Os05g0552300
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The gene Os05g0552300 encodes a protein called the receptor for activated C kinase 1 (RACK1), which is one member of the most important WD repeat–containing family of proteins found in all eukaryotes and is involved in multiple signaling pathways[1].
Contents
Annotated Information
Function
The gene is composed of 2 exons and encodes a protein of 336 amino acids, RACK1(Receptor for Activated C-Kinase 1).RACK1 functions in various mammalian signaling pathways and is involved in hormone signaling and development in plants. Rice contains two RACK1 genes, RACK1A and RACK1B, and the RACK1A protein interacts with the GTP form of Rac1. Rac1 positively regulates RACK1A at both the transcriptional and posttranscriptional levels. RACK1A transcription was also induced by a fungal elicitor and by abscisic acid, jasmonate, and auxin. Analysis of transgenic rice plants and cell cultures indicates that RACK1A plays a role in the production of reactive oxygen species (ROS) and in resistance against rice blast infection. Overexpression of RACK1A enhances ROS production in rice seedlings. RACK1A was shown to interact with the N terminus of NADPH oxidase, RAR1, and SGT1, key regulators of plant disease resistance. These results suggest that RACK1A functions in rice innate immunity by interacting with multiple proteins in the Rac1[2].
immune complex.
Expression
In rice, RACK1A gene(OsRACK1A) plays an important role in controlling seed germination and its molecular mechanisms by generating a series of transgenic rice lines, of which OsRACK1A was either over-expressed or under-expressed. OsRACK1A positively regulated seed germination and negatively regulated the responses of seed germination to both exogenous ABA and H2O2. Inhibition of ABA biosynthesis had no enhancing effect on germination, whereas inhibition of ABA catabolism significantly suppressed germination. ABA inhibition on seed germination was almost fully recovered by exogenous H2O2 treatment. Quantitative analyses showed that endogenous ABA levels were significantly higher and H2O2 levels significantly lower in OsRACK1Adown regulated transgenic lines as compared with those in wildtype or OsRACK1A-up regulated lines. Quantitative real-time PCR analyses showed that the transcript levels of OsRbohs and amylase genes, RAmy1A and RAmy3D, were significantly lower in OsRACK1A-down regulated transgenic lines. It is concluded that OsRACK1A positively regulates seed germination by controlling endogenous levels of ABA and H2O2 and their interaction[1].
Evolution
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Labs working on this gene
- Department of Biotechnology, College of Bioscience and Biotechnology, Yangzhou University, Jiangsu, China.
- Department of Biological Engineering, Huanghuai University, Zhumadian City, Henan Province, China.
- Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.
- Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.
- Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.
- Agricultural and Veterinary Laboratories, Meiji Seika Kaisha, Kohoku-ku, Yokohama 222-8567, Japan.
- Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom.
References
- ↑ 1.0 1.1 Ayako Nakashima, Letian Chen, et al. RACK1 Functions in Rice Innate Immunity by Interacting with the Rac1 Immune Complex. The Plant Cell, Vol. 20: 2265–2279, August 2008.
- ↑ Dongping Zhang, Li Chen, et al. OsRACK1 Is Involved in Abscisic Acid- and H2O2-Mediated Signaling to Regulate Seed Germination in Rice (Oryza sativa, L.).PLOS ONE | www.plosone.org 2 May 2014 | Volume 9 | Issue 5 | e97120.
