IC4R020-RNA-Seq-2016-26752408

Project Title

Transcriptome Analysis of Salt Stress Responsiveness in the Seedlings of Dongxiang Wild Rice (Oryza rufipogon Griff.)

Seeds of Dongxiang wild rice (Oryza rufipogon Griff.; Dongxiang County, Jiangxi Province) and rice Xieqingzao B (O. sativa L. ssp. indica) were immersed in distilled water in the dark,and the uniformly germinated seeds were sown in 96-well plates supported by a plastic container. Seeds were grown in a growth chamber, as previously described [12]. The growth culture solution was renewed every 3 days. After the seedlings had been grown for 14 days, they were transferred on their 96-well plates into containers filled with 200 mM NaCl solution, or with control solution for 12 days. The seedlings were then recovered under normal solution for 3 days, and survival rates were calculated. The experiment was a randomized complete block design with three replications. For RNA-Seq analysis, 14-day-old seedlings of Dongxiang wild rice were grown with or without 200 mM NaCl treatment for 3 days and then the leaves (penultimate leaves) and total roots (separated from the culture solution and washed carefully) of these seedlings were collected and immediately frozen in liquid nitrogen, respectively. For RNA extraction from each treatment group, 10 plants were collected and mixed, to minimize the effect of transcriptome unevenness among plants.