactggaatggagcaggaagaaaaggctgggattggctccttcgatgtcgaggagttcgtcggaaggccttgtggttgggttgatgactgcccataccttgtggagccgatgcagttcgtggaacactagaggcatgagaagaatacaatctgtccttagaatagtggaacgtgtttataacgtctgaatatctgtatatcaattacccacttcgatagaagtctgtactatgaatagcataagctcctggaacagagcttaaatataggaaacgaacactctcaggtaatgcaagccaggagtttgtaggattgaagatttgttactgtaaattctgctgggcgcagcaaaaaaatcgtgatgttagatatacaatactgtctctgcaatgtagccgaggaaaaatacgaatgccagtcttatcaatgtgaatactttatactgtt</dnaseqindica>
External Link(s)	NCBI Gene:Os01g0571300, RefSeq:Os01g0571300